Clostridium sp. BL8

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4752 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0PFT4|T0PFT4_9CLOT Uncharacterized protein OS=Clostridium sp. BL8 OX=1354301 GN=M918_13380 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 4.22MCIFQNTKK10 pKa = 9.86SCNNCGEE17 pKa = 4.32CDD19 pKa = 3.44VCEE22 pKa = 4.06LNKK25 pKa = 10.73NKK27 pKa = 9.25TCDD30 pKa = 3.28NCGKK34 pKa = 10.09CLQLEE39 pKa = 4.88GYY41 pKa = 8.82DD42 pKa = 3.54VKK44 pKa = 10.9AIKK47 pKa = 9.67IDD49 pKa = 3.68EE50 pKa = 4.3VFEE53 pKa = 4.2KK54 pKa = 11.05QEE56 pKa = 3.96DD57 pKa = 3.82SQANVSINIEE67 pKa = 4.18DD68 pKa = 4.68FSDD71 pKa = 3.59FDD73 pKa = 5.39NDD75 pKa = 4.83FEE77 pKa = 7.51DD78 pKa = 5.26DD79 pKa = 4.93DD80 pKa = 4.71SLEE83 pKa = 4.17EE84 pKa = 4.48CVDD87 pKa = 3.89DD88 pKa = 4.92YY89 pKa = 11.7IEE91 pKa = 4.47EE92 pKa = 4.36EE93 pKa = 4.53YY94 pKa = 10.59IDD96 pKa = 5.59ALDD99 pKa = 4.59EE100 pKa = 4.71ANNWQYY106 pKa = 11.23IDD108 pKa = 6.04DD109 pKa = 3.96IDD111 pKa = 3.91EE112 pKa = 4.71LKK114 pKa = 11.04DD115 pKa = 3.61LLEE118 pKa = 5.56DD119 pKa = 3.86SDD121 pKa = 4.26SLKK124 pKa = 11.19DD125 pKa = 4.03LGLEE129 pKa = 4.23KK130 pKa = 11.08YY131 pKa = 9.93PGLIVVNPNKK141 pKa = 10.19IKK143 pKa = 10.89

Molecular weight: 16.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0N0C7|T0N0C7_9CLOT Uncharacterized protein OS=Clostridium sp. BL8 OX=1354301 GN=M918_24680 PE=4 SV=1
MM1 pKa = 7.63AFDD4 pKa = 4.42RR5 pKa = 11.84NRR7 pKa = 11.84GGNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84PKK15 pKa = 10.35RR16 pKa = 11.84KK17 pKa = 9.17VCSFCIDD24 pKa = 2.89KK25 pKa = 9.65ATAIDD30 pKa = 3.63YY31 pKa = 11.22KK32 pKa = 11.28DD33 pKa = 2.98IFKK36 pKa = 10.64LKK38 pKa = 10.4KK39 pKa = 9.91YY40 pKa = 8.24VTEE43 pKa = 4.31KK44 pKa = 11.14GKK46 pKa = 10.12ILPRR50 pKa = 11.84RR51 pKa = 11.84ISGTCAKK58 pKa = 8.73HH59 pKa = 5.86QRR61 pKa = 11.84MLTVAIKK68 pKa = 10.13RR69 pKa = 11.84ARR71 pKa = 11.84NIALLPFTTEE81 pKa = 3.59

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4752

0

4752

1254797

29

2035

264.1

29.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.668 ± 0.038

1.171 ± 0.015

5.098 ± 0.027

7.78 ± 0.04

4.523 ± 0.03

6.477 ± 0.04

1.483 ± 0.015

9.42 ± 0.037

8.698 ± 0.035

9.251 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.658 ± 0.017

6.023 ± 0.03

2.839 ± 0.022

2.461 ± 0.018

3.477 ± 0.027

6.352 ± 0.031

5.052 ± 0.031

6.646 ± 0.031

0.728 ± 0.012

4.191 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski