Mycolicibacterium cosmeticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W9BL63|W9BL63_9MYCO Methylase putative OS=Mycolicibacterium cosmeticum OX=258533 GN=BN977_03920 PE=3 SV=1
MM1 pKa = 7.36FRR3 pKa = 11.84RR4 pKa = 11.84IAITAATAAALGLTVLAGAATASASSVDD32 pKa = 4.2DD33 pKa = 3.64QFLTNIDD40 pKa = 3.6AEE42 pKa = 4.94GIAFDD47 pKa = 3.99SARR50 pKa = 11.84AAISDD55 pKa = 3.36AHH57 pKa = 6.4LVCDD61 pKa = 4.83YY62 pKa = 11.02IADD65 pKa = 4.16GQSAASVGNDD75 pKa = 2.75ILDD78 pKa = 3.95NTDD81 pKa = 3.25LTTHH85 pKa = 5.65QAAVFVVEE93 pKa = 5.3SVSAYY98 pKa = 10.17CPGYY102 pKa = 8.99LTEE105 pKa = 5.04IGG107 pKa = 3.61

Molecular weight: 10.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W9AQI9|W9AQI9_9MYCO Uncharacterized protein OS=Mycolicibacterium cosmeticum OX=258533 GN=BN977_02565 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.38GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6271

0

6271

1990871

29

10376

317.5

33.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.592 ± 0.042

0.779 ± 0.008

6.234 ± 0.024

4.93 ± 0.027

3.055 ± 0.02

8.982 ± 0.029

2.255 ± 0.015

4.279 ± 0.02

2.089 ± 0.023

9.941 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.952 ± 0.012

2.143 ± 0.018

5.84 ± 0.03

2.94 ± 0.016

7.122 ± 0.034

5.102 ± 0.017

6.304 ± 0.026

8.839 ± 0.031

1.479 ± 0.012

2.142 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski