Maracuja mosaic virus
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0SEL4|A0SEL4_9VIRU Capsid protein OS=Maracuja mosaic virus OX=368736 GN=CP PE=3 SV=1
MM1 pKa = 8.18 PYY3 pKa = 10.35 QPITIKK9 pKa = 10.39 QLPWVSANWADD20 pKa = 3.71 YY21 pKa = 8.03 QTLVNVLRR29 pKa = 11.84 NTSATSFQTQAGRR42 pKa = 11.84 DD43 pKa = 3.85 SIRR46 pKa = 11.84 SQLTGCVDD54 pKa = 3.25 SAVQVNVRR62 pKa = 11.84 FPEE65 pKa = 4.12 RR66 pKa = 11.84 FLVFINDD73 pKa = 3.72 PAIADD78 pKa = 3.36 VWFALLKK85 pKa = 10.37 ATDD88 pKa = 3.56 TKK90 pKa = 11.41 NRR92 pKa = 11.84 IIEE95 pKa = 4.11 VDD97 pKa = 3.66 TEE99 pKa = 4.18 RR100 pKa = 11.84 SPSNSEE106 pKa = 3.59 IEE108 pKa = 4.49 SVTRR112 pKa = 11.84 RR113 pKa = 11.84 VDD115 pKa = 3.43 DD116 pKa = 4.03 ASVAIRR122 pKa = 11.84 INVEE126 pKa = 3.87 RR127 pKa = 11.84 LLKK130 pKa = 10.61 ALNDD134 pKa = 3.55 VQGVYY139 pKa = 10.86 DD140 pKa = 3.57 RR141 pKa = 11.84 TLFEE145 pKa = 4.28 QVSGLTWVEE154 pKa = 3.73 AGQVASASKK163 pKa = 10.73
Molecular weight: 18.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.183
IPC2_protein 5.296
IPC_protein 5.092
Toseland 4.927
ProMoST 5.194
Dawson 5.041
Bjellqvist 5.181
Wikipedia 4.914
Rodwell 4.927
Grimsley 4.838
Solomon 5.029
Lehninger 4.991
Nozaki 5.143
DTASelect 5.334
Thurlkill 4.927
EMBOSS 4.94
Sillero 5.194
Patrickios 4.571
IPC_peptide 5.041
IPC2_peptide 5.181
IPC2.peptide.svr19 5.34
Protein with the highest isoelectric point:
>tr|A0SEL4|A0SEL4_9VIRU Capsid protein OS=Maracuja mosaic virus OX=368736 GN=CP PE=3 SV=1
MM1 pKa = 7.74 SLRR4 pKa = 11.84 AQNAQDD10 pKa = 3.65 VVPPLDD16 pKa = 3.58 YY17 pKa = 11.12 LNPSNFVKK25 pKa = 10.53 PKK27 pKa = 10.45 LVGLEE32 pKa = 3.7 RR33 pKa = 11.84 LGLVKK38 pKa = 9.91 TPKK41 pKa = 10.31 KK42 pKa = 9.55 MCALMEE48 pKa = 4.21 TDD50 pKa = 3.77 VVKK53 pKa = 10.76 ISPKK57 pKa = 9.7 EE58 pKa = 3.71 RR59 pKa = 11.84 TVFPLNLVEE68 pKa = 4.92 KK69 pKa = 10.29 IKK71 pKa = 10.94 DD72 pKa = 3.26 IANYY76 pKa = 10.13 KK77 pKa = 10.73 YY78 pKa = 10.5 MMLLACVISGRR89 pKa = 11.84 WHH91 pKa = 5.84 VPNTVKK97 pKa = 11.14 GEE99 pKa = 4.18 VFLSLMDD106 pKa = 4.22 KK107 pKa = 10.69 RR108 pKa = 11.84 LTDD111 pKa = 3.46 EE112 pKa = 4.55 RR113 pKa = 11.84 EE114 pKa = 4.17 ATILTANANPSVSEE128 pKa = 3.81 FQIRR132 pKa = 11.84 IHH134 pKa = 6.71 PNYY137 pKa = 11.1 SMVASDD143 pKa = 5.78 AIQEE147 pKa = 4.06 PLEE150 pKa = 4.26 LFVHH154 pKa = 5.81 VKK156 pKa = 8.93 GLRR159 pKa = 11.84 MAQGFSPLSLEE170 pKa = 3.7 IAFCVVCCDD179 pKa = 3.62 VVIVKK184 pKa = 9.47 SLKK187 pKa = 9.94 MKK189 pKa = 10.37 ILQNQDD195 pKa = 2.45 KK196 pKa = 10.11 FGNAEE201 pKa = 4.04 GEE203 pKa = 4.43 VGSEE207 pKa = 4.21 GLDD210 pKa = 3.39 DD211 pKa = 5.18 LLRR214 pKa = 11.84 GSNMPSIRR222 pKa = 11.84 ASAVGSRR229 pKa = 11.84 KK230 pKa = 9.64 RR231 pKa = 11.84 LNANPKK237 pKa = 9.92 RR238 pKa = 11.84 VFSGSKK244 pKa = 9.32 GNSWSEE250 pKa = 3.56 KK251 pKa = 8.36 GNYY254 pKa = 9.0 VGVRR258 pKa = 11.84 KK259 pKa = 10.2 KK260 pKa = 10.76 KK261 pKa = 10.52 GVVEE265 pKa = 4.26 SDD267 pKa = 3.2 MLTSDD272 pKa = 4.58 AEE274 pKa = 4.63 STVSNYY280 pKa = 9.18 STDD283 pKa = 3.05 VSNALPTNNDD293 pKa = 2.83 QAVTMGVRR301 pKa = 11.84 QLGGLPNTGG310 pKa = 3.05
Molecular weight: 33.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.408
IPC2_protein 8.273
IPC_protein 8.185
Toseland 8.843
ProMoST 8.741
Dawson 9.165
Bjellqvist 9.077
Wikipedia 9.238
Rodwell 9.414
Grimsley 9.019
Solomon 9.282
Lehninger 9.268
Nozaki 9.326
DTASelect 8.946
Thurlkill 9.121
EMBOSS 9.355
Sillero 9.297
Patrickios 5.054
IPC_peptide 9.282
IPC2_peptide 7.732
IPC2.peptide.svr19 7.696
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
2056
163
1103
514.0
58.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.815 ± 1.064
1.946 ± 0.359
6.08 ± 0.387
5.837 ± 0.447
5.156 ± 0.725
5.204 ± 0.578
1.556 ± 0.367
5.253 ± 0.6
6.274 ± 0.759
8.998 ± 0.563
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.189 ± 0.542
5.107 ± 0.598
4.377 ± 0.247
3.745 ± 0.518
5.691 ± 0.514
7.539 ± 0.409
6.518 ± 0.99
9.29 ± 1.001
1.167 ± 0.279
3.259 ± 0.517
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here