Monilinia fructicola (Brown rot fungus) (Ciboria fructicola)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Monilinia

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13749 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M9JKD2|A0A5M9JKD2_MONFR Uncharacterized protein OS=Monilinia fructicola OX=38448 GN=EYC84_000790 PE=4 SV=1
MM1 pKa = 7.53SPNNPPSASSLQQEE15 pKa = 4.68TNQDD19 pKa = 3.5FPVLHH24 pKa = 5.65NRR26 pKa = 11.84KK27 pKa = 8.35MKK29 pKa = 10.05EE30 pKa = 3.57LEE32 pKa = 3.71RR33 pKa = 11.84EE34 pKa = 3.99YY35 pKa = 10.9RR36 pKa = 11.84STNRR40 pKa = 11.84TDD42 pKa = 3.47DD43 pKa = 3.46TQTNSRR49 pKa = 11.84GHH51 pKa = 5.93SAGAMAILQSFNGMKK66 pKa = 10.62GFMEE70 pKa = 4.62SYY72 pKa = 10.51GLKK75 pKa = 9.49IWLDD79 pKa = 3.42EE80 pKa = 4.3DD81 pKa = 3.79VQTARR86 pKa = 11.84QIINKK91 pKa = 7.72MLEE94 pKa = 3.67YY95 pKa = 10.62DD96 pKa = 3.81EE97 pKa = 4.35QHH99 pKa = 6.72GDD101 pKa = 3.39FGLLDD106 pKa = 3.93EE107 pKa = 5.18EE108 pKa = 4.84SADD111 pKa = 3.66GVISGRR117 pKa = 11.84EE118 pKa = 3.96FEE120 pKa = 6.02DD121 pKa = 3.46EE122 pKa = 4.14MEE124 pKa = 4.31DD125 pKa = 3.22TSRR128 pKa = 11.84IEE130 pKa = 3.84NTYY133 pKa = 11.16QNADD137 pKa = 3.66EE138 pKa = 4.45NNEE141 pKa = 3.83GSGMGNLDD149 pKa = 3.67LDD151 pKa = 3.82TRR153 pKa = 11.84QWEE156 pKa = 4.44VSHH159 pKa = 6.4GEE161 pKa = 4.27RR162 pKa = 11.84YY163 pKa = 9.49CVPDD167 pKa = 3.82EE168 pKa = 5.41LEE170 pKa = 4.67GDD172 pKa = 4.06DD173 pKa = 5.22VGGDD177 pKa = 3.99CEE179 pKa = 4.76GSCNRR184 pKa = 11.84EE185 pKa = 3.65EE186 pKa = 4.35NFDD189 pKa = 3.49VSIVDD194 pKa = 4.01RR195 pKa = 11.84EE196 pKa = 4.27EE197 pKa = 4.88GCDD200 pKa = 3.41DD201 pKa = 3.92YY202 pKa = 12.03GFGGCEE208 pKa = 4.63DD209 pKa = 5.09DD210 pKa = 5.0GQGCYY215 pKa = 10.65GDD217 pKa = 5.77DD218 pKa = 3.76YY219 pKa = 11.64DD220 pKa = 6.3GNDD223 pKa = 3.93CEE225 pKa = 5.34GYY227 pKa = 10.71DD228 pKa = 3.72YY229 pKa = 11.6GRR231 pKa = 11.84DD232 pKa = 4.24DD233 pKa = 5.55DD234 pKa = 4.17EE235 pKa = 6.98CDD237 pKa = 4.01YY238 pKa = 11.99GDD240 pKa = 5.25DD241 pKa = 3.67NCDD244 pKa = 3.3YY245 pKa = 11.36DD246 pKa = 5.15SYY248 pKa = 12.13

Molecular weight: 28.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M9K7V6|A0A5M9K7V6_MONFR Long-chain-alcohol oxidase OS=Monilinia fructicola OX=38448 GN=EYC84_006993 PE=3 SV=1
MM1 pKa = 7.56TISPSQAPTKK11 pKa = 10.15IDD13 pKa = 3.81TIPISPYY20 pKa = 9.82HH21 pKa = 6.51ALDD24 pKa = 4.22LSFLCASPSPGNSPSHH40 pKa = 6.47AAQIGKK46 pKa = 8.74PRR48 pKa = 11.84ASHH51 pKa = 6.76LSVPPPRR58 pKa = 11.84MDD60 pKa = 5.08LKK62 pKa = 9.95PTPHH66 pKa = 7.14CCTAPYY72 pKa = 10.08HH73 pKa = 6.46IFPTMRR79 pKa = 11.84LYY81 pKa = 9.54RR82 pKa = 11.84THH84 pKa = 6.64HH85 pKa = 6.49HH86 pKa = 6.97PPDD89 pKa = 3.6THH91 pKa = 6.73HH92 pKa = 7.13KK93 pKa = 10.04LHH95 pKa = 6.55TALASPTQNPNPPHH109 pKa = 7.56RR110 pKa = 11.84IRR112 pKa = 11.84IPIPIPKK119 pKa = 8.98PSHH122 pKa = 5.21TRR124 pKa = 11.84SPAKK128 pKa = 10.37KK129 pKa = 9.15EE130 pKa = 3.91AVQALPPPPSRR141 pKa = 11.84RR142 pKa = 11.84NNTNPRR148 pKa = 11.84HH149 pKa = 6.65RR150 pKa = 11.84IAQTPKK156 pKa = 10.27DD157 pKa = 3.74AKK159 pKa = 9.43KK160 pKa = 10.37CPSIIPAMRR169 pKa = 11.84RR170 pKa = 11.84HH171 pKa = 6.45PNPDD175 pKa = 2.92SCFMARR181 pKa = 11.84HH182 pKa = 5.8LPFLRR187 pKa = 11.84CMFHH191 pKa = 5.95GQRR194 pKa = 11.84GSICTSIPTLYY205 pKa = 9.54TLHH208 pKa = 6.0ITHH211 pKa = 6.56YY212 pKa = 9.4PFQPPMQHH220 pKa = 4.51RR221 pKa = 11.84HH222 pKa = 3.64MAIFRR227 pKa = 11.84VNHH230 pKa = 5.49ARR232 pKa = 11.84VNVTLPSFPPPSFPPPFLPRR252 pKa = 11.84HH253 pKa = 6.19PPPQQDD259 pKa = 2.94IALCAASPEE268 pKa = 4.23PKK270 pKa = 9.57HH271 pKa = 6.34PKK273 pKa = 9.54IIHH276 pKa = 6.28PCNDD280 pKa = 3.1TRR282 pKa = 11.84CDD284 pKa = 3.69AMGSDD289 pKa = 3.44ATRR292 pKa = 11.84RR293 pKa = 11.84DD294 pKa = 3.68TRR296 pKa = 11.84AHH298 pKa = 5.55PQKK301 pKa = 10.69SEE303 pKa = 3.78PRR305 pKa = 11.84TNSCPSVPLSPLPFLHH321 pKa = 7.16ASCFTCTSPLRR332 pKa = 11.84QPSPPRR338 pKa = 11.84RR339 pKa = 11.84TCKK342 pKa = 9.96PQAKK346 pKa = 10.01ASTIPLRR353 pKa = 11.84FHH355 pKa = 6.72RR356 pKa = 11.84VSRR359 pKa = 11.84DD360 pKa = 3.48PSVHH364 pKa = 6.18LANLTGTTRR373 pKa = 11.84PTSSNGSRR381 pKa = 11.84APDD384 pKa = 3.04RR385 pKa = 11.84MHH387 pKa = 7.1ALFSFIYY394 pKa = 9.3EE395 pKa = 4.18FYY397 pKa = 10.62ISIHH401 pKa = 6.25ASSAVGAIVQLLGCLNPNPRR421 pKa = 11.84PRR423 pKa = 11.84LNSNHH428 pKa = 6.01

Molecular weight: 47.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12118

1631

13749

5524529

66

6810

401.8

44.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.694 ± 0.019

1.201 ± 0.008

5.446 ± 0.016

6.429 ± 0.029

3.708 ± 0.014

6.795 ± 0.025

2.414 ± 0.01

5.473 ± 0.017

5.387 ± 0.021

8.535 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.008

4.165 ± 0.013

5.978 ± 0.025

3.905 ± 0.018

5.85 ± 0.02

8.862 ± 0.031

6.127 ± 0.017

5.67 ± 0.015

1.36 ± 0.007

2.79 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski