Miscanthus streak virus (isolate 91) (MiSV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Miscanthus streak virus

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q67593|MP_MISV9 Putative movement protein OS=Miscanthus streak virus (isolate 91) OX=268776 GN=V2 PE=3 SV=1
MM1 pKa = 7.98DD2 pKa = 5.91PYY4 pKa = 11.13GSRR7 pKa = 11.84PSHH10 pKa = 6.92PDD12 pKa = 2.94DD13 pKa = 4.57GALHH17 pKa = 6.99GILVAFIAVLCLIGCLWAAYY37 pKa = 10.2RR38 pKa = 11.84LFLKK42 pKa = 10.51EE43 pKa = 4.61CLTDD47 pKa = 4.21CSQHH51 pKa = 5.35TSSGVVAGPRR61 pKa = 11.84PAATGPTAVVVHH73 pKa = 6.6NGAEE77 pKa = 4.24AQRR80 pKa = 11.84SSAFF84 pKa = 3.32

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q67593|MP_MISV9 Putative movement protein OS=Miscanthus streak virus (isolate 91) OX=268776 GN=V2 PE=3 SV=1
MM1 pKa = 7.33GCVFIMGRR9 pKa = 11.84RR10 pKa = 11.84GSEE13 pKa = 4.19TNNITAPPPWPISRR27 pKa = 11.84PLASVRR33 pKa = 11.84CAFGPRR39 pKa = 11.84SLPPLAEE46 pKa = 4.96DD47 pKa = 3.53RR48 pKa = 11.84TQAAVKK54 pKa = 7.66WHH56 pKa = 6.09RR57 pKa = 11.84HH58 pKa = 4.12LSVFALVPLVCGFKK72 pKa = 10.27KK73 pKa = 10.08RR74 pKa = 11.84RR75 pKa = 11.84RR76 pKa = 11.84LRR78 pKa = 11.84DD79 pKa = 3.21RR80 pKa = 11.84TKK82 pKa = 10.46TKK84 pKa = 9.09GTQVACLRR92 pKa = 11.84TWVIKK97 pKa = 10.49FCFVCKK103 pKa = 10.08FLWIPMVAVLAILMMVHH120 pKa = 6.13CMVYY124 pKa = 9.81LWHH127 pKa = 7.44LLRR130 pKa = 11.84CFASS134 pKa = 3.38

Molecular weight: 15.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1038

84

348

207.6

23.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.033 ± 1.048

2.216 ± 0.699

4.721 ± 0.529

4.239 ± 1.065

4.817 ± 0.451

5.491 ± 0.909

2.697 ± 0.438

5.106 ± 0.603

6.262 ± 0.742

6.744 ± 0.773

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.295

4.432 ± 0.719

8.189 ± 0.904

4.335 ± 0.648

6.262 ± 0.631

6.262 ± 0.427

6.166 ± 0.966

6.936 ± 1.165

2.119 ± 0.364

3.276 ± 0.615
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski