Gordonia phage Kuwabara
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B9SXM6|A0A6B9SXM6_9CAUD Uncharacterized protein OS=Gordonia phage Kuwabara OX=2696370 GN=57 PE=4 SV=1
MM1 pKa = 7.43 TYY3 pKa = 8.71 STRR6 pKa = 11.84 HH7 pKa = 5.2 AVSAGGFRR15 pKa = 11.84 WRR17 pKa = 11.84 GPDD20 pKa = 3.37 YY21 pKa = 10.67 PYY23 pKa = 11.09 DD24 pKa = 3.84 LPGPGDD30 pKa = 4.36 ILPEE34 pKa = 5.07 DD35 pKa = 4.48 PDD37 pKa = 4.11 EE38 pKa = 5.63 DD39 pKa = 4.15 LPLYY43 pKa = 8.95 PNPAYY48 pKa = 10.13 GQQDD52 pKa = 3.61 PWRR55 pKa = 11.84 QPP57 pKa = 2.91
Molecular weight: 6.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.898
IPC2_protein 4.228
IPC_protein 4.101
Toseland 3.884
ProMoST 4.291
Dawson 4.101
Bjellqvist 4.266
Wikipedia 4.088
Rodwell 3.923
Grimsley 3.795
Solomon 4.088
Lehninger 4.05
Nozaki 4.24
DTASelect 4.507
Thurlkill 3.961
EMBOSS 4.088
Sillero 4.228
Patrickios 1.99
IPC_peptide 4.088
IPC2_peptide 4.19
IPC2.peptide.svr19 4.122
Protein with the highest isoelectric point:
>tr|A0A6B9SXP6|A0A6B9SXP6_9CAUD Uncharacterized protein OS=Gordonia phage Kuwabara OX=2696370 GN=84 PE=4 SV=1
MM1 pKa = 7.46 TEE3 pKa = 3.86 LRR5 pKa = 11.84 TRR7 pKa = 11.84 PPTGQPRR14 pKa = 11.84 WYY16 pKa = 9.13 PIPGFEE22 pKa = 4.3 NYY24 pKa = 9.45 EE25 pKa = 3.69 ISRR28 pKa = 11.84 FGHH31 pKa = 4.7 VRR33 pKa = 11.84 RR34 pKa = 11.84 GGRR37 pKa = 11.84 ILKK40 pKa = 6.97 TFKK43 pKa = 10.08 MKK45 pKa = 10.4 SGHH48 pKa = 6.6 LSISLCVNGVRR59 pKa = 11.84 YY60 pKa = 9.33 QRR62 pKa = 11.84 QVHH65 pKa = 5.76 RR66 pKa = 11.84 LVLEE70 pKa = 4.44 TFDD73 pKa = 4.17 GPAPEE78 pKa = 4.55 GMEE81 pKa = 3.76 ALHH84 pKa = 6.72 FNGIPDD90 pKa = 4.0 DD91 pKa = 3.94 NRR93 pKa = 11.84 IEE95 pKa = 3.99 NLRR98 pKa = 11.84 WGTRR102 pKa = 11.84 SANLRR107 pKa = 11.84 DD108 pKa = 3.75 AVRR111 pKa = 11.84 HH112 pKa = 4.18 GTYY115 pKa = 10.81 NNGHH119 pKa = 5.66 MRR121 pKa = 11.84 KK122 pKa = 6.53 THH124 pKa = 6.38 CPQNHH129 pKa = 7.15 PYY131 pKa = 10.25 SEE133 pKa = 4.47 EE134 pKa = 3.33 NTYY137 pKa = 10.31 RR138 pKa = 11.84 YY139 pKa = 9.61 RR140 pKa = 11.84 GRR142 pKa = 11.84 RR143 pKa = 11.84 HH144 pKa = 6.27 CKK146 pKa = 7.43 EE147 pKa = 3.64 CSRR150 pKa = 11.84 ARR152 pKa = 11.84 FRR154 pKa = 11.84 AYY156 pKa = 9.95 YY157 pKa = 10.37 AEE159 pKa = 4.5 RR160 pKa = 11.84 KK161 pKa = 9.33 AKK163 pKa = 10.64 RR164 pKa = 11.84 NGG166 pKa = 3.08
Molecular weight: 19.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.765
IPC_protein 10.672
Toseland 10.452
ProMoST 10.277
Dawson 10.628
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.73
Grimsley 10.716
Solomon 10.73
Lehninger 10.672
Nozaki 10.467
DTASelect 10.394
Thurlkill 10.496
EMBOSS 10.862
Sillero 10.57
Patrickios 10.335
IPC_peptide 10.716
IPC2_peptide 9.604
IPC2.peptide.svr19 8.392
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
94
0
94
17375
34
1810
184.8
20.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.471 ± 0.401
0.921 ± 0.136
6.429 ± 0.206
5.882 ± 0.356
2.406 ± 0.15
8.127 ± 0.441
1.945 ± 0.165
4.587 ± 0.182
3.367 ± 0.231
7.845 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.337 ± 0.137
3.085 ± 0.182
5.686 ± 0.252
3.868 ± 0.18
7.459 ± 0.371
5.853 ± 0.314
6.573 ± 0.325
7.568 ± 0.377
2.095 ± 0.155
2.498 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here