Laetiporus sulphureus 93-53
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13712 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A165HBS7|A0A165HBS7_9APHY Telomere_reg-2 domain-containing protein OS=Laetiporus sulphureus 93-53 OX=1314785 GN=LAESUDRAFT_740990 PE=3 SV=1
MM1 pKa = 7.47 TEE3 pKa = 4.25 STSQSPSLLAMARR16 pKa = 11.84 NKK18 pKa = 10.4 LLSTSVNGVKK28 pKa = 10.35 DD29 pKa = 3.48 SSSLHH34 pKa = 5.25 RR35 pKa = 11.84 WVLLRR40 pKa = 11.84 NSLVQPHH47 pKa = 6.19 TPAPIATPEE56 pKa = 4.23 KK57 pKa = 10.2 ADD59 pKa = 3.15 VPYY62 pKa = 10.6 VYY64 pKa = 10.28 RR65 pKa = 11.84 RR66 pKa = 11.84 VEE68 pKa = 5.22 DD69 pKa = 3.66 EE70 pKa = 4.46 GCDD73 pKa = 3.57 QEE75 pKa = 5.2 EE76 pKa = 4.16 EE77 pKa = 4.62 VAFMFPDD84 pKa = 4.2 PDD86 pKa = 3.79 ALSRR90 pKa = 11.84 DD91 pKa = 3.48 AGVCGSEE98 pKa = 4.2 NDD100 pKa = 3.63 WLDD103 pKa = 3.93 SLLEE107 pKa = 4.34 DD108 pKa = 5.26 LGEE111 pKa = 5.62 DD112 pKa = 3.76 DD113 pKa = 6.44 DD114 pKa = 6.58 LDD116 pKa = 4.93 AEE118 pKa = 4.4 EE119 pKa = 5.6 GGLEE123 pKa = 4.17 TSMSGAPPDD132 pKa = 4.41 EE133 pKa = 5.26 DD134 pKa = 5.72 DD135 pKa = 4.67 EE136 pKa = 4.73 PLSPLYY142 pKa = 11.11 SPMSSSDD149 pKa = 3.42 NLVDD153 pKa = 3.6 QSSFYY158 pKa = 10.91 VNPHH162 pKa = 7.54 AIPIPYY168 pKa = 8.47 PIPYY172 pKa = 9.42 PPVRR176 pKa = 11.84 PSLVSAWYY184 pKa = 10.24 GLDD187 pKa = 3.46 SSPDD191 pKa = 3.54 SLLEE195 pKa = 4.04 ASSPLYY201 pKa = 10.36 HH202 pKa = 7.69 DD203 pKa = 4.54 PLPYY207 pKa = 10.42 YY208 pKa = 10.67 DD209 pKa = 4.92 VDD211 pKa = 3.85 DD212 pKa = 4.78 TEE214 pKa = 5.15 DD215 pKa = 4.25 LPVPDD220 pKa = 5.83 AIEE223 pKa = 4.42 DD224 pKa = 3.94 TSDD227 pKa = 4.05 DD228 pKa = 4.06 EE229 pKa = 5.53 SDD231 pKa = 3.98 APATPFDD238 pKa = 4.23 HH239 pKa = 6.4 STSSICPLDD248 pKa = 4.09 PASVPLPSEE257 pKa = 3.81 RR258 pKa = 11.84 RR259 pKa = 11.84 RR260 pKa = 11.84 RR261 pKa = 11.84 SHH263 pKa = 6.26 SRR265 pKa = 11.84 QPQVYY270 pKa = 9.36 VDD272 pKa = 3.6 TDD274 pKa = 3.16 DD275 pKa = 4.54 SYY277 pKa = 11.82 FYY279 pKa = 10.67 PFGLDD284 pKa = 3.46 PLPSHH289 pKa = 7.32 VDD291 pKa = 3.58 NPVHH295 pKa = 6.8 AARR298 pKa = 11.84 VYY300 pKa = 10.49 GPSVYY305 pKa = 9.37 QQCC308 pKa = 3.84
Molecular weight: 33.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.706
Grimsley 3.567
Solomon 3.872
Lehninger 3.834
Nozaki 3.986
DTASelect 4.253
Thurlkill 3.719
EMBOSS 3.821
Sillero 4.012
Patrickios 1.087
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A0A165DLL6|A0A165DLL6_9APHY Clavaminate synthase-like protein OS=Laetiporus sulphureus 93-53 OX=1314785 GN=LAESUDRAFT_715172 PE=4 SV=1
MM1 pKa = 7.8 SPFLLSALFILHH13 pKa = 6.96 LPHH16 pKa = 6.89 SARR19 pKa = 11.84 LSRR22 pKa = 11.84 FRR24 pKa = 11.84 SRR26 pKa = 11.84 TSSPQSALSSHH37 pKa = 6.85 LPAQARR43 pKa = 11.84 PPLAKK48 pKa = 9.92 RR49 pKa = 11.84 RR50 pKa = 11.84 ALPVSQTPP58 pKa = 3.21
Molecular weight: 6.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13707
5
13712
5380101
49
5037
392.4
43.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.944 ± 0.019
1.379 ± 0.01
5.534 ± 0.014
6.144 ± 0.022
3.545 ± 0.012
6.226 ± 0.019
2.706 ± 0.011
4.78 ± 0.015
4.328 ± 0.02
9.23 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.299 ± 0.007
3.174 ± 0.01
6.287 ± 0.027
3.74 ± 0.015
6.553 ± 0.021
8.666 ± 0.028
5.857 ± 0.016
6.483 ± 0.014
1.438 ± 0.009
2.688 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here