Amino acid dipepetide frequency for Lactobacillus phage 521B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.289AlaAla: 1.289 ± 0.265
0.198AlaCys: 0.198 ± 0.07
3.396AlaAsp: 3.396 ± 0.365
2.503AlaGlu: 2.503 ± 0.317
1.983AlaPhe: 1.983 ± 0.245
2.677AlaGly: 2.677 ± 0.485
0.595AlaHis: 0.595 ± 0.144
3.842AlaIle: 3.842 ± 0.329
4.709AlaLys: 4.709 ± 0.42
3.544AlaLeu: 3.544 ± 0.316
1.363AlaMet: 1.363 ± 0.212
4.065AlaAsn: 4.065 ± 0.331
1.388AlaPro: 1.388 ± 0.228
1.462AlaGln: 1.462 ± 0.22
1.462AlaArg: 1.462 ± 0.311
4.263AlaSer: 4.263 ± 0.367
3.544AlaThr: 3.544 ± 0.399
2.404AlaVal: 2.404 ± 0.273
0.744AlaTrp: 0.744 ± 0.129
2.949AlaTyr: 2.949 ± 0.258
0.0AlaXaa: 0.0 ± 0.0
Cys
0.149CysAla: 0.149 ± 0.062
0.099CysCys: 0.099 ± 0.049
0.57CysAsp: 0.57 ± 0.121
0.273CysGlu: 0.273 ± 0.087
0.297CysPhe: 0.297 ± 0.095
0.545CysGly: 0.545 ± 0.126
0.074CysHis: 0.074 ± 0.04
0.421CysIle: 0.421 ± 0.086
0.496CysLys: 0.496 ± 0.139
0.669CysLeu: 0.669 ± 0.122
0.124CysMet: 0.124 ± 0.056
0.273CysAsn: 0.273 ± 0.084
0.273CysPro: 0.273 ± 0.079
0.248CysGln: 0.248 ± 0.071
0.173CysArg: 0.173 ± 0.07
0.397CysSer: 0.397 ± 0.1
0.322CysThr: 0.322 ± 0.102
0.297CysVal: 0.297 ± 0.087
0.099CysTrp: 0.099 ± 0.044
0.372CysTyr: 0.372 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
3.272AspAla: 3.272 ± 0.29
0.322AspCys: 0.322 ± 0.095
4.486AspAsp: 4.486 ± 0.404
3.842AspGlu: 3.842 ± 0.29
3.594AspPhe: 3.594 ± 0.364
3.495AspGly: 3.495 ± 0.307
0.744AspHis: 0.744 ± 0.117
7.213AspIle: 7.213 ± 0.435
7.684AspLys: 7.684 ± 0.5
6.667AspLeu: 6.667 ± 0.535
2.008AspMet: 2.008 ± 0.202
5.552AspAsn: 5.552 ± 0.425
1.785AspPro: 1.785 ± 0.217
1.338AspGln: 1.338 ± 0.206
2.107AspArg: 2.107 ± 0.245
6.444AspSer: 6.444 ± 0.47
3.941AspThr: 3.941 ± 0.306
3.346AspVal: 3.346 ± 0.301
0.669AspTrp: 0.669 ± 0.117
4.139AspTyr: 4.139 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
3.049GluAla: 3.049 ± 0.286
0.347GluCys: 0.347 ± 0.084
5.428GluAsp: 5.428 ± 0.41
5.031GluGlu: 5.031 ± 0.447
2.404GluPhe: 2.404 ± 0.243
2.826GluGly: 2.826 ± 0.253
0.843GluHis: 0.843 ± 0.159
4.412GluIle: 4.412 ± 0.325
5.973GluLys: 5.973 ± 0.422
5.602GluLeu: 5.602 ± 0.468
1.264GluMet: 1.264 ± 0.174
4.337GluAsn: 4.337 ± 0.435
1.066GluPro: 1.066 ± 0.184
2.082GluGln: 2.082 ± 0.246
1.561GluArg: 1.561 ± 0.193
3.42GluSer: 3.42 ± 0.282
2.751GluThr: 2.751 ± 0.3
3.693GluVal: 3.693 ± 0.373
0.496GluTrp: 0.496 ± 0.103
2.677GluTyr: 2.677 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
1.561PheAla: 1.561 ± 0.242
0.223PheCys: 0.223 ± 0.078
2.974PheAsp: 2.974 ± 0.261
1.983PheGlu: 1.983 ± 0.225
1.214PhePhe: 1.214 ± 0.206
2.057PheGly: 2.057 ± 0.231
0.496PheHis: 0.496 ± 0.122
2.949PheIle: 2.949 ± 0.288
3.321PheLys: 3.321 ± 0.322
3.272PheLeu: 3.272 ± 0.288
0.967PheMet: 0.967 ± 0.153
3.891PheAsn: 3.891 ± 0.302
1.388PhePro: 1.388 ± 0.178
1.016PheGln: 1.016 ± 0.201
1.413PheArg: 1.413 ± 0.178
4.09PheSer: 4.09 ± 0.298
2.181PheThr: 2.181 ± 0.249
2.454PheVal: 2.454 ± 0.33
0.347PheTrp: 0.347 ± 0.09
2.28PheTyr: 2.28 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
2.231GlyAla: 2.231 ± 0.362
0.297GlyCys: 0.297 ± 0.089
3.817GlyAsp: 3.817 ± 0.361
2.379GlyGlu: 2.379 ± 0.222
2.156GlyPhe: 2.156 ± 0.208
2.925GlyGly: 2.925 ± 0.586
0.843GlyHis: 0.843 ± 0.145
6.072GlyIle: 6.072 ± 0.405
5.007GlyLys: 5.007 ± 0.413
4.164GlyLeu: 4.164 ± 0.344
1.314GlyMet: 1.314 ± 0.16
4.511GlyAsn: 4.511 ± 0.39
0.421GlyPro: 0.421 ± 0.106
1.066GlyGln: 1.066 ± 0.175
1.785GlyArg: 1.785 ± 0.234
4.412GlySer: 4.412 ± 0.404
3.346GlyThr: 3.346 ± 0.367
3.867GlyVal: 3.867 ± 0.368
0.694GlyTrp: 0.694 ± 0.153
4.164GlyTyr: 4.164 ± 0.32
0.0GlyXaa: 0.0 ± 0.0
His
0.446HisAla: 0.446 ± 0.099
0.173HisCys: 0.173 ± 0.06
0.843HisAsp: 0.843 ± 0.164
0.471HisGlu: 0.471 ± 0.133
0.644HisPhe: 0.644 ± 0.102
0.843HisGly: 0.843 ± 0.202
0.52HisHis: 0.52 ± 0.115
1.066HisIle: 1.066 ± 0.165
1.041HisLys: 1.041 ± 0.17
1.289HisLeu: 1.289 ± 0.179
0.471HisMet: 0.471 ± 0.113
0.967HisAsn: 0.967 ± 0.133
0.57HisPro: 0.57 ± 0.116
0.471HisGln: 0.471 ± 0.103
0.57HisArg: 0.57 ± 0.114
0.793HisSer: 0.793 ± 0.14
0.744HisThr: 0.744 ± 0.142
0.818HisVal: 0.818 ± 0.166
0.198HisTrp: 0.198 ± 0.072
0.818HisTyr: 0.818 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
3.767IleAla: 3.767 ± 0.298
0.397IleCys: 0.397 ± 0.116
6.196IleAsp: 6.196 ± 0.418
4.288IleGlu: 4.288 ± 0.304
2.28IlePhe: 2.28 ± 0.261
4.412IleGly: 4.412 ± 0.294
0.917IleHis: 0.917 ± 0.148
5.155IleIle: 5.155 ± 0.38
8.154IleLys: 8.154 ± 0.402
5.478IleLeu: 5.478 ± 0.363
1.586IleMet: 1.586 ± 0.176
6.94IleAsn: 6.94 ± 0.429
2.949IlePro: 2.949 ± 0.238
2.156IleGln: 2.156 ± 0.238
2.206IleArg: 2.206 ± 0.255
7.064IleSer: 7.064 ± 0.545
5.18IleThr: 5.18 ± 0.431
3.817IleVal: 3.817 ± 0.285
0.496IleTrp: 0.496 ± 0.12
3.396IleTyr: 3.396 ± 0.272
0.0IleXaa: 0.0 ± 0.0
Lys
4.536LysAla: 4.536 ± 0.364
0.595LysCys: 0.595 ± 0.122
7.089LysAsp: 7.089 ± 0.507
8.154LysGlu: 8.154 ± 0.663
3.197LysPhe: 3.197 ± 0.333
4.858LysGly: 4.858 ± 0.383
1.586LysHis: 1.586 ± 0.249
5.131LysIle: 5.131 ± 0.398
6.494LysLys: 6.494 ± 0.521
6.94LysLeu: 6.94 ± 0.442
1.958LysMet: 1.958 ± 0.198
6.072LysAsn: 6.072 ± 0.452
2.379LysPro: 2.379 ± 0.279
2.999LysGln: 2.999 ± 0.272
2.974LysArg: 2.974 ± 0.281
5.825LysSer: 5.825 ± 0.506
4.337LysThr: 4.337 ± 0.295
5.676LysVal: 5.676 ± 0.405
0.967LysTrp: 0.967 ± 0.132
5.081LysTyr: 5.081 ± 0.305
0.0LysXaa: 0.0 ± 0.0
Leu
4.759LeuAla: 4.759 ± 0.346
0.52LeuCys: 0.52 ± 0.121
6.543LeuAsp: 6.543 ± 0.451
5.676LeuGlu: 5.676 ± 0.472
3.272LeuPhe: 3.272 ± 0.285
4.784LeuGly: 4.784 ± 0.41
0.793LeuHis: 0.793 ± 0.138
5.329LeuIle: 5.329 ± 0.38
7.113LeuLys: 7.113 ± 0.527
6.89LeuLeu: 6.89 ± 0.489
1.363LeuMet: 1.363 ± 0.218
6.196LeuAsn: 6.196 ± 0.486
3.049LeuPro: 3.049 ± 0.277
2.355LeuGln: 2.355 ± 0.206
2.355LeuArg: 2.355 ± 0.255
7.535LeuSer: 7.535 ± 0.518
4.957LeuThr: 4.957 ± 0.352
4.957LeuVal: 4.957 ± 0.338
0.62LeuTrp: 0.62 ± 0.116
3.842LeuTyr: 3.842 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
1.561MetAla: 1.561 ± 0.222
0.124MetCys: 0.124 ± 0.069
1.661MetAsp: 1.661 ± 0.193
1.462MetGlu: 1.462 ± 0.202
0.644MetPhe: 0.644 ± 0.105
1.239MetGly: 1.239 ± 0.175
0.149MetHis: 0.149 ± 0.057
1.512MetIle: 1.512 ± 0.208
2.082MetLys: 2.082 ± 0.277
2.082MetLeu: 2.082 ± 0.239
0.446MetMet: 0.446 ± 0.132
1.958MetAsn: 1.958 ± 0.218
0.694MetPro: 0.694 ± 0.145
0.917MetGln: 0.917 ± 0.141
0.62MetArg: 0.62 ± 0.163
1.636MetSer: 1.636 ± 0.227
1.19MetThr: 1.19 ± 0.163
1.239MetVal: 1.239 ± 0.162
0.223MetTrp: 0.223 ± 0.079
0.892MetTyr: 0.892 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
3.594AsnAla: 3.594 ± 0.327
0.57AsnCys: 0.57 ± 0.138
4.015AsnAsp: 4.015 ± 0.312
3.842AsnGlu: 3.842 ± 0.301
2.875AsnPhe: 2.875 ± 0.245
4.09AsnGly: 4.09 ± 0.377
1.487AsnHis: 1.487 ± 0.191
5.998AsnIle: 5.998 ± 0.429
8.031AsnLys: 8.031 ± 0.501
5.626AsnLeu: 5.626 ± 0.409
2.355AsnMet: 2.355 ± 0.266
6.692AsnAsn: 6.692 ± 0.659
2.801AsnPro: 2.801 ± 0.291
2.602AsnGln: 2.602 ± 0.284
2.677AsnArg: 2.677 ± 0.317
6.866AsnSer: 6.866 ± 0.591
5.007AsnThr: 5.007 ± 0.403
3.743AsnVal: 3.743 ± 0.293
0.595AsnTrp: 0.595 ± 0.123
4.263AsnTyr: 4.263 ± 0.33
0.0AsnXaa: 0.0 ± 0.0
Pro
1.512ProAla: 1.512 ± 0.202
0.074ProCys: 0.074 ± 0.045
2.528ProAsp: 2.528 ± 0.257
1.809ProGlu: 1.809 ± 0.261
1.239ProPhe: 1.239 ± 0.185
1.363ProGly: 1.363 ± 0.186
0.372ProHis: 0.372 ± 0.094
2.082ProIle: 2.082 ± 0.227
2.132ProLys: 2.132 ± 0.251
2.602ProLeu: 2.602 ± 0.24
0.421ProMet: 0.421 ± 0.121
2.132ProAsn: 2.132 ± 0.273
0.397ProPro: 0.397 ± 0.094
1.636ProGln: 1.636 ± 0.353
0.694ProArg: 0.694 ± 0.158
1.933ProSer: 1.933 ± 0.24
2.057ProThr: 2.057 ± 0.282
2.28ProVal: 2.28 ± 0.274
0.297ProTrp: 0.297 ± 0.087
1.661ProTyr: 1.661 ± 0.208
0.0ProXaa: 0.0 ± 0.0
Gln
2.429GlnAla: 2.429 ± 0.27
0.149GlnCys: 0.149 ± 0.058
2.305GlnAsp: 2.305 ± 0.27
2.206GlnGlu: 2.206 ± 0.254
0.967GlnPhe: 0.967 ± 0.159
1.884GlnGly: 1.884 ± 0.243
0.57GlnHis: 0.57 ± 0.126
1.71GlnIle: 1.71 ± 0.239
2.206GlnLys: 2.206 ± 0.298
3.123GlnLeu: 3.123 ± 0.324
0.57GlnMet: 0.57 ± 0.101
1.809GlnAsn: 1.809 ± 0.241
1.041GlnPro: 1.041 ± 0.212
1.338GlnGln: 1.338 ± 0.295
0.991GlnArg: 0.991 ± 0.179
2.429GlnSer: 2.429 ± 0.28
1.438GlnThr: 1.438 ± 0.257
2.231GlnVal: 2.231 ± 0.221
0.446GlnTrp: 0.446 ± 0.109
2.379GlnTyr: 2.379 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
1.289ArgAla: 1.289 ± 0.209
0.173ArgCys: 0.173 ± 0.073
2.008ArgAsp: 2.008 ± 0.266
2.032ArgGlu: 2.032 ± 0.267
1.586ArgPhe: 1.586 ± 0.192
1.438ArgGly: 1.438 ± 0.182
0.223ArgHis: 0.223 ± 0.076
2.454ArgIle: 2.454 ± 0.25
2.702ArgLys: 2.702 ± 0.273
3.098ArgLeu: 3.098 ± 0.288
0.719ArgMet: 0.719 ± 0.15
1.859ArgAsn: 1.859 ± 0.213
0.62ArgPro: 0.62 ± 0.117
1.413ArgGln: 1.413 ± 0.178
1.115ArgArg: 1.115 ± 0.241
1.661ArgSer: 1.661 ± 0.224
1.537ArgThr: 1.537 ± 0.224
2.404ArgVal: 2.404 ± 0.259
0.372ArgTrp: 0.372 ± 0.083
1.958ArgTyr: 1.958 ± 0.211
0.0ArgXaa: 0.0 ± 0.0
Ser
3.668SerAla: 3.668 ± 0.478
0.397SerCys: 0.397 ± 0.107
6.048SerAsp: 6.048 ± 0.386
4.684SerGlu: 4.684 ± 0.399
3.321SerPhe: 3.321 ± 0.244
5.8SerGly: 5.8 ± 0.533
0.942SerHis: 0.942 ± 0.176
6.94SerIle: 6.94 ± 0.624
6.593SerLys: 6.593 ± 0.424
6.89SerLeu: 6.89 ± 0.438
1.611SerMet: 1.611 ± 0.215
6.618SerAsn: 6.618 ± 0.463
2.156SerPro: 2.156 ± 0.297
2.751SerGln: 2.751 ± 0.278
2.454SerArg: 2.454 ± 0.275
7.262SerSer: 7.262 ± 0.597
4.263SerThr: 4.263 ± 0.349
4.164SerVal: 4.164 ± 0.299
1.016SerTrp: 1.016 ± 0.167
4.61SerTyr: 4.61 ± 0.404
0.0SerXaa: 0.0 ± 0.0
Thr
3.123ThrAla: 3.123 ± 0.428
0.57ThrCys: 0.57 ± 0.125
4.164ThrAsp: 4.164 ± 0.345
2.875ThrGlu: 2.875 ± 0.266
2.776ThrPhe: 2.776 ± 0.225
3.544ThrGly: 3.544 ± 0.293
0.818ThrHis: 0.818 ± 0.134
4.932ThrIle: 4.932 ± 0.344
4.114ThrLys: 4.114 ± 0.333
4.66ThrLeu: 4.66 ± 0.303
0.644ThrMet: 0.644 ± 0.132
4.313ThrAsn: 4.313 ± 0.345
2.355ThrPro: 2.355 ± 0.27
2.008ThrGln: 2.008 ± 0.284
1.661ThrArg: 1.661 ± 0.226
5.106ThrSer: 5.106 ± 0.517
4.238ThrThr: 4.238 ± 0.541
3.792ThrVal: 3.792 ± 0.341
0.867ThrTrp: 0.867 ± 0.171
2.826ThrTyr: 2.826 ± 0.27
0.0ThrXaa: 0.0 ± 0.0
Val
3.197ValAla: 3.197 ± 0.268
0.471ValCys: 0.471 ± 0.092
4.214ValAsp: 4.214 ± 0.332
2.875ValGlu: 2.875 ± 0.28
2.85ValPhe: 2.85 ± 0.248
3.346ValGly: 3.346 ± 0.289
0.644ValHis: 0.644 ± 0.13
4.189ValIle: 4.189 ± 0.273
4.164ValLys: 4.164 ± 0.364
4.139ValLeu: 4.139 ± 0.269
1.413ValMet: 1.413 ± 0.163
4.387ValAsn: 4.387 ± 0.388
1.809ValPro: 1.809 ± 0.215
1.735ValGln: 1.735 ± 0.238
1.438ValArg: 1.438 ± 0.202
5.849ValSer: 5.849 ± 0.486
4.511ValThr: 4.511 ± 0.384
2.826ValVal: 2.826 ± 0.268
0.768ValTrp: 0.768 ± 0.15
3.222ValTyr: 3.222 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
0.471TrpAla: 0.471 ± 0.118
0.074TrpCys: 0.074 ± 0.038
0.917TrpAsp: 0.917 ± 0.155
0.57TrpGlu: 0.57 ± 0.126
0.57TrpPhe: 0.57 ± 0.109
0.768TrpGly: 0.768 ± 0.147
0.149TrpHis: 0.149 ± 0.061
0.793TrpIle: 0.793 ± 0.147
0.496TrpLys: 0.496 ± 0.096
1.19TrpLeu: 1.19 ± 0.236
0.322TrpMet: 0.322 ± 0.091
0.892TrpAsn: 0.892 ± 0.161
0.149TrpPro: 0.149 ± 0.05
0.595TrpGln: 0.595 ± 0.122
0.198TrpArg: 0.198 ± 0.072
0.397TrpSer: 0.397 ± 0.116
0.421TrpThr: 0.421 ± 0.103
0.793TrpVal: 0.793 ± 0.151
0.223TrpTrp: 0.223 ± 0.081
0.694TrpTyr: 0.694 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.479TyrAla: 2.479 ± 0.279
0.397TyrCys: 0.397 ± 0.09
3.445TyrAsp: 3.445 ± 0.288
2.33TyrGlu: 2.33 ± 0.289
2.206TyrPhe: 2.206 ± 0.233
2.578TyrGly: 2.578 ± 0.251
0.991TyrHis: 0.991 ± 0.171
4.635TyrIle: 4.635 ± 0.422
4.337TyrLys: 4.337 ± 0.383
4.982TyrLeu: 4.982 ± 0.41
1.388TyrMet: 1.388 ± 0.18
4.263TyrAsn: 4.263 ± 0.33
1.933TyrPro: 1.933 ± 0.225
2.032TyrGln: 2.032 ± 0.308
2.206TyrArg: 2.206 ± 0.259
4.808TyrSer: 4.808 ± 0.359
3.42TyrThr: 3.42 ± 0.332
3.247TyrVal: 3.247 ± 0.309
0.545TyrTrp: 0.545 ± 0.118
2.949TyrTyr: 2.949 ± 0.27
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 188 proteins (40347 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski