Swinepox virus (strain Swine/Nebraska/17077-99/1999) (SWPV)
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 146 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q77G93|Q77G93_SWPV1 Serine/threonine-protein kinase OS=Swinepox virus (strain Swine/Nebraska/17077-99/1999) OX=300880 GN=SPV022 PE=3 SV=1
MM1 pKa = 7.81 GITHH5 pKa = 6.93 EE6 pKa = 4.48 LDD8 pKa = 2.7 IFLVSEE14 pKa = 5.07 DD15 pKa = 2.99 IAMKK19 pKa = 10.3 HH20 pKa = 5.38 VEE22 pKa = 3.95 LHH24 pKa = 6.2 KK25 pKa = 10.89 GNSYY29 pKa = 11.31 GCVLNIKK36 pKa = 9.7 SSCRR40 pKa = 11.84 KK41 pKa = 7.17 QMKK44 pKa = 9.88 IIFVLKK50 pKa = 10.21 PDD52 pKa = 3.05 WTEE55 pKa = 3.41 IDD57 pKa = 3.92 SIKK60 pKa = 9.57 PIQMEE65 pKa = 3.9 ADD67 pKa = 4.57 GIYY70 pKa = 9.96 IDD72 pKa = 3.7 VTIIKK77 pKa = 10.04 QSFYY81 pKa = 11.31 EE82 pKa = 4.58 VIYY85 pKa = 10.45 CSNFTIYY92 pKa = 10.64 GDD94 pKa = 3.69 SVINIFSDD102 pKa = 3.05 IDD104 pKa = 3.3 RR105 pKa = 11.84 CTKK108 pKa = 9.85 EE109 pKa = 4.23 KK110 pKa = 11.17 YY111 pKa = 10.11 PIININIDD119 pKa = 3.17 KK120 pKa = 10.35 KK121 pKa = 10.26 KK122 pKa = 10.82 YY123 pKa = 9.28 EE124 pKa = 4.49 IIEE127 pKa = 4.0 SGYY130 pKa = 8.58 TYY132 pKa = 10.3 IYY134 pKa = 9.52 IQSPIDD140 pKa = 3.66 KK141 pKa = 9.14 EE142 pKa = 4.7 DD143 pKa = 3.22 KK144 pKa = 9.12 LTYY147 pKa = 9.96 MRR149 pKa = 11.84 DD150 pKa = 3.75 DD151 pKa = 4.06 YY152 pKa = 11.81 VDD154 pKa = 4.25 EE155 pKa = 4.74 YY156 pKa = 11.68 NSSDD160 pKa = 4.22 DD161 pKa = 4.69 DD162 pKa = 4.79 CIDD165 pKa = 4.87 YY166 pKa = 11.05 YY167 pKa = 11.6 DD168 pKa = 5.3 CDD170 pKa = 5.26 YY171 pKa = 11.91 DD172 pKa = 6.32 DD173 pKa = 6.21 DD174 pKa = 5.46 DD175 pKa = 6.08 NNDD178 pKa = 3.87 AEE180 pKa = 5.63 DD181 pKa = 4.02 EE182 pKa = 4.26 DD183 pKa = 4.79 EE184 pKa = 4.21 EE185 pKa = 4.53
Molecular weight: 21.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 4.024
IPC_protein 4.037
Toseland 3.821
ProMoST 4.164
Dawson 4.024
Bjellqvist 4.202
Wikipedia 3.948
Rodwell 3.859
Grimsley 3.719
Solomon 4.012
Lehninger 3.973
Nozaki 4.126
DTASelect 4.38
Thurlkill 3.859
EMBOSS 3.961
Sillero 4.151
Patrickios 0.858
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.039
Protein with the highest isoelectric point:
>tr|Q8V3Q3|Q8V3Q3_SWPV1 SPV041 putative DNA-binding phosphoprotein OS=Swinepox virus (strain Swine/Nebraska/17077-99/1999) OX=300880 GN=SPV041 PE=4 SV=1
MM1 pKa = 7.63 TINTKK6 pKa = 11.11 DD7 pKa = 3.35 MFSLVSLTLLTIFMLACGGSLLFKK31 pKa = 10.26 TIAPHH36 pKa = 6.13 RR37 pKa = 11.84 LVITRR42 pKa = 11.84 SVTFNKK48 pKa = 9.46 VVNFLEE54 pKa = 4.37 YY55 pKa = 10.34 VAILIFIPGTITLYY69 pKa = 9.95 SAYY72 pKa = 10.41 VRR74 pKa = 11.84 TLFRR78 pKa = 5.87
Molecular weight: 8.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.266
IPC2_protein 9.648
IPC_protein 9.999
Toseland 10.028
ProMoST 10.101
Dawson 10.277
Bjellqvist 10.014
Wikipedia 10.496
Rodwell 10.57
Grimsley 10.379
Solomon 10.335
Lehninger 10.306
Nozaki 10.058
DTASelect 9.999
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.204
Patrickios 10.35
IPC_peptide 10.335
IPC2_peptide 8.99
IPC2.peptide.svr19 8.521
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
146
0
146
46268
53
1959
316.9
36.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.565 ± 0.113
2.129 ± 0.112
6.49 ± 0.129
4.932 ± 0.105
4.919 ± 0.142
3.536 ± 0.133
2.051 ± 0.062
12.052 ± 0.22
8.17 ± 0.157
8.667 ± 0.169
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.516 ± 0.08
7.884 ± 0.156
2.857 ± 0.112
2.053 ± 0.079
3.776 ± 0.118
7.962 ± 0.158
5.762 ± 0.174
5.209 ± 0.114
0.676 ± 0.049
5.792 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here