Enterobacteria phage vB_EcoS_Rogue1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Rogunavirinae; Rogunavirus; Escherichia virus Rogue1

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7PK35|K7PK35_9CAUD Uncharacterized protein OS=Enterobacteria phage vB_EcoS_Rogue1 OX=1147155 GN=Rogue1_0039 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.38INLLCTNAEE11 pKa = 4.01VGVYY15 pKa = 9.96DD16 pKa = 4.35LNVGKK21 pKa = 9.01TYY23 pKa = 10.76VAYY26 pKa = 10.7FNDD29 pKa = 3.72DD30 pKa = 3.03YY31 pKa = 11.65GYY33 pKa = 11.41YY34 pKa = 9.8EE35 pKa = 4.81LSDD38 pKa = 3.29EE39 pKa = 5.07GFFLL43 pKa = 5.42

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7PL75|K7PL75_9CAUD Uncharacterized protein OS=Enterobacteria phage vB_EcoS_Rogue1 OX=1147155 GN=Rogue1_0053 PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 6.16SIDD5 pKa = 4.34KK6 pKa = 9.33RR7 pKa = 11.84TLNGNNGTIRR17 pKa = 11.84TEE19 pKa = 3.92DD20 pKa = 3.06KK21 pKa = 10.74KK22 pKa = 10.7PRR24 pKa = 11.84KK25 pKa = 9.27RR26 pKa = 11.84PSGYY30 pKa = 10.17YY31 pKa = 8.95VLKK34 pKa = 10.9DD35 pKa = 3.51EE36 pKa = 4.52VRR38 pKa = 11.84AGLRR42 pKa = 11.84ARR44 pKa = 11.84LEE46 pKa = 4.11IVLDD50 pKa = 4.33FYY52 pKa = 11.64GSKK55 pKa = 11.11ANLAKK60 pKa = 10.04QLKK63 pKa = 6.67VTRR66 pKa = 11.84QAVEE70 pKa = 4.15EE71 pKa = 4.13WCKK74 pKa = 10.82RR75 pKa = 11.84GMISARR81 pKa = 11.84GAQLAHH87 pKa = 5.93NRR89 pKa = 11.84YY90 pKa = 9.16KK91 pKa = 10.35RR92 pKa = 11.84TGEE95 pKa = 3.84GFRR98 pKa = 11.84ATFCRR103 pKa = 11.84PDD105 pKa = 3.38LQFDD109 pKa = 4.36GNGKK113 pKa = 8.92PLTLRR118 pKa = 11.84CKK120 pKa = 9.57KK121 pKa = 10.07RR122 pKa = 11.84QMLRR126 pKa = 11.84VVTEE130 pKa = 4.28AEE132 pKa = 4.41LATKK136 pKa = 8.4PQRR139 pKa = 11.84KK140 pKa = 7.72SWRR143 pKa = 11.84KK144 pKa = 7.89VKK146 pKa = 10.54KK147 pKa = 9.7EE148 pKa = 3.59RR149 pKa = 11.84EE150 pKa = 3.98EE151 pKa = 3.69ARR153 pKa = 11.84KK154 pKa = 10.06AKK156 pKa = 9.88EE157 pKa = 3.53

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13988

28

1128

189.0

20.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.364 ± 0.444

1.315 ± 0.166

6.277 ± 0.199

6.277 ± 0.387

3.889 ± 0.145

7.885 ± 0.478

1.43 ± 0.177

6.355 ± 0.181

6.22 ± 0.463

7.178 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.233

5.076 ± 0.282

3.181 ± 0.297

3.953 ± 0.282

4.804 ± 0.206

7.228 ± 0.429

5.719 ± 0.322

7.12 ± 0.284

1.208 ± 0.106

3.932 ± 0.258
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski