Massilia timonae CCUG 45783

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; Massilia timonae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5096 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9DBE1|K9DBE1_9BURK HTH lysR-type domain-containing protein OS=Massilia timonae CCUG 45783 OX=883126 GN=HMPREF9710_02687 PE=4 SV=1
MM1 pKa = 6.79TAVAEE6 pKa = 4.31VQDD9 pKa = 4.51FDD11 pKa = 4.94TIPVPINFTDD21 pKa = 3.53SAAQKK26 pKa = 9.24VAQLIEE32 pKa = 4.37EE33 pKa = 4.51EE34 pKa = 4.63GNPDD38 pKa = 2.85LKK40 pKa = 11.13LRR42 pKa = 11.84VFVQGGGCSGFQYY55 pKa = 10.94GFTFDD60 pKa = 4.69EE61 pKa = 4.93IVNEE65 pKa = 4.96DD66 pKa = 3.85DD67 pKa = 3.19TTMEE71 pKa = 4.19KK72 pKa = 10.82NGVQLLIDD80 pKa = 3.77SMSYY84 pKa = 10.23QYY86 pKa = 11.55LVGAEE91 pKa = 4.01IDD93 pKa = 3.89YY94 pKa = 11.28KK95 pKa = 11.39DD96 pKa = 4.47DD97 pKa = 4.08LEE99 pKa = 4.56GAQFVIKK106 pKa = 10.53NPNATSTCGCGSSFSAA122 pKa = 4.79

Molecular weight: 13.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9DBC5|K9DBC5_9BURK Protein-tyrosine-phosphatase OS=Massilia timonae CCUG 45783 OX=883126 GN=HMPREF9710_04115 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 10.37APQFEE7 pKa = 4.56KK8 pKa = 10.99LFAQLALLNQPQHH21 pKa = 6.04RR22 pKa = 11.84QVLVALHH29 pKa = 6.6PAAGLDD35 pKa = 3.27RR36 pKa = 11.84VVALIGEE43 pKa = 4.29IRR45 pKa = 11.84SKK47 pKa = 10.94GRR49 pKa = 11.84RR50 pKa = 11.84CPDD53 pKa = 3.54CACDD57 pKa = 3.02RR58 pKa = 11.84CHH60 pKa = 7.38RR61 pKa = 11.84HH62 pKa = 5.26GQANDD67 pKa = 3.32LQRR70 pKa = 11.84YY71 pKa = 6.7RR72 pKa = 11.84CCACGRR78 pKa = 11.84TFNDD82 pKa = 3.34LTGTPLARR90 pKa = 11.84LRR92 pKa = 11.84HH93 pKa = 5.26KK94 pKa = 10.61GKK96 pKa = 9.19WLEE99 pKa = 4.03YY100 pKa = 10.25LDD102 pKa = 4.23TVLDD106 pKa = 3.81SRR108 pKa = 11.84TVRR111 pKa = 11.84SAAKK115 pKa = 10.1RR116 pKa = 11.84IGVHH120 pKa = 6.59RR121 pKa = 11.84NTTFRR126 pKa = 11.84WRR128 pKa = 11.84HH129 pKa = 4.41RR130 pKa = 11.84VLDD133 pKa = 3.8RR134 pKa = 11.84VKK136 pKa = 10.87DD137 pKa = 3.78DD138 pKa = 4.53RR139 pKa = 11.84PDD141 pKa = 3.36HH142 pKa = 6.22LVGIVEE148 pKa = 4.21ADD150 pKa = 3.3EE151 pKa = 4.41MFLLEE156 pKa = 4.14SQKK159 pKa = 10.8GSRR162 pKa = 11.84KK163 pKa = 9.53LDD165 pKa = 3.32RR166 pKa = 11.84PPRR169 pKa = 11.84KK170 pKa = 9.42RR171 pKa = 11.84GGRR174 pKa = 11.84AALRR178 pKa = 11.84GISHH182 pKa = 7.15HH183 pKa = 6.89LDD185 pKa = 4.37CILVARR191 pKa = 11.84DD192 pKa = 3.6RR193 pKa = 11.84GGQTIDD199 pKa = 3.03AVTGRR204 pKa = 11.84GALKK208 pKa = 9.66VAQLVTHH215 pKa = 7.06LLPKK219 pKa = 10.5LHH221 pKa = 6.51AQALLVTDD229 pKa = 5.2ANTAYY234 pKa = 9.78PAFARR239 pKa = 11.84AHH241 pKa = 5.58GVAHH245 pKa = 5.58QAVNLSAGEE254 pKa = 4.04RR255 pKa = 11.84VRR257 pKa = 11.84KK258 pKa = 9.79GDD260 pKa = 3.11AGAIHH265 pKa = 5.77VQNVNAYY272 pKa = 9.0HH273 pKa = 6.79RR274 pKa = 11.84RR275 pKa = 11.84FKK277 pKa = 10.56EE278 pKa = 3.84WLAHH282 pKa = 4.55FHH284 pKa = 6.16GVASRR289 pKa = 11.84WLPNYY294 pKa = 10.14LGWHH298 pKa = 5.85WALDD302 pKa = 3.7GGRR305 pKa = 11.84VNSVEE310 pKa = 3.79QLLRR314 pKa = 11.84IAFGVINSS322 pKa = 3.73

Molecular weight: 36.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5096

0

5096

1765012

23

2700

346.4

37.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.736 ± 0.052

0.858 ± 0.011

5.575 ± 0.023

5.388 ± 0.029

3.516 ± 0.02

8.393 ± 0.028

2.238 ± 0.017

4.513 ± 0.026

3.305 ± 0.034

10.465 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.487 ± 0.015

2.894 ± 0.022

5.145 ± 0.023

3.75 ± 0.022

7.101 ± 0.035

5.325 ± 0.027

5.07 ± 0.028

7.418 ± 0.027

1.377 ± 0.013

2.444 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski