Lactobacillus phage 521B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Tybeckvirus; Lactobacillus virus 521B

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5FEG1|A0A4Y5FEG1_9CAUD Transposase OS=Lactobacillus phage 521B OX=2510942 GN=B521_0072 PE=3 SV=1
MM1 pKa = 7.46TNQSIKK7 pKa = 10.66AYY9 pKa = 9.68FYY11 pKa = 11.16NGFNEE16 pKa = 4.36PEE18 pKa = 4.82LIATDD23 pKa = 3.57IEE25 pKa = 4.32EE26 pKa = 4.36LKK28 pKa = 10.96HH29 pKa = 6.88VIIYY33 pKa = 10.4DD34 pKa = 3.6NYY36 pKa = 10.17IGDD39 pKa = 3.71VSKK42 pKa = 11.43DD43 pKa = 3.53LLDD46 pKa = 5.87DD47 pKa = 4.25INSCSDD53 pKa = 3.5LDD55 pKa = 4.01DD56 pKa = 4.84LKK58 pKa = 11.62EE59 pKa = 3.78EE60 pKa = 4.28LAYY63 pKa = 10.49FDD65 pKa = 5.38IFINPDD71 pKa = 3.04KK72 pKa = 11.05QDD74 pKa = 4.13FIEE77 pKa = 4.96ANKK80 pKa = 10.65AWFEE84 pKa = 3.96PDD86 pKa = 2.97GLEE89 pKa = 4.9NYY91 pKa = 10.11LEE93 pKa = 4.13NWW95 pKa = 3.31

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5FED1|A0A4Y5FED1_9CAUD Uncharacterized protein OS=Lactobacillus phage 521B OX=2510942 GN=B521_0051 PE=4 SV=1
MM1 pKa = 6.67LTSYY5 pKa = 10.46KK6 pKa = 10.45VEE8 pKa = 4.25INPTSQQAWLIDD20 pKa = 3.46RR21 pKa = 11.84HH22 pKa = 6.54ISASRR27 pKa = 11.84WAYY30 pKa = 11.43NLFLDD35 pKa = 4.63INKK38 pKa = 8.6QRR40 pKa = 11.84YY41 pKa = 7.37EE42 pKa = 4.05DD43 pKa = 4.44GYY45 pKa = 10.61HH46 pKa = 6.43YY47 pKa = 10.37MSAYY51 pKa = 9.78EE52 pKa = 3.72FSKK55 pKa = 10.21WFNHH59 pKa = 7.38DD60 pKa = 3.77YY61 pKa = 11.56LDD63 pKa = 5.43FNPDD67 pKa = 3.76DD68 pKa = 3.54NWIKK72 pKa = 10.44QLYY75 pKa = 9.48AKK77 pKa = 8.63STKK80 pKa = 9.42QAFIDD85 pKa = 4.09ADD87 pKa = 3.27NSMKK91 pKa = 10.55RR92 pKa = 11.84FFKK95 pKa = 10.47KK96 pKa = 9.5QSRR99 pKa = 11.84YY100 pKa = 7.51PHH102 pKa = 5.8FRR104 pKa = 11.84SFKK107 pKa = 8.39RR108 pKa = 11.84GKK110 pKa = 9.84GSFYY114 pKa = 10.57FVKK117 pKa = 10.61NSNKK121 pKa = 10.33DD122 pKa = 3.17IIRR125 pKa = 11.84VEE127 pKa = 3.71RR128 pKa = 11.84HH129 pKa = 5.95RR130 pKa = 11.84IKK132 pKa = 10.57LPKK135 pKa = 9.72IGWVRR140 pKa = 11.84LKK142 pKa = 10.61EE143 pKa = 3.92KK144 pKa = 10.73GYY146 pKa = 10.14IPTDD150 pKa = 3.37AEE152 pKa = 4.16HH153 pKa = 6.73FVIKK157 pKa = 10.12QGRR160 pKa = 11.84VKK162 pKa = 10.32KK163 pKa = 10.04HH164 pKa = 5.57AGRR167 pKa = 11.84YY168 pKa = 8.77YY169 pKa = 9.42LTCLVEE175 pKa = 3.87QADD178 pKa = 3.69IQFAKK183 pKa = 10.9LNGDD187 pKa = 4.18PIGIDD192 pKa = 3.26LGIKK196 pKa = 9.74EE197 pKa = 4.48FAILSNGTIFRR208 pKa = 11.84NKK210 pKa = 9.62NKK212 pKa = 9.8SSRR215 pKa = 11.84VRR217 pKa = 11.84GMRR220 pKa = 11.84KK221 pKa = 9.14RR222 pKa = 11.84LRR224 pKa = 11.84RR225 pKa = 11.84LQRR228 pKa = 11.84KK229 pKa = 6.98WSRR232 pKa = 11.84QYY234 pKa = 11.38LAFKK238 pKa = 10.39ARR240 pKa = 11.84KK241 pKa = 8.33QKK243 pKa = 10.47EE244 pKa = 4.27GSSATDD250 pKa = 3.53LNLAKK255 pKa = 9.89TKK257 pKa = 11.05LKK259 pKa = 9.07MQRR262 pKa = 11.84LYY264 pKa = 10.99QRR266 pKa = 11.84LQNVQTDD273 pKa = 3.99YY274 pKa = 10.83QKK276 pKa = 11.22KK277 pKa = 9.9IISQVVRR284 pKa = 11.84TKK286 pKa = 9.7PQWVALEE293 pKa = 4.26DD294 pKa = 3.76LNIRR298 pKa = 11.84GMAKK302 pKa = 10.04NKK304 pKa = 9.85HH305 pKa = 4.65LAKK308 pKa = 10.59AIMQQGFYY316 pKa = 10.97AFRR319 pKa = 11.84EE320 pKa = 3.99KK321 pKa = 10.83LINKK325 pKa = 7.38CQQLGIPVHH334 pKa = 6.46LINRR338 pKa = 11.84FEE340 pKa = 4.14PTSKK344 pKa = 10.43CCHH347 pKa = 5.6NCGARR352 pKa = 11.84KK353 pKa = 10.04VNLSLSDD360 pKa = 4.54RR361 pKa = 11.84IYY363 pKa = 10.32HH364 pKa = 6.69CDD366 pKa = 3.02VCGYY370 pKa = 9.37TEE372 pKa = 5.06DD373 pKa = 4.67RR374 pKa = 11.84DD375 pKa = 3.81INASLNIRR383 pKa = 11.84DD384 pKa = 3.89TEE386 pKa = 4.14NFEE389 pKa = 4.16LAYY392 pKa = 10.5

Molecular weight: 46.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

188

0

188

40347

43

1169

214.6

24.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.838 ± 0.209

0.617 ± 0.059

7.264 ± 0.175

5.929 ± 0.25

4.008 ± 0.122

5.547 ± 0.235

1.376 ± 0.089

7.683 ± 0.194

8.685 ± 0.282

8.291 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.077

7.773 ± 0.184

2.922 ± 0.14

3.222 ± 0.154

3.066 ± 0.149

8.219 ± 0.313

5.938 ± 0.243

5.805 ± 0.135

1.068 ± 0.073

5.517 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski