Dryobates pubescens (Downy woodpecker) (Picoides pubescens)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13097 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093GNW6|A0A093GNW6_DRYPU Leucine-rich repeat-containing protein 58 (Fragment) OS=Dryobates pubescens OX=118200 GN=N307_11395 PE=4 SV=1
DDD2 pKa = 5.37DDD4 pKa = 3.8VAFCVLAADDD14 pKa = 4.74EE15 pKa = 4.51EE16 pKa = 4.83DD17 pKa = 3.81EE18 pKa = 5.55DDD20 pKa = 3.34ALQIHHH26 pKa = 6.13TLIQAFCCEEE36 pKa = 3.79AIDDD40 pKa = 3.79VRR42 pKa = 11.84VSDDD46 pKa = 3.5GKKK49 pKa = 9.83AAIVGPSEEE58 pKa = 4.11EE59 pKa = 4.26GEEE62 pKa = 4.11RR63 pKa = 11.84DDD65 pKa = 3.68HHH67 pKa = 6.98ILITV

Molecular weight: 7.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093IZ63|A0A093IZ63_DRYPU Uncharacterized protein (Fragment) OS=Dryobates pubescens OX=118200 GN=N307_13949 PE=4 SV=1
RR1 pKa = 7.28ARR3 pKa = 11.84QGRR6 pKa = 11.84ARR8 pKa = 11.84QGRR11 pKa = 11.84ARR13 pKa = 11.84QGRR16 pKa = 11.84ARR18 pKa = 11.84QGRR21 pKa = 11.84ARR23 pKa = 11.84QGRR26 pKa = 11.84ARR28 pKa = 11.84QGRR31 pKa = 11.84ARR33 pKa = 11.84QGRR36 pKa = 11.84ARR38 pKa = 11.84QGRR41 pKa = 11.84ARR43 pKa = 11.84QGRR46 pKa = 11.84ARR48 pKa = 11.84QGRR51 pKa = 11.84EE52 pKa = 3.53RR53 pKa = 11.84QGG55 pKa = 3.04

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13097

0

13097

5781682

33

6237

441.5

49.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.669 ± 0.021

2.316 ± 0.019

4.943 ± 0.014

7.201 ± 0.026

3.853 ± 0.017

6.055 ± 0.026

2.571 ± 0.011

4.794 ± 0.018

6.255 ± 0.025

9.859 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.009

3.952 ± 0.015

5.385 ± 0.027

4.709 ± 0.018

5.327 ± 0.017

8.18 ± 0.03

5.274 ± 0.016

6.28 ± 0.018

1.229 ± 0.009

2.942 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski