Amino acid dipepetide frequency for Clostridium sp. BL8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.066AlaAla: 4.066 ± 0.084	0.638AlaCys: 0.638 ± 0.025	2.296AlaAsp: 2.296 ± 0.051	3.554AlaGlu: 3.554 ± 0.061	2.679AlaPhe: 2.679 ± 0.052	3.599AlaGly: 3.599 ± 0.067	0.856AlaHis: 0.856 ± 0.025	5.913AlaIle: 5.913 ± 0.076	4.491AlaLys: 4.491 ± 0.071	6.131AlaLeu: 6.131 ± 0.078	1.845AlaMet: 1.845 ± 0.049	2.546AlaAsn: 2.546 ± 0.051	1.47AlaPro: 1.47 ± 0.037	1.528AlaGln: 1.528 ± 0.036	1.906AlaArg: 1.906 ± 0.039	3.374AlaSer: 3.374 ± 0.065	3.04AlaThr: 3.04 ± 0.062	4.186AlaVal: 4.186 ± 0.058	0.347AlaTrp: 0.347 ± 0.019	2.213AlaTyr: 2.213 ± 0.041	0.002AlaXaa: 0.002 ± 0.001
Cys	0.559CysAla: 0.559 ± 0.019	0.211CysCys: 0.211 ± 0.016	0.701CysAsp: 0.701 ± 0.024	0.861CysGlu: 0.861 ± 0.026	0.522CysPhe: 0.522 ± 0.021	1.089CysGly: 1.089 ± 0.036	0.235CysHis: 0.235 ± 0.012	1.023CysIle: 1.023 ± 0.028	0.972CysLys: 0.972 ± 0.032	0.869CysLeu: 0.869 ± 0.027	0.258CysMet: 0.258 ± 0.014	0.726CysAsn: 0.726 ± 0.026	0.48CysPro: 0.48 ± 0.02	0.218CysGln: 0.218 ± 0.015	0.404CysArg: 0.404 ± 0.015	0.83CysSer: 0.83 ± 0.024	0.584CysThr: 0.584 ± 0.021	0.603CysVal: 0.603 ± 0.023	0.084CysTrp: 0.084 ± 0.008	0.485CysTyr: 0.485 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	2.385AspAla: 2.385 ± 0.055	0.542AspCys: 0.542 ± 0.023	2.135AspAsp: 2.135 ± 0.047	4.255AspGlu: 4.255 ± 0.066	2.781AspPhe: 2.781 ± 0.041	3.038AspGly: 3.038 ± 0.057	0.592AspHis: 0.592 ± 0.024	5.509AspIle: 5.509 ± 0.068	5.073AspLys: 5.073 ± 0.065	4.745AspLeu: 4.745 ± 0.066	1.489AspMet: 1.489 ± 0.036	3.133AspAsn: 3.133 ± 0.058	1.309AspPro: 1.309 ± 0.038	0.733AspGln: 0.733 ± 0.026	1.718AspArg: 1.718 ± 0.045	2.989AspSer: 2.989 ± 0.05	2.426AspThr: 2.426 ± 0.044	3.093AspVal: 3.093 ± 0.057	0.351AspTrp: 0.351 ± 0.019	2.685AspTyr: 2.685 ± 0.044	0.001AspXaa: 0.001 ± 0.001
Glu	4.826GluAla: 4.826 ± 0.073	0.632GluCys: 0.632 ± 0.023	4.559GluAsp: 4.559 ± 0.064	8.12GluGlu: 8.12 ± 0.112	3.232GluPhe: 3.232 ± 0.047	4.556GluGly: 4.556 ± 0.066	1.015GluHis: 1.015 ± 0.029	6.925GluIle: 6.925 ± 0.094	7.596GluLys: 7.596 ± 0.084	6.992GluLeu: 6.992 ± 0.079	1.885GluMet: 1.885 ± 0.039	5.172GluAsn: 5.172 ± 0.078	1.57GluPro: 1.57 ± 0.035	1.916GluGln: 1.916 ± 0.048	2.791GluArg: 2.791 ± 0.058	3.632GluSer: 3.632 ± 0.055	3.244GluThr: 3.244 ± 0.05	5.713GluVal: 5.713 ± 0.072	0.421GluTrp: 0.421 ± 0.019	3.004GluTyr: 3.004 ± 0.053	0.001GluXaa: 0.001 ± 0.001
