Amino acid dipepetide frequency for Rhodococcus sp. RD6.2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.266AlaAla: 20.266 ± 0.173	0.897AlaCys: 0.897 ± 0.023	8.655AlaAsp: 8.655 ± 0.086	7.569AlaGlu: 7.569 ± 0.087	3.459AlaPhe: 3.459 ± 0.055	12.113AlaGly: 12.113 ± 0.11	2.574AlaHis: 2.574 ± 0.039	4.906AlaIle: 4.906 ± 0.064	2.629AlaLys: 2.629 ± 0.053	13.42AlaLeu: 13.42 ± 0.124	2.69AlaMet: 2.69 ± 0.049	2.382AlaAsn: 2.382 ± 0.043	6.504AlaPro: 6.504 ± 0.082	3.64AlaGln: 3.64 ± 0.051	8.824AlaArg: 8.824 ± 0.089	6.427AlaSer: 6.427 ± 0.063	7.781AlaThr: 7.781 ± 0.077	12.445AlaVal: 12.445 ± 0.105	1.551AlaTrp: 1.551 ± 0.031	2.311AlaTyr: 2.311 ± 0.038	0.001AlaXaa: 0.001 ± 0.001
Cys	0.985CysAla: 0.985 ± 0.026	0.099CysCys: 0.099 ± 0.007	0.447CysAsp: 0.447 ± 0.016	0.382CysGlu: 0.382 ± 0.016	0.23CysPhe: 0.23 ± 0.011	0.904CysGly: 0.904 ± 0.026	0.185CysHis: 0.185 ± 0.011	0.238CysIle: 0.238 ± 0.011	0.108CysLys: 0.108 ± 0.008	0.612CysLeu: 0.612 ± 0.022	0.132CysMet: 0.132 ± 0.009	0.149CysAsn: 0.149 ± 0.009	0.484CysPro: 0.484 ± 0.018	0.163CysGln: 0.163 ± 0.009	0.563CysArg: 0.563 ± 0.02	0.492CysSer: 0.492 ± 0.017	0.526CysThr: 0.526 ± 0.018	0.635CysVal: 0.635 ± 0.02	0.118CysTrp: 0.118 ± 0.009	0.147CysTyr: 0.147 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.595AspAla: 8.595 ± 0.096	0.429AspCys: 0.429 ± 0.017	4.106AspAsp: 4.106 ± 0.057	4.085AspGlu: 4.085 ± 0.06	1.68AspPhe: 1.68 ± 0.034	6.424AspGly: 6.424 ± 0.061	1.513AspHis: 1.513 ± 0.031	2.206AspIle: 2.206 ± 0.035	1.078AspLys: 1.078 ± 0.031	6.845AspLeu: 6.845 ± 0.093	0.926AspMet: 0.926 ± 0.024	1.088AspAsn: 1.088 ± 0.022	4.582AspPro: 4.582 ± 0.06	1.658AspGln: 1.658 ± 0.031	5.359AspArg: 5.359 ± 0.067	3.092AspSer: 3.092 ± 0.041	3.593AspThr: 3.593 ± 0.049	5.61AspVal: 5.61 ± 0.062	0.963AspTrp: 0.963 ± 0.027	1.216AspTyr: 1.216 ± 0.031	0.002AspXaa: 0.002 ± 0.001
Glu	5.915GluAla: 5.915 ± 0.072	0.356GluCys: 0.356 ± 0.016	2.565GluAsp: 2.565 ± 0.038	2.547GluGlu: 2.547 ± 0.049	1.92GluPhe: 1.92 ± 0.032	3.589GluGly: 3.589 ± 0.046	1.458GluHis: 1.458 ± 0.031	2.572GluIle: 2.572 ± 0.04	1.296GluLys: 1.296 ± 0.035	6.365GluLeu: 6.365 ± 0.071	1.057GluMet: 1.057 ± 0.027	1.247GluAsn: 1.247 ± 0.025	2.996GluPro: 2.996 ± 0.046	2.132GluGln: 2.132 ± 0.043	4.644GluArg: 4.644 ± 0.064	3.272GluSer: 3.272 ± 0.047	2.66GluThr: 2.66 ± 0.038	4.693GluVal: 4.693 ± 0.054	0.802GluTrp: 0.802 ± 0.024	1.133GluTyr: 1.133 ± 0.027	0.001GluXaa: 0.001 ± 0.001
