Bacteroides phage B124-14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H2A0D5|H2A0D5_9CAUD Uncharacterized protein OS=Bacteroides phage B124-14 OX=1105171 GN=B124-14_030 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.2EE3 pKa = 3.97VIDD6 pKa = 4.26FKK8 pKa = 11.08IGDD11 pKa = 3.92EE12 pKa = 4.14YY13 pKa = 11.63KK14 pKa = 10.96EE15 pKa = 3.96GDD17 pKa = 3.3ILKK20 pKa = 10.06ARR22 pKa = 11.84EE23 pKa = 4.4GIYY26 pKa = 10.82LLVKK30 pKa = 9.61KK31 pKa = 6.44TTNKK35 pKa = 10.48NYY37 pKa = 10.06VEE39 pKa = 4.75CCTSCWFQNAPLDD52 pKa = 3.21VCMQMNCNAGNFYY65 pKa = 10.53FRR67 pKa = 11.84PFEE70 pKa = 4.09TYY72 pKa = 10.38KK73 pKa = 10.83EE74 pKa = 4.21GEE76 pKa = 4.32EE77 pKa = 4.32YY78 pKa = 11.02NVGDD82 pKa = 4.39LLKK85 pKa = 10.11IPKK88 pKa = 9.63PGDD91 pKa = 3.3PGKK94 pKa = 10.5FILSIVVEE102 pKa = 4.29DD103 pKa = 5.56DD104 pKa = 4.15IVDD107 pKa = 3.71EE108 pKa = 4.92FNNSCEE114 pKa = 3.91RR115 pKa = 11.84CVFKK119 pKa = 10.87DD120 pKa = 3.11WVYY123 pKa = 8.74PTDD126 pKa = 4.04DD127 pKa = 3.51CCSRR131 pKa = 11.84NRR133 pKa = 11.84CVDD136 pKa = 3.38CLRR139 pKa = 11.84DD140 pKa = 3.5INLDD144 pKa = 3.42DD145 pKa = 4.85VYY147 pKa = 11.0YY148 pKa = 10.88KK149 pKa = 10.7PLAEE153 pKa = 4.22VV154 pKa = 3.28

Molecular weight:
17.91 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H2A0G6|H2A0G6_9CAUD Uncharacterized protein OS=Bacteroides phage B124-14 OX=1105171 GN=B124-14_061 PE=4 SV=1
MM1 pKa = 7.4NRR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.89VKK7 pKa = 10.48KK8 pKa = 8.32NGYY11 pKa = 9.43RR12 pKa = 11.84IRR14 pKa = 11.84LTKK17 pKa = 10.51PSDD20 pKa = 3.1KK21 pKa = 10.28FVYY24 pKa = 10.61VSDD27 pKa = 3.31SLTYY31 pKa = 10.22EE32 pKa = 3.86RR33 pKa = 11.84RR34 pKa = 11.84KK35 pKa = 10.49KK36 pKa = 9.76EE37 pKa = 3.74GKK39 pKa = 9.36RR40 pKa = 11.84CYY42 pKa = 9.53TLYY45 pKa = 10.77CKK47 pKa = 10.37YY48 pKa = 11.21ASINYY53 pKa = 9.04LCVSRR58 pKa = 11.84KK59 pKa = 6.69QAKK62 pKa = 9.91SLMKK66 pKa = 10.62GFLLLWEE73 pKa = 4.38

Molecular weight:
8.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14468

37

2006

212.8

24.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.847 ± 0.501

1.686 ± 0.218

5.868 ± 0.217

7.838 ± 0.38

4.479 ± 0.232

6.089 ± 0.407

1.348 ± 0.135

6.884 ± 0.201

8.716 ± 0.546

8.052 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.198

5.847 ± 0.18

2.944 ± 0.236

3.401 ± 0.303

4.659 ± 0.265

6.393 ± 0.246

5.654 ± 0.389

6.4 ± 0.279

0.988 ± 0.088

4.555 ± 0.208

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski