Amino acid dipepetide frequency for Gordonia phage Kuwabara

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.656AlaAla: 15.656 ± 1.254
0.403AlaCys: 0.403 ± 0.171
8.518AlaAsp: 8.518 ± 0.622
8.058AlaGlu: 8.058 ± 0.804
2.187AlaPhe: 2.187 ± 0.313
8.461AlaGly: 8.461 ± 0.833
1.899AlaHis: 1.899 ± 0.374
5.353AlaIle: 5.353 ± 0.542
4.72AlaLys: 4.72 ± 0.819
9.439AlaLeu: 9.439 ± 0.877
3.051AlaMet: 3.051 ± 0.566
3.396AlaAsn: 3.396 ± 0.589
5.18AlaPro: 5.18 ± 0.476
4.605AlaGln: 4.605 ± 0.51
8.346AlaArg: 8.346 ± 0.74
5.928AlaSer: 5.928 ± 0.548
6.331AlaThr: 6.331 ± 0.647
8.576AlaVal: 8.576 ± 0.905
2.13AlaTrp: 2.13 ± 0.343
2.475AlaTyr: 2.475 ± 0.352
0.0AlaXaa: 0.0 ± 0.0
Cys
0.806CysAla: 0.806 ± 0.243
0.058CysCys: 0.058 ± 0.067
0.691CysAsp: 0.691 ± 0.228
0.288CysGlu: 0.288 ± 0.113
0.115CysPhe: 0.115 ± 0.083
1.209CysGly: 1.209 ± 0.323
0.403CysHis: 0.403 ± 0.222
0.23CysIle: 0.23 ± 0.118
0.288CysLys: 0.288 ± 0.125
0.748CysLeu: 0.748 ± 0.204
0.173CysMet: 0.173 ± 0.092
0.115CysAsn: 0.115 ± 0.084
0.518CysPro: 0.518 ± 0.17
0.518CysGln: 0.518 ± 0.209
1.094CysArg: 1.094 ± 0.242
0.46CysSer: 0.46 ± 0.152
0.46CysThr: 0.46 ± 0.158
0.576CysVal: 0.576 ± 0.179
0.173CysTrp: 0.173 ± 0.108
0.288CysTyr: 0.288 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
7.482AspAla: 7.482 ± 0.612
0.403AspCys: 0.403 ± 0.143
3.338AspAsp: 3.338 ± 0.454
4.374AspGlu: 4.374 ± 0.533
1.496AspPhe: 1.496 ± 0.322
5.986AspGly: 5.986 ± 0.62
1.439AspHis: 1.439 ± 0.283
2.763AspIle: 2.763 ± 0.391
1.612AspLys: 1.612 ± 0.233
5.813AspLeu: 5.813 ± 0.5
1.036AspMet: 1.036 ± 0.259
1.151AspAsn: 1.151 ± 0.257
5.123AspPro: 5.123 ± 0.628
2.533AspGln: 2.533 ± 0.378
4.777AspArg: 4.777 ± 0.429
3.108AspSer: 3.108 ± 0.449
3.856AspThr: 3.856 ± 0.493
4.72AspVal: 4.72 ± 0.671
1.612AspTrp: 1.612 ± 0.305
1.669AspTyr: 1.669 ± 0.343
0.0AspXaa: 0.0 ± 0.0
Glu
6.677GluAla: 6.677 ± 0.637
0.806GluCys: 0.806 ± 0.23
2.187GluAsp: 2.187 ± 0.405
2.705GluGlu: 2.705 ± 0.462
2.015GluPhe: 2.015 ± 0.334
3.396GluGly: 3.396 ± 0.406
1.669GluHis: 1.669 ± 0.415
3.166GluIle: 3.166 ± 0.472
2.245GluLys: 2.245 ± 0.426
5.986GluLeu: 5.986 ± 0.776
1.496GluMet: 1.496 ± 0.302
1.381GluAsn: 1.381 ± 0.291
3.338GluPro: 3.338 ± 0.601
3.108GluGln: 3.108 ± 0.476
4.489GluArg: 4.489 ± 0.507
2.993GluSer: 2.993 ± 0.43
3.223GluThr: 3.223 ± 0.464
4.72GluVal: 4.72 ± 0.469
1.496GluTrp: 1.496 ± 0.316
1.727GluTyr: 1.727 ± 0.313
0.0GluXaa: 0.0 ± 0.0
Phe
2.763PheAla: 2.763 ± 0.329
0.345PheCys: 0.345 ± 0.14
2.015PheAsp: 2.015 ± 0.353
1.381PheGlu: 1.381 ± 0.287
0.691PhePhe: 0.691 ± 0.28
2.533PheGly: 2.533 ± 0.387
0.115PheHis: 0.115 ± 0.08
1.036PheIle: 1.036 ± 0.232
1.036PheLys: 1.036 ± 0.267
1.496PheLeu: 1.496 ± 0.391
0.288PheMet: 0.288 ± 0.118
0.978PheAsn: 0.978 ± 0.247
1.209PhePro: 1.209 ± 0.275
0.518PheGln: 0.518 ± 0.202
2.13PheArg: 2.13 ± 0.366
1.266PheSer: 1.266 ± 0.239
1.439PheThr: 1.439 ± 0.249
1.612PheVal: 1.612 ± 0.281
0.46PheTrp: 0.46 ± 0.165
0.748PheTyr: 0.748 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
6.907GlyAla: 6.907 ± 1.241
0.633GlyCys: 0.633 ± 0.209
4.835GlyAsp: 4.835 ± 0.647
4.374GlyGlu: 4.374 ± 0.52
2.648GlyPhe: 2.648 ± 0.355
8.116GlyGly: 8.116 ± 1.223
1.496GlyHis: 1.496 ± 0.256
3.569GlyIle: 3.569 ± 0.498
3.051GlyLys: 3.051 ± 0.511
6.619GlyLeu: 6.619 ± 0.654
2.36GlyMet: 2.36 ± 0.426
2.705GlyAsn: 2.705 ± 0.416
3.856GlyPro: 3.856 ± 0.409
2.475GlyGln: 2.475 ± 0.35
6.044GlyArg: 6.044 ± 0.542
5.295GlySer: 5.295 ± 0.763
5.525GlyThr: 5.525 ± 0.603
6.331GlyVal: 6.331 ± 0.581
2.59GlyTrp: 2.59 ± 0.4
1.842GlyTyr: 1.842 ± 0.354
0.0GlyXaa: 0.0 ± 0.0
His
1.842HisAla: 1.842 ± 0.278
0.576HisCys: 0.576 ± 0.246
0.806HisAsp: 0.806 ± 0.225
1.496HisGlu: 1.496 ± 0.303
0.576HisPhe: 0.576 ± 0.18
1.094HisGly: 1.094 ± 0.246
0.633HisHis: 0.633 ± 0.172
0.806HisIle: 0.806 ± 0.183
0.23HisLys: 0.23 ± 0.111
1.439HisLeu: 1.439 ± 0.271
0.288HisMet: 0.288 ± 0.107
0.403HisAsn: 0.403 ± 0.141
0.863HisPro: 0.863 ± 0.227
0.633HisGln: 0.633 ± 0.231
1.669HisArg: 1.669 ± 0.299
1.266HisSer: 1.266 ± 0.277
1.439HisThr: 1.439 ± 0.309
2.245HisVal: 2.245 ± 0.463
0.691HisTrp: 0.691 ± 0.261
0.46HisTyr: 0.46 ± 0.153
0.0HisXaa: 0.0 ± 0.0
Ile
5.928IleAla: 5.928 ± 0.573
0.403IleCys: 0.403 ± 0.152
3.856IleAsp: 3.856 ± 0.505
2.417IleGlu: 2.417 ± 0.485
0.633IlePhe: 0.633 ± 0.162
4.259IleGly: 4.259 ± 0.544
0.403IleHis: 0.403 ± 0.144
1.554IleIle: 1.554 ± 0.306
1.496IleLys: 1.496 ± 0.319
2.878IleLeu: 2.878 ± 0.454
1.036IleMet: 1.036 ± 0.285
1.554IleAsn: 1.554 ± 0.261
2.648IlePro: 2.648 ± 0.397
1.727IleGln: 1.727 ± 0.289
3.799IleArg: 3.799 ± 0.362
2.36IleSer: 2.36 ± 0.384
3.396IleThr: 3.396 ± 0.35
3.799IleVal: 3.799 ± 0.538
0.403IleTrp: 0.403 ± 0.133
1.324IleTyr: 1.324 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