Phe	4.043PheAla: 4.043 ± 0.056	0.253PheCys: 0.253 ± 0.011	2.421PheAsp: 2.421 ± 0.053	1.644PheGlu: 1.644 ± 0.032	0.863PhePhe: 0.863 ± 0.025	3.394PheGly: 3.394 ± 0.055	0.602PheHis: 0.602 ± 0.02	0.79PheIle: 0.79 ± 0.02	0.43PheLys: 0.43 ± 0.018	2.537PheLeu: 2.537 ± 0.05	0.447PheMet: 0.447 ± 0.018	0.583PheAsn: 0.583 ± 0.019	1.386PhePro: 1.386 ± 0.031	0.655PheGln: 0.655 ± 0.024	1.699PheArg: 1.699 ± 0.035	1.532PheSer: 1.532 ± 0.032	2.06PheThr: 2.06 ± 0.037	2.822PheVal: 2.822 ± 0.05	0.411PheTrp: 0.411 ± 0.016	0.595PheTyr: 0.595 ± 0.02	0.001PheXaa: 0.001 ± 0.001
Gly	10.737GlyAla: 10.737 ± 0.108	0.776GlyCys: 0.776 ± 0.023	5.466GlyAsp: 5.466 ± 0.062	4.652GlyGlu: 4.652 ± 0.059	3.088GlyPhe: 3.088 ± 0.043	8.33GlyGly: 8.33 ± 0.091	2.118GlyHis: 2.118 ± 0.039	4.078GlyIle: 4.078 ± 0.058	2.125GlyLys: 2.125 ± 0.038	8.68GlyLeu: 8.68 ± 0.083	2.126GlyMet: 2.126 ± 0.038	1.905GlyAsn: 1.905 ± 0.038	4.521GlyPro: 4.521 ± 0.057	2.472GlyGln: 2.472 ± 0.043	6.925GlyArg: 6.925 ± 0.064	5.679GlySer: 5.679 ± 0.062	6.025GlyThr: 6.025 ± 0.066	8.361GlyVal: 8.361 ± 0.089	1.593GlyTrp: 1.593 ± 0.035	2.236GlyTyr: 2.236 ± 0.035	0.003GlyXaa: 0.003 ± 0.001
His	2.401HisAla: 2.401 ± 0.037	0.214HisCys: 0.214 ± 0.01	1.408HisAsp: 1.408 ± 0.028	1.089HisGlu: 1.089 ± 0.025	0.626HisPhe: 0.626 ± 0.02	2.074HisGly: 2.074 ± 0.036	0.689HisHis: 0.689 ± 0.023	0.694HisIle: 0.694 ± 0.022	0.299HisLys: 0.299 ± 0.015	2.237HisLeu: 2.237 ± 0.035	0.367HisMet: 0.367 ± 0.014	0.413HisAsn: 0.413 ± 0.018	1.677HisPro: 1.677 ± 0.029	0.552HisGln: 0.552 ± 0.02	1.975HisArg: 1.975 ± 0.041	1.103HisSer: 1.103 ± 0.025	1.218HisThr: 1.218 ± 0.026	1.741HisVal: 1.741 ± 0.033	0.314HisTrp: 0.314 ± 0.014	0.449HisTyr: 0.449 ± 0.018	0.001HisXaa: 0.001 ± 0.001
Ile	6.008IleAla: 6.008 ± 0.062	0.303IleCys: 0.303 ± 0.013	3.068IleAsp: 3.068 ± 0.046	2.446IleGlu: 2.446 ± 0.039	0.868IlePhe: 0.868 ± 0.024	4.524IleGly: 4.524 ± 0.058	0.677IleHis: 0.677 ± 0.018	1.061IleIle: 1.061 ± 0.029	0.626IleLys: 0.626 ± 0.021	3.131IleLeu: 3.131 ± 0.052	0.533IleMet: 0.533 ± 0.016	0.759IleAsn: 0.759 ± 0.025	2.194IlePro: 2.194 ± 0.039	0.739IleGln: 0.739 ± 0.02	2.602IleArg: 2.602 ± 0.045	1.977IleSer: 1.977 ± 0.037	2.5IleThr: 2.5 ± 0.037	3.811IleVal: 3.811 ± 0.046	0.386IleTrp: 0.386 ± 0.016	0.678IleTyr: 0.678 ± 0.021	0.0IleXaa: 0.0 ± 0.0
Lys	2.391LysAla: 2.391 ± 0.054	0.111LysCys: 0.111 ± 0.009	1.021LysAsp: 1.021 ± 0.025	0.882LysGlu: 0.882 ± 0.024	0.565LysPhe: 0.565 ± 0.017	1.41LysGly: 1.41 ± 0.032	0.408LysHis: 0.408 ± 0.017	0.882LysIle: 0.882 ± 0.025	0.587LysLys: 0.587 ± 0.025	1.845LysLeu: 1.845 ± 0.033	0.411LysMet: 0.411 ± 0.018	0.462LysAsn: 0.462 ± 0.018	1.16LysPro: 1.16 ± 0.029	0.628LysGln: 0.628 ± 0.02	1.332LysArg: 1.332 ± 0.034	1.156LysSer: 1.156 ± 0.031	1.107LysThr: 1.107 ± 0.025	1.787LysVal: 1.787 ± 0.034	0.269LysTrp: 0.269 ± 0.012	0.438LysTyr: 0.438 ± 0.017	0.001LysXaa: 0.001 ± 0.001
Leu	14.046LeuAla: 14.046 ± 0.112	0.792LeuCys: 0.792 ± 0.022	7.19LeuAsp: 7.19 ± 0.089	4.451LeuGlu: 4.451 ± 0.055	2.684LeuPhe: 2.684 ± 0.046	9.323LeuGly: 9.323 ± 0.088	2.05LeuHis: 2.05 ± 0.035	3.621LeuIle: 3.621 ± 0.054	1.432LeuLys: 1.432 ± 0.032	9.535LeuLeu: 9.535 ± 0.092	1.634LeuMet: 1.634 ± 0.031	1.692LeuAsn: 1.692 ± 0.028	5.8LeuPro: 5.8 ± 0.068	2.219LeuGln: 2.219 ± 0.04	7.65LeuArg: 7.65 ± 0.068	5.393LeuSer: 5.393 ± 0.057	6.78LeuThr: 6.78 ± 0.064	9.287LeuVal: 9.287 ± 0.085	1.157LeuTrp: 1.157 ± 0.032	1.586LeuTyr: 1.586 ± 0.032	0.001LeuXaa: 0.001 ± 0.001
Met	2.264MetAla: 2.264 ± 0.04	0.186MetCys: 0.186 ± 0.012	0.88MetAsp: 0.88 ± 0.025	0.657MetGlu: 0.657 ± 0.023	0.59MetPhe: 0.59 ± 0.019	1.426MetGly: 1.426 ± 0.029	0.427MetHis: 0.427 ± 0.016	0.899MetIle: 0.899 ± 0.021	0.423MetLys: 0.423 ± 0.016	1.955MetLeu: 1.955 ± 0.035	0.4MetMet: 0.4 ± 0.017	0.491MetAsn: 0.491 ± 0.019	1.146MetPro: 1.146 ± 0.028	0.529MetGln: 0.529 ± 0.016	1.558MetArg: 1.558 ± 0.033	1.598MetSer: 1.598 ± 0.032	1.841MetThr: 1.841 ± 0.038	1.63MetVal: 1.63 ± 0.036	0.274MetTrp: 0.274 ± 0.014	0.351MetTyr: 0.351 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	2.418AsnAla: 2.418 ± 0.04	0.163AsnCys: 0.163 ± 0.01	1.006AsnAsp: 1.006 ± 0.026	0.935AsnGlu: 0.935 ± 0.026	0.576AsnPhe: 0.576 ± 0.017	1.907AsnGly: 1.907 ± 0.035	0.45AsnHis: 0.45 ± 0.017	0.765AsnIle: 0.765 ± 0.022	0.389AsnLys: 0.389 ± 0.016	2.021AsnLeu: 2.021 ± 0.037	0.378AsnMet: 0.378 ± 0.015	0.471AsnAsn: 0.471 ± 0.02	1.681AsnPro: 1.681 ± 0.029	0.525AsnGln: 0.525 ± 0.018	1.414AsnArg: 1.414 ± 0.029	1.148AsnSer: 1.148 ± 0.03	1.275AsnThr: 1.275 ± 0.029	1.603AsnVal: 1.603 ± 0.035	0.33AsnTrp: 0.33 ± 0.013	0.45AsnTyr: 0.45 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	7.244ProAla: 7.244 ± 0.077	0.279ProCys: 0.279 ± 0.015	4.706ProAsp: 4.706 ± 0.06	3.766ProGlu: 3.766 ± 0.048	1.539ProPhe: 1.539 ± 0.03	5.623ProGly: 5.623 ± 0.064	1.102ProHis: 1.102 ± 0.024	2.027ProIle: 2.027 ± 0.041	1.13ProLys: 1.13 ± 0.026	4.891ProLeu: 4.891 ± 0.062	1.094ProMet: 1.094 ± 0.023	1.219ProAsn: 1.219 ± 0.031	2.841ProPro: 2.841 ± 0.053	1.598ProGln: 1.598 ± 0.039	3.658ProArg: 3.658 ± 0.056	3.176ProSer: 3.176 ± 0.048	3.783ProThr: 3.783 ± 0.059	5.348ProVal: 5.348 ± 0.061	0.773ProTrp: 0.773 ± 0.023	1.038ProTyr: 1.038 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.172GlnAla: 3.172 ± 0.045	0.198GlnCys: 0.198 ± 0.011	1.201GlnAsp: 1.201 ± 0.024	1.125GlnGlu: 1.125 ± 0.028	0.889GlnPhe: 0.889 ± 0.023	1.874GlnGly: 1.874 ± 0.035	0.596GlnHis: 0.596 ± 0.021	1.328GlnIle: 1.328 ± 0.029	0.538GlnLys: 0.538 ± 0.019	2.945GlnLeu: 2.945 ± 0.05	0.602GlnMet: 0.602 ± 0.021	0.549GlnAsn: 0.549 ± 0.017	1.52GlnPro: 1.52 ± 0.035	1.146GlnGln: 1.146 ± 0.032	2.336GlnArg: 2.336 ± 0.047	1.47GlnSer: 1.47 ± 0.031	1.321GlnThr: 1.321 ± 0.025	2.698GlnVal: 2.698 ± 0.049	0.537GlnTrp: 0.537 ± 0.019	0.59GlnTyr: 0.59 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	8.634ArgAla: 8.634 ± 0.074	0.607ArgCys: 0.607 ± 0.019	4.535ArgAsp: 4.535 ± 0.052	4.101ArgGlu: 4.101 ± 0.047	2.389ArgPhe: 2.389 ± 0.038	5.742ArgGly: 5.742 ± 0.058	1.786ArgHis: 1.786 ± 0.035	3.551ArgIle: 3.551 ± 0.046	1.457ArgLys: 1.457 ± 0.032	7.144ArgLeu: 7.144 ± 0.082	1.913ArgMet: 1.913 ± 0.033	1.493ArgAsn: 1.493 ± 0.03	4.106ArgPro: 4.106 ± 0.049	2.044ArgGln: 2.044 ± 0.036	6.945ArgArg: 6.945 ± 0.097	4.476ArgSer: 4.476 ± 0.055	4.856ArgThr: 4.856 ± 0.054	6.105ArgVal: 6.105 ± 0.065	1.339ArgTrp: 1.339 ± 0.028	1.76ArgTyr: 1.76 ± 0.033	0.001ArgXaa: 0.001 ± 0.001
Ser	7.124SerAla: 7.124 ± 0.075	0.412SerCys: 0.412 ± 0.015	3.446SerAsp: 3.446 ± 0.051	2.763SerGlu: 2.763 ± 0.048	1.6SerPhe: 1.6 ± 0.035	6.23SerGly: 6.23 ± 0.077	0.989SerHis: 0.989 ± 0.023	2.181SerIle: 2.181 ± 0.034	1.038SerLys: 1.038 ± 0.027	4.956SerLeu: 4.956 ± 0.059	1.376SerMet: 1.376 ± 0.031	1.07SerAsn: 1.07 ± 0.024	3.035SerPro: 3.035 ± 0.042	1.307SerGln: 1.307 ± 0.031	3.946SerArg: 3.946 ± 0.056	3.208SerSer: 3.208 ± 0.063	3.782SerThr: 3.782 ± 0.051	5.028SerVal: 5.028 ± 0.054	0.917SerTrp: 0.917 ± 0.025	1.102SerTyr: 1.102 ± 0.023	0.001SerXaa: 0.001 ± 0.001
