Eubacterium callanderi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4506 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3GHY1|E3GHY1_9FIRM Nudix hydrolase domain-containing protein OS=Eubacterium callanderi OX=53442 GN=ELI_0246 PE=3 SV=1
MM1 pKa = 7.37KK2 pKa = 10.0KK3 pKa = 9.57QWIALLTGCVLVCSMLAGCGSKK25 pKa = 10.15PQTSAPAAAGSDD37 pKa = 3.46KK38 pKa = 11.26GCTDD42 pKa = 3.64EE43 pKa = 6.79AILSQLKK50 pKa = 10.53NDD52 pKa = 3.86GTPEE56 pKa = 3.84FVLDD60 pKa = 3.5GTYY63 pKa = 8.91YY64 pKa = 10.19TLPLEE69 pKa = 4.46TSQLIQAGWSLEE81 pKa = 4.04TEE83 pKa = 4.43EE84 pKa = 4.36YY85 pKa = 10.74DD86 pKa = 3.45AAEE89 pKa = 4.39VSLQPGEE96 pKa = 4.34RR97 pKa = 11.84IYY99 pKa = 11.6GEE101 pKa = 4.12LSKK104 pKa = 10.92DD105 pKa = 3.48DD106 pKa = 3.85QEE108 pKa = 5.09IDD110 pKa = 3.68VAIVNAGTEE119 pKa = 4.0PCKK122 pKa = 9.75PAEE125 pKa = 4.47GGTVVEE131 pKa = 4.99LEE133 pKa = 3.97YY134 pKa = 11.12SADD137 pKa = 3.63KK138 pKa = 10.79DD139 pKa = 3.75QPNPDD144 pKa = 3.41FFVTLNGINCAMSNAALQKK163 pKa = 10.72ALEE166 pKa = 4.44GVDD169 pKa = 4.04GYY171 pKa = 11.68KK172 pKa = 10.66LNSAGNIDD180 pKa = 3.63INRR183 pKa = 11.84TVDD186 pKa = 3.39DD187 pKa = 5.14DD188 pKa = 4.68EE189 pKa = 4.99IAVYY193 pKa = 10.17KK194 pKa = 10.76VILDD198 pKa = 4.03DD199 pKa = 5.59DD200 pKa = 4.12YY201 pKa = 11.97TAITLASDD209 pKa = 3.49NVFEE213 pKa = 4.48YY214 pKa = 10.83KK215 pKa = 10.25DD216 pKa = 3.74YY217 pKa = 11.19QPQEE221 pKa = 4.22VKK223 pKa = 10.55EE224 pKa = 3.91QASNEE229 pKa = 3.93KK230 pKa = 9.69IAAYY234 pKa = 9.89KK235 pKa = 9.47DD236 pKa = 3.29TTKK239 pKa = 11.44AEE241 pKa = 4.16MEE243 pKa = 4.73PYY245 pKa = 10.44AQDD248 pKa = 3.16FNAIIEE254 pKa = 4.69GYY256 pKa = 8.71EE257 pKa = 3.8EE258 pKa = 4.19NMVVGFYY265 pKa = 10.91SEE267 pKa = 4.06GTIWGEE273 pKa = 3.63EE274 pKa = 3.69VGEE277 pKa = 4.18YY278 pKa = 10.56KK279 pKa = 10.76SIAGTPLASDD289 pKa = 3.68VSLYY293 pKa = 10.25IAEE296 pKa = 4.48DD297 pKa = 3.59TTGQLYY303 pKa = 10.8CIANQILNEE312 pKa = 4.73DD313 pKa = 4.14GTVSTAIEE321 pKa = 4.4LEE323 pKa = 4.15EE324 pKa = 5.22GDD326 pKa = 3.88QVKK329 pKa = 10.02VWGYY333 pKa = 9.77ASQYY337 pKa = 11.32LEE339 pKa = 4.15LQEE342 pKa = 4.02GLKK345 pKa = 10.18IVVVQPGIIEE355 pKa = 4.27RR356 pKa = 11.84NGEE359 pKa = 4.17LVILDD364 pKa = 4.31KK365 pKa = 11.13NLKK368 pKa = 10.16ADD370 pKa = 3.4

Molecular weight: 40.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3GHQ4|E3GHQ4_9FIRM DNA replication and repair protein RecF OS=Eubacterium callanderi OX=53442 GN=recF PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPKK8 pKa = 8.2VRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.76KK14 pKa = 8.26EE15 pKa = 3.53HH16 pKa = 6.07GFRR19 pKa = 11.84KK20 pKa = 9.98RR21 pKa = 11.84MSTTSGRR28 pKa = 11.84VILKK32 pKa = 9.77KK33 pKa = 10.22RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.91GRR39 pKa = 11.84AKK41 pKa = 10.73LSAA44 pKa = 3.92

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4506

0

4506

1283993

14

5266

285.0

31.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.052 ± 0.042

1.422 ± 0.017

5.686 ± 0.036

7.112 ± 0.043

4.34 ± 0.031

7.308 ± 0.033

1.657 ± 0.015

7.292 ± 0.04

6.554 ± 0.032

9.284 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.981 ± 0.022

4.305 ± 0.032

3.72 ± 0.025

3.14 ± 0.02

4.365 ± 0.033

5.655 ± 0.032

5.589 ± 0.041

6.908 ± 0.031

0.861 ± 0.012

3.768 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski