Bacteroides phage B124-14
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H2A0D5|H2A0D5_9CAUD Uncharacterized protein OS=Bacteroides phage B124-14 OX=1105171 GN=B124-14_030 PE=4 SV=1
MM1 pKa = 7.59 KK2 pKa = 10.2 EE3 pKa = 3.97 VIDD6 pKa = 4.26 FKK8 pKa = 11.08 IGDD11 pKa = 3.92 EE12 pKa = 4.14 YY13 pKa = 11.63 KK14 pKa = 10.96 EE15 pKa = 3.96 GDD17 pKa = 3.3 ILKK20 pKa = 10.06 ARR22 pKa = 11.84 EE23 pKa = 4.4 GIYY26 pKa = 10.82 LLVKK30 pKa = 9.61 KK31 pKa = 6.44 TTNKK35 pKa = 10.48 NYY37 pKa = 10.06 VEE39 pKa = 4.75 CCTSCWFQNAPLDD52 pKa = 3.21 VCMQMNCNAGNFYY65 pKa = 10.53 FRR67 pKa = 11.84 PFEE70 pKa = 4.09 TYY72 pKa = 10.38 KK73 pKa = 10.83 EE74 pKa = 4.21 GEE76 pKa = 4.32 EE77 pKa = 4.32 YY78 pKa = 11.02 NVGDD82 pKa = 4.39 LLKK85 pKa = 10.11 IPKK88 pKa = 9.63 PGDD91 pKa = 3.3 PGKK94 pKa = 10.5 FILSIVVEE102 pKa = 4.29 DD103 pKa = 5.56 DD104 pKa = 4.15 IVDD107 pKa = 3.71 EE108 pKa = 4.92 FNNSCEE114 pKa = 3.91 RR115 pKa = 11.84 CVFKK119 pKa = 10.87 DD120 pKa = 3.11 WVYY123 pKa = 8.74 PTDD126 pKa = 4.04 DD127 pKa = 3.51 CCSRR131 pKa = 11.84 NRR133 pKa = 11.84 CVDD136 pKa = 3.38 CLRR139 pKa = 11.84 DD140 pKa = 3.5 INLDD144 pKa = 3.42 DD145 pKa = 4.85 VYY147 pKa = 11.0 YY148 pKa = 10.88 KK149 pKa = 10.7 PLAEE153 pKa = 4.22 VV154 pKa = 3.28
Molecular weight: 17.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.185
IPC2_protein 4.495
IPC_protein 4.418
Toseland 4.228
ProMoST 4.546
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.266
Rodwell 4.253
Grimsley 4.139
Solomon 4.368
Lehninger 4.329
Nozaki 4.482
DTASelect 4.685
Thurlkill 4.253
EMBOSS 4.279
Sillero 4.533
Patrickios 2.015
IPC_peptide 4.38
IPC2_peptide 4.52
IPC2.peptide.svr19 4.465
Protein with the highest isoelectric point:
>tr|H2A0G6|H2A0G6_9CAUD Uncharacterized protein OS=Bacteroides phage B124-14 OX=1105171 GN=B124-14_061 PE=4 SV=1
MM1 pKa = 7.4 NRR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 9.89 VKK7 pKa = 10.48 KK8 pKa = 8.32 NGYY11 pKa = 9.43 RR12 pKa = 11.84 IRR14 pKa = 11.84 LTKK17 pKa = 10.51 PSDD20 pKa = 3.1 KK21 pKa = 10.28 FVYY24 pKa = 10.61 VSDD27 pKa = 3.31 SLTYY31 pKa = 10.22 EE32 pKa = 3.86 RR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 10.49 KK36 pKa = 9.76 EE37 pKa = 3.74 GKK39 pKa = 9.36 RR40 pKa = 11.84 CYY42 pKa = 9.53 TLYY45 pKa = 10.77 CKK47 pKa = 10.37 YY48 pKa = 11.21 ASINYY53 pKa = 9.04 LCVSRR58 pKa = 11.84 KK59 pKa = 6.69 QAKK62 pKa = 9.91 SLMKK66 pKa = 10.62 GFLLLWEE73 pKa = 4.38
Molecular weight: 8.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.721
IPC_protein 9.867
Toseland 10.306
ProMoST 9.955
Dawson 10.482
Bjellqvist 10.145
Wikipedia 10.643
Rodwell 10.994
Grimsley 10.54
Solomon 10.511
Lehninger 10.482
Nozaki 10.306
DTASelect 10.131
Thurlkill 10.335
EMBOSS 10.701
Sillero 10.394
Patrickios 10.701
IPC_peptide 10.511
IPC2_peptide 8.99
IPC2.peptide.svr19 8.546
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
0
68
14468
37
2006
212.8
24.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.847 ± 0.501
1.686 ± 0.218
5.868 ± 0.217
7.838 ± 0.38
4.479 ± 0.232
6.089 ± 0.407
1.348 ± 0.135
6.884 ± 0.201
8.716 ± 0.546
8.052 ± 0.367
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.35 ± 0.198
5.847 ± 0.18
2.944 ± 0.236
3.401 ± 0.303
4.659 ± 0.265
6.393 ± 0.246
5.654 ± 0.389
6.4 ± 0.279
0.988 ± 0.088
4.555 ± 0.208
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here