Thr	8.493ThrAla: 8.493 ± 0.085	0.442ThrCys: 0.442 ± 0.017	4.228ThrAsp: 4.228 ± 0.054	3.496ThrGlu: 3.496 ± 0.053	1.825ThrPhe: 1.825 ± 0.033	6.262ThrGly: 6.262 ± 0.062	1.202ThrHis: 1.202 ± 0.03	2.402ThrIle: 2.402 ± 0.037	1.138ThrLys: 1.138 ± 0.03	5.976ThrLeu: 5.976 ± 0.07	1.201ThrMet: 1.201 ± 0.026	1.19ThrAsn: 1.19 ± 0.034	4.052ThrPro: 4.052 ± 0.052	1.434ThrGln: 1.434 ± 0.028	4.089ThrArg: 4.089 ± 0.052	3.461ThrSer: 3.461 ± 0.054	4.474ThrThr: 4.474 ± 0.09	6.764ThrVal: 6.764 ± 0.075	0.895ThrTrp: 0.895 ± 0.022	1.241ThrTyr: 1.241 ± 0.027	0.001ThrXaa: 0.001 ± 0.001
Val	12.543ValAla: 12.543 ± 0.115	0.747ValCys: 0.747 ± 0.023	6.696ValAsp: 6.696 ± 0.077	4.961ValGlu: 4.961 ± 0.059	2.568ValPhe: 2.568 ± 0.044	7.78ValGly: 7.78 ± 0.09	1.994ValHis: 1.994 ± 0.033	3.428ValIle: 3.428 ± 0.046	1.521ValLys: 1.521 ± 0.032	9.475ValLeu: 9.475 ± 0.088	1.582ValMet: 1.582 ± 0.031	1.912ValAsn: 1.912 ± 0.04	5.295ValPro: 5.295 ± 0.068	2.097ValGln: 2.097 ± 0.034	6.567ValArg: 6.567 ± 0.07	4.759ValSer: 4.759 ± 0.054	6.514ValThr: 6.514 ± 0.071	9.663ValVal: 9.663 ± 0.103	1.14ValTrp: 1.14 ± 0.027	1.499ValTyr: 1.499 ± 0.026	0.002ValXaa: 0.002 ± 0.001
Trp	1.532TrpAla: 1.532 ± 0.032	0.149TrpCys: 0.149 ± 0.009	0.764TrpAsp: 0.764 ± 0.024	0.664TrpGlu: 0.664 ± 0.019	0.529TrpPhe: 0.529 ± 0.021	1.02TrpGly: 1.02 ± 0.028	0.312TrpHis: 0.312 ± 0.014	0.691TrpIle: 0.691 ± 0.022	0.296TrpLys: 0.296 ± 0.016	1.594TrpLeu: 1.594 ± 0.041	0.358TrpMet: 0.358 ± 0.013	0.426TrpAsn: 0.426 ± 0.015	0.738TrpPro: 0.738 ± 0.022	0.55TrpGln: 0.55 ± 0.016	1.151TrpArg: 1.151 ± 0.026	0.954TrpSer: 0.954 ± 0.025	0.981TrpThr: 0.981 ± 0.022	1.104TrpVal: 1.104 ± 0.026	0.34TrpTrp: 0.34 ± 0.015	0.313TrpTyr: 0.313 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.235TyrAla: 2.235 ± 0.037	0.188TyrCys: 0.188 ± 0.011	1.24TyrAsp: 1.24 ± 0.029	1.004TyrGlu: 1.004 ± 0.027	0.679TyrPhe: 0.679 ± 0.021	1.945TyrGly: 1.945 ± 0.039	0.41TyrHis: 0.41 ± 0.017	0.571TyrIle: 0.571 ± 0.019	0.312TyrLys: 0.312 ± 0.014	2.16TyrLeu: 2.16 ± 0.035	0.294TyrMet: 0.294 ± 0.014	0.402TyrAsn: 0.402 ± 0.016	1.114TyrPro: 1.114 ± 0.023	0.549TyrGln: 0.549 ± 0.017	1.742TyrArg: 1.742 ± 0.038	1.136TyrSer: 1.136 ± 0.028	1.187TyrThr: 1.187 ± 0.026	1.608TyrVal: 1.608 ± 0.035	0.358TyrTrp: 0.358 ± 0.014	0.411TyrTyr: 0.411 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.003XaaGly: 0.003 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5489 proteins (1736644 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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