Enterobacter lignolyticus (strain SCF1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Pluralibacter; [Enterobacter] lignolyticus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4393 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3G2K8|E3G2K8_ENTLS Prophage CP4-57 regulatory OS=Enterobacter lignolyticus (strain SCF1) OX=701347 GN=Entcl_0672 PE=4 SV=1
MM1 pKa = 8.25RR2 pKa = 11.84YY3 pKa = 7.86TSRR6 pKa = 11.84LSLLSHH12 pKa = 7.36CILLSLTSFAVQASATTPSVPCAGGDD38 pKa = 3.27ITQTCGLSVYY48 pKa = 9.65TDD50 pKa = 3.09NNFYY54 pKa = 10.65QGSGDD59 pKa = 3.52KK60 pKa = 10.76DD61 pKa = 3.17AVMAQATATNIYY73 pKa = 9.34MDD75 pKa = 4.04GNRR78 pKa = 11.84RR79 pKa = 11.84AGDD82 pKa = 3.66TQSLIVNGTDD92 pKa = 2.92MSGAYY97 pKa = 8.98IQASKK102 pKa = 11.02GGTVNITMIDD112 pKa = 3.64GASADD117 pKa = 4.05MIEE120 pKa = 5.09SGDD123 pKa = 3.77AGQTTNTAITLDD135 pKa = 3.72DD136 pKa = 4.01AVLKK140 pKa = 11.34GEE142 pKa = 5.03DD143 pKa = 3.87DD144 pKa = 4.3SIAYY148 pKa = 8.41TPGDD152 pKa = 3.45AAGDD156 pKa = 3.75KK157 pKa = 10.69AYY159 pKa = 10.68MMGSAIFIDD168 pKa = 4.38PLDD171 pKa = 4.36AGNHH175 pKa = 4.91SVAITDD181 pKa = 3.7GSLVQGSIVSAGAGVQTIALNNSSLVNGGIYY212 pKa = 9.81TGSNASATTIALTNSVLDD230 pKa = 4.01ASQSEE235 pKa = 4.57IAQQAGEE242 pKa = 4.18IVAGLNEE249 pKa = 4.38KK250 pKa = 10.65GVGPFSNINVQEE262 pKa = 3.99YY263 pKa = 10.39DD264 pKa = 3.5DD265 pKa = 4.42VGIAVYY271 pKa = 9.99GKK273 pKa = 10.14SAMALSLNNSTLTGDD288 pKa = 3.16IALLNEE294 pKa = 4.67GNDD297 pKa = 3.77AGPGSISVTMANNTTVNGDD316 pKa = 3.4MTLVGQSSNTITLTDD331 pKa = 3.99DD332 pKa = 3.18STLNGDD338 pKa = 2.97VDD340 pKa = 3.84AANNSGNTALTLDD353 pKa = 3.64NYY355 pKa = 11.3SRR357 pKa = 11.84INGNIALGSGNDD369 pKa = 3.95TVTLTHH375 pKa = 7.29DD376 pKa = 3.58SQVNGDD382 pKa = 3.51VDD384 pKa = 4.31AGAGDD389 pKa = 4.18DD390 pKa = 4.11TLSMDD395 pKa = 4.02EE396 pKa = 4.62GSTISGQISDD406 pKa = 4.43FEE408 pKa = 4.71TVNTAGNNNIKK419 pKa = 9.57TDD421 pKa = 4.68AINDD425 pKa = 3.64TSTWNLQNGSQLTAQEE441 pKa = 4.34TGSNVQVNMSADD453 pKa = 3.58SYY455 pKa = 11.47VDD457 pKa = 3.58LGTVTGTNDD466 pKa = 3.65EE467 pKa = 4.25IVVNSVTAASVNQQDD482 pKa = 3.76QEE484 pKa = 4.14IGTFTTAGDD493 pKa = 4.09APQTAAAAAFSNGEE507 pKa = 3.86QQVEE511 pKa = 4.53SRR513 pKa = 11.84SGAYY517 pKa = 9.86NYY519 pKa = 10.84SNSLDD524 pKa = 3.54VVAAQSALLTASDD537 pKa = 3.8TQSWDD542 pKa = 3.08ILFSSSRR549 pKa = 11.84SSLASDD555 pKa = 3.71VQGLMAGLDD564 pKa = 3.49AAEE567 pKa = 4.18QAGQLITDD575 pKa = 5.52DD576 pKa = 4.21IANHH580 pKa = 5.12MTQIHH585 pKa = 5.76LQNLRR590 pKa = 11.84GHH592 pKa = 5.58VVGGAQLWGDD602 pKa = 3.72FLYY605 pKa = 11.16QNGDD609 pKa = 3.02VSDD612 pKa = 4.23DD613 pKa = 3.18VDD615 pKa = 4.09YY616 pKa = 11.95NSITQGAQGGVDD628 pKa = 3.2WTIPLANGDD637 pKa = 4.11SLTAGMALGWTRR649 pKa = 11.84SRR651 pKa = 11.84VEE653 pKa = 4.32DD654 pKa = 3.45NSGGEE659 pKa = 4.02NRR661 pKa = 11.84FKK663 pKa = 10.79DD664 pKa = 3.86TVYY667 pKa = 10.78GNYY670 pKa = 9.85YY671 pKa = 10.01SLYY674 pKa = 10.6GGWQQALRR682 pKa = 11.84DD683 pKa = 4.2SRR685 pKa = 11.84WGMFIDD691 pKa = 5.03GSVSYY696 pKa = 11.79GDD698 pKa = 3.39MRR700 pKa = 11.84YY701 pKa = 10.04SLSAQNVTGNTSGMTEE717 pKa = 4.06ALSGSTRR724 pKa = 11.84GDD726 pKa = 3.33LYY728 pKa = 11.07VAQARR733 pKa = 11.84TGVNILLPAEE743 pKa = 4.41TVLQPYY749 pKa = 7.69VTLGWDD755 pKa = 2.83KK756 pKa = 11.28AAADD760 pKa = 5.16GYY762 pKa = 10.87ADD764 pKa = 3.97SEE766 pKa = 4.49IAFSDD771 pKa = 3.59SHH773 pKa = 6.91VSSWNSSVGVHH784 pKa = 6.87LSTTVACLAKK794 pKa = 9.8NTTLTPWLDD803 pKa = 3.16MRR805 pKa = 11.84YY806 pKa = 9.66QGQFSDD812 pKa = 3.7NTDD815 pKa = 2.41IRR817 pKa = 11.84AADD820 pKa = 3.52YY821 pKa = 11.26HH822 pKa = 5.88NTDD825 pKa = 2.76GHH827 pKa = 6.07NLSLGIFGAGISATIAHH844 pKa = 5.89SVALNTGVYY853 pKa = 10.23FGTGDD858 pKa = 3.24VDD860 pKa = 4.08NNASVQAGISYY871 pKa = 10.26RR872 pKa = 11.84FF873 pKa = 3.33

Molecular weight: 90.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3G6E6|E3G6E6_ENTLS Thiol:disulfide interchange protein OS=Enterobacter lignolyticus (strain SCF1) OX=701347 GN=Entcl_0997 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSKK46 pKa = 10.99

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4393

0

4393

1420044

30

2884

323.3

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.218 ± 0.051

1.119 ± 0.013

5.206 ± 0.029

5.351 ± 0.035

3.856 ± 0.024

7.59 ± 0.043

2.267 ± 0.021

5.699 ± 0.031

3.962 ± 0.028

10.899 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.016

3.621 ± 0.028

4.497 ± 0.022

4.433 ± 0.029

5.787 ± 0.035

5.874 ± 0.025

5.329 ± 0.023

7.212 ± 0.03

1.532 ± 0.016

2.751 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski