Halocella sp. SP3-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halocella; unclassified Halocella

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3701 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S9GYB1|A0A3S9GYB1_9FIRM Carbon starvation protein A OS=Halocella sp. SP3-1 OX=2382161 GN=D7D81_04755 PE=3 SV=1
MM1 pKa = 7.43NDD3 pKa = 2.61IYY5 pKa = 11.12FNYY8 pKa = 10.49LGTQSANILTHH19 pKa = 6.39IALIDD24 pKa = 3.81EE25 pKa = 4.96NGTEE29 pKa = 4.21LDD31 pKa = 4.08YY32 pKa = 11.95VNTIYY37 pKa = 11.05NRR39 pKa = 11.84IPVEE43 pKa = 3.89WEE45 pKa = 3.44IQTDD49 pKa = 3.93GTLTPTVDD57 pKa = 4.49LLFDD61 pKa = 3.76IPGSGDD67 pKa = 3.23SANPNKK73 pKa = 10.16VAGFRR78 pKa = 11.84MFDD81 pKa = 3.38AEE83 pKa = 4.48TDD85 pKa = 3.49GTNYY89 pKa = 10.05GGKK92 pKa = 10.04DD93 pKa = 3.33IQIKK97 pKa = 10.34EE98 pKa = 4.26YY99 pKa = 9.89VTDD102 pKa = 4.01GQYY105 pKa = 9.8TLPQDD110 pKa = 3.96KK111 pKa = 10.69IFIHH115 pKa = 6.4FNNVDD120 pKa = 3.43IEE122 pKa = 4.43PIII125 pKa = 4.01

Molecular weight: 14.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S9GX93|A0A3S9GX93_9FIRM Uncharacterized protein OS=Halocella sp. SP3-1 OX=2382161 GN=D7D81_02105 PE=4 SV=1
MM1 pKa = 7.42PRR3 pKa = 11.84GRR5 pKa = 11.84GKK7 pKa = 10.71SCFFCANKK15 pKa = 10.4DD16 pKa = 3.48KK17 pKa = 11.26EE18 pKa = 4.27IDD20 pKa = 3.88YY21 pKa = 11.13KK22 pKa = 10.54DD23 pKa = 3.79LRR25 pKa = 11.84TLQRR29 pKa = 11.84FVTDD33 pKa = 3.15RR34 pKa = 11.84GKK36 pKa = 10.11IVPRR40 pKa = 11.84RR41 pKa = 11.84ITGTCAKK48 pKa = 9.4HH49 pKa = 5.36QRR51 pKa = 11.84ALTRR55 pKa = 11.84QIKK58 pKa = 9.25RR59 pKa = 11.84ARR61 pKa = 11.84AIALLPYY68 pKa = 10.28VKK70 pKa = 10.29EE71 pKa = 4.05

Molecular weight: 8.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3701

0

3701

1139644

28

6823

307.9

34.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.444 ± 0.048

0.836 ± 0.016

5.581 ± 0.034

7.389 ± 0.047

4.265 ± 0.035

7.005 ± 0.041

1.472 ± 0.015

9.378 ± 0.042

7.628 ± 0.055

10.027 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.023

5.346 ± 0.046

3.179 ± 0.025

3.027 ± 0.019

4.038 ± 0.031

5.87 ± 0.038

4.645 ± 0.039

6.55 ± 0.034

0.865 ± 0.015

3.963 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski