Halotalea alkalilenta

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halotalea

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3715 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172YIW1|A0A172YIW1_9GAMM Uncharacterized protein OS=Halotalea alkalilenta OX=376489 GN=A5892_18230 PE=4 SV=1
MM1 pKa = 7.29QLTKK5 pKa = 10.37TLGTSLIGALALGALSGQALAQDD28 pKa = 3.68KK29 pKa = 10.89GSVNIAYY36 pKa = 9.74VEE38 pKa = 3.97WADD41 pKa = 4.59NIASSNVLRR50 pKa = 11.84VVLEE54 pKa = 3.85QQGYY58 pKa = 7.01QVEE61 pKa = 4.7TTSLSAAAMWQAVAFGDD78 pKa = 4.19ADD80 pKa = 4.38MMTAWLPTTHH90 pKa = 6.34AQYY93 pKa = 11.14YY94 pKa = 9.96SQLQDD99 pKa = 3.16QVEE102 pKa = 4.15NLGPNFEE109 pKa = 4.24GTRR112 pKa = 11.84LGLVVPSYY120 pKa = 11.01VEE122 pKa = 3.95EE123 pKa = 4.26VNSIEE128 pKa = 5.03DD129 pKa = 3.82LASHH133 pKa = 7.07ADD135 pKa = 3.63EE136 pKa = 5.31FDD138 pKa = 3.79DD139 pKa = 6.23AITGIDD145 pKa = 3.57PGAGIMSITEE155 pKa = 3.81QAMEE159 pKa = 5.15DD160 pKa = 3.73YY161 pKa = 11.24GLDD164 pKa = 3.81DD165 pKa = 5.66LEE167 pKa = 4.69LTTGSDD173 pKa = 3.48ATMTAALGSAIANQQPIVVTGWTPHH198 pKa = 4.46WMFSRR203 pKa = 11.84WDD205 pKa = 3.64LKK207 pKa = 11.18YY208 pKa = 11.17LEE210 pKa = 5.4DD211 pKa = 3.98PQNAFGEE218 pKa = 4.44AEE220 pKa = 4.16QIDD223 pKa = 4.24TVVRR227 pKa = 11.84EE228 pKa = 4.17GLEE231 pKa = 4.29DD232 pKa = 5.15DD233 pKa = 5.58DD234 pKa = 5.51PEE236 pKa = 5.24AYY238 pKa = 10.52CIFNNFRR245 pKa = 11.84WSPDD249 pKa = 2.78EE250 pKa = 4.0MGAMMLLNEE259 pKa = 4.35QQGSDD264 pKa = 3.3PYY266 pKa = 11.11EE267 pKa = 4.07NAKK270 pKa = 9.68QWVADD275 pKa = 3.74HH276 pKa = 6.11QALVDD281 pKa = 3.93SWTEE285 pKa = 4.02GCQQGG290 pKa = 3.74

Molecular weight: 31.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172YBM3|A0A172YBM3_9GAMM Uncharacterized protein OS=Halotalea alkalilenta OX=376489 GN=A5892_02705 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.31RR26 pKa = 11.84GRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3715

0

3715

1214334

44

2862

326.9

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.698 ± 0.05

0.943 ± 0.011

5.558 ± 0.031

6.358 ± 0.038

3.576 ± 0.024

8.356 ± 0.038

2.238 ± 0.021

4.872 ± 0.029

2.308 ± 0.031

11.69 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.017

2.312 ± 0.021

4.993 ± 0.027

3.648 ± 0.026

7.951 ± 0.043

5.728 ± 0.027

4.568 ± 0.026

7.146 ± 0.033

1.419 ± 0.017

2.355 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski