Rice latent virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus

Average proteome isoelectric point is 7.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0WZA5|A0A2D0WZA5_9GEMI Capsid protein OS=Rice latent virus 1 OX=2012856 PE=3 SV=1
MM1 pKa = 7.99ADD3 pKa = 3.99PSTSHH8 pKa = 5.68QFSVRR13 pKa = 11.84TKK15 pKa = 10.32YY16 pKa = 10.6FFLTYY21 pKa = 8.83PKK23 pKa = 10.48CPVDD27 pKa = 3.32PWEE30 pKa = 4.31LLHH33 pKa = 7.13YY34 pKa = 10.67LLTLLHH40 pKa = 6.54SRR42 pKa = 11.84VPIYY46 pKa = 10.51ILVTRR51 pKa = 11.84EE52 pKa = 3.75SHH54 pKa = 6.98KK55 pKa = 11.13DD56 pKa = 3.41GSFHH60 pKa = 7.46LHH62 pKa = 6.01TLLQLSNRR70 pKa = 11.84LEE72 pKa = 4.37TKK74 pKa = 10.25DD75 pKa = 3.94SRR77 pKa = 11.84FFDD80 pKa = 3.85YY81 pKa = 10.76QSYY84 pKa = 10.5HH85 pKa = 6.91PNCQPAQNCKK95 pKa = 7.13QVRR98 pKa = 11.84DD99 pKa = 4.24YY100 pKa = 9.72ITKK103 pKa = 9.91PGTVSIAEE111 pKa = 3.71WGEE114 pKa = 3.87FYY116 pKa = 10.59NAKK119 pKa = 9.98PFSKK123 pKa = 9.3KK124 pKa = 6.7TLKK127 pKa = 10.33RR128 pKa = 11.84RR129 pKa = 11.84APGSDD134 pKa = 2.84TKK136 pKa = 10.05DD137 pKa = 2.75TKK139 pKa = 9.64MRR141 pKa = 11.84KK142 pKa = 9.06IISSSTSKK150 pKa = 9.8TEE152 pKa = 3.7YY153 pKa = 10.61LSMVRR158 pKa = 11.84QTFPFDD164 pKa = 2.8WATRR168 pKa = 11.84LAQFEE173 pKa = 4.35YY174 pKa = 10.47SAEE177 pKa = 4.09KK178 pKa = 10.68LFPTTVTPYY187 pKa = 10.62ASSVPTDD194 pKa = 3.25QLRR197 pKa = 11.84CSEE200 pKa = 4.84QLSQWATEE208 pKa = 4.17NIKK211 pKa = 9.88TYY213 pKa = 7.19TVCEE217 pKa = 3.85EE218 pKa = 4.96VYY220 pKa = 9.35TQTRR224 pKa = 11.84QNMDD228 pKa = 3.65YY229 pKa = 11.28LDD231 pKa = 3.82VTTAEE236 pKa = 4.52YY237 pKa = 9.99LQHH240 pKa = 6.62VHH242 pKa = 7.37DD243 pKa = 4.46LTSRR247 pKa = 11.84MLQQPPEE254 pKa = 4.88DD255 pKa = 4.0IPPYY259 pKa = 9.79MYY261 pKa = 10.86ADD263 pKa = 3.37QPEE266 pKa = 4.25QEE268 pKa = 4.21RR269 pKa = 11.84QPGQEE274 pKa = 3.85ASGSTTIGTDD284 pKa = 2.26WWTSPLMTPPHH295 pKa = 5.55NTMLL299 pKa = 4.31

Molecular weight: 34.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0WZC9|A0A2D0WZC9_9GEMI Replication-associated protein OS=Rice latent virus 1 OX=2012856 PE=3 SV=1
MM1 pKa = 7.76PGGSKK6 pKa = 10.08RR7 pKa = 11.84ASSGKK12 pKa = 10.13DD13 pKa = 2.87GSRR16 pKa = 11.84PKK18 pKa = 10.08RR19 pKa = 11.84RR20 pKa = 11.84KK21 pKa = 10.04GEE23 pKa = 3.73PEE25 pKa = 3.33AAFRR29 pKa = 11.84ARR31 pKa = 11.84ILGWASSQFGRR42 pKa = 11.84RR43 pKa = 11.84VYY45 pKa = 10.91GGVGSSQSAPLQVRR59 pKa = 11.84TYY61 pKa = 10.48KK62 pKa = 10.04WEE64 pKa = 4.29NPDD67 pKa = 3.25KK68 pKa = 11.01SAIAIDD74 pKa = 3.84TGSIWIVNTLDD85 pKa = 3.95AGNGDD90 pKa = 4.34DD91 pKa = 4.91QRR93 pKa = 11.84HH94 pKa = 3.87THH96 pKa = 5.3KK97 pKa = 9.91TIIYY101 pKa = 9.83KK102 pKa = 9.32MLFQGTMWINDD113 pKa = 3.42ATSAVCGPLTVHH125 pKa = 6.61FWLVYY130 pKa = 9.55DD131 pKa = 4.63AAPKK135 pKa = 10.52GVLPALTDD143 pKa = 3.18IFEE146 pKa = 4.65EE147 pKa = 5.08GFSGLGSTWVVNRR160 pKa = 11.84DD161 pKa = 3.03NVHH164 pKa = 5.9RR165 pKa = 11.84FVVKK169 pKa = 10.39RR170 pKa = 11.84RR171 pKa = 11.84WQMRR175 pKa = 11.84LMTNGQKK182 pKa = 10.18PYY184 pKa = 8.58YY185 pKa = 8.51TNRR188 pKa = 11.84SGNGDD193 pKa = 3.58PTSVTIRR200 pKa = 11.84DD201 pKa = 3.44FRR203 pKa = 11.84KK204 pKa = 9.6FFTKK208 pKa = 10.36LRR210 pKa = 11.84TATEE214 pKa = 4.26WKK216 pKa = 10.26NSATGGIGDD225 pKa = 3.8IKK227 pKa = 10.77KK228 pKa = 10.49GALYY232 pKa = 10.12LCAMCSQAPEE242 pKa = 4.08GSARR246 pKa = 11.84AINLGVKK253 pKa = 10.21FRR255 pKa = 11.84GQSRR259 pKa = 11.84IYY261 pKa = 9.56FKK263 pKa = 10.81CTGNQQ268 pKa = 3.0

Molecular weight: 29.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1009

88

354

252.3

28.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.748 ± 0.884

1.982 ± 0.344

5.055 ± 0.288

4.262 ± 0.595

4.658 ± 0.544

5.847 ± 1.787

2.478 ± 0.535

4.163 ± 0.492

6.046 ± 0.781

7.334 ± 0.94

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.883 ± 0.366

3.271 ± 0.463

6.145 ± 0.687

4.658 ± 0.734

6.046 ± 0.674

8.127 ± 0.314

9.514 ± 0.845

5.55 ± 1.152

2.279 ± 0.278

4.955 ± 0.806
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski