Streptococcus phage Javan131

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A1E8|A0A4D6A1E8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan131 OX=2547995 GN=Javan131_0052 PE=4 SV=1
MM1 pKa = 7.43QIEE4 pKa = 4.36FFKK7 pKa = 10.98NGKK10 pKa = 9.49SDD12 pKa = 4.0IEE14 pKa = 4.16LEE16 pKa = 4.16YY17 pKa = 11.02LKK19 pKa = 11.18NEE21 pKa = 3.94VNGFINDD28 pKa = 3.5LRR30 pKa = 11.84EE31 pKa = 4.39DD32 pKa = 4.36GIKK35 pKa = 10.83CLDD38 pKa = 3.17IQFIFDD44 pKa = 3.64EE45 pKa = 4.58DD46 pKa = 4.27EE47 pKa = 4.63IIVFVKK53 pKa = 10.89YY54 pKa = 10.37EE55 pKa = 3.93GLIDD59 pKa = 4.22DD60 pKa = 4.53

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A439|A0A4D6A439_9CAUD Uncharacterized protein OS=Streptococcus phage Javan131 OX=2547995 GN=Javan131_0053 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.48LFNFIFAKK10 pKa = 10.08PKK12 pKa = 9.99KK13 pKa = 9.93QEE15 pKa = 4.31EE16 pKa = 4.46KK17 pKa = 10.56PKK19 pKa = 8.82WTIEE23 pKa = 4.08NNGWEE28 pKa = 4.33ANARR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 9.06NQLHH38 pKa = 6.45GLPAKK43 pKa = 9.94QIWW46 pKa = 3.21

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13244

37

1307

220.7

24.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.166 ± 0.606

0.664 ± 0.119

6.954 ± 0.304

7.324 ± 0.256

3.473 ± 0.211

6.38 ± 0.293

1.412 ± 0.145

6.916 ± 0.287

9.046 ± 0.27

8.6 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.159

5.519 ± 0.243

2.899 ± 0.247

4.221 ± 0.266

4.032 ± 0.2

5.852 ± 0.305

5.897 ± 0.187

6.229 ± 0.249

1.314 ± 0.134

3.76 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski