Palleronia marisminoris
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3664 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y5THL1|A0A1Y5THL1_9RHOB Aminoglycoside N(3)-acetyltransferase OS=Palleronia marisminoris OX=315423 GN=yokD PE=3 SV=1
MM1 pKa = 7.16 TFKK4 pKa = 8.65 TTLYY8 pKa = 9.64 TGAAMVALGTGMAQAQDD25 pKa = 3.61 FVFPKK30 pKa = 10.97 GEE32 pKa = 4.32 GAFSWDD38 pKa = 3.4 SLEE41 pKa = 4.5 EE42 pKa = 3.82 FASNHH47 pKa = 6.71 DD48 pKa = 3.86 YY49 pKa = 11.06 SGQSIDD55 pKa = 3.99 ITGPWTGADD64 pKa = 3.57 AEE66 pKa = 4.33 LMEE69 pKa = 4.69 SVVAYY74 pKa = 10.21 FEE76 pKa = 4.4 EE77 pKa = 4.49 ATGASVNYY85 pKa = 9.88 SGSDD89 pKa = 3.11 SFEE92 pKa = 3.74 QDD94 pKa = 2.53 IVISTEE100 pKa = 3.85 ANSAPNIAVFPQPGLLADD118 pKa = 4.5 LAQRR122 pKa = 11.84 GAITPLEE129 pKa = 4.47 PEE131 pKa = 3.62 TAEE134 pKa = 4.25 WIRR137 pKa = 11.84 EE138 pKa = 4.02 NYY140 pKa = 9.31 SAGEE144 pKa = 4.11 SWVDD148 pKa = 3.31 LASFEE153 pKa = 4.5 GEE155 pKa = 3.86 GGEE158 pKa = 3.94 EE159 pKa = 3.65 HH160 pKa = 7.24 LYY162 pKa = 10.93 AFPYY166 pKa = 10.05 KK167 pKa = 9.8 IDD169 pKa = 3.59 VKK171 pKa = 10.85 SLVWYY176 pKa = 10.29 VPEE179 pKa = 3.88 QFEE182 pKa = 4.18 EE183 pKa = 4.0 AGYY186 pKa = 9.5 EE187 pKa = 4.09 VPEE190 pKa = 4.31 TYY192 pKa = 10.29 EE193 pKa = 3.96 GLKK196 pKa = 10.62 EE197 pKa = 4.02 LTQQMADD204 pKa = 3.58 DD205 pKa = 6.02 GVTPWCIGLGSGAATGWPATDD226 pKa = 3.12 WVEE229 pKa = 4.87 DD230 pKa = 3.63 LMLRR234 pKa = 11.84 TATPEE239 pKa = 3.91 QYY241 pKa = 10.55 DD242 pKa = 3.14 AWVANDD248 pKa = 4.15 LAFDD252 pKa = 3.74 SPEE255 pKa = 3.76 VVNAIEE261 pKa = 4.26 EE262 pKa = 4.48 YY263 pKa = 11.24 GFFLQDD269 pKa = 3.11 GFVNGGRR276 pKa = 11.84 EE277 pKa = 3.91 AAATTDD283 pKa = 4.18 FRR285 pKa = 11.84 DD286 pKa = 3.76 SPSGLFQFPPEE297 pKa = 4.47 CYY299 pKa = 8.14 MHH301 pKa = 6.63 KK302 pKa = 10.01 QATFIPTFFPEE313 pKa = 4.54 GSEE316 pKa = 4.0 VGTDD320 pKa = 2.69 VDD322 pKa = 4.83 FFYY325 pKa = 11.09 FPAPEE330 pKa = 5.09 GGDD333 pKa = 3.25 AEE335 pKa = 4.41 QPVLGAGTMFAITNDD350 pKa = 3.56 SEE352 pKa = 4.2 PARR355 pKa = 11.84 GFIEE359 pKa = 4.23 YY360 pKa = 10.11 LQTPLAHH367 pKa = 6.86 EE368 pKa = 4.22 IWAAQGGLLTPHH380 pKa = 6.75 TGINPEE386 pKa = 4.0 VFGTDD391 pKa = 3.29 DD392 pKa = 3.5 QRR394 pKa = 11.84 ALNDD398 pKa = 3.39 ILLDD402 pKa = 3.47 ATTFRR407 pKa = 11.84 FDD409 pKa = 5.22 ASDD412 pKa = 3.81 LMPGEE417 pKa = 4.37 IGAGAFWTQMVEE429 pKa = 4.28 FTTGSQDD436 pKa = 3.18 AQATGEE442 pKa = 4.5 AIQSRR447 pKa = 11.84 WDD449 pKa = 3.34 QLSNN453 pKa = 3.42
Molecular weight: 49.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.567
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 3.961
Thurlkill 3.541
EMBOSS 3.579
Sillero 3.821
Patrickios 1.278
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A1Y5T7K7|A0A1Y5T7K7_9RHOB Sec-independent protein translocase protein TatB OS=Palleronia marisminoris OX=315423 GN=tatB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.3 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3664
0
3664
1119639
29
3005
305.6
33.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.471 ± 0.059
0.844 ± 0.012
6.267 ± 0.042
6.232 ± 0.035
3.596 ± 0.031
8.893 ± 0.039
2.053 ± 0.019
5.022 ± 0.029
2.559 ± 0.033
9.994 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.021
2.334 ± 0.025
5.25 ± 0.035
2.957 ± 0.024
7.328 ± 0.048
5.063 ± 0.028
5.519 ± 0.026
7.457 ± 0.033
1.401 ± 0.017
2.102 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here