Phe	2.334PheAla: 2.334 ± 0.046	0.544PheCys: 0.544 ± 0.024	2.283PheAsp: 2.283 ± 0.046	2.855PheGlu: 2.855 ± 0.053	2.002PhePhe: 2.002 ± 0.05	2.784PheGly: 2.784 ± 0.052	0.71PheHis: 0.71 ± 0.028	4.625PheIle: 4.625 ± 0.065	3.914PheLys: 3.914 ± 0.058	4.209PheLeu: 4.209 ± 0.07	1.245PheMet: 1.245 ± 0.031	2.985PheAsn: 2.985 ± 0.052	1.248PhePro: 1.248 ± 0.032	1.197PheGln: 1.197 ± 0.034	1.338PheArg: 1.338 ± 0.036	3.411PheSer: 3.411 ± 0.067	2.579PheThr: 2.579 ± 0.05	2.64PheVal: 2.64 ± 0.049	0.351PheTrp: 0.351 ± 0.02	1.974PheTyr: 1.974 ± 0.041	0.002PheXaa: 0.002 ± 0.001
Gly	4.086GlyAla: 4.086 ± 0.062	0.929GlyCys: 0.929 ± 0.033	3.191GlyAsp: 3.191 ± 0.053	4.622GlyGlu: 4.622 ± 0.064	3.308GlyPhe: 3.308 ± 0.055	4.308GlyGly: 4.308 ± 0.079	1.077GlyHis: 1.077 ± 0.032	6.531GlyIle: 6.531 ± 0.082	5.457GlyLys: 5.457 ± 0.072	5.544GlyLeu: 5.544 ± 0.078	1.786GlyMet: 1.786 ± 0.039	3.26GlyAsn: 3.26 ± 0.058	1.23GlyPro: 1.23 ± 0.051	1.475GlyGln: 1.475 ± 0.033	2.376GlyArg: 2.376 ± 0.053	3.664GlySer: 3.664 ± 0.051	3.615GlyThr: 3.615 ± 0.057	4.761GlyVal: 4.761 ± 0.067	0.551GlyTrp: 0.551 ± 0.026	2.991GlyTyr: 2.991 ± 0.056	0.002GlyXaa: 0.002 ± 0.001
His	0.633HisAla: 0.633 ± 0.022	0.252HisCys: 0.252 ± 0.015	0.69HisAsp: 0.69 ± 0.023	1.036HisGlu: 1.036 ± 0.025	0.685HisPhe: 0.685 ± 0.023	1.132HisGly: 1.132 ± 0.033	0.361HisHis: 0.361 ± 0.021	1.415HisIle: 1.415 ± 0.032	1.205HisLys: 1.205 ± 0.032	1.262HisLeu: 1.262 ± 0.035	0.44HisMet: 0.44 ± 0.019	0.912HisAsn: 0.912 ± 0.028	0.601HisPro: 0.601 ± 0.021	0.322HisGln: 0.322 ± 0.015	0.665HisArg: 0.665 ± 0.021	0.94HisSer: 0.94 ± 0.028	0.691HisThr: 0.691 ± 0.022	0.817HisVal: 0.817 ± 0.028	0.131HisTrp: 0.131 ± 0.011	0.642HisTyr: 0.642 ± 0.021	0.002HisXaa: 0.002 ± 0.001
Ile	5.696IleAla: 5.696 ± 0.064	1.157IleCys: 1.157 ± 0.03	5.124IleAsp: 5.124 ± 0.059	7.04IleGlu: 7.04 ± 0.092	4.091IlePhe: 4.091 ± 0.064	5.994IleGly: 5.994 ± 0.074	1.316IleHis: 1.316 ± 0.031	8.68IleIle: 8.68 ± 0.105	8.105IleLys: 8.105 ± 0.093	8.679IleLeu: 8.679 ± 0.092	2.286IleMet: 2.286 ± 0.046	5.99IleAsn: 5.99 ± 0.066	3.141IlePro: 3.141 ± 0.052	2.232IleGln: 2.232 ± 0.043	3.032IleArg: 3.032 ± 0.048	6.747IleSer: 6.747 ± 0.081	5.064IleThr: 5.064 ± 0.07	5.867IleVal: 5.867 ± 0.072	0.545IleTrp: 0.545 ± 0.021	3.412IleTyr: 3.412 ± 0.054	0.001IleXaa: 0.001 ± 0.001
Lys	5.069LysAla: 5.069 ± 0.07	0.824LysCys: 0.824 ± 0.027	5.404LysAsp: 5.404 ± 0.061	8.872LysGlu: 8.872 ± 0.1	3.197LysPhe: 3.197 ± 0.059	5.282LysGly: 5.282 ± 0.073	1.193LysHis: 1.193 ± 0.029	7.16LysIle: 7.16 ± 0.09	7.198LysLys: 7.198 ± 0.093	7.488LysLeu: 7.488 ± 0.088	2.18LysMet: 2.18 ± 0.038	5.685LysAsn: 5.685 ± 0.066	2.201LysPro: 2.201 ± 0.042	2.21LysGln: 2.21 ± 0.041	3.029LysArg: 3.029 ± 0.047	5.041LysSer: 5.041 ± 0.067	4.08LysThr: 4.08 ± 0.06	6.369LysVal: 6.369 ± 0.077	0.601LysTrp: 0.601 ± 0.022	3.898LysTyr: 3.898 ± 0.059	0.003LysXaa: 0.003 ± 0.002
Leu	5.191LeuAla: 5.191 ± 0.074	1.195LeuCys: 1.195 ± 0.035	4.76LeuAsp: 4.76 ± 0.065	6.701LeuGlu: 6.701 ± 0.087	3.864LeuPhe: 3.864 ± 0.067	6.373LeuGly: 6.373 ± 0.082	1.297LeuHis: 1.297 ± 0.031	8.154LeuIle: 8.154 ± 0.101	8.505LeuLys: 8.505 ± 0.08	8.258LeuLeu: 8.258 ± 0.09	2.379LeuMet: 2.379 ± 0.04	5.75LeuAsn: 5.75 ± 0.072	2.842LeuPro: 2.842 ± 0.052	2.565LeuGln: 2.565 ± 0.043	3.384LeuArg: 3.384 ± 0.051	6.845LeuSer: 6.845 ± 0.085	4.689LeuThr: 4.689 ± 0.054	5.661LeuVal: 5.661 ± 0.073	0.671LeuTrp: 0.671 ± 0.028	3.427LeuTyr: 3.427 ± 0.056	0.002LeuXaa: 0.002 ± 0.001
Met	1.756MetAla: 1.756 ± 0.035	0.274MetCys: 0.274 ± 0.015	1.686MetAsp: 1.686 ± 0.036	2.163MetGlu: 2.163 ± 0.043	1.058MetPhe: 1.058 ± 0.032	1.795MetGly: 1.795 ± 0.041	0.345MetHis: 0.345 ± 0.017	2.134MetIle: 2.134 ± 0.053	2.588MetLys: 2.588 ± 0.043	2.295MetLeu: 2.295 ± 0.051	0.669MetMet: 0.669 ± 0.025	1.619MetAsn: 1.619 ± 0.035	0.927MetPro: 0.927 ± 0.03	0.619MetGln: 0.619 ± 0.025	0.98MetArg: 0.98 ± 0.025	1.645MetSer: 1.645 ± 0.034	1.142MetThr: 1.142 ± 0.028	1.835MetVal: 1.835 ± 0.037	0.183MetTrp: 0.183 ± 0.013	0.868MetTyr: 0.868 ± 0.028	0.001MetXaa: 0.001 ± 0.001
Asn	2.688AsnAla: 2.688 ± 0.048	0.763AsnCys: 0.763 ± 0.026	2.367AsnAsp: 2.367 ± 0.045	4.2AsnGlu: 4.2 ± 0.066	2.717AsnPhe: 2.717 ± 0.046	3.577AsnGly: 3.577 ± 0.062	0.945AsnHis: 0.945 ± 0.029	6.274AsnIle: 6.274 ± 0.074	5.649AsnLys: 5.649 ± 0.086	6.001AsnLeu: 6.001 ± 0.068	1.676AsnMet: 1.676 ± 0.044	4.173AsnAsn: 4.173 ± 0.072	2.148AsnPro: 2.148 ± 0.046	1.467AsnGln: 1.467 ± 0.033	2.027AsnArg: 2.027 ± 0.041	4.108AsnSer: 4.108 ± 0.068	3.012AsnThr: 3.012 ± 0.053	3.212AsnVal: 3.212 ± 0.047	0.448AsnTrp: 0.448 ± 0.019	2.776AsnTyr: 2.776 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	1.439ProAla: 1.439 ± 0.038	0.341ProCys: 0.341 ± 0.019	1.297ProAsp: 1.297 ± 0.036	2.286ProGlu: 2.286 ± 0.04	1.363ProPhe: 1.363 ± 0.033	1.661ProGly: 1.661 ± 0.043	0.468ProHis: 0.468 ± 0.018	2.6ProIle: 2.6 ± 0.052	2.252ProLys: 2.252 ± 0.042	2.528ProLeu: 2.528 ± 0.044	0.805ProMet: 0.805 ± 0.022	1.598ProAsn: 1.598 ± 0.037	0.673ProPro: 0.673 ± 0.029	0.936ProGln: 0.936 ± 0.03	0.916ProArg: 0.916 ± 0.028	1.843ProSer: 1.843 ± 0.039	1.655ProThr: 1.655 ± 0.037	2.157ProVal: 2.157 ± 0.048	0.261ProTrp: 0.261 ± 0.014	1.313ProTyr: 1.313 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	1.39GlnAla: 1.39 ± 0.039	0.372GlnCys: 0.372 ± 0.019	1.215GlnAsp: 1.215 ± 0.034	1.835GlnGlu: 1.835 ± 0.046	0.949GlnPhe: 0.949 ± 0.029	1.979GlnGly: 1.979 ± 0.051	0.389GlnHis: 0.389 ± 0.018	2.004GlnIle: 2.004 ± 0.043	1.896GlnLys: 1.896 ± 0.04	2.251GlnLeu: 2.251 ± 0.04	0.656GlnMet: 0.656 ± 0.024	1.346GlnAsn: 1.346 ± 0.034	0.618GlnPro: 0.618 ± 0.024	0.842GlnGln: 0.842 ± 0.03	1.092GlnArg: 1.092 ± 0.031	1.451GlnSer: 1.451 ± 0.036	1.06GlnThr: 1.06 ± 0.029	1.784GlnVal: 1.784 ± 0.037	0.373GlnTrp: 0.373 ± 0.018	1.109GlnTyr: 1.109 ± 0.03	0.002GlnXaa: 0.002 ± 0.001
Arg	1.983ArgAla: 1.983 ± 0.045	0.373ArgCys: 0.373 ± 0.018	1.846ArgAsp: 1.846 ± 0.04	3.24ArgGlu: 3.24 ± 0.056	1.607ArgPhe: 1.607 ± 0.04	2.196ArgGly: 2.196 ± 0.045	0.505ArgHis: 0.505 ± 0.021	3.147ArgIle: 3.147 ± 0.054	3.026ArgLys: 3.026 ± 0.051	3.033ArgLeu: 3.033 ± 0.05	0.914ArgMet: 0.914 ± 0.029	2.113ArgAsn: 2.113 ± 0.04	0.835ArgPro: 0.835 ± 0.028	0.925ArgGln: 0.925 ± 0.024	1.576ArgArg: 1.576 ± 0.04	1.62ArgSer: 1.62 ± 0.036	1.584ArgThr: 1.584 ± 0.036	2.441ArgVal: 2.441 ± 0.051	0.253ArgTrp: 0.253 ± 0.015	1.547ArgTyr: 1.547 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	3.189SerAla: 3.189 ± 0.054	0.654SerCys: 0.654 ± 0.024	2.813SerAsp: 2.813 ± 0.051	4.263SerGlu: 4.263 ± 0.057	3.203SerPhe: 3.203 ± 0.056	4.214SerGly: 4.214 ± 0.063	1.002SerHis: 1.002 ± 0.024	6.436SerIle: 6.436 ± 0.091	5.795SerLys: 5.795 ± 0.073	6.148SerLeu: 6.148 ± 0.075	1.829SerMet: 1.829 ± 0.038	3.707SerAsn: 3.707 ± 0.062	1.766SerPro: 1.766 ± 0.035	1.654SerGln: 1.654 ± 0.035	2.1SerArg: 2.1 ± 0.043	4.465SerSer: 4.465 ± 0.077	3.333SerThr: 3.333 ± 0.054	3.806SerVal: 3.806 ± 0.055	0.448SerTrp: 0.448 ± 0.023	2.691SerTyr: 2.691 ± 0.051	0.001SerXaa: 0.001 ± 0.001
Thr	2.997ThrAla: 2.997 ± 0.061	0.506ThrCys: 0.506 ± 0.023	2.147ThrAsp: 2.147 ± 0.047	3.274ThrGlu: 3.274 ± 0.052	2.38ThrPhe: 2.38 ± 0.044	3.557ThrGly: 3.557 ± 0.065	0.813ThrHis: 0.813 ± 0.025	4.889ThrIle: 4.889 ± 0.059	3.74ThrLys: 3.74 ± 0.059	5.344ThrLeu: 5.344 ± 0.083	1.305ThrMet: 1.305 ± 0.033	2.643ThrAsn: 2.643 ± 0.054	1.898ThrPro: 1.898 ± 0.039	1.156ThrGln: 1.156 ± 0.03	1.584ThrArg: 1.584 ± 0.035	3.376ThrSer: 3.376 ± 0.055	2.867ThrThr: 2.867 ± 0.055	3.612ThrVal: 3.612 ± 0.06	0.421ThrTrp: 0.421 ± 0.017	2.01ThrTyr: 2.01 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	4.153ValAla: 4.153 ± 0.066	0.822ValCys: 0.822 ± 0.027	3.652ValAsp: 3.652 ± 0.05	5.011ValGlu: 5.011 ± 0.067	3.04ValPhe: 3.04 ± 0.054	4.327ValGly: 4.327 ± 0.056	0.948ValHis: 0.948 ± 0.029	6.258ValIle: 6.258 ± 0.082	5.378ValLys: 5.378 ± 0.07	6.191ValLeu: 6.191 ± 0.078	1.713ValMet: 1.713 ± 0.039	3.68ValAsn: 3.68 ± 0.053	2.027ValPro: 2.027 ± 0.046	1.582ValGln: 1.582 ± 0.035	2.067ValArg: 2.067 ± 0.045	4.252ValSer: 4.252 ± 0.064	3.5ValThr: 3.5 ± 0.054	4.929ValVal: 4.929 ± 0.065	0.409ValTrp: 0.409 ± 0.016	2.524ValTyr: 2.524 ± 0.045	0.002ValXaa: 0.002 ± 0.001
Trp	0.36TrpAla: 0.36 ± 0.019	0.112TrpCys: 0.112 ± 0.009	0.417TrpAsp: 0.417 ± 0.016	0.505TrpGlu: 0.505 ± 0.019	0.334TrpPhe: 0.334 ± 0.018	0.557TrpGly: 0.557 ± 0.021	0.129TrpHis: 0.129 ± 0.01	0.646TrpIle: 0.646 ± 0.023	0.536TrpLys: 0.536 ± 0.022	0.674TrpLeu: 0.674 ± 0.026	0.207TrpMet: 0.207 ± 0.013	0.427TrpAsn: 0.427 ± 0.02	0.151TrpPro: 0.151 ± 0.01	0.24TrpGln: 0.24 ± 0.014	0.3TrpArg: 0.3 ± 0.016	0.426TrpSer: 0.426 ± 0.021	0.326TrpThr: 0.326 ± 0.017	0.484TrpVal: 0.484 ± 0.019	0.092TrpTrp: 0.092 ± 0.009	0.359TrpTyr: 0.359 ± 0.023	0.001TrpXaa: 0.001 ± 0.001
Tyr	1.876TyrAla: 1.876 ± 0.04	0.571TyrCys: 0.571 ± 0.025	2.399TyrAsp: 2.399 ± 0.049	3.106TyrGlu: 3.106 ± 0.051	2.22TyrPhe: 2.22 ± 0.041	2.745TyrGly: 2.745 ± 0.047	0.638TyrHis: 0.638 ± 0.023	3.875TyrIle: 3.875 ± 0.067	3.606TyrLys: 3.606 ± 0.06	3.868TyrLeu: 3.868 ± 0.055	1.013TyrMet: 1.013 ± 0.026	2.762TyrAsn: 2.762 ± 0.05	1.259TyrPro: 1.259 ± 0.032	0.795TyrGln: 0.795 ± 0.029	1.478TyrArg: 1.478 ± 0.03	2.857TyrSer: 2.857 ± 0.045	2.026TyrThr: 2.026 ± 0.042	2.494TyrVal: 2.494 ± 0.047	0.339TyrTrp: 0.339 ± 0.016	1.985TyrTyr: 1.985 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.003XaaGly: 0.003 ± 0.002	0.001XaaHis: 0.001 ± 0.001	0.002XaaIle: 0.002 ± 0.001	0.002XaaLys: 0.002 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.001

Statistics based on 4752 proteins (1254797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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