Amino acid dipepetide frequency for Dryobates pubescens (Downy woodpecker) (Picoides pubescens)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.391AlaAla: 6.391 ± 0.052	1.437AlaCys: 1.437 ± 0.021	3.057AlaAsp: 3.057 ± 0.024	4.864AlaGlu: 4.864 ± 0.033	2.651AlaPhe: 2.651 ± 0.023	4.253AlaGly: 4.253 ± 0.037	1.45AlaHis: 1.45 ± 0.018	3.027AlaIle: 3.027 ± 0.027	3.723AlaLys: 3.723 ± 0.03	6.795AlaLeu: 6.795 ± 0.042	1.514AlaMet: 1.514 ± 0.016	2.236AlaAsn: 2.236 ± 0.02	3.14AlaPro: 3.14 ± 0.034	2.909AlaGln: 2.909 ± 0.028	3.135AlaArg: 3.135 ± 0.033	5.287AlaSer: 5.287 ± 0.035	3.348AlaThr: 3.348 ± 0.029	5.078AlaVal: 5.078 ± 0.043	0.751AlaTrp: 0.751 ± 0.013	1.644AlaTyr: 1.644 ± 0.018	0.001AlaXaa: 0.001 ± 0.0
Cys	1.299CysAla: 1.299 ± 0.02	0.707CysCys: 0.707 ± 0.017	1.069CysAsp: 1.069 ± 0.023	1.335CysGlu: 1.335 ± 0.025	0.924CysPhe: 0.924 ± 0.015	1.533CysGly: 1.533 ± 0.026	0.668CysHis: 0.668 ± 0.012	1.153CysIle: 1.153 ± 0.02	1.299CysLys: 1.299 ± 0.02	2.305CysLeu: 2.305 ± 0.026	0.455CysMet: 0.455 ± 0.012	0.906CysAsn: 0.906 ± 0.016	1.327CysPro: 1.327 ± 0.026	1.134CysGln: 1.134 ± 0.021	1.297CysArg: 1.297 ± 0.02	2.107CysSer: 2.107 ± 0.03	1.236CysThr: 1.236 ± 0.021	1.406CysVal: 1.406 ± 0.024	0.315CysTrp: 0.315 ± 0.007	0.689CysTyr: 0.689 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	2.875AspAla: 2.875 ± 0.022	1.149AspCys: 1.149 ± 0.02	2.739AspAsp: 2.739 ± 0.035	3.583AspGlu: 3.583 ± 0.03	2.226AspPhe: 2.226 ± 0.022	3.275AspGly: 3.275 ± 0.033	1.155AspHis: 1.155 ± 0.016	2.87AspIle: 2.87 ± 0.027	2.689AspLys: 2.689 ± 0.025	5.015AspLeu: 5.015 ± 0.035	1.124AspMet: 1.124 ± 0.014	1.864AspAsn: 1.864 ± 0.021	2.658AspPro: 2.658 ± 0.025	1.808AspGln: 1.808 ± 0.019	2.35AspArg: 2.35 ± 0.027	4.072AspSer: 4.072 ± 0.032	2.465AspThr: 2.465 ± 0.024	3.221AspVal: 3.221 ± 0.023	0.645AspTrp: 0.645 ± 0.012	1.651AspTyr: 1.651 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	4.881GluAla: 4.881 ± 0.036	1.341GluCys: 1.341 ± 0.029	4.431GluAsp: 4.431 ± 0.034	8.031GluGlu: 8.031 ± 0.08	2.183GluPhe: 2.183 ± 0.021	4.104GluGly: 4.104 ± 0.039	1.571GluHis: 1.571 ± 0.018	3.377GluIle: 3.377 ± 0.026	5.658GluLys: 5.658 ± 0.057	6.437GluLeu: 6.437 ± 0.05	1.757GluMet: 1.757 ± 0.018	3.264GluAsn: 3.264 ± 0.029	2.634GluPro: 2.634 ± 0.028	3.267GluGln: 3.267 ± 0.032	4.046GluArg: 4.046 ± 0.047	4.441GluSer: 4.441 ± 0.034	3.427GluThr: 3.427 ± 0.027	4.45GluVal: 4.45 ± 0.034	0.917GluTrp: 0.917 ± 0.022	1.798GluTyr: 1.798 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.21PheAla: 2.21 ± 0.024	1.008PheCys: 1.008 ± 0.014	1.822PheAsp: 1.822 ± 0.02	2.108PheGlu: 2.108 ± 0.022	1.788PhePhe: 1.788 ± 0.021	2.329PheGly: 2.329 ± 0.023	1.106PheHis: 1.106 ± 0.013	2.05PheIle: 2.05 ± 0.023	1.999PheLys: 1.999 ± 0.021	4.204PheLeu: 4.204 ± 0.037	0.796PheMet: 0.796 ± 0.012	1.478PheAsn: 1.478 ± 0.019	1.982PhePro: 1.982 ± 0.023	1.83PheGln: 1.83 ± 0.019	1.928PheArg: 1.928 ± 0.02	3.434PheSer: 3.434 ± 0.024	2.242PheThr: 2.242 ± 0.022	2.358PheVal: 2.358 ± 0.022	0.524PheTrp: 0.524 ± 0.01	1.33PheTyr: 1.33 ± 0.015	0.001PheXaa: 0.001 ± 0.0
Gly	3.821GlyAla: 3.821 ± 0.036	1.305GlyCys: 1.305 ± 0.018	2.982GlyAsp: 2.982 ± 0.025	3.913GlyGlu: 3.913 ± 0.042	2.476GlyPhe: 2.476 ± 0.028	4.086GlyGly: 4.086 ± 0.047	1.579GlyHis: 1.579 ± 0.019	3.054GlyIle: 3.054 ± 0.028	4.05GlyLys: 4.05 ± 0.035	5.384GlyLeu: 5.384 ± 0.041	1.505GlyMet: 1.505 ± 0.023	2.514GlyAsn: 2.514 ± 0.021	2.822GlyPro: 2.822 ± 0.058	2.582GlyGln: 2.582 ± 0.026	3.443GlyArg: 3.443 ± 0.045	5.277GlySer: 5.277 ± 0.045	3.5GlyThr: 3.5 ± 0.034	3.555GlyVal: 3.555 ± 0.029	0.812GlyTrp: 0.812 ± 0.016	1.888GlyTyr: 1.888 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	1.337HisAla: 1.337 ± 0.017	0.767HisCys: 0.767 ± 0.014	0.912HisAsp: 0.912 ± 0.012	1.334HisGlu: 1.334 ± 0.016	1.098HisPhe: 1.098 ± 0.014	1.532HisGly: 1.532 ± 0.019	0.919HisHis: 0.919 ± 0.018	1.359HisIle: 1.359 ± 0.017	1.381HisLys: 1.381 ± 0.018	2.873HisLeu: 2.873 ± 0.026	0.57HisMet: 0.57 ± 0.01	0.959HisAsn: 0.959 ± 0.014	1.561HisPro: 1.561 ± 0.022	1.21HisGln: 1.21 ± 0.015	1.499HisArg: 1.499 ± 0.02	2.258HisSer: 2.258 ± 0.02	1.388HisThr: 1.388 ± 0.025	1.504HisVal: 1.504 ± 0.019	0.377HisTrp: 0.377 ± 0.008	0.871HisTyr: 0.871 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	2.969IleAla: 2.969 ± 0.025	1.208IleCys: 1.208 ± 0.02	2.263IleAsp: 2.263 ± 0.021	2.808IleGlu: 2.808 ± 0.026	2.084IlePhe: 2.084 ± 0.021	2.387IleGly: 2.387 ± 0.025	1.397IleHis: 1.397 ± 0.018	2.599IleIle: 2.599 ± 0.028	2.916IleLys: 2.916 ± 0.026	4.887IleLeu: 4.887 ± 0.04	1.038IleMet: 1.038 ± 0.013	2.049IleAsn: 2.049 ± 0.022	2.791IlePro: 2.791 ± 0.029	2.415IleGln: 2.415 ± 0.024	2.459IleArg: 2.459 ± 0.021	3.905IleSer: 3.905 ± 0.027	2.757IleThr: 2.757 ± 0.027	2.847IleVal: 2.847 ± 0.026	0.574IleTrp: 0.574 ± 0.011	1.585IleTyr: 1.585 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	4.231LysAla: 4.231 ± 0.033	1.217LysCys: 1.217 ± 0.018	3.362LysAsp: 3.362 ± 0.028	5.664LysGlu: 5.664 ± 0.053	1.896LysPhe: 1.896 ± 0.02	3.497LysGly: 3.497 ± 0.042	1.571LysHis: 1.571 ± 0.02	3.03LysIle: 3.03 ± 0.029	5.168LysLys: 5.168 ± 0.055	5.637LysLeu: 5.637 ± 0.044	1.5LysMet: 1.5 ± 0.018	2.572LysAsn: 2.572 ± 0.026	3.091LysPro: 3.091 ± 0.03	2.93LysGln: 2.93 ± 0.029	3.595LysArg: 3.595 ± 0.036	4.126LysSer: 4.126 ± 0.036	3.218LysThr: 3.218 ± 0.025	3.744LysVal: 3.744 ± 0.029	0.672LysTrp: 0.672 ± 0.012	1.824LysTyr: 1.824 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	6.557LeuAla: 6.557 ± 0.047	2.326LeuCys: 2.326 ± 0.025	4.818LeuAsp: 4.818 ± 0.036	7.114LeuGlu: 7.114 ± 0.049	3.619LeuPhe: 3.619 ± 0.034	5.44LeuGly: 5.44 ± 0.038	2.747LeuHis: 2.747 ± 0.027	4.167LeuIle: 4.167 ± 0.031	6.307LeuLys: 6.307 ± 0.046	10.502LeuLeu: 10.502 ± 0.068	2.019LeuMet: 2.019 ± 0.023	3.758LeuAsn: 3.758 ± 0.028	5.648LeuPro: 5.648 ± 0.042	5.823LeuGln: 5.823 ± 0.046	5.309LeuArg: 5.309 ± 0.036	7.925LeuSer: 7.925 ± 0.052	4.979LeuThr: 4.979 ± 0.037	5.588LeuVal: 5.588 ± 0.036	1.126LeuTrp: 1.126 ± 0.015	2.816LeuTyr: 2.816 ± 0.024	0.001LeuXaa: 0.001 ± 0.001
Met	1.664MetAla: 1.664 ± 0.018	0.447MetCys: 0.447 ± 0.01	1.27MetAsp: 1.27 ± 0.016	1.993MetGlu: 1.993 ± 0.023	0.81MetPhe: 0.81 ± 0.012	1.247MetGly: 1.247 ± 0.016	0.525MetHis: 0.525 ± 0.012	0.929MetIle: 0.929 ± 0.013	1.569MetLys: 1.569 ± 0.019	2.033MetLeu: 2.033 ± 0.023	0.575MetMet: 0.575 ± 0.011	0.946MetAsn: 0.946 ± 0.016	1.033MetPro: 1.033 ± 0.014	0.995MetGln: 0.995 ± 0.015	1.048MetArg: 1.048 ± 0.014	1.52MetSer: 1.52 ± 0.019	1.104MetThr: 1.104 ± 0.015	1.419MetVal: 1.419 ± 0.018	0.246MetTrp: 0.246 ± 0.006	0.646MetTyr: 0.646 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.294AsnAla: 2.294 ± 0.022	0.95AsnCys: 0.95 ± 0.016	1.631AsnAsp: 1.631 ± 0.02	2.435AsnGlu: 2.435 ± 0.023	1.56AsnPhe: 1.56 ± 0.018	2.863AsnGly: 2.863 ± 0.029	0.964AsnHis: 0.964 ± 0.015	2.342AsnIle: 2.342 ± 0.021	2.44AsnLys: 2.44 ± 0.022	3.978AsnLeu: 3.978 ± 0.029	0.958AsnMet: 0.958 ± 0.013	1.694AsnAsn: 1.694 ± 0.019	2.228AsnPro: 2.228 ± 0.021	1.671AsnGln: 1.671 ± 0.019	1.952AsnArg: 1.952 ± 0.019	3.285AsnSer: 3.285 ± 0.029	2.147AsnThr: 2.147 ± 0.025	2.39AsnVal: 2.39 ± 0.02	0.48AsnTrp: 0.48 ± 0.01	1.259AsnTyr: 1.259 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	4.039ProAla: 4.039 ± 0.038	1.148ProCys: 1.148 ± 0.023	2.55ProAsp: 2.55 ± 0.024	3.945ProGlu: 3.945 ± 0.032	1.943ProPhe: 1.943 ± 0.021	3.961ProGly: 3.961 ± 0.081	1.291ProHis: 1.291 ± 0.022	1.906ProIle: 1.906 ± 0.021	2.741ProLys: 2.741 ± 0.028	4.794ProLeu: 4.794 ± 0.038	0.927ProMet: 0.927 ± 0.014	1.849ProAsn: 1.849 ± 0.021	4.393ProPro: 4.393 ± 0.072	2.386ProGln: 2.386 ± 0.026	2.614ProArg: 2.614 ± 0.025	5.002ProSer: 5.002 ± 0.048	2.575ProThr: 2.575 ± 0.029	3.683ProVal: 3.683 ± 0.032	0.616ProTrp: 0.616 ± 0.013	1.481ProTyr: 1.481 ± 0.021	0.001ProXaa: 0.001 ± 0.0
Gln	3.238GlnAla: 3.238 ± 0.028	1.01GlnCys: 1.01 ± 0.019	2.239GlnAsp: 2.239 ± 0.022	3.747GlnGlu: 3.747 ± 0.035	1.475GlnPhe: 1.475 ± 0.017	2.653GlnGly: 2.653 ± 0.034	1.365GlnHis: 1.365 ± 0.018	2.151GlnIle: 2.151 ± 0.021	3.093GlnLys: 3.093 ± 0.028	4.748GlnLeu: 4.748 ± 0.035	1.092GlnMet: 1.092 ± 0.015	1.939GlnAsn: 1.939 ± 0.021	2.432GlnPro: 2.432 ± 0.03	3.047GlnGln: 3.047 ± 0.042	2.742GlnArg: 2.742 ± 0.027	3.175GlnSer: 3.175 ± 0.028	2.299GlnThr: 2.299 ± 0.023	2.813GlnVal: 2.813 ± 0.023	0.545GlnTrp: 0.545 ± 0.012	1.285GlnTyr: 1.285 ± 0.016	0.001GlnXaa: 0.001 ± 0.0
Arg	3.322ArgAla: 3.322 ± 0.028	1.175ArgCys: 1.175 ± 0.022	2.624ArgAsp: 2.624 ± 0.027	3.809ArgGlu: 3.809 ± 0.038	1.953ArgPhe: 1.953 ± 0.021	3.155ArgGly: 3.155 ± 0.033	1.437ArgHis: 1.437 ± 0.019	2.518ArgIle: 2.518 ± 0.022	3.967ArgLys: 3.967 ± 0.042	5.028ArgLeu: 5.028 ± 0.036	1.184ArgMet: 1.184 ± 0.017	2.176ArgAsn: 2.176 ± 0.019	2.441ArgPro: 2.441 ± 0.026	2.557ArgGln: 2.557 ± 0.028	4.052ArgArg: 4.052 ± 0.046	3.997ArgSer: 3.997 ± 0.041	2.647ArgThr: 2.647 ± 0.025	2.996ArgVal: 2.996 ± 0.028	0.651ArgTrp: 0.651 ± 0.01	1.578ArgTyr: 1.578 ± 0.016	0.0ArgXaa: 0.0 ± 0.0
Ser	5.201SerAla: 5.201 ± 0.034	1.967SerCys: 1.967 ± 0.028	3.887SerAsp: 3.887 ± 0.039	5.07SerGlu: 5.07 ± 0.038	3.144SerPhe: 3.144 ± 0.029	4.964SerGly: 4.964 ± 0.037	2.055SerHis: 2.055 ± 0.022	3.497SerIle: 3.497 ± 0.031	4.422SerLys: 4.422 ± 0.036	8.12SerLeu: 8.12 ± 0.048	1.574SerMet: 1.574 ± 0.019	2.9SerAsn: 2.9 ± 0.025	5.224SerPro: 5.224 ± 0.053	3.651SerGln: 3.651 ± 0.032	4.126SerArg: 4.126 ± 0.043	9.282SerSer: 9.282 ± 0.085	4.46SerThr: 4.46 ± 0.034	4.992SerVal: 4.992 ± 0.034	1.014SerTrp: 1.014 ± 0.016	2.248SerTyr: 2.248 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	3.881ThrAla: 3.881 ± 0.03	1.351ThrCys: 1.351 ± 0.026	2.621ThrAsp: 2.621 ± 0.027	3.631ThrGlu: 3.631 ± 0.028	2.17ThrPhe: 2.17 ± 0.02	3.402ThrGly: 3.402 ± 0.031	1.185ThrHis: 1.185 ± 0.02	2.514ThrIle: 2.514 ± 0.022	2.822ThrLys: 2.822 ± 0.023	5.169ThrLeu: 5.169 ± 0.036	1.101ThrMet: 1.101 ± 0.015	1.878ThrAsn: 1.878 ± 0.018	3.061ThrPro: 3.061 ± 0.033	2.131ThrGln: 2.131 ± 0.021	2.287ThrArg: 2.287 ± 0.02	4.458ThrSer: 4.458 ± 0.033	2.902ThrThr: 2.902 ± 0.034	3.979ThrVal: 3.979 ± 0.034	0.665ThrTrp: 0.665 ± 0.012	1.531ThrTyr: 1.531 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	4.193ValAla: 4.193 ± 0.029	1.613ValCys: 1.613 ± 0.026	3.035ValAsp: 3.035 ± 0.029	4.031ValGlu: 4.031 ± 0.035	2.654ValPhe: 2.654 ± 0.024	3.415ValGly: 3.415 ± 0.029	1.584ValHis: 1.584 ± 0.018	3.172ValIle: 3.172 ± 0.026	3.754ValLys: 3.754 ± 0.033	6.484ValLeu: 6.484 ± 0.042	1.355ValMet: 1.355 ± 0.017	2.491ValAsn: 2.491 ± 0.022	3.549ValPro: 3.549 ± 0.032	2.859ValGln: 2.859 ± 0.026	3.008ValArg: 3.008 ± 0.028	4.912ValSer: 4.912 ± 0.033	3.769ValThr: 3.769 ± 0.033	4.346ValVal: 4.346 ± 0.032	0.751ValTrp: 0.751 ± 0.013	1.826ValTyr: 1.826 ± 0.022	0.001ValXaa: 0.001 ± 0.0
Trp	0.723TrpAla: 0.723 ± 0.011	0.263TrpCys: 0.263 ± 0.007	0.678TrpAsp: 0.678 ± 0.015	0.795TrpGlu: 0.795 ± 0.013	0.479TrpPhe: 0.479 ± 0.011	0.674TrpGly: 0.674 ± 0.013	0.335TrpHis: 0.335 ± 0.01	0.608TrpIle: 0.608 ± 0.012	0.875TrpLys: 0.875 ± 0.014	1.223TrpLeu: 1.223 ± 0.016	0.318TrpMet: 0.318 ± 0.006	0.791TrpAsn: 0.791 ± 0.021	0.455TrpPro: 0.455 ± 0.009	0.56TrpGln: 0.56 ± 0.008	0.674TrpArg: 0.674 ± 0.011	0.916TrpSer: 0.916 ± 0.017	0.652TrpThr: 0.652 ± 0.013	0.7TrpVal: 0.7 ± 0.013	0.199TrpTrp: 0.199 ± 0.007	0.375TrpTyr: 0.375 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.563TyrAla: 1.563 ± 0.017	0.775TyrCys: 0.775 ± 0.013	1.444TyrAsp: 1.444 ± 0.018	1.803TyrGlu: 1.803 ± 0.02	1.394TyrPhe: 1.394 ± 0.018	1.775TyrGly: 1.775 ± 0.021	0.804TyrHis: 0.804 ± 0.011	1.614TyrIle: 1.614 ± 0.017	1.671TyrLys: 1.671 ± 0.021	2.976TyrLeu: 2.976 ± 0.025	0.659TyrMet: 0.659 ± 0.011	1.254TyrAsn: 1.254 ± 0.015	1.375TyrPro: 1.375 ± 0.015	1.325TyrGln: 1.325 ± 0.016	1.702TyrArg: 1.702 ± 0.016	2.42TyrSer: 2.42 ± 0.026	1.624TyrThr: 1.624 ± 0.02	1.733TyrVal: 1.733 ± 0.018	0.414TyrTrp: 0.414 ± 0.013	1.099TyrTyr: 1.099 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.001XaaTrp: 0.001 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.034XaaXaa: 0.034 ± 0.005

Statistics based on 13097 proteins (5781682 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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