Amino acid dipepetide frequency for Halocella sp. SP3-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.555AlaAla: 5.555 ± 0.103
0.592AlaCys: 0.592 ± 0.027
3.735AlaAsp: 3.735 ± 0.075
4.93AlaGlu: 4.93 ± 0.078
2.42AlaPhe: 2.42 ± 0.05
5.572AlaGly: 5.572 ± 0.114
0.895AlaHis: 0.895 ± 0.032
5.239AlaIle: 5.239 ± 0.088
3.824AlaLys: 3.824 ± 0.055
6.232AlaLeu: 6.232 ± 0.079
1.594AlaMet: 1.594 ± 0.042
2.738AlaAsn: 2.738 ± 0.049
1.45AlaPro: 1.45 ± 0.04
1.823AlaGln: 1.823 ± 0.042
3.205AlaArg: 3.205 ± 0.052
3.602AlaSer: 3.602 ± 0.073
2.667AlaThr: 2.667 ± 0.056
5.654AlaVal: 5.654 ± 0.097
0.474AlaTrp: 0.474 ± 0.023
2.237AlaTyr: 2.237 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.422CysAla: 0.422 ± 0.027
0.117CysCys: 0.117 ± 0.011
0.471CysAsp: 0.471 ± 0.021
0.449CysGlu: 0.449 ± 0.02
0.347CysPhe: 0.347 ± 0.019
0.765CysGly: 0.765 ± 0.028
0.191CysHis: 0.191 ± 0.013
0.635CysIle: 0.635 ± 0.025
0.551CysLys: 0.551 ± 0.022
0.859CysLeu: 0.859 ± 0.027
0.165CysMet: 0.165 ± 0.011
0.433CysAsn: 0.433 ± 0.02
0.412CysPro: 0.412 ± 0.021
0.398CysGln: 0.398 ± 0.02
0.373CysArg: 0.373 ± 0.02
0.565CysSer: 0.565 ± 0.024
0.38CysThr: 0.38 ± 0.019
0.426CysVal: 0.426 ± 0.019
0.089CysTrp: 0.089 ± 0.008
0.31CysTyr: 0.31 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
2.444AspAla: 2.444 ± 0.055
0.499AspCys: 0.499 ± 0.02
2.972AspAsp: 2.972 ± 0.067
4.239AspGlu: 4.239 ± 0.065
2.819AspPhe: 2.819 ± 0.049
3.449AspGly: 3.449 ± 0.065
0.685AspHis: 0.685 ± 0.025
5.802AspIle: 5.802 ± 0.085
4.653AspLys: 4.653 ± 0.077
5.833AspLeu: 5.833 ± 0.071
1.364AspMet: 1.364 ± 0.038
3.261AspAsn: 3.261 ± 0.057
1.668AspPro: 1.668 ± 0.039
1.532AspGln: 1.532 ± 0.04
2.505AspArg: 2.505 ± 0.049
3.096AspSer: 3.096 ± 0.059
2.293AspThr: 2.293 ± 0.054
3.111AspVal: 3.111 ± 0.053
0.611AspTrp: 0.611 ± 0.023
2.975AspTyr: 2.975 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
4.406GluAla: 4.406 ± 0.073
0.414GluCys: 0.414 ± 0.019
4.034GluAsp: 4.034 ± 0.066
7.036GluGlu: 7.036 ± 0.109
2.673GluPhe: 2.673 ± 0.05
4.505GluGly: 4.505 ± 0.066
1.037GluHis: 1.037 ± 0.03
7.524GluIle: 7.524 ± 0.084
6.726GluLys: 6.726 ± 0.091
7.981GluLeu: 7.981 ± 0.076
2.066GluMet: 2.066 ± 0.047
4.001GluAsn: 4.001 ± 0.062
1.711GluPro: 1.711 ± 0.057
2.224GluGln: 2.224 ± 0.049
3.121GluArg: 3.121 ± 0.065
3.053GluSer: 3.053 ± 0.053
2.974GluThr: 2.974 ± 0.053
4.96GluVal: 4.96 ± 0.07
0.562GluTrp: 0.562 ± 0.023
2.88GluTyr: 2.88 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
2.73PheAla: 2.73 ± 0.052
0.386PheCys: 0.386 ± 0.019
2.388PheAsp: 2.388 ± 0.052
2.341PheGlu: 2.341 ± 0.055
2.197PhePhe: 2.197 ± 0.053
2.696PheGly: 2.696 ± 0.058
0.617PheHis: 0.617 ± 0.024
4.162PheIle: 4.162 ± 0.085
3.195PheLys: 3.195 ± 0.053
4.289PheLeu: 4.289 ± 0.086
1.098PheMet: 1.098 ± 0.032
2.503PheAsn: 2.503 ± 0.047
1.385PhePro: 1.385 ± 0.04
1.157PheGln: 1.157 ± 0.034
1.48PheArg: 1.48 ± 0.036
3.201PheSer: 3.201 ± 0.06
2.294PheThr: 2.294 ± 0.051
2.503PheVal: 2.503 ± 0.056
0.38PheTrp: 0.38 ± 0.021
1.651PheTyr: 1.651 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.348GlyAla: 4.348 ± 0.102
0.669GlyCys: 0.669 ± 0.026
3.556GlyAsp: 3.556 ± 0.064
4.876GlyGlu: 4.876 ± 0.075
3.291GlyPhe: 3.291 ± 0.059
4.74GlyGly: 4.74 ± 0.083
1.102GlyHis: 1.102 ± 0.034
7.24GlyIle: 7.24 ± 0.073
5.175GlyLys: 5.175 ± 0.066
7.303GlyLeu: 7.303 ± 0.081
1.951GlyMet: 1.951 ± 0.05
3.072GlyAsn: 3.072 ± 0.088
1.554GlyPro: 1.554 ± 0.039
2.145GlyGln: 2.145 ± 0.055
2.852GlyArg: 2.852 ± 0.049
4.055GlySer: 4.055 ± 0.08
3.289GlyThr: 3.289 ± 0.072
5.124GlyVal: 5.124 ± 0.075
0.67GlyTrp: 0.67 ± 0.029
3.042GlyTyr: 3.042 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
0.744HisAla: 0.744 ± 0.027
0.181HisCys: 0.181 ± 0.013
0.749HisAsp: 0.749 ± 0.026
0.871HisGlu: 0.871 ± 0.028
0.72HisPhe: 0.72 ± 0.024
1.094HisGly: 1.094 ± 0.032
0.371HisHis: 0.371 ± 0.02
1.214HisIle: 1.214 ± 0.037
0.925HisLys: 0.925 ± 0.03
1.522HisLeu: 1.522 ± 0.036
0.276HisMet: 0.276 ± 0.017
0.843HisAsn: 0.843 ± 0.029
0.741HisPro: 0.741 ± 0.026
0.62HisGln: 0.62 ± 0.025
0.632HisArg: 0.632 ± 0.025
0.884HisSer: 0.884 ± 0.029
0.687HisThr: 0.687 ± 0.024
0.772HisVal: 0.772 ± 0.026
0.165HisTrp: 0.165 ± 0.013
0.708HisTyr: 0.708 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.477IleAla: 6.477 ± 0.082
0.831IleCys: 0.831 ± 0.029
5.678IleAsp: 5.678 ± 0.076
6.254IleGlu: 6.254 ± 0.089
4.01IlePhe: 4.01 ± 0.071
6.052IleGly: 6.052 ± 0.083
1.114IleHis: 1.114 ± 0.03
9.464IleIle: 9.464 ± 0.124
7.798IleLys: 7.798 ± 0.093
8.547IleLeu: 8.547 ± 0.117
2.266IleMet: 2.266 ± 0.051
5.665IleAsn: 5.665 ± 0.092
3.619IlePro: 3.619 ± 0.067
2.078IleGln: 2.078 ± 0.051
3.314IleArg: 3.314 ± 0.061
6.359IleSer: 6.359 ± 0.088
4.651IleThr: 4.651 ± 0.073
5.631IleVal: 5.631 ± 0.076
0.655IleTrp: 0.655 ± 0.027
3.321IleTyr: 3.321 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
4.617LysAla: 4.617 ± 0.069
0.454LysCys: 0.454 ± 0.02
4.327LysAsp: 4.327 ± 0.074
7.482LysGlu: 7.482 ± 0.104
2.278LysPhe: 2.278 ± 0.055
5.049LysGly: 5.049 ± 0.069
1.013LysHis: 1.013 ± 0.032
7.11LysIle: 7.11 ± 0.086
7.099LysLys: 7.099 ± 0.108
6.883LysLeu: 6.883 ± 0.096
2.076LysMet: 2.076 ± 0.046
4.337LysAsn: 4.337 ± 0.072
2.123LysPro: 2.123 ± 0.048
2.3LysGln: 2.3 ± 0.046
3.139LysArg: 3.139 ± 0.068
3.663LysSer: 3.663 ± 0.061
3.629LysThr: 3.629 ± 0.061
4.732LysVal: 4.732 ± 0.062
0.623LysTrp: 0.623 ± 0.026
3.346LysTyr: 3.346 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
7.555LeuAla: 7.555 ± 0.081
0.778LeuCys: 0.778 ± 0.027
5.481LeuAsp: 5.481 ± 0.071
6.884LeuGlu: 6.884 ± 0.093
4.139LeuPhe: 4.139 ± 0.083
6.776LeuGly: 6.776 ± 0.097
1.301LeuHis: 1.301 ± 0.035
9.335LeuIle: 9.335 ± 0.125
7.824LeuLys: 7.824 ± 0.091
10.256LeuLeu: 10.256 ± 0.131
2.443LeuMet: 2.443 ± 0.05
5.403LeuAsn: 5.403 ± 0.089
3.619LeuPro: 3.619 ± 0.067
2.79LeuGln: 2.79 ± 0.055
3.632LeuArg: 3.632 ± 0.067
6.912LeuSer: 6.912 ± 0.09
5.066LeuThr: 5.066 ± 0.078
6.115LeuVal: 6.115 ± 0.084
0.735LeuTrp: 0.735 ± 0.029
3.221LeuTyr: 3.221 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
2.041MetAla: 2.041 ± 0.045
0.156MetCys: 0.156 ± 0.012
1.357MetAsp: 1.357 ± 0.036
1.785MetGlu: 1.785 ± 0.042
0.903MetPhe: 0.903 ± 0.031
2.084MetGly: 2.084 ± 0.045
0.298MetHis: 0.298 ± 0.014
2.255MetIle: 2.255 ± 0.041
2.067MetLys: 2.067 ± 0.048
2.204MetLeu: 2.204 ± 0.051
0.703MetMet: 0.703 ± 0.025
1.295MetAsn: 1.295 ± 0.033
0.827MetPro: 0.827 ± 0.026
0.648MetGln: 0.648 ± 0.025
0.907MetArg: 0.907 ± 0.032
1.5MetSer: 1.5 ± 0.033
1.266MetThr: 1.266 ± 0.033
1.823MetVal: 1.823 ± 0.043
0.162MetTrp: 0.162 ± 0.013
0.638MetTyr: 0.638 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.349AsnAla: 2.349 ± 0.06
0.519AsnCys: 0.519 ± 0.025
2.702AsnAsp: 2.702 ± 0.054
3.389AsnGlu: 3.389 ± 0.059
2.266AsnPhe: 2.266 ± 0.046
3.146AsnGly: 3.146 ± 0.068
0.799AsnHis: 0.799 ± 0.024
5.382AsnIle: 5.382 ± 0.088
4.721AsnLys: 4.721 ± 0.082
5.205AsnLeu: 5.205 ± 0.077
1.284AsnMet: 1.284 ± 0.035
3.86AsnAsn: 3.86 ± 0.098
2.263AsnPro: 2.263 ± 0.049
1.821AsnGln: 1.821 ± 0.042
2.145AsnArg: 2.145 ± 0.045
3.169AsnSer: 3.169 ± 0.069
2.451AsnThr: 2.451 ± 0.057
2.801AsnVal: 2.801 ± 0.072
0.584AsnTrp: 0.584 ± 0.025
2.603AsnTyr: 2.603 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
2.351ProAla: 2.351 ± 0.047
0.254ProCys: 0.254 ± 0.016
2.112ProAsp: 2.112 ± 0.044
2.974ProGlu: 2.974 ± 0.064
1.488ProPhe: 1.488 ± 0.04
2.545ProGly: 2.545 ± 0.054
0.576ProHis: 0.576 ± 0.026
2.403ProIle: 2.403 ± 0.051
1.445ProLys: 1.445 ± 0.035
3.163ProLeu: 3.163 ± 0.055
0.665ProMet: 0.665 ± 0.029
1.155ProAsn: 1.155 ± 0.034
0.841ProPro: 0.841 ± 0.032
0.982ProGln: 0.982 ± 0.03
1.093ProArg: 1.093 ± 0.032
1.593ProSer: 1.593 ± 0.04
1.54ProThr: 1.54 ± 0.034
2.894ProVal: 2.894 ± 0.049
0.337ProTrp: 0.337 ± 0.018
1.381ProTyr: 1.381 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.216GlnAla: 2.216 ± 0.047
0.192GlnCys: 0.192 ± 0.013
1.591GlnAsp: 1.591 ± 0.039
2.9GlnGlu: 2.9 ± 0.06
0.982GlnPhe: 0.982 ± 0.031
2.355GlnGly: 2.355 ± 0.049
0.44GlnHis: 0.44 ± 0.022
2.36GlnIle: 2.36 ± 0.053
2.341GlnLys: 2.341 ± 0.053
3.183GlnLeu: 3.183 ± 0.055
0.738GlnMet: 0.738 ± 0.026
1.429GlnAsn: 1.429 ± 0.039
0.813GlnPro: 0.813 ± 0.028
1.31GlnGln: 1.31 ± 0.038
1.324GlnArg: 1.324 ± 0.033
1.429GlnSer: 1.429 ± 0.034
1.285GlnThr: 1.285 ± 0.039
2.038GlnVal: 2.038 ± 0.048
0.248GlnTrp: 0.248 ± 0.016
1.098GlnTyr: 1.098 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
2.285ArgAla: 2.285 ± 0.054
0.353ArgCys: 0.353 ± 0.018
2.527ArgAsp: 2.527 ± 0.046
3.523ArgGlu: 3.523 ± 0.064
1.738ArgPhe: 1.738 ± 0.044
2.739ArgGly: 2.739 ± 0.047
0.63ArgHis: 0.63 ± 0.025
3.601ArgIle: 3.601 ± 0.052
3.143ArgLys: 3.143 ± 0.065
4.074ArgLeu: 4.074 ± 0.07
1.021ArgMet: 1.021 ± 0.027
1.937ArgAsn: 1.937 ± 0.041
1.181ArgPro: 1.181 ± 0.032
1.386ArgGln: 1.386 ± 0.032
1.725ArgArg: 1.725 ± 0.049
1.868ArgSer: 1.868 ± 0.044
1.686ArgThr: 1.686 ± 0.044
2.951ArgVal: 2.951 ± 0.053
0.347ArgTrp: 0.347 ± 0.018
1.666ArgTyr: 1.666 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
3.358SerAla: 3.358 ± 0.061
0.558SerCys: 0.558 ± 0.023
2.927SerAsp: 2.927 ± 0.052
3.419SerGlu: 3.419 ± 0.057
3.054SerPhe: 3.054 ± 0.059
4.515SerGly: 4.515 ± 0.085
0.992SerHis: 0.992 ± 0.031
5.472SerIle: 5.472 ± 0.083
3.99SerLys: 3.99 ± 0.055
6.383SerLeu: 6.383 ± 0.089
1.318SerMet: 1.318 ± 0.036
3.034SerAsn: 3.034 ± 0.056
1.903SerPro: 1.903 ± 0.041
1.84SerGln: 1.84 ± 0.041
2.671SerArg: 2.671 ± 0.049
4.039SerSer: 4.039 ± 0.07
2.669SerThr: 2.669 ± 0.061
3.561SerVal: 3.561 ± 0.073
0.61SerTrp: 0.61 ± 0.025
2.382SerTyr: 2.382 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
3.949ThrAla: 3.949 ± 0.098
0.303ThrCys: 0.303 ± 0.016
2.662ThrAsp: 2.662 ± 0.058
2.982ThrGlu: 2.982 ± 0.062
1.805ThrPhe: 1.805 ± 0.039
4.331ThrGly: 4.331 ± 0.087
0.726ThrHis: 0.726 ± 0.027
4.293ThrIle: 4.293 ± 0.07
2.555ThrLys: 2.555 ± 0.045
4.348ThrLeu: 4.348 ± 0.066
1.07ThrMet: 1.07 ± 0.035
2.111ThrAsn: 2.111 ± 0.049
1.91ThrPro: 1.91 ± 0.046
0.992ThrGln: 0.992 ± 0.031
1.816ThrArg: 1.816 ± 0.044
2.624ThrSer: 2.624 ± 0.057
2.466ThrThr: 2.466 ± 0.069
3.573ThrVal: 3.573 ± 0.059
0.369ThrTrp: 0.369 ± 0.019
1.572ThrTyr: 1.572 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
4.221ValAla: 4.221 ± 0.081
0.606ValCys: 0.606 ± 0.026
3.886ValAsp: 3.886 ± 0.061
4.687ValGlu: 4.687 ± 0.07
3.206ValPhe: 3.206 ± 0.057
4.346ValGly: 4.346 ± 0.083
0.919ValHis: 0.919 ± 0.028
6.26ValIle: 6.26 ± 0.078
4.994ValLys: 4.994 ± 0.068
6.6ValLeu: 6.6 ± 0.085
1.79ValMet: 1.79 ± 0.038
3.495ValAsn: 3.495 ± 0.081
2.043ValPro: 2.043 ± 0.045
1.589ValGln: 1.589 ± 0.038
2.354ValArg: 2.354 ± 0.047
4.216ValSer: 4.216 ± 0.068
2.925ValThr: 2.925 ± 0.059
4.515ValVal: 4.515 ± 0.071
0.49ValTrp: 0.49 ± 0.024
2.355ValTyr: 2.355 ± 0.045
0.0ValXaa: 0.0 ± 0.0
Trp
0.475TrpAla: 0.475 ± 0.022
0.078TrpCys: 0.078 ± 0.008
0.539TrpAsp: 0.539 ± 0.021
0.624TrpGlu: 0.624 ± 0.025
0.384TrpPhe: 0.384 ± 0.02
0.698TrpGly: 0.698 ± 0.026
0.148TrpHis: 0.148 ± 0.011
0.668TrpIle: 0.668 ± 0.027
0.541TrpLys: 0.541 ± 0.024
0.896TrpLeu: 0.896 ± 0.033
0.227TrpMet: 0.227 ± 0.013
0.428TrpAsn: 0.428 ± 0.023
0.306TrpPro: 0.306 ± 0.017
0.467TrpGln: 0.467 ± 0.021
0.347TrpArg: 0.347 ± 0.019
0.469TrpSer: 0.469 ± 0.019
0.407TrpThr: 0.407 ± 0.02
0.509TrpVal: 0.509 ± 0.023
0.118TrpTrp: 0.118 ± 0.012
0.325TrpTyr: 0.325 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.897TyrAla: 1.897 ± 0.041
0.417TyrCys: 0.417 ± 0.018
2.115TyrAsp: 2.115 ± 0.046
2.245TyrGlu: 2.245 ± 0.051
1.931TyrPhe: 1.931 ± 0.045
2.596TyrGly: 2.596 ± 0.044
0.863TyrHis: 0.863 ± 0.029
3.364TyrIle: 3.364 ± 0.057
2.713TyrLys: 2.713 ± 0.059
4.505TyrLeu: 4.505 ± 0.069
0.806TyrMet: 0.806 ± 0.028
2.456TyrAsn: 2.456 ± 0.052
1.424TyrPro: 1.424 ± 0.04
2.171TyrGln: 2.171 ± 0.047
1.744TyrArg: 1.744 ± 0.041
2.401TyrSer: 2.401 ± 0.046
1.831TyrThr: 1.831 ± 0.042
1.806TyrVal: 1.806 ± 0.038
0.425TyrTrp: 0.425 ± 0.02
1.917TyrTyr: 1.917 ± 0.048
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3701 proteins (1139644 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski