Clostridium senegalense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0H6H9|A0A6M0H6H9_9CLOT Recombination protein RecR OS=Clostridium senegalense OX=1465809 GN=recR PE=3 SV=1
MM1 pKa = 7.29KK2 pKa = 10.44AVVDD6 pKa = 3.85KK7 pKa = 9.05DD8 pKa = 3.71TCISCGLCIATCPSVFDD25 pKa = 4.49FGSDD29 pKa = 3.24GKK31 pKa = 10.92SEE33 pKa = 4.81SIVDD37 pKa = 3.97SVPEE41 pKa = 4.05DD42 pKa = 3.92SIDD45 pKa = 3.62AAVEE49 pKa = 3.91ARR51 pKa = 11.84DD52 pKa = 3.95GCPVSAISLNEE63 pKa = 3.64

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0H466|A0A6M0H466_9CLOT Nitroreductase family protein OS=Clostridium senegalense OX=1465809 GN=G3M99_10775 PE=4 SV=1
MM1 pKa = 7.46FMTYY5 pKa = 8.42QPKK8 pKa = 9.63KK9 pKa = 7.83RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 8.75TKK25 pKa = 10.24SGRR28 pKa = 11.84NVLKK32 pKa = 10.48RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 9.04RR41 pKa = 11.84LTAA44 pKa = 4.18

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3405

0

3405

1045211

23

2856

307.0

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.094 ± 0.042

1.31 ± 0.018

5.426 ± 0.032

7.54 ± 0.046

4.508 ± 0.033

6.185 ± 0.042

1.276 ± 0.014

10.431 ± 0.054

9.658 ± 0.047

9.115 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.021

6.86 ± 0.042

2.619 ± 0.025

2.115 ± 0.017

3.024 ± 0.028

6.26 ± 0.032

4.805 ± 0.03

6.396 ± 0.039

0.626 ± 0.013

4.152 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski