Pontiella desulfatans

Taxonomy: cellular organisms; Bacteria; PVC group; Kiritimatiellaeota; Kiritimatiellae; Kiritimatiellales; Pontiellaceae; Pontiella

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6374 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6C2TX91|A0A6C2TX91_9BACT Signal peptidase I OS=Pontiella desulfatans OX=2750659 GN=sipV PE=3 SV=1
MM1 pKa = 7.29KK2 pKa = 10.27AKK4 pKa = 10.36AAIAGTLLALILAGSAVAQEE24 pKa = 4.37EE25 pKa = 4.52FDD27 pKa = 5.24FGDD30 pKa = 3.97APDD33 pKa = 4.6PGFPTLLANNGARR46 pKa = 11.84HH47 pKa = 5.55LVSIGSQHH55 pKa = 6.3IFLGLAPVDD64 pKa = 4.2TEE66 pKa = 4.75PDD68 pKa = 3.66GQPHH72 pKa = 6.49INAIGDD78 pKa = 4.12DD79 pKa = 3.89NDD81 pKa = 3.46IAYY84 pKa = 7.78PPPFDD89 pKa = 6.06DD90 pKa = 4.31EE91 pKa = 6.47DD92 pKa = 4.13GVSWDD97 pKa = 3.64TPLIPGQVAQITVTASVNGDD117 pKa = 3.04LDD119 pKa = 3.31AWIDD123 pKa = 3.56FSGNNDD129 pKa = 2.69WTTVGDD135 pKa = 4.44QIFTTQPLIAGANTLNFNVPVWASPGYY162 pKa = 7.95TYY164 pKa = 11.55ARR166 pKa = 11.84FRR168 pKa = 11.84FNASGVALQHH178 pKa = 6.2VGPAPDD184 pKa = 4.91GEE186 pKa = 4.42VEE188 pKa = 4.98DD189 pKa = 4.56YY190 pKa = 10.73FIQITQEE197 pKa = 4.14LEE199 pKa = 4.3DD200 pKa = 4.67FYY202 pKa = 11.62DD203 pKa = 3.52WGDD206 pKa = 3.92APDD209 pKa = 3.95SASSPKK215 pKa = 10.43YY216 pKa = 8.36PTLAANNGANHH227 pKa = 7.84LINASVYY234 pKa = 9.96LGAVVDD240 pKa = 4.53IDD242 pKa = 6.08LDD244 pKa = 4.07GQPHH248 pKa = 6.56AKK250 pKa = 10.52ALGDD254 pKa = 5.36DD255 pKa = 3.82INQAYY260 pKa = 8.71PGSPYY265 pKa = 10.42PPGDD269 pKa = 3.3EE270 pKa = 4.96DD271 pKa = 5.5GIAFTSLLVQNQTATLTATASTAGYY296 pKa = 9.56LDD298 pKa = 3.27AWIDD302 pKa = 3.79YY303 pKa = 9.72NQDD306 pKa = 3.53GVWNAAEE313 pKa = 5.04KK314 pKa = 10.41IFSSTPLGAGANALSVTIPGGATAGKK340 pKa = 7.53TFARR344 pKa = 11.84FRR346 pKa = 11.84FSLNGGLAPNGTAPDD361 pKa = 4.29GEE363 pKa = 4.46VEE365 pKa = 4.98DD366 pKa = 4.25YY367 pKa = 10.72EE368 pKa = 4.52VWIEE372 pKa = 4.13TTEE375 pKa = 4.2EE376 pKa = 3.81DD377 pKa = 5.67HH378 pKa = 7.43KK379 pKa = 10.45MHH381 pKa = 6.91WPQYY385 pKa = 8.11PDD387 pKa = 3.08PHH389 pKa = 7.62GWDD392 pKa = 3.17VRR394 pKa = 11.84ASYY397 pKa = 11.45LDD399 pKa = 4.08GNPDD403 pKa = 3.56DD404 pKa = 4.77QFHH407 pKa = 6.84KK408 pKa = 11.09VLADD412 pKa = 4.41DD413 pKa = 5.61FLCTSNGPITKK424 pKa = 8.51ITFWGSFQYY433 pKa = 10.78NGYY436 pKa = 9.96IPTNFHH442 pKa = 6.5KK443 pKa = 10.87FNGISKK449 pKa = 8.89FHH451 pKa = 6.51FSFHH455 pKa = 6.78KK456 pKa = 10.29DD457 pKa = 3.03IPVDD461 pKa = 3.89LPEE464 pKa = 5.4IPYY467 pKa = 10.21SRR469 pKa = 11.84PVVPAEE475 pKa = 3.74WEE477 pKa = 3.82IDD479 pKa = 3.26VDD481 pKa = 4.59PFMLPAGWEE490 pKa = 3.91ITVTTEE496 pKa = 3.58EE497 pKa = 4.76PSWQGWYY504 pKa = 10.25DD505 pKa = 3.94PNTGNWNTNDD515 pKa = 3.11HH516 pKa = 5.31NHH518 pKa = 5.52YY519 pKa = 9.92FRR521 pKa = 11.84YY522 pKa = 10.42DD523 pKa = 3.2ITIPEE528 pKa = 4.28ADD530 pKa = 3.5AFVQVEE536 pKa = 4.4SNIYY540 pKa = 8.89WLDD543 pKa = 3.08ISVDD547 pKa = 3.77LEE549 pKa = 4.12GTEE552 pKa = 6.05GYY554 pKa = 9.15WGWKK558 pKa = 6.85TSRR561 pKa = 11.84SPHH564 pKa = 5.87WNDD567 pKa = 3.1DD568 pKa = 3.97AVWADD573 pKa = 3.86LPVEE577 pKa = 4.59FHH579 pKa = 5.49QQWGEE584 pKa = 4.11LIDD587 pKa = 4.82PIYY590 pKa = 11.19SNSLDD595 pKa = 3.52LAFIINDD602 pKa = 3.34EE603 pKa = 4.31VEE605 pKa = 4.15PEE607 pKa = 3.89EE608 pKa = 4.36TYY610 pKa = 11.3DD611 pKa = 3.56WGDD614 pKa = 4.23APDD617 pKa = 3.7QPYY620 pKa = 7.72PTLAASSGANHH631 pKa = 7.34LIVPGVFMGTLIDD644 pKa = 5.19AEE646 pKa = 4.44TDD648 pKa = 3.53GQPNATATGDD658 pKa = 3.96DD659 pKa = 4.0LAGPAADD666 pKa = 5.3DD667 pKa = 3.55EE668 pKa = 5.24DD669 pKa = 4.24GVVFNTPLFPGQPALIDD686 pKa = 3.6VTVSQAGWLDD696 pKa = 3.14VWLDD700 pKa = 3.34ADD702 pKa = 5.57NNGDD706 pKa = 3.36WLGPNDD712 pKa = 4.57LVYY715 pKa = 10.58SAYY718 pKa = 10.65VSAGLNPLSINIPLSVPPGQNFMRR742 pKa = 11.84FRR744 pKa = 11.84YY745 pKa = 7.45NTNGPLPPTGGAQNGEE761 pKa = 4.18VEE763 pKa = 4.7DD764 pKa = 4.34YY765 pKa = 10.92EE766 pKa = 4.6VDD768 pKa = 3.44IEE770 pKa = 5.4LEE772 pKa = 4.2PEE774 pKa = 4.31LDD776 pKa = 4.62FGDD779 pKa = 4.79APDD782 pKa = 4.55PGYY785 pKa = 8.02PTWLAMNGARR795 pKa = 11.84HH796 pKa = 6.63IIYY799 pKa = 9.96GGPAIALRR807 pKa = 11.84MGAKK811 pKa = 9.66VDD813 pKa = 4.28ADD815 pKa = 3.75PDD817 pKa = 4.11GQPTAASDD825 pKa = 4.03GDD827 pKa = 4.04DD828 pKa = 3.61TDD830 pKa = 5.04GSDD833 pKa = 3.88DD834 pKa = 3.74EE835 pKa = 4.4EE836 pKa = 5.87SIVFTTPIIPGTWAKK851 pKa = 10.01MDD853 pKa = 3.55LTVGVGIPASGYY865 pKa = 10.55AWLQGWADD873 pKa = 3.96FNNDD877 pKa = 4.03GDD879 pKa = 3.91WLDD882 pKa = 3.73AGEE885 pKa = 4.76QIFADD890 pKa = 3.71EE891 pKa = 4.72LVTFGASGSGVNNLSFPVPTTALPGALHH919 pKa = 6.25LRR921 pKa = 11.84LRR923 pKa = 11.84LSTYY927 pKa = 10.2KK928 pKa = 10.93GIGITGLAYY937 pKa = 10.4DD938 pKa = 4.95GEE940 pKa = 4.58VEE942 pKa = 4.44DD943 pKa = 4.36YY944 pKa = 10.34LVHH947 pKa = 7.78VEE949 pKa = 4.33EE950 pKa = 5.41PMDD953 pKa = 4.51IDD955 pKa = 3.84WGDD958 pKa = 4.14AFDD961 pKa = 3.89NWITPVYY968 pKa = 8.71PTILLNNGAHH978 pKa = 5.78HH979 pKa = 6.81VIDD982 pKa = 4.33GVHH985 pKa = 6.16FLGGQIDD992 pKa = 4.16AEE994 pKa = 4.34ADD996 pKa = 3.67GQPAIAVDD1004 pKa = 3.84GDD1006 pKa = 4.73DD1007 pKa = 4.22IVGPSPDD1014 pKa = 4.55DD1015 pKa = 3.44EE1016 pKa = 6.74DD1017 pKa = 5.06GVFFTTMIFAGGYY1030 pKa = 9.45VGVDD1034 pKa = 2.73VDD1036 pKa = 3.7ASTNGVLDD1044 pKa = 3.27AWVDD1048 pKa = 4.04FNNDD1052 pKa = 3.25GDD1054 pKa = 4.02WDD1056 pKa = 4.19DD1057 pKa = 5.15LNEE1060 pKa = 4.8QIFSGQNLHH1069 pKa = 6.97PGINSLGFTVPASAATVYY1087 pKa = 10.48PITSRR1092 pKa = 11.84FRR1094 pKa = 11.84FTSGGISTYY1103 pKa = 10.37TGLAMDD1109 pKa = 5.4GEE1111 pKa = 4.87VEE1113 pKa = 4.35DD1114 pKa = 4.59HH1115 pKa = 7.05ALDD1118 pKa = 3.57IQAVDD1123 pKa = 3.5TNGLDD1128 pKa = 4.33FGDD1131 pKa = 3.96ADD1133 pKa = 3.75IFPEE1137 pKa = 4.38TVLPNGARR1145 pKa = 11.84HH1146 pKa = 6.55AIVPGIVLGSAIDD1159 pKa = 3.81WEE1161 pKa = 4.67PDD1163 pKa = 3.58GQPSAADD1170 pKa = 3.43MDD1172 pKa = 4.56DD1173 pKa = 3.68MNGLDD1178 pKa = 4.88DD1179 pKa = 4.15EE1180 pKa = 5.47DD1181 pKa = 4.2GVVFNNLLIAGSSGSIDD1198 pKa = 3.53VMAGSAGGQLDD1209 pKa = 3.09AWIDD1213 pKa = 3.63YY1214 pKa = 11.13GNDD1217 pKa = 2.9FSFVGDD1223 pKa = 4.21HH1224 pKa = 6.01LWGGTSHH1231 pKa = 7.64PLLPGLNAGLTFTIPAPPLAIGPVFARR1258 pKa = 11.84FRR1260 pKa = 11.84ISSGGGLAPTGLATDD1275 pKa = 4.89GEE1277 pKa = 4.67VEE1279 pKa = 4.43DD1280 pKa = 4.43YY1281 pKa = 10.79LVEE1284 pKa = 4.45LFQPAPTVPLKK1295 pKa = 9.77ITNLTFNASCTTSTVEE1311 pKa = 3.62WTVQSGITYY1320 pKa = 9.69EE1321 pKa = 4.62LEE1323 pKa = 3.95STTNLVTGPWMYY1335 pKa = 11.09VPGNPVIGPANSLTDD1350 pKa = 3.39NMSAEE1355 pKa = 3.97TSKK1358 pKa = 10.63FYY1360 pKa = 10.52RR1361 pKa = 11.84VRR1363 pKa = 11.84APYY1366 pKa = 9.59TPP1368 pKa = 3.8

Molecular weight: 146.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6C2U951|A0A6C2U951_9BACT Uncharacterized protein OS=Pontiella desulfatans OX=2750659 GN=PDESU_04503 PE=4 SV=1
MM1 pKa = 6.56TALRR5 pKa = 11.84EE6 pKa = 4.05RR7 pKa = 11.84TIQTLRR13 pKa = 11.84LGHH16 pKa = 5.56YY17 pKa = 8.12SEE19 pKa = 5.49RR20 pKa = 11.84AIKK23 pKa = 9.6TYY25 pKa = 10.73INGLARR31 pKa = 11.84LANTTATIARR41 pKa = 11.84NLPPSASAAAPPEE54 pKa = 4.22PTGPAPWKK62 pKa = 10.3EE63 pKa = 3.7MLIISWRR70 pKa = 11.84RR71 pKa = 11.84GLALHH76 pKa = 7.12TIPIDD81 pKa = 3.84RR82 pKa = 11.84PDD84 pKa = 3.43VPGWLSPTSRR94 pKa = 11.84WHH96 pKa = 6.7LLGALISSCSLTCFSRR112 pKa = 11.84EE113 pKa = 4.13DD114 pKa = 3.24VWGSRR119 pKa = 11.84RR120 pKa = 11.84DD121 pKa = 3.28KK122 pKa = 11.01ILIVGIAA129 pKa = 3.28

Molecular weight: 14.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6374

0

6374

2580498

29

6456

404.8

44.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.961 ± 0.028

1.173 ± 0.011

6.118 ± 0.023

6.394 ± 0.032

4.116 ± 0.017

8.512 ± 0.039

2.236 ± 0.015

5.257 ± 0.02

4.869 ± 0.039

8.948 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.014

4.163 ± 0.025

4.76 ± 0.022

3.222 ± 0.015

5.156 ± 0.031

6.02 ± 0.034

5.69 ± 0.032

6.844 ± 0.024

1.735 ± 0.013

3.29 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski