Amino acid dipepetide frequency for Enterobacteria phage vB_EcoS_Rogue1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.222AlaAla: 8.222 ± 0.991
0.5AlaCys: 0.5 ± 0.249
3.861AlaAsp: 3.861 ± 0.758
4.862AlaGlu: 4.862 ± 0.787
2.645AlaPhe: 2.645 ± 0.466
7.864AlaGly: 7.864 ± 1.191
1.144AlaHis: 1.144 ± 0.308
6.506AlaIle: 6.506 ± 0.761
6.363AlaLys: 6.363 ± 1.296
6.935AlaLeu: 6.935 ± 0.795
2.288AlaMet: 2.288 ± 0.428
3.646AlaAsn: 3.646 ± 0.544
1.573AlaPro: 1.573 ± 0.387
3.646AlaGln: 3.646 ± 0.526
4.504AlaArg: 4.504 ± 0.681
5.076AlaSer: 5.076 ± 0.662
4.79AlaThr: 4.79 ± 0.675
5.076AlaVal: 5.076 ± 0.617
1.358AlaTrp: 1.358 ± 0.36
2.788AlaTyr: 2.788 ± 0.425
0.0AlaXaa: 0.0 ± 0.0
Cys
0.643CysAla: 0.643 ± 0.25
0.143CysCys: 0.143 ± 0.114
0.786CysAsp: 0.786 ± 0.202
0.929CysGlu: 0.929 ± 0.25
0.572CysPhe: 0.572 ± 0.231
1.001CysGly: 1.001 ± 0.272
0.214CysHis: 0.214 ± 0.146
0.572CysIle: 0.572 ± 0.221
1.001CysLys: 1.001 ± 0.283
1.001CysLeu: 1.001 ± 0.276
0.429CysMet: 0.429 ± 0.181
0.643CysAsn: 0.643 ± 0.183
0.429CysPro: 0.429 ± 0.188
0.429CysGln: 0.429 ± 0.158
0.858CysArg: 0.858 ± 0.219
1.144CysSer: 1.144 ± 0.375
0.643CysThr: 0.643 ± 0.224
1.001CysVal: 1.001 ± 0.283
0.214CysTrp: 0.214 ± 0.146
0.5CysTyr: 0.5 ± 0.206
0.0CysXaa: 0.0 ± 0.0
Asp
4.79AspAla: 4.79 ± 0.786
0.5AspCys: 0.5 ± 0.244
4.29AspAsp: 4.29 ± 0.611
4.719AspGlu: 4.719 ± 0.624
2.431AspPhe: 2.431 ± 0.398
7.149AspGly: 7.149 ± 0.799
0.715AspHis: 0.715 ± 0.255
3.718AspIle: 3.718 ± 0.448
5.434AspLys: 5.434 ± 0.612
3.861AspLeu: 3.861 ± 0.548
1.287AspMet: 1.287 ± 0.278
2.431AspAsn: 2.431 ± 0.465
1.859AspPro: 1.859 ± 0.313
1.43AspGln: 1.43 ± 0.418
2.216AspArg: 2.216 ± 0.471
4.433AspSer: 4.433 ± 0.65
3.36AspThr: 3.36 ± 0.406
4.004AspVal: 4.004 ± 0.521
0.715AspTrp: 0.715 ± 0.264
3.432AspTyr: 3.432 ± 0.482
0.0AspXaa: 0.0 ± 0.0
Glu
5.362GluAla: 5.362 ± 0.862
0.929GluCys: 0.929 ± 0.29
3.217GluAsp: 3.217 ± 0.482
3.718GluGlu: 3.718 ± 0.62
3.575GluPhe: 3.575 ± 0.541
3.289GluGly: 3.289 ± 0.508
0.786GluHis: 0.786 ± 0.261
4.862GluIle: 4.862 ± 0.569
3.289GluLys: 3.289 ± 0.665
5.934GluLeu: 5.934 ± 0.593
2.288GluMet: 2.288 ± 0.491
2.86GluAsn: 2.86 ± 0.534
1.001GluPro: 1.001 ± 0.234
2.788GluGln: 2.788 ± 0.473
3.003GluArg: 3.003 ± 0.566
4.004GluSer: 4.004 ± 0.638
4.004GluThr: 4.004 ± 0.468
5.005GluVal: 5.005 ± 0.761
0.5GluTrp: 0.5 ± 0.205
2.359GluTyr: 2.359 ± 0.461
0.0GluXaa: 0.0 ± 0.0
Phe
2.931PheAla: 2.931 ± 0.523
0.715PheCys: 0.715 ± 0.227
3.646PheAsp: 3.646 ± 0.522
2.431PheGlu: 2.431 ± 0.489
0.929PhePhe: 0.929 ± 0.265
4.361PheGly: 4.361 ± 0.562
0.5PheHis: 0.5 ± 0.196
2.073PheIle: 2.073 ± 0.264
2.002PheLys: 2.002 ± 0.382
2.431PheLeu: 2.431 ± 0.425
1.001PheMet: 1.001 ± 0.256
2.502PheAsn: 2.502 ± 0.515
1.215PhePro: 1.215 ± 0.292
1.644PheGln: 1.644 ± 0.363
1.644PheArg: 1.644 ± 0.364
2.431PheSer: 2.431 ± 0.383
2.288PheThr: 2.288 ± 0.385
2.216PheVal: 2.216 ± 0.312
0.5PheTrp: 0.5 ± 0.18
1.43PheTyr: 1.43 ± 0.339
0.0PheXaa: 0.0 ± 0.0
Gly
4.647GlyAla: 4.647 ± 0.816
1.287GlyCys: 1.287 ± 0.315
4.647GlyAsp: 4.647 ± 0.677
5.791GlyGlu: 5.791 ± 0.635
3.289GlyPhe: 3.289 ± 0.807
6.22GlyGly: 6.22 ± 1.013
0.786GlyHis: 0.786 ± 0.315
5.219GlyIle: 5.219 ± 0.555
4.933GlyLys: 4.933 ± 0.739
6.649GlyLeu: 6.649 ± 0.705
2.145GlyMet: 2.145 ± 0.458
3.432GlyAsn: 3.432 ± 0.518
2.931GlyPro: 2.931 ± 2.328
1.43GlyGln: 1.43 ± 0.336
2.931GlyArg: 2.931 ± 0.432
6.149GlySer: 6.149 ± 0.688
4.647GlyThr: 4.647 ± 0.732
6.578GlyVal: 6.578 ± 0.65
1.358GlyTrp: 1.358 ± 0.377
3.789GlyTyr: 3.789 ± 0.506
0.0GlyXaa: 0.0 ± 0.0
His
0.858HisAla: 0.858 ± 0.265
0.214HisCys: 0.214 ± 0.107
1.001HisAsp: 1.001 ± 0.3
0.858HisGlu: 0.858 ± 0.287
0.643HisPhe: 0.643 ± 0.226
1.215HisGly: 1.215 ± 0.331
0.5HisHis: 0.5 ± 0.211
1.001HisIle: 1.001 ± 0.293
1.644HisLys: 1.644 ± 0.428
1.215HisLeu: 1.215 ± 0.326
0.214HisMet: 0.214 ± 0.114
0.643HisAsn: 0.643 ± 0.273
0.214HisPro: 0.214 ± 0.118
0.286HisGln: 0.286 ± 0.142
0.715HisArg: 0.715 ± 0.207
0.715HisSer: 0.715 ± 0.266
0.715HisThr: 0.715 ± 0.212
1.001HisVal: 1.001 ± 0.242
0.071HisTrp: 0.071 ± 0.079
0.572HisTyr: 0.572 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.362IleAla: 5.362 ± 0.695
1.072IleCys: 1.072 ± 0.265
5.505IleAsp: 5.505 ± 0.752
4.933IleGlu: 4.933 ± 0.719
1.93IlePhe: 1.93 ± 0.258
3.932IleGly: 3.932 ± 0.6
1.072IleHis: 1.072 ± 0.265
3.36IleIle: 3.36 ± 0.528
3.575IleLys: 3.575 ± 0.427
3.432IleLeu: 3.432 ± 0.593
1.215IleMet: 1.215 ± 0.341
3.932IleAsn: 3.932 ± 0.576
2.645IlePro: 2.645 ± 0.476
2.288IleGln: 2.288 ± 0.507
3.718IleArg: 3.718 ± 0.564
4.075IleSer: 4.075 ± 0.543
4.361IleThr: 4.361 ± 0.522
3.646IleVal: 3.646 ± 0.404
0.572IleTrp: 0.572 ± 0.19
2.931IleTyr: 2.931 ± 0.493
0.0IleXaa: 0.0 ± 0.0
Lys
6.149LysAla: 6.149 ± 1.123
0.572LysCys: 0.572 ± 0.318
3.789LysAsp: 3.789 ± 0.45
4.147LysGlu: 4.147 ± 0.862
2.502LysPhe: 2.502 ± 0.447
3.432LysGly: 3.432 ± 0.549
1.287LysHis: 1.287 ± 0.263
3.932LysIle: 3.932 ± 0.471
3.861LysLys: 3.861 ± 0.68
5.505LysLeu: 5.505 ± 0.873
2.574LysMet: 2.574 ± 0.544
2.002LysAsn: 2.002 ± 0.397
1.287LysPro: 1.287 ± 0.401
2.788LysGln: 2.788 ± 0.468
2.359LysArg: 2.359 ± 0.567
4.576LysSer: 4.576 ± 0.527
3.575LysThr: 3.575 ± 0.448
4.719LysVal: 4.719 ± 0.599
0.715LysTrp: 0.715 ± 0.243
2.359LysTyr: 2.359 ± 0.394
0.0LysXaa: 0.0 ± 0.0
Leu
6.435LeuAla: 6.435 ± 0.662
0.929LeuCys: 0.929 ± 0.28
4.862LeuAsp: 4.862 ± 0.586
3.932LeuGlu: 3.932 ± 0.563
2.216LeuPhe: 2.216 ± 0.339
4.719LeuGly: 4.719 ± 0.692
1.358LeuHis: 1.358 ± 0.356
3.718LeuIle: 3.718 ± 0.604
4.147LeuLys: 4.147 ± 0.501
4.576LeuLeu: 4.576 ± 0.594
2.002LeuMet: 2.002 ± 0.362
3.718LeuAsn: 3.718 ± 0.394
3.432LeuPro: 3.432 ± 0.49
2.86LeuGln: 2.86 ± 0.667
3.932LeuArg: 3.932 ± 0.644
6.649LeuSer: 6.649 ± 0.711
5.005LeuThr: 5.005 ± 0.542
4.933LeuVal: 4.933 ± 0.573
0.429LeuTrp: 0.429 ± 0.183
1.93LeuTyr: 1.93 ± 0.395
0.0LeuXaa: 0.0 ± 0.0
Met
3.861MetAla: 3.861 ± 0.371
0.214MetCys: 0.214 ± 0.132
0.643MetAsp: 0.643 ± 0.23
1.001MetGlu: 1.001 ± 0.332
0.858MetPhe: 0.858 ± 0.27
0.643MetGly: 0.643 ± 0.187
0.5MetHis: 0.5 ± 0.172
1.859MetIle: 1.859 ± 0.387
2.073MetLys: 2.073 ± 0.406
1.573MetLeu: 1.573 ± 0.369
0.858MetMet: 0.858 ± 0.307
1.787MetAsn: 1.787 ± 0.427
0.715MetPro: 0.715 ± 0.204
0.858MetGln: 0.858 ± 0.231
1.501MetArg: 1.501 ± 0.33
1.287MetSer: 1.287 ± 0.28
2.288MetThr: 2.288 ± 0.442
2.145MetVal: 2.145 ± 0.432
0.214MetTrp: 0.214 ± 0.108
1.001MetTyr: 1.001 ± 0.268
0.0MetXaa: 0.0 ± 0.0
Asn
3.646AsnAla: 3.646 ± 0.467
0.643AsnCys: 0.643 ± 0.277
3.575AsnAsp: 3.575 ± 0.436
3.503AsnGlu: 3.503 ± 0.502
1.287AsnPhe: 1.287 ± 0.274
5.505AsnGly: 5.505 ± 0.861
1.001AsnHis: 1.001 ± 0.218
2.574AsnIle: 2.574 ± 0.399
2.502AsnLys: 2.502 ± 0.357
4.147AsnLeu: 4.147 ± 0.457
1.144AsnMet: 1.144 ± 0.333
3.646AsnAsn: 3.646 ± 0.616
1.43AsnPro: 1.43 ± 0.312
1.644AsnGln: 1.644 ± 0.447
1.93AsnArg: 1.93 ± 0.36
3.861AsnSer: 3.861 ± 0.75
2.359AsnThr: 2.359 ± 0.447
3.932AsnVal: 3.932 ± 0.537
0.643AsnTrp: 0.643 ± 0.168
1.787AsnTyr: 1.787 ± 0.503
0.0AsnXaa: 0.0 ± 0.0
Pro
3.861ProAla: 3.861 ± 0.773
0.357ProCys: 0.357 ± 0.18
1.93ProAsp: 1.93 ± 0.546
2.359ProGlu: 2.359 ± 0.459
1.716ProPhe: 1.716 ± 0.419
1.93ProGly: 1.93 ± 0.43
0.357ProHis: 0.357 ± 0.144
1.287ProIle: 1.287 ± 0.25
1.573ProLys: 1.573 ± 0.375
1.644ProLeu: 1.644 ± 0.403
0.643ProMet: 0.643 ± 0.189
1.501ProAsn: 1.501 ± 0.305
0.786ProPro: 0.786 ± 0.276
2.431ProGln: 2.431 ± 0.841
1.43ProArg: 1.43 ± 0.306
1.43ProSer: 1.43 ± 0.304
1.644ProThr: 1.644 ± 0.409
3.646ProVal: 3.646 ± 0.483
0.357ProTrp: 0.357 ± 0.15
0.929ProTyr: 0.929 ± 0.244
0.0ProXaa: 0.0 ± 0.0
Gln
4.004GlnAla: 4.004 ± 0.739
0.572GlnCys: 0.572 ± 0.199
1.787GlnAsp: 1.787 ± 0.38
2.502GlnGlu: 2.502 ± 0.428
1.501GlnPhe: 1.501 ± 0.301
3.217GlnGly: 3.217 ± 1.479
0.429GlnHis: 0.429 ± 0.154
3.074GlnIle: 3.074 ± 0.859
2.145GlnLys: 2.145 ± 0.381
3.074GlnLeu: 3.074 ± 0.551
0.715GlnMet: 0.715 ± 0.203
1.787GlnAsn: 1.787 ± 0.383
1.43GlnPro: 1.43 ± 0.294
2.86GlnGln: 2.86 ± 0.791
1.787GlnArg: 1.787 ± 0.344
3.074GlnSer: 3.074 ± 0.514
1.358GlnThr: 1.358 ± 0.343
2.145GlnVal: 2.145 ± 0.376
0.429GlnTrp: 0.429 ± 0.188
1.644GlnTyr: 1.644 ± 0.445
0.0GlnXaa: 0.0 ± 0.0
Arg
3.718ArgAla: 3.718 ± 0.553
0.929ArgCys: 0.929 ± 0.337
2.502ArgAsp: 2.502 ± 0.474
3.003ArgGlu: 3.003 ± 0.413
2.431ArgPhe: 2.431 ± 0.44
2.788ArgGly: 2.788 ± 0.465
0.715ArgHis: 0.715 ± 0.273
3.217ArgIle: 3.217 ± 0.525
3.575ArgLys: 3.575 ± 0.616
3.861ArgLeu: 3.861 ± 0.624
0.929ArgMet: 0.929 ± 0.291
2.502ArgAsn: 2.502 ± 0.473
2.073ArgPro: 2.073 ± 0.377
2.359ArgGln: 2.359 ± 0.423
2.86ArgArg: 2.86 ± 0.404
2.431ArgSer: 2.431 ± 0.598
2.073ArgThr: 2.073 ± 0.392
3.289ArgVal: 3.289 ± 0.416
0.5ArgTrp: 0.5 ± 0.186
2.288ArgTyr: 2.288 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
6.363SerAla: 6.363 ± 0.773
0.429SerCys: 0.429 ± 0.221
5.434SerAsp: 5.434 ± 0.57
4.361SerGlu: 4.361 ± 0.632
3.217SerPhe: 3.217 ± 0.574
6.792SerGly: 6.792 ± 0.795
0.643SerHis: 0.643 ± 0.187
4.147SerIle: 4.147 ± 0.482
3.646SerLys: 3.646 ± 0.581
5.577SerLeu: 5.577 ± 0.663
1.43SerMet: 1.43 ± 0.317
3.789SerAsn: 3.789 ± 0.397
2.502SerPro: 2.502 ± 0.485
3.146SerGln: 3.146 ± 0.417
2.502SerArg: 2.502 ± 0.568
6.006SerSer: 6.006 ± 0.999
3.861SerThr: 3.861 ± 0.643
5.005SerVal: 5.005 ± 0.571
0.858SerTrp: 0.858 ± 0.278
2.574SerTyr: 2.574 ± 0.465
0.0SerXaa: 0.0 ± 0.0
Thr
5.434ThrAla: 5.434 ± 0.626
1.072ThrCys: 1.072 ± 0.288
2.931ThrAsp: 2.931 ± 0.4
3.003ThrGlu: 3.003 ± 0.418
1.787ThrPhe: 1.787 ± 0.39
5.791ThrGly: 5.791 ± 0.799
0.643ThrHis: 0.643 ± 0.224
4.361ThrIle: 4.361 ± 0.614
2.431ThrLys: 2.431 ± 0.487
3.289ThrLeu: 3.289 ± 0.482
1.43ThrMet: 1.43 ± 0.292
3.575ThrAsn: 3.575 ± 0.5
2.574ThrPro: 2.574 ± 0.379
2.216ThrGln: 2.216 ± 0.384
2.216ThrArg: 2.216 ± 0.362
4.147ThrSer: 4.147 ± 0.627
3.289ThrThr: 3.289 ± 0.589
3.932ThrVal: 3.932 ± 0.468
0.786ThrTrp: 0.786 ± 0.212
2.288ThrTyr: 2.288 ± 0.436
0.0ThrXaa: 0.0 ± 0.0
Val
4.862ValAla: 4.862 ± 0.633
0.929ValCys: 0.929 ± 0.249
4.576ValAsp: 4.576 ± 0.531
3.503ValGlu: 3.503 ± 0.684
2.931ValPhe: 2.931 ± 0.51
4.862ValGly: 4.862 ± 0.528
0.858ValHis: 0.858 ± 0.236
4.504ValIle: 4.504 ± 0.459
5.148ValLys: 5.148 ± 0.81
4.147ValLeu: 4.147 ± 0.433
2.002ValMet: 2.002 ± 0.346
3.575ValAsn: 3.575 ± 0.591
2.216ValPro: 2.216 ± 0.472
2.645ValGln: 2.645 ± 0.509
4.576ValArg: 4.576 ± 0.597
7.007ValSer: 7.007 ± 0.763
3.432ValThr: 3.432 ± 0.469
5.577ValVal: 5.577 ± 0.758
1.072ValTrp: 1.072 ± 0.195
2.788ValTyr: 2.788 ± 0.488
0.0ValXaa: 0.0 ± 0.0
Trp
0.429TrpAla: 0.429 ± 0.159
0.5TrpCys: 0.5 ± 0.212
0.715TrpAsp: 0.715 ± 0.225
0.429TrpGlu: 0.429 ± 0.159
0.572TrpPhe: 0.572 ± 0.209
0.929TrpGly: 0.929 ± 0.297
0.286TrpHis: 0.286 ± 0.116
1.072TrpIle: 1.072 ± 0.274
0.715TrpLys: 0.715 ± 0.241
1.072TrpLeu: 1.072 ± 0.239
0.286TrpMet: 0.286 ± 0.132
0.286TrpAsn: 0.286 ± 0.108
0.357TrpPro: 0.357 ± 0.136
0.214TrpGln: 0.214 ± 0.152
1.001TrpArg: 1.001 ± 0.279
1.144TrpSer: 1.144 ± 0.338
0.643TrpThr: 0.643 ± 0.188
0.929TrpVal: 0.929 ± 0.242
0.286TrpTrp: 0.286 ± 0.138
0.214TrpTyr: 0.214 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.073TyrAla: 2.073 ± 0.413
0.643TyrCys: 0.643 ± 0.266
3.074TyrAsp: 3.074 ± 0.491
2.788TyrGlu: 2.788 ± 0.48
1.859TyrPhe: 1.859 ± 0.417
3.217TyrGly: 3.217 ± 0.586
0.5TyrHis: 0.5 ± 0.188
2.502TyrIle: 2.502 ± 0.43
2.145TyrLys: 2.145 ± 0.547
1.859TyrLeu: 1.859 ± 0.266
0.929TyrMet: 0.929 ± 0.241
2.502TyrAsn: 2.502 ± 0.418
1.144TyrPro: 1.144 ± 0.305
1.573TyrGln: 1.573 ± 0.238
2.359TyrArg: 2.359 ± 0.441
2.645TyrSer: 2.645 ± 0.574
2.86TyrThr: 2.86 ± 0.401
2.431TyrVal: 2.431 ± 0.394
0.5TyrTrp: 0.5 ± 0.242
1.716TyrTyr: 1.716 ± 0.504
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (13988 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski