Candidatus Cardinium hertigii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Amoebophilaceae; Candidatus Cardinium

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3LA25|A0A2Z3LA25_9BACT Primosomal protein N' OS=Candidatus Cardinium hertigii OX=247481 GN=priA PE=3 SV=1
MM1 pKa = 7.24YY2 pKa = 7.76WTLEE6 pKa = 4.38LISYY10 pKa = 10.47LEE12 pKa = 4.26DD13 pKa = 3.98APWPATKK20 pKa = 10.54DD21 pKa = 3.51EE22 pKa = 5.26LIDD25 pKa = 3.49YY26 pKa = 8.19AKK28 pKa = 10.61RR29 pKa = 11.84SGAPLEE35 pKa = 4.54VISNLEE41 pKa = 3.78EE42 pKa = 4.84LDD44 pKa = 3.73NDD46 pKa = 4.07DD47 pKa = 4.22YY48 pKa = 11.39PYY50 pKa = 11.46SSVEE54 pKa = 4.5EE55 pKa = 4.0IWPDD59 pKa = 3.63YY60 pKa = 8.44PTGDD64 pKa = 3.16DD65 pKa = 3.85FVFNEE70 pKa = 4.67DD71 pKa = 3.35EE72 pKa = 4.34YY73 pKa = 11.97

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3L8I8|A0A2Z3L8I8_9BACT Putative chromosome-partitioning protein ParB OS=Candidatus Cardinium hertigii OX=247481 GN=parB PE=3 SV=1
MM1 pKa = 7.37GRR3 pKa = 11.84GEE5 pKa = 4.2RR6 pKa = 11.84DD7 pKa = 3.43SLKK10 pKa = 10.68SSIMAVKK17 pKa = 10.11IRR19 pKa = 11.84LAKK22 pKa = 9.95CGRR25 pKa = 11.84RR26 pKa = 11.84HH27 pKa = 4.16WAEE30 pKa = 3.54YY31 pKa = 10.05HH32 pKa = 6.44IVVADD37 pKa = 3.75ARR39 pKa = 11.84APRR42 pKa = 11.84DD43 pKa = 3.15GRR45 pKa = 11.84FIEE48 pKa = 4.98KK49 pKa = 10.01LGHH52 pKa = 5.79YY53 pKa = 9.1NPHH56 pKa = 6.11TAPSTVHH63 pKa = 6.09LNEE66 pKa = 3.41VRR68 pKa = 11.84ALKK71 pKa = 9.89WLLQGAQPTDD81 pKa = 3.24TVRR84 pKa = 11.84NLFSRR89 pKa = 11.84QGILLKK95 pKa = 10.61KK96 pKa = 9.07HH97 pKa = 5.62LQVGVGKK104 pKa = 10.31GAITQEE110 pKa = 3.95QAEE113 pKa = 4.44EE114 pKa = 5.11RR115 pKa = 11.84FTAWQAQVRR124 pKa = 11.84RR125 pKa = 4.08

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

963

0

963

321799

29

3835

334.2

37.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.424 ± 0.104

1.495 ± 0.032

4.041 ± 0.053

5.553 ± 0.079

4.237 ± 0.058

5.654 ± 0.086

2.6 ± 0.04

7.852 ± 0.076

6.936 ± 0.087

11.391 ± 0.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.038

4.11 ± 0.084

3.987 ± 0.049

4.623 ± 0.063

4.139 ± 0.054

5.901 ± 0.066

5.758 ± 0.053

5.87 ± 0.069

1.089 ± 0.025

3.96 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski