Flavipsychrobacter stenotrophus
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4474 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S7T141|A0A2S7T141_9BACT Uncharacterized protein OS=Flavipsychrobacter stenotrophus OX=2077091 GN=CJD36_002580 PE=4 SV=1
MM1 pKa = 7.57 FDD3 pKa = 4.25 IITSRR8 pKa = 11.84 GTDD11 pKa = 3.19 GTQFIAIEE19 pKa = 4.21 EE20 pKa = 4.59 YY21 pKa = 9.83 IPEE24 pKa = 4.18 TFVYY28 pKa = 10.09 GGNGTQGNLLDD39 pKa = 4.49 GNGNQVYY46 pKa = 8.08 ATYY49 pKa = 10.47 AGKK52 pKa = 10.66 RR53 pKa = 11.84 NISWTIMPANSVFKK67 pKa = 10.06 FVNLASTPATYY78 pKa = 10.6 EE79 pKa = 3.59 LDD81 pKa = 3.81 GNGDD85 pKa = 3.82 VLNDD89 pKa = 3.31 IDD91 pKa = 4.46 GVPVLSTDD99 pKa = 4.37 FSAEE103 pKa = 3.78 QVNVVITSSSYY114 pKa = 10.59 TFICNRR120 pKa = 11.84 DD121 pKa = 3.78 EE122 pKa = 4.56 IGVIATNVADD132 pKa = 5.18 DD133 pKa = 4.73 LDD135 pKa = 4.87 AFTLSNGLLSSLYY148 pKa = 10.35 KK149 pKa = 10.72 AA150 pKa = 4.51
Molecular weight: 16.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.77
IPC_protein 3.732
Toseland 3.516
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.681
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.859
Patrickios 0.846
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A2S7SSX6|A0A2S7SSX6_9BACT YceI domain-containing protein OS=Flavipsychrobacter stenotrophus OX=2077091 GN=CJD36_017535 PE=4 SV=1
MM1 pKa = 7.9 PKK3 pKa = 9.66 MKK5 pKa = 9.06 THH7 pKa = 5.83 SRR9 pKa = 11.84 AKK11 pKa = 8.95 KK12 pKa = 7.09 TFRR15 pKa = 11.84 VTGTGKK21 pKa = 9.5 IRR23 pKa = 11.84 RR24 pKa = 11.84 YY25 pKa = 9.91 KK26 pKa = 10.45 AFKK29 pKa = 10.52 SHH31 pKa = 7.38 LLTKK35 pKa = 10.15 KK36 pKa = 9.02 SKK38 pKa = 9.67 SRR40 pKa = 11.84 KK41 pKa = 7.14 RR42 pKa = 11.84 HH43 pKa = 4.7 LRR45 pKa = 11.84 IHH47 pKa = 6.07 GLVHH51 pKa = 6.82 KK52 pKa = 10.97 SNMPLVKK59 pKa = 10.34 RR60 pKa = 11.84 MLVMGG65 pKa = 4.76
Molecular weight: 7.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.367
IPC2_protein 11.038
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.486
Grimsley 12.559
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.193
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.933
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4474
0
4474
1561615
25
4864
349.0
38.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.884 ± 0.053
1.15 ± 0.015
5.188 ± 0.035
4.841 ± 0.06
4.433 ± 0.032
7.349 ± 0.056
1.944 ± 0.02
7.19 ± 0.038
6.114 ± 0.069
8.497 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.566 ± 0.024
5.573 ± 0.028
3.903 ± 0.028
3.2 ± 0.029
3.605 ± 0.037
6.871 ± 0.066
7.426 ± 0.115
6.834 ± 0.035
1.147 ± 0.013
4.285 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here