3.741LysAla: 3.741 ± 0.48
0.46LysCys: 0.46 ± 0.163
1.727LysAsp: 1.727 ± 0.287
1.151LysGlu: 1.151 ± 0.204
0.748LysPhe: 0.748 ± 0.207
1.899LysGly: 1.899 ± 0.331
0.806LysHis: 0.806 ± 0.181
1.669LysIle: 1.669 ± 0.345
1.957LysLys: 1.957 ± 0.307
3.396LysLeu: 3.396 ± 0.471
0.518LysMet: 0.518 ± 0.157
1.151LysAsn: 1.151 ± 0.247
1.899LysPro: 1.899 ± 0.361
1.842LysGln: 1.842 ± 0.461
2.302LysArg: 2.302 ± 0.312
2.015LysSer: 2.015 ± 0.32
2.82LysThr: 2.82 ± 0.432
2.245LysVal: 2.245 ± 0.367
0.403LysTrp: 0.403 ± 0.175
0.921LysTyr: 0.921 ± 0.207
0.0LysXaa: 0.0 ± 0.0
Leu
9.094LeuAla: 9.094 ± 0.632
0.863LeuCys: 0.863 ± 0.256
5.583LeuAsp: 5.583 ± 0.584
4.892LeuGlu: 4.892 ± 0.495
1.842LeuPhe: 1.842 ± 0.384
6.274LeuGly: 6.274 ± 0.748
1.324LeuHis: 1.324 ± 0.289
3.051LeuIle: 3.051 ± 0.313
2.475LeuLys: 2.475 ± 0.418
6.389LeuLeu: 6.389 ± 0.583
2.015LeuMet: 2.015 ± 0.344
2.763LeuAsn: 2.763 ± 0.487
4.892LeuPro: 4.892 ± 0.618
2.36LeuGln: 2.36 ± 0.348
5.698LeuArg: 5.698 ± 0.592
4.835LeuSer: 4.835 ± 0.546
5.238LeuThr: 5.238 ± 0.534
6.159LeuVal: 6.159 ± 0.561
1.266LeuTrp: 1.266 ± 0.268
1.439LeuTyr: 1.439 ± 0.317
0.0LeuXaa: 0.0 ± 0.0
Met
2.82MetAla: 2.82 ± 0.381
0.23MetCys: 0.23 ± 0.104
1.496MetAsp: 1.496 ± 0.332
0.748MetGlu: 0.748 ± 0.264
0.345MetPhe: 0.345 ± 0.182
1.957MetGly: 1.957 ± 0.73
0.345MetHis: 0.345 ± 0.171
0.921MetIle: 0.921 ± 0.226
0.978MetLys: 0.978 ± 0.247
1.381MetLeu: 1.381 ± 0.262
0.576MetMet: 0.576 ± 0.167
0.576MetAsn: 0.576 ± 0.194
1.496MetPro: 1.496 ± 0.206
0.978MetGln: 0.978 ± 0.261
1.727MetArg: 1.727 ± 0.281
2.187MetSer: 2.187 ± 0.325
2.36MetThr: 2.36 ± 0.317
1.209MetVal: 1.209 ± 0.265
0.403MetTrp: 0.403 ± 0.168
0.633MetTyr: 0.633 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
4.202AsnAla: 4.202 ± 0.478
0.288AsnCys: 0.288 ± 0.122
1.842AsnAsp: 1.842 ± 0.34
1.612AsnGlu: 1.612 ± 0.308
0.46AsnPhe: 0.46 ± 0.183
2.935AsnGly: 2.935 ± 0.558
0.863AsnHis: 0.863 ± 0.224
1.151AsnIle: 1.151 ± 0.254
0.633AsnLys: 0.633 ± 0.177
2.245AsnLeu: 2.245 ± 0.4
0.46AsnMet: 0.46 ± 0.168
0.863AsnAsn: 0.863 ± 0.188
2.417AsnPro: 2.417 ± 0.353
1.209AsnGln: 1.209 ± 0.225
2.015AsnArg: 2.015 ± 0.393
1.381AsnSer: 1.381 ± 0.331
2.475AsnThr: 2.475 ± 0.38
2.648AsnVal: 2.648 ± 0.397
0.518AsnTrp: 0.518 ± 0.149
0.633AsnTyr: 0.633 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
6.216ProAla: 6.216 ± 0.557
0.576ProCys: 0.576 ± 0.185
4.489ProAsp: 4.489 ± 0.596
3.569ProGlu: 3.569 ± 0.462
1.439ProPhe: 1.439 ± 0.275
5.123ProGly: 5.123 ± 0.576
0.978ProHis: 0.978 ± 0.242
2.648ProIle: 2.648 ± 0.453
2.187ProLys: 2.187 ± 0.459
3.338ProLeu: 3.338 ± 0.379
1.036ProMet: 1.036 ± 0.234
2.13ProAsn: 2.13 ± 0.395
2.82ProPro: 2.82 ± 0.418
1.554ProGln: 1.554 ± 0.26
3.338ProArg: 3.338 ± 0.489
3.971ProSer: 3.971 ± 0.42
3.569ProThr: 3.569 ± 0.515
4.489ProVal: 4.489 ± 0.584
1.612ProTrp: 1.612 ± 0.291
1.784ProTyr: 1.784 ± 0.335
0.0ProXaa: 0.0 ± 0.0
Gln
4.374GlnAla: 4.374 ± 0.514
0.403GlnCys: 0.403 ± 0.18
1.842GlnAsp: 1.842 ± 0.339
2.36GlnGlu: 2.36 ± 0.314
0.748GlnPhe: 0.748 ± 0.2
2.935GlnGly: 2.935 ± 0.522
0.633GlnHis: 0.633 ± 0.187
2.302GlnIle: 2.302 ± 0.332
1.324GlnLys: 1.324 ± 0.248
3.396GlnLeu: 3.396 ± 0.558
1.151GlnMet: 1.151 ± 0.27
0.978GlnAsn: 0.978 ± 0.196
2.935GlnPro: 2.935 ± 0.309
2.187GlnGln: 2.187 ± 0.405
2.763GlnArg: 2.763 ± 0.431
2.072GlnSer: 2.072 ± 0.381
2.13GlnThr: 2.13 ± 0.364
2.475GlnVal: 2.475 ± 0.408
0.691GlnTrp: 0.691 ± 0.207
0.921GlnTyr: 0.921 ± 0.252
0.0GlnXaa: 0.0 ± 0.0
Arg
7.252ArgAla: 7.252 ± 0.68
0.863ArgCys: 0.863 ± 0.232
5.295ArgAsp: 5.295 ± 0.553
4.374ArgGlu: 4.374 ± 0.592
2.13ArgPhe: 2.13 ± 0.336
5.065ArgGly: 5.065 ± 0.517
2.36ArgHis: 2.36 ± 0.413
3.856ArgIle: 3.856 ± 0.541
2.59ArgLys: 2.59 ± 0.327
5.123ArgLeu: 5.123 ± 0.458
1.899ArgMet: 1.899 ± 0.328
2.417ArgAsn: 2.417 ± 0.315
3.338ArgPro: 3.338 ± 0.488
3.223ArgGln: 3.223 ± 0.392
6.331ArgArg: 6.331 ± 0.761
4.202ArgSer: 4.202 ± 0.449
4.662ArgThr: 4.662 ± 0.601
5.295ArgVal: 5.295 ± 0.668
1.957ArgTrp: 1.957 ± 0.343
2.36ArgTyr: 2.36 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
6.964SerAla: 6.964 ± 0.737
0.691SerCys: 0.691 ± 0.212
3.166SerAsp: 3.166 ± 0.407
3.396SerGlu: 3.396 ± 0.413
1.381SerPhe: 1.381 ± 0.263
6.619SerGly: 6.619 ± 0.915
0.806SerHis: 0.806 ± 0.241
2.648SerIle: 2.648 ± 0.442
1.899SerLys: 1.899 ± 0.279
4.202SerLeu: 4.202 ± 0.416
2.13SerMet: 2.13 ± 0.384
2.13SerAsn: 2.13 ± 0.402
2.705SerPro: 2.705 ± 0.318
2.417SerGln: 2.417 ± 0.463
4.317SerArg: 4.317 ± 0.522
3.453SerSer: 3.453 ± 0.541
3.799SerThr: 3.799 ± 0.543
3.971SerVal: 3.971 ± 0.612
0.978SerTrp: 0.978 ± 0.219
0.863SerTyr: 0.863 ± 0.213
0.0SerXaa: 0.0 ± 0.0
Thr
9.324ThrAla: 9.324 ± 0.797
0.403ThrCys: 0.403 ± 0.198
3.741ThrAsp: 3.741 ± 0.484
3.223ThrGlu: 3.223 ± 0.459
1.381ThrPhe: 1.381 ± 0.287
5.238ThrGly: 5.238 ± 0.585
1.266ThrHis: 1.266 ± 0.275
4.259ThrIle: 4.259 ± 0.478
1.784ThrLys: 1.784 ± 0.368
4.95ThrLeu: 4.95 ± 0.535
0.978ThrMet: 0.978 ± 0.256
1.957ThrAsn: 1.957 ± 0.296
4.317ThrPro: 4.317 ± 0.515
2.36ThrGln: 2.36 ± 0.33
3.971ThrArg: 3.971 ± 0.45
3.684ThrSer: 3.684 ± 0.559
4.432ThrThr: 4.432 ± 0.517
5.641ThrVal: 5.641 ± 0.742
0.978ThrTrp: 0.978 ± 0.19
1.842ThrTyr: 1.842 ± 0.334
0.0ThrXaa: 0.0 ± 0.0
Val
8.518ValAla: 8.518 ± 0.682
0.46ValCys: 0.46 ± 0.198
4.489ValAsp: 4.489 ± 0.612
5.698ValGlu: 5.698 ± 0.776
2.13ValPhe: 2.13 ± 0.331
4.432ValGly: 4.432 ± 0.631
0.921ValHis: 0.921 ± 0.205
2.878ValIle: 2.878 ± 0.399
1.957ValLys: 1.957 ± 0.324
6.101ValLeu: 6.101 ± 0.605
1.784ValMet: 1.784 ± 0.237
2.763ValAsn: 2.763 ± 0.338
4.605ValPro: 4.605 ± 0.534
2.648ValGln: 2.648 ± 0.376
6.044ValArg: 6.044 ± 0.706
5.295ValSer: 5.295 ± 0.682
5.468ValThr: 5.468 ± 0.611
6.274ValVal: 6.274 ± 0.947
1.381ValTrp: 1.381 ± 0.274
1.842ValTyr: 1.842 ± 0.315
0.0ValXaa: 0.0 ± 0.0
Trp
2.072TrpAla: 2.072 ± 0.374
0.23TrpCys: 0.23 ± 0.115
1.612TrpAsp: 1.612 ± 0.307
1.036TrpGlu: 1.036 ± 0.267
0.403TrpPhe: 0.403 ± 0.142
1.266TrpGly: 1.266 ± 0.273
0.46TrpHis: 0.46 ± 0.162
1.324TrpIle: 1.324 ± 0.248
0.518TrpLys: 0.518 ± 0.181
1.899TrpLeu: 1.899 ± 0.322
0.691TrpMet: 0.691 ± 0.185
0.863TrpAsn: 0.863 ± 0.192
1.094TrpPro: 1.094 ± 0.21
0.806TrpGln: 0.806 ± 0.226
1.439TrpArg: 1.439 ± 0.332
1.439TrpSer: 1.439 ± 0.299
1.381TrpThr: 1.381 ± 0.344
0.978TrpVal: 0.978 ± 0.216
0.748TrpTrp: 0.748 ± 0.233
0.691TrpTyr: 0.691 ± 0.199
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.072TyrAla: 2.072 ± 0.323
0.115TyrCys: 0.115 ± 0.087
2.763TyrAsp: 2.763 ± 0.415
1.669TyrGlu: 1.669 ± 0.338
0.691TyrPhe: 0.691 ± 0.19
2.475TyrGly: 2.475 ± 0.368
0.633TyrHis: 0.633 ± 0.169
0.691TyrIle: 0.691 ± 0.214
0.691TyrLys: 0.691 ± 0.194
1.612TyrLeu: 1.612 ± 0.366
0.345TyrMet: 0.345 ± 0.125
0.576TyrAsn: 0.576 ± 0.182
1.612TyrPro: 1.612 ± 0.392
0.978TyrGln: 0.978 ± 0.285
2.302TyrArg: 2.302 ± 0.345
1.324TyrSer: 1.324 ± 0.238
1.727TyrThr: 1.727 ± 0.351
1.727TyrVal: 1.727 ± 0.252
0.46TyrTrp: 0.46 ± 0.143
0.518TyrTyr: 0.518 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (17375 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski