Acetobacterium bakii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Acetobacterium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3495 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6U3A1|A0A0L6U3A1_9FIRM Uroporphyrinogen decarboxylase OS=Acetobacterium bakii OX=52689 GN=AKG39_07750 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.44KK3 pKa = 9.3MSKK6 pKa = 10.14FMSLLMVLIMLSMLVPVSALAADD29 pKa = 3.95NEE31 pKa = 4.33EE32 pKa = 4.33FAATEE37 pKa = 4.01DD38 pKa = 3.64NAIKK42 pKa = 10.28IVLVTKK48 pKa = 10.15EE49 pKa = 3.88AAAEE53 pKa = 3.87DD54 pKa = 4.95AIVTDD59 pKa = 4.16DD60 pKa = 3.82PVIVEE65 pKa = 4.47EE66 pKa = 4.55PLVTDD71 pKa = 4.78EE72 pKa = 4.44IVVPAEE78 pKa = 4.21SSSDD82 pKa = 3.34TAVVPEE88 pKa = 4.34QPATTEE94 pKa = 3.97INTAAVPDD102 pKa = 4.14EE103 pKa = 4.81SIVTQMASYY112 pKa = 10.28LYY114 pKa = 10.99LMQNDD119 pKa = 3.36VMIDD123 pKa = 3.44VIAANTGADD132 pKa = 3.63YY133 pKa = 10.99SYY135 pKa = 11.49DD136 pKa = 3.35AAANQLTLNNFVGEE150 pKa = 4.17NMFVLSPADD159 pKa = 3.64TFKK162 pKa = 11.5LLLLGTNILRR172 pKa = 11.84TDD174 pKa = 2.74SGFAVNVYY182 pKa = 11.18GNMNSSGTGTLIAEE196 pKa = 4.73GLATEE201 pKa = 4.21NLMGGGINVIGNLVIDD217 pKa = 3.89SGSYY221 pKa = 10.63GIVAAGFEE229 pKa = 4.39NSSSAVGILTGYY241 pKa = 10.14NDD243 pKa = 6.12DD244 pKa = 5.13IITPGNLTINGGTIDD259 pKa = 3.27IAAYY263 pKa = 10.45NSDD266 pKa = 2.91IGGAFGLFAFGDD278 pKa = 4.27LIVNGGNIDD287 pKa = 3.36IFTNSPTSYY296 pKa = 10.64SRR298 pKa = 11.84GIGAYY303 pKa = 9.84DD304 pKa = 3.09KK305 pKa = 11.41LVFNGGNTTVDD316 pKa = 3.98SITDD320 pKa = 3.35TGFAEE325 pKa = 4.8ALYY328 pKa = 10.2SYY330 pKa = 9.85NTITINNGILDD341 pKa = 5.26LYY343 pKa = 10.68TSGASANALVAEE355 pKa = 4.49GGIYY359 pKa = 10.15INPSYY364 pKa = 11.56GDD366 pKa = 3.15VDD368 pKa = 4.98LNASSLYY375 pKa = 10.68LEE377 pKa = 4.52PLVTKK382 pKa = 10.19DD383 pKa = 3.7FKK385 pKa = 10.96QPHH388 pKa = 5.87YY389 pKa = 10.79SSSNPKK395 pKa = 9.47TGVDD399 pKa = 3.7TTAMDD404 pKa = 4.17ALVAGIMIFTLIGFSFAASKK424 pKa = 10.62RR425 pKa = 11.84GAKK428 pKa = 9.48AA429 pKa = 2.83

Molecular weight: 44.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6TXN6|A0A0L6TXN6_9FIRM Dihydrolipoyl dehydrogenase OS=Acetobacterium bakii OX=52689 GN=AKG39_14345 PE=3 SV=1
MM1 pKa = 7.87PKK3 pKa = 9.67MKK5 pKa = 8.76THH7 pKa = 6.92RR8 pKa = 11.84GAAKK12 pKa = 9.8RR13 pKa = 11.84FKK15 pKa = 9.92IKK17 pKa = 10.45KK18 pKa = 8.69SGAIKK23 pKa = 10.36RR24 pKa = 11.84FKK26 pKa = 10.49AGKK29 pKa = 8.89SHH31 pKa = 7.05ILNKK35 pKa = 9.96KK36 pKa = 6.79SRR38 pKa = 11.84KK39 pKa = 8.82RR40 pKa = 11.84KK41 pKa = 8.8RR42 pKa = 11.84NLRR45 pKa = 11.84KK46 pKa = 8.94ATGLAKK52 pKa = 10.46GDD54 pKa = 3.57AKK56 pKa = 10.48VVRR59 pKa = 11.84KK60 pKa = 8.96MISRR64 pKa = 4.2

Molecular weight: 7.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3495

0

3495

1076816

29

3271

308.1

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.2 ± 0.043

1.286 ± 0.018

5.54 ± 0.034

6.968 ± 0.048

4.451 ± 0.037

7.137 ± 0.042

1.688 ± 0.019

8.726 ± 0.041

7.007 ± 0.048

9.467 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.035 ± 0.02

4.862 ± 0.032

3.439 ± 0.025

3.099 ± 0.025

3.717 ± 0.034

5.789 ± 0.032

5.516 ± 0.042

6.691 ± 0.039

0.783 ± 0.013

3.599 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski