Planctomyces sp. SCGC AG-212-M04

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctomyces; unclassified Planctomyces

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177QYL0|A0A177QYL0_9PLAN Uncharacterized protein OS=Planctomyces sp. SCGC AG-212-M04 OX=1799657 GN=AYO47_01165 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84LFAQQPRR10 pKa = 11.84MRR12 pKa = 11.84RR13 pKa = 11.84TRR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.74FRR19 pKa = 11.84GCFTVQALEE28 pKa = 4.2ARR30 pKa = 11.84ILLDD34 pKa = 3.72GEE36 pKa = 4.26EE37 pKa = 4.85DD38 pKa = 3.74PPYY41 pKa = 10.67DD42 pKa = 4.57PPPAAADD49 pKa = 3.87DD50 pKa = 4.0YY51 pKa = 11.61FFAGDD56 pKa = 3.72YY57 pKa = 10.37NQLSDD62 pKa = 4.15YY63 pKa = 10.77QDD65 pKa = 3.22TVAGNDD71 pKa = 3.58DD72 pKa = 4.22YY73 pKa = 11.88EE74 pKa = 4.87VGDD77 pKa = 3.99RR78 pKa = 11.84FSVLSDD84 pKa = 3.54PEE86 pKa = 4.37YY87 pKa = 10.6GTLDD91 pKa = 3.59MQSDD95 pKa = 4.2GSFTYY100 pKa = 10.26TPDD103 pKa = 3.67GEE105 pKa = 4.6HH106 pKa = 7.11PGLFSFTYY114 pKa = 10.55QIDD117 pKa = 3.86DD118 pKa = 3.92GVNTPSEE125 pKa = 3.89ATAWLEE131 pKa = 4.06LYY133 pKa = 10.46NPPPEE138 pKa = 4.51ANEE141 pKa = 3.88DD142 pKa = 3.23VGYY145 pKa = 10.71FSLDD149 pKa = 3.25QGTMTIDD156 pKa = 3.4AAHH159 pKa = 6.81GLLVNDD165 pKa = 4.48SLDD168 pKa = 3.57SRR170 pKa = 11.84EE171 pKa = 4.41SNTASLWEE179 pKa = 3.92QPDD182 pKa = 3.63YY183 pKa = 11.65GSASVSSDD191 pKa = 2.48GSFTYY196 pKa = 10.39TPDD199 pKa = 3.4PEE201 pKa = 4.4YY202 pKa = 10.72LGEE205 pKa = 3.94VSFIYY210 pKa = 10.42KK211 pKa = 10.63LSDD214 pKa = 3.3GKK216 pKa = 11.07GGEE219 pKa = 4.01AFAEE223 pKa = 4.44VKK225 pKa = 10.46IEE227 pKa = 3.96VGKK230 pKa = 8.88VTVEE234 pKa = 3.69RR235 pKa = 11.84CTEE238 pKa = 3.83YY239 pKa = 10.85QGDD242 pKa = 3.64VWIGEE247 pKa = 4.53YY248 pKa = 10.18IPLQAVVTGGTGGNVGAMTYY268 pKa = 9.54SWSLSGQTLKK278 pKa = 11.06DD279 pKa = 3.48YY280 pKa = 11.6VNDD283 pKa = 3.55YY284 pKa = 8.17FTASDD289 pKa = 4.04GSSQPRR295 pKa = 11.84AYY297 pKa = 9.74PLPLSAADD305 pKa = 4.06LASEE309 pKa = 4.59YY310 pKa = 9.53ITFGWTSDD318 pKa = 3.38GAQTATVTVSLNGQTSTGQIEE339 pKa = 4.57INVQKK344 pKa = 9.33PTFDD348 pKa = 3.81YY349 pKa = 10.06YY350 pKa = 11.85ADD352 pKa = 3.39MGGINLNNLTQQQGGRR368 pKa = 11.84SGASLGTINPYY379 pKa = 9.36QAGIVFWAPFDD390 pKa = 3.43TWNPDD395 pKa = 2.98PRR397 pKa = 11.84QVGSMGLIQTGAFSASRR414 pKa = 11.84TLADD418 pKa = 3.44GTVQEE423 pKa = 5.05ISASGADD430 pKa = 3.29NGGDD434 pKa = 3.41HH435 pKa = 7.61DD436 pKa = 5.07AAGNDD441 pKa = 3.69APSLKK446 pKa = 10.44LMPNARR452 pKa = 11.84SYY454 pKa = 10.57SVEE457 pKa = 4.0YY458 pKa = 11.18SFDD461 pKa = 3.58TYY463 pKa = 11.24LKK465 pKa = 9.83WKK467 pKa = 9.81GPRR470 pKa = 11.84PGFWVGVSVVHH481 pKa = 5.73WWFSYY486 pKa = 10.43EE487 pKa = 3.87ATSADD492 pKa = 4.07GITWTLTSTSGTLNQRR508 pKa = 11.84IDD510 pKa = 3.98GVDD513 pKa = 4.1GIYY516 pKa = 10.49FPTYY520 pKa = 10.5AGDD523 pKa = 3.76IAWAAGGQTNDD534 pKa = 3.8HH535 pKa = 7.23DD536 pKa = 4.59LGGWGTPDD544 pKa = 2.92

Molecular weight: 58.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177R6E5|A0A177R6E5_9PLAN Uncharacterized protein OS=Planctomyces sp. SCGC AG-212-M04 OX=1799657 GN=AYO47_00310 PE=4 SV=1
MM1 pKa = 7.71GGLLEE6 pKa = 4.12KK7 pKa = 10.58AFRR10 pKa = 11.84SSTRR14 pKa = 11.84LARR17 pKa = 11.84TWSAAGLDD25 pKa = 3.95LFYY28 pKa = 10.76PPACASCRR36 pKa = 11.84CDD38 pKa = 2.99LAASFEE44 pKa = 4.64SRR46 pKa = 11.84FCNDD50 pKa = 2.6CRR52 pKa = 11.84NLFVPVVADD61 pKa = 3.97RR62 pKa = 11.84CRR64 pKa = 11.84RR65 pKa = 11.84CSAPVGPHH73 pKa = 6.86LDD75 pKa = 3.42TSAGCVHH82 pKa = 7.42CSRR85 pKa = 11.84EE86 pKa = 3.43RR87 pKa = 11.84WKK89 pKa = 10.61FDD91 pKa = 3.06RR92 pKa = 11.84VFSLGPYY99 pKa = 10.24ADD101 pKa = 4.33ALRR104 pKa = 11.84DD105 pKa = 3.06ACLRR109 pKa = 11.84MKK111 pKa = 10.69RR112 pKa = 11.84AGTEE116 pKa = 3.68PLAAALGEE124 pKa = 4.05EE125 pKa = 5.61LFRR128 pKa = 11.84QWHH131 pKa = 5.54EE132 pKa = 3.91VLLSAKK138 pKa = 9.65YY139 pKa = 10.53DD140 pKa = 4.26LIVPVPHH147 pKa = 7.05HH148 pKa = 5.3WRR150 pKa = 11.84QRR152 pKa = 11.84VWRR155 pKa = 11.84TQLVPVTLGRR165 pKa = 11.84VLSQAIGVPLSLHH178 pKa = 6.28LVRR181 pKa = 11.84KK182 pKa = 9.95AKK184 pKa = 8.65FTKK187 pKa = 9.77QQSTLSVTEE196 pKa = 3.61RR197 pKa = 11.84RR198 pKa = 11.84ANLRR202 pKa = 11.84GAFRR206 pKa = 11.84RR207 pKa = 11.84VPGRR211 pKa = 11.84RR212 pKa = 11.84IAGGRR217 pKa = 11.84VLVVDD222 pKa = 4.99DD223 pKa = 4.53VLTTGTTSDD232 pKa = 2.81RR233 pKa = 11.84VARR236 pKa = 11.84VLRR239 pKa = 11.84EE240 pKa = 3.94MGASSIDD247 pKa = 3.52VAVVARR253 pKa = 11.84GLGHH257 pKa = 6.21SS258 pKa = 3.91

Molecular weight: 28.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1771

0

1771

596544

58

2932

336.8

36.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.479 ± 0.064

1.106 ± 0.021

5.715 ± 0.039

6.153 ± 0.052

3.728 ± 0.035

7.977 ± 0.055

2.102 ± 0.026

4.717 ± 0.045

4.218 ± 0.052

9.718 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.156 ± 0.027

2.905 ± 0.035

5.551 ± 0.049

3.681 ± 0.052

6.999 ± 0.057

6.105 ± 0.047

5.467 ± 0.043

7.369 ± 0.05

1.585 ± 0.031

2.269 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski