Amino acid dipepetide frequency for Virgibacillus massiliensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.562AlaAla: 5.562 ± 0.083	0.636AlaCys: 0.636 ± 0.024	3.471AlaAsp: 3.471 ± 0.061	4.227AlaGlu: 4.227 ± 0.06	3.494AlaPhe: 3.494 ± 0.058	5.078AlaGly: 5.078 ± 0.075	1.219AlaHis: 1.219 ± 0.029	6.351AlaIle: 6.351 ± 0.078	4.521AlaLys: 4.521 ± 0.063	6.805AlaLeu: 6.805 ± 0.097	2.09AlaMet: 2.09 ± 0.042	3.253AlaAsn: 3.253 ± 0.052	2.0AlaPro: 2.0 ± 0.038	2.058AlaGln: 2.058 ± 0.043	2.335AlaArg: 2.335 ± 0.055	4.274AlaSer: 4.274 ± 0.06	3.87AlaThr: 3.87 ± 0.06	5.058AlaVal: 5.058 ± 0.079	0.679AlaTrp: 0.679 ± 0.023	2.61AlaTyr: 2.61 ± 0.057	0.0AlaXaa: 0.0 ± 0.0
Cys	0.387CysAla: 0.387 ± 0.02	0.095CysCys: 0.095 ± 0.009	0.373CysAsp: 0.373 ± 0.02	0.389CysGlu: 0.389 ± 0.021	0.336CysPhe: 0.336 ± 0.018	0.578CysGly: 0.578 ± 0.026	0.192CysHis: 0.192 ± 0.014	0.575CysIle: 0.575 ± 0.022	0.376CysLys: 0.376 ± 0.02	0.606CysLeu: 0.606 ± 0.024	0.194CysMet: 0.194 ± 0.013	0.334CysAsn: 0.334 ± 0.016	0.291CysPro: 0.291 ± 0.015	0.228CysGln: 0.228 ± 0.014	0.248CysArg: 0.248 ± 0.014	0.452CysSer: 0.452 ± 0.02	0.381CysThr: 0.381 ± 0.018	0.376CysVal: 0.376 ± 0.018	0.071CysTrp: 0.071 ± 0.007	0.26CysTyr: 0.26 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.406AspAla: 3.406 ± 0.055	0.328AspCys: 0.328 ± 0.017	2.634AspAsp: 2.634 ± 0.056	4.127AspGlu: 4.127 ± 0.062	2.38AspPhe: 2.38 ± 0.046	3.379AspGly: 3.379 ± 0.06	1.293AspHis: 1.293 ± 0.033	4.446AspIle: 4.446 ± 0.069	3.507AspLys: 3.507 ± 0.059	4.852AspLeu: 4.852 ± 0.077	1.499AspMet: 1.499 ± 0.03	2.349AspAsn: 2.349 ± 0.056	2.108AspPro: 2.108 ± 0.041	2.372AspGln: 2.372 ± 0.045	2.25AspArg: 2.25 ± 0.045	2.58AspSer: 2.58 ± 0.047	2.641AspThr: 2.641 ± 0.046	3.71AspVal: 3.71 ± 0.058	0.691AspTrp: 0.691 ± 0.026	2.337AspTyr: 2.337 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	5.408GluAla: 5.408 ± 0.066	0.343GluCys: 0.343 ± 0.019	3.82GluAsp: 3.82 ± 0.067	6.488GluGlu: 6.488 ± 0.094	2.373GluPhe: 2.373 ± 0.049	4.208GluGly: 4.208 ± 0.066	1.431GluHis: 1.431 ± 0.037	5.444GluIle: 5.444 ± 0.07	5.854GluLys: 5.854 ± 0.083	6.672GluLeu: 6.672 ± 0.084	2.228GluMet: 2.228 ± 0.043	3.82GluAsn: 3.82 ± 0.061	1.936GluPro: 1.936 ± 0.041	3.637GluGln: 3.637 ± 0.065	3.26GluArg: 3.26 ± 0.061	3.558GluSer: 3.558 ± 0.061	3.861GluThr: 3.861 ± 0.058	4.834GluVal: 4.834 ± 0.066	0.789GluTrp: 0.789 ± 0.026	2.11GluTyr: 2.11 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.047PheAla: 3.047 ± 0.058	0.302PheCys: 0.302 ± 0.015	2.334PheAsp: 2.334 ± 0.046	2.621PheGlu: 2.621 ± 0.05	2.261PhePhe: 2.261 ± 0.05	3.162PheGly: 3.162 ± 0.055	1.097PheHis: 1.097 ± 0.034	4.318PheIle: 4.318 ± 0.088	2.17PheLys: 2.17 ± 0.042	4.447PheLeu: 4.447 ± 0.086	1.209PheMet: 1.209 ± 0.033	1.905PheAsn: 1.905 ± 0.04	1.644PhePro: 1.644 ± 0.039	1.788PheGln: 1.788 ± 0.038	1.511PheArg: 1.511 ± 0.036	3.164PheSer: 3.164 ± 0.052	2.699PheThr: 2.699 ± 0.052	3.055PheVal: 3.055 ± 0.055	0.505PheTrp: 0.505 ± 0.022	1.724PheTyr: 1.724 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.691GlyAla: 4.691 ± 0.077	0.555GlyCys: 0.555 ± 0.023	3.257GlyAsp: 3.257 ± 0.053	4.442GlyGlu: 4.442 ± 0.063	3.377GlyPhe: 3.377 ± 0.059	4.643GlyGly: 4.643 ± 0.084	1.316GlyHis: 1.316 ± 0.034	6.256GlyIle: 6.256 ± 0.085	4.686GlyLys: 4.686 ± 0.069	6.438GlyLeu: 6.438 ± 0.084	2.147GlyMet: 2.147 ± 0.041	2.992GlyAsn: 2.992 ± 0.06	1.746GlyPro: 1.746 ± 0.046	1.993GlyGln: 1.993 ± 0.039	2.341GlyArg: 2.341 ± 0.049	4.073GlySer: 4.073 ± 0.058	3.913GlyThr: 3.913 ± 0.066	4.909GlyVal: 4.909 ± 0.065	0.836GlyTrp: 0.836 ± 0.031	2.914GlyTyr: 2.914 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	1.545HisAla: 1.545 ± 0.036	0.19HisCys: 0.19 ± 0.013	1.089HisAsp: 1.089 ± 0.03	1.343HisGlu: 1.343 ± 0.033	1.088HisPhe: 1.088 ± 0.028	1.482HisGly: 1.482 ± 0.033	0.728HisHis: 0.728 ± 0.023	1.689HisIle: 1.689 ± 0.039	1.005HisLys: 1.005 ± 0.026	2.226HisLeu: 2.226 ± 0.044	0.613HisMet: 0.613 ± 0.022	0.805HisAsn: 0.805 ± 0.028	1.168HisPro: 1.168 ± 0.028	1.046HisGln: 1.046 ± 0.029	0.941HisArg: 0.941 ± 0.031	1.235HisSer: 1.235 ± 0.031	1.173HisThr: 1.173 ± 0.029	1.488HisVal: 1.488 ± 0.032	0.234HisTrp: 0.234 ± 0.012	0.911HisTyr: 0.911 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.5IleAla: 6.5 ± 0.095	0.647IleCys: 0.647 ± 0.025	4.722IleAsp: 4.722 ± 0.065	5.588IleGlu: 5.588 ± 0.072	3.404IlePhe: 3.404 ± 0.068	6.453IleGly: 6.453 ± 0.089	1.936IleHis: 1.936 ± 0.044	6.846IleIle: 6.846 ± 0.107	4.551IleLys: 4.551 ± 0.067	7.225IleLeu: 7.225 ± 0.109	1.928IleMet: 1.928 ± 0.042	3.818IleAsn: 3.818 ± 0.062	3.585IlePro: 3.585 ± 0.055	3.287IleGln: 3.287 ± 0.059	3.132IleArg: 3.132 ± 0.047	5.342IleSer: 5.342 ± 0.064	4.973IleThr: 4.973 ± 0.061	5.465IleVal: 5.465 ± 0.072	0.756IleTrp: 0.756 ± 0.024	2.728IleTyr: 2.728 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	4.352LysAla: 4.352 ± 0.06	0.297LysCys: 0.297 ± 0.015	3.968LysAsp: 3.968 ± 0.062	6.322LysGlu: 6.322 ± 0.094	1.6LysPhe: 1.6 ± 0.036	4.033LysGly: 4.033 ± 0.063	1.55LysHis: 1.55 ± 0.035	4.083LysIle: 4.083 ± 0.063	5.198LysLys: 5.198 ± 0.072	5.558LysLeu: 5.558 ± 0.07	1.981LysMet: 1.981 ± 0.037	3.22LysAsn: 3.22 ± 0.05	2.131LysPro: 2.131 ± 0.042	3.976LysGln: 3.976 ± 0.059	3.214LysArg: 3.214 ± 0.053	3.307LysSer: 3.307 ± 0.053	3.368LysThr: 3.368 ± 0.049	4.081LysVal: 4.081 ± 0.054	0.729LysTrp: 0.729 ± 0.026	2.086LysTyr: 2.086 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.934LeuAla: 6.934 ± 0.089	0.601LeuCys: 0.601 ± 0.022	4.787LeuAsp: 4.787 ± 0.067	6.236LeuGlu: 6.236 ± 0.073	4.941LeuPhe: 4.941 ± 0.085	6.3LeuGly: 6.3 ± 0.092	2.001LeuHis: 2.001 ± 0.039	7.577LeuIle: 7.577 ± 0.097	5.695LeuLys: 5.695 ± 0.072	9.487LeuLeu: 9.487 ± 0.137	2.47LeuMet: 2.47 ± 0.042	4.143LeuAsn: 4.143 ± 0.069	3.899LeuPro: 3.899 ± 0.058	3.861LeuGln: 3.861 ± 0.058	3.404LeuArg: 3.404 ± 0.058	6.573LeuSer: 6.573 ± 0.077	5.6LeuThr: 5.6 ± 0.071	5.707LeuVal: 5.707 ± 0.073	0.825LeuTrp: 0.825 ± 0.026	3.119LeuTyr: 3.119 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	1.997MetAla: 1.997 ± 0.038	0.118MetCys: 0.118 ± 0.01	1.631MetAsp: 1.631 ± 0.041	2.222MetGlu: 2.222 ± 0.042	1.121MetPhe: 1.121 ± 0.034	1.786MetGly: 1.786 ± 0.038	0.553MetHis: 0.553 ± 0.022	2.25MetIle: 2.25 ± 0.048	2.392MetLys: 2.392 ± 0.046	2.562MetLeu: 2.562 ± 0.055	0.862MetMet: 0.862 ± 0.027	1.66MetAsn: 1.66 ± 0.038	1.007MetPro: 1.007 ± 0.023	1.101MetGln: 1.101 ± 0.031	1.089MetArg: 1.089 ± 0.034	1.616MetSer: 1.616 ± 0.032	1.625MetThr: 1.625 ± 0.033	1.831MetVal: 1.831 ± 0.043	0.201MetTrp: 0.201 ± 0.015	0.862MetTyr: 0.862 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.865AsnAla: 2.865 ± 0.052	0.329AsnCys: 0.329 ± 0.02	2.512AsnAsp: 2.512 ± 0.05	3.72AsnGlu: 3.72 ± 0.057	1.734AsnPhe: 1.734 ± 0.037	3.411AsnGly: 3.411 ± 0.051	1.302AsnHis: 1.302 ± 0.034	3.462AsnIle: 3.462 ± 0.062	3.323AsnLys: 3.323 ± 0.053	3.848AsnLeu: 3.848 ± 0.066	1.31AsnMet: 1.31 ± 0.034	2.517AsnAsn: 2.517 ± 0.05	2.141AsnPro: 2.141 ± 0.046	2.592AsnGln: 2.592 ± 0.048	2.102AsnArg: 2.102 ± 0.044	2.362AsnSer: 2.362 ± 0.051	2.523AsnThr: 2.523 ± 0.049	3.035AsnVal: 3.035 ± 0.054	0.641AsnTrp: 0.641 ± 0.022	2.029AsnTyr: 2.029 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	2.248ProAla: 2.248 ± 0.046	0.201ProCys: 0.201 ± 0.013	2.078ProAsp: 2.078 ± 0.05	2.885ProGlu: 2.885 ± 0.049	1.936ProPhe: 1.936 ± 0.039	2.279ProGly: 2.279 ± 0.044	0.784ProHis: 0.784 ± 0.024	3.187ProIle: 3.187 ± 0.048	2.033ProLys: 2.033 ± 0.039	3.246ProLeu: 3.246 ± 0.052	0.929ProMet: 0.929 ± 0.024	1.839ProAsn: 1.839 ± 0.041	0.974ProPro: 0.974 ± 0.031	1.083ProGln: 1.083 ± 0.031	1.016ProArg: 1.016 ± 0.028	2.366ProSer: 2.366 ± 0.05	2.14ProThr: 2.14 ± 0.048	2.666ProVal: 2.666 ± 0.046	0.385ProTrp: 0.385 ± 0.016	1.501ProTyr: 1.501 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.238GlnAla: 3.238 ± 0.05	0.191GlnCys: 0.191 ± 0.011	2.009GlnAsp: 2.009 ± 0.037	3.203GlnGlu: 3.203 ± 0.053	1.689GlnPhe: 1.689 ± 0.037	2.267GlnGly: 2.267 ± 0.044	0.936GlnHis: 0.936 ± 0.03	2.883GlnIle: 2.883 ± 0.047	2.596GlnLys: 2.596 ± 0.049	4.623GlnLeu: 4.623 ± 0.069	1.239GlnMet: 1.239 ± 0.032	1.642GlnAsn: 1.642 ± 0.04	1.381GlnPro: 1.381 ± 0.039	2.4GlnGln: 2.4 ± 0.058	1.66GlnArg: 1.66 ± 0.039	2.382GlnSer: 2.382 ± 0.043	2.41GlnThr: 2.41 ± 0.045	2.659GlnVal: 2.659 ± 0.049	0.463GlnTrp: 0.463 ± 0.02	1.287GlnTyr: 1.287 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.171ArgAla: 2.171 ± 0.041	0.251ArgCys: 0.251 ± 0.015	1.897ArgAsp: 1.897 ± 0.038	2.886ArgGlu: 2.886 ± 0.057	1.855ArgPhe: 1.855 ± 0.039	2.159ArgGly: 2.159 ± 0.037	0.745ArgHis: 0.745 ± 0.025	3.237ArgIle: 3.237 ± 0.048	3.047ArgLys: 3.047 ± 0.051	3.765ArgLeu: 3.765 ± 0.065	1.323ArgMet: 1.323 ± 0.039	2.05ArgAsn: 2.05 ± 0.038	1.166ArgPro: 1.166 ± 0.032	1.533ArgGln: 1.533 ± 0.036	1.605ArgArg: 1.605 ± 0.043	2.252ArgSer: 2.252 ± 0.039	2.087ArgThr: 2.087 ± 0.053	2.37ArgVal: 2.37 ± 0.046	0.421ArgTrp: 0.421 ± 0.017	1.498ArgTyr: 1.498 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	3.534SerAla: 3.534 ± 0.052	0.379SerCys: 0.379 ± 0.018	3.038SerAsp: 3.038 ± 0.054	3.953SerGlu: 3.953 ± 0.058	3.268SerPhe: 3.268 ± 0.061	4.348SerGly: 4.348 ± 0.064	1.237SerHis: 1.237 ± 0.032	5.529SerIle: 5.529 ± 0.067	3.752SerLys: 3.752 ± 0.059	5.795SerLeu: 5.795 ± 0.068	1.822SerMet: 1.822 ± 0.036	2.99SerAsn: 2.99 ± 0.049	2.056SerPro: 2.056 ± 0.044	2.016SerGln: 2.016 ± 0.039	2.155SerArg: 2.155 ± 0.042	3.96SerSer: 3.96 ± 0.066	3.261SerThr: 3.261 ± 0.055	3.87SerVal: 3.87 ± 0.062	0.667SerTrp: 0.667 ± 0.021	2.374SerTyr: 2.374 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.058ThrAla: 4.058 ± 0.056	0.363ThrCys: 0.363 ± 0.017	2.982ThrAsp: 2.982 ± 0.049	3.696ThrGlu: 3.696 ± 0.058	2.669ThrPhe: 2.669 ± 0.055	4.244ThrGly: 4.244 ± 0.092	1.078ThrHis: 1.078 ± 0.028	5.175ThrIle: 5.175 ± 0.06	3.583ThrLys: 3.583 ± 0.052	4.975ThrLeu: 4.975 ± 0.064	1.488ThrMet: 1.488 ± 0.036	2.94ThrAsn: 2.94 ± 0.049	2.24ThrPro: 2.24 ± 0.041	1.49ThrGln: 1.49 ± 0.04	1.745ThrArg: 1.745 ± 0.036	3.583ThrSer: 3.583 ± 0.057	3.23ThrThr: 3.23 ± 0.055	4.022ThrVal: 4.022 ± 0.062	0.609ThrTrp: 0.609 ± 0.022	2.2ThrTyr: 2.2 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	4.73ValAla: 4.73 ± 0.067	0.539ValCys: 0.539 ± 0.02	3.681ValAsp: 3.681 ± 0.065	4.544ValGlu: 4.544 ± 0.069	3.049ValPhe: 3.049 ± 0.054	4.43ValGly: 4.43 ± 0.071	1.34ValHis: 1.34 ± 0.029	5.94ValIle: 5.94 ± 0.08	4.004ValLys: 4.004 ± 0.066	6.309ValLeu: 6.309 ± 0.085	1.839ValMet: 1.839 ± 0.042	3.242ValAsn: 3.242 ± 0.058	2.534ValPro: 2.534 ± 0.045	2.325ValGln: 2.325 ± 0.043	2.336ValArg: 2.336 ± 0.044	4.25ValSer: 4.25 ± 0.066	4.077ValThr: 4.077 ± 0.059	4.642ValVal: 4.642 ± 0.061	0.628ValTrp: 0.628 ± 0.025	2.254ValTyr: 2.254 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.62TrpAla: 0.62 ± 0.023	0.074TrpCys: 0.074 ± 0.007	0.586TrpAsp: 0.586 ± 0.023	0.744TrpGlu: 0.744 ± 0.023	0.533TrpPhe: 0.533 ± 0.021	0.709TrpGly: 0.709 ± 0.022	0.215TrpHis: 0.215 ± 0.013	0.935TrpIle: 0.935 ± 0.027	0.764TrpLys: 0.764 ± 0.022	1.138TrpLeu: 1.138 ± 0.03	0.368TrpMet: 0.368 ± 0.015	0.61TrpAsn: 0.61 ± 0.024	0.262TrpPro: 0.262 ± 0.016	0.408TrpGln: 0.408 ± 0.02	0.376TrpArg: 0.376 ± 0.017	0.602TrpSer: 0.602 ± 0.022	0.538TrpThr: 0.538 ± 0.023	0.674TrpVal: 0.674 ± 0.025	0.165TrpTrp: 0.165 ± 0.013	0.406TrpTyr: 0.406 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.297TyrAla: 2.297 ± 0.044	0.303TyrCys: 0.303 ± 0.017	2.019TyrAsp: 2.019 ± 0.045	2.438TyrGlu: 2.438 ± 0.042	1.855TyrPhe: 1.855 ± 0.04	2.578TyrGly: 2.578 ± 0.048	1.046TyrHis: 1.046 ± 0.033	2.703TyrIle: 2.703 ± 0.052	1.996TyrLys: 1.996 ± 0.042	3.582TyrLeu: 3.582 ± 0.055	0.945TyrMet: 0.945 ± 0.031	1.63TyrAsn: 1.63 ± 0.038	1.508TyrPro: 1.508 ± 0.038	1.965TyrGln: 1.965 ± 0.044	1.602TyrArg: 1.602 ± 0.037	2.074TyrSer: 2.074 ± 0.039	1.999TyrThr: 1.999 ± 0.04	2.241TyrVal: 2.241 ± 0.041	0.432TyrTrp: 0.432 ± 0.019	1.612TyrTyr: 1.612 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4389 proteins (1246616 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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