Acetivibrio ethanolgignens
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3375 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0V8QIE0|A0A0V8QIE0_9FIRM Chloride channel protein OS=Acetivibrio ethanolgignens OX=290052 GN=ASU35_16885 PE=4 SV=1
MM1 pKa = 7.32 NKK3 pKa = 10.07 AVIYY7 pKa = 10.52 ISGTMNEE14 pKa = 4.56 TVDD17 pKa = 3.52 NMVVRR22 pKa = 11.84 VIEE25 pKa = 4.58 RR26 pKa = 11.84 YY27 pKa = 9.22 CEE29 pKa = 3.63 KK30 pKa = 10.41 HH31 pKa = 6.07 GYY33 pKa = 9.25 EE34 pKa = 3.74 IAALLGEE41 pKa = 4.63 SSQSGMSMPMKK52 pKa = 10.46 YY53 pKa = 10.44 SFIGMAEE60 pKa = 3.94 VEE62 pKa = 4.88 DD63 pKa = 4.08 IDD65 pKa = 4.35 TVVTLSSAMVGTTDD79 pKa = 4.22 DD80 pKa = 4.89 EE81 pKa = 4.49 IMEE84 pKa = 4.81 TIDD87 pKa = 4.52 LLAHH91 pKa = 5.72 YY92 pKa = 9.19 GITVEE97 pKa = 4.2 TAKK100 pKa = 10.91 EE101 pKa = 3.91 DD102 pKa = 3.25 MSAYY106 pKa = 10.38 YY107 pKa = 10.17 DD108 pKa = 3.55 ALGSDD113 pKa = 5.44 GYY115 pKa = 10.76 CGCDD119 pKa = 3.73 DD120 pKa = 4.83 SSMDD124 pKa = 3.26 DD125 pKa = 3.38 TQIIKK130 pKa = 10.43 EE131 pKa = 4.06 LDD133 pKa = 3.05 KK134 pKa = 10.95 LIRR137 pKa = 11.84 MVSGRR142 pKa = 11.84 QQDD145 pKa = 3.07
Molecular weight: 16.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.811
IPC2_protein 4.037
IPC_protein 4.012
Toseland 3.808
ProMoST 4.101
Dawson 3.986
Bjellqvist 4.177
Wikipedia 3.897
Rodwell 3.834
Grimsley 3.719
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.304
Thurlkill 3.846
EMBOSS 3.91
Sillero 4.126
Patrickios 1.926
IPC_peptide 3.973
IPC2_peptide 4.101
IPC2.peptide.svr19 4.027
Protein with the highest isoelectric point:
>tr|A0A0V8QEF9|A0A0V8QEF9_9FIRM Adenine phosphoribosyltransferase OS=Acetivibrio ethanolgignens OX=290052 GN=apt PE=3 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 9.06 KK9 pKa = 8.03 RR10 pKa = 11.84 SRR12 pKa = 11.84 SKK14 pKa = 9.44 VHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.19 NGRR28 pKa = 11.84 KK29 pKa = 8.98 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.71 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 5.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3375
0
3375
1009164
37
2290
299.0
33.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.124 ± 0.047
1.449 ± 0.017
5.335 ± 0.037
8.265 ± 0.052
4.116 ± 0.033
6.814 ± 0.041
1.597 ± 0.017
7.547 ± 0.037
7.555 ± 0.035
9.232 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.911 ± 0.024
4.466 ± 0.033
3.075 ± 0.022
3.136 ± 0.023
4.457 ± 0.035
5.808 ± 0.039
5.338 ± 0.052
6.68 ± 0.036
0.887 ± 0.012
4.207 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here