Columba livia (Rock dove)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria;

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I0M3C1|A0A2I0M3C1_COLLI DNAation factor 45kDa alpha polypeptide OS=Columba livia OX=8932 GN=DFFA PE=4 SV=1
TT1 pKa = 7.34LYY3 pKa = 10.23IAEE6 pKa = 4.85SRR8 pKa = 11.84LSWLEE13 pKa = 3.52NSGVGFSLDD22 pKa = 3.46YY23 pKa = 9.24PTISLHH29 pKa = 6.16AVSRR33 pKa = 11.84DD34 pKa = 3.02LTAYY38 pKa = 9.06PWEE41 pKa = 4.27HH42 pKa = 7.2LYY44 pKa = 10.9IMVNAKK50 pKa = 10.3FEE52 pKa = 4.03EE53 pKa = 4.92AEE55 pKa = 4.28TKK57 pKa = 9.17EE58 pKa = 4.1APLAEE63 pKa = 4.48GEE65 pKa = 4.27EE66 pKa = 4.58EE67 pKa = 4.38EE68 pKa = 6.2DD69 pKa = 4.13SDD71 pKa = 6.02DD72 pKa = 3.85DD73 pKa = 4.68AEE75 pKa = 5.15PISEE79 pKa = 4.02FRR81 pKa = 11.84FVPSDD86 pKa = 3.36KK87 pKa = 10.88SALEE91 pKa = 3.78AMFAAMCEE99 pKa = 4.38CQALHH104 pKa = 7.41PDD106 pKa = 4.21PDD108 pKa = 5.77DD109 pKa = 4.05EE110 pKa = 7.61DD111 pKa = 4.46SDD113 pKa = 3.89NDD115 pKa = 3.96YY116 pKa = 11.58EE117 pKa = 4.77GDD119 pKa = 3.7EE120 pKa = 4.54YY121 pKa = 11.44DD122 pKa = 4.11VEE124 pKa = 4.28AHH126 pKa = 6.16EE127 pKa = 5.43LEE129 pKa = 4.54QGDD132 pKa = 3.73IPTFCTYY139 pKa = 11.03EE140 pKa = 3.97EE141 pKa = 4.65GLLHH145 pKa = 6.43LTAEE149 pKa = 4.65GQATLEE155 pKa = 4.11RR156 pKa = 11.84LEE158 pKa = 4.74GMLAQSVSSQYY169 pKa = 11.66NMAGVRR175 pKa = 11.84TEE177 pKa = 3.96DD178 pKa = 3.14SVRR181 pKa = 11.84EE182 pKa = 4.08YY183 pKa = 10.96EE184 pKa = 5.41DD185 pKa = 3.43GMEE188 pKa = 4.1VDD190 pKa = 3.96TAPVVAGQFEE200 pKa = 4.42DD201 pKa = 5.26AEE203 pKa = 4.32VDD205 pKa = 3.59HH206 pKa = 7.08

Molecular weight: 23.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I0LK77|A0A2I0LK77_COLLI DUF1741 domain-containing protein OS=Columba livia OX=8932 GN=A306_00014676 PE=4 SV=1
MM1 pKa = 7.43PSAWPRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84SVSTGANSRR18 pKa = 11.84PRR20 pKa = 11.84PRR22 pKa = 11.84SSRR25 pKa = 11.84PSKK28 pKa = 9.43GHH30 pKa = 6.08RR31 pKa = 11.84SHH33 pKa = 5.85WRR35 pKa = 11.84GRR37 pKa = 11.84GRR39 pKa = 11.84TRR41 pKa = 11.84RR42 pKa = 11.84SAIMQMSCPTRR53 pKa = 11.84KK54 pKa = 9.31PSSSLTVSS62 pKa = 3.3

Molecular weight: 7.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14619

2690

17309

10015450

10

20645

578.6

64.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.679 ± 0.015

2.206 ± 0.014

5.054 ± 0.01

7.293 ± 0.025

3.646 ± 0.013

6.075 ± 0.023

2.52 ± 0.008

4.659 ± 0.014

6.282 ± 0.021

9.473 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.009

4.007 ± 0.012

5.627 ± 0.022

4.756 ± 0.018

5.377 ± 0.017

8.397 ± 0.023

5.492 ± 0.016

6.171 ± 0.015

1.171 ± 0.006

2.806 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski