Planomicrobium sp. YIM 101495

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Planomicrobium; unclassified Planomicrobium

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2829 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L6H2W7|A0A6L6H2W7_9BACL Na+/H+ antiporter NhaC family protein OS=Planomicrobium sp. YIM 101495 OX=2665160 GN=GIW82_12200 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.63KK3 pKa = 9.61MIGIAAVIVAIFALIIVLTNMSNKK27 pKa = 9.6EE28 pKa = 3.76KK29 pKa = 10.51LANNPYY35 pKa = 9.28DD36 pKa = 4.11TDD38 pKa = 4.17DD39 pKa = 5.3LDD41 pKa = 4.44PATIDD46 pKa = 3.66QLDD49 pKa = 3.81DD50 pKa = 3.65EE51 pKa = 5.01NYY53 pKa = 10.1QNIVLPEE60 pKa = 3.94EE61 pKa = 4.17LNEE64 pKa = 4.06QIEE67 pKa = 4.61SGEE70 pKa = 3.88ATTVYY75 pKa = 10.04FFSPTCQYY83 pKa = 10.37CQQTTPVLMPVADD96 pKa = 4.73DD97 pKa = 3.68MDD99 pKa = 4.61VDD101 pKa = 3.99VLQYY105 pKa = 11.66NLLEE109 pKa = 4.33YY110 pKa = 10.74DD111 pKa = 4.16QGWQQYY117 pKa = 9.62FIEE120 pKa = 4.54ATPTLIHH127 pKa = 6.44FEE129 pKa = 4.05NGEE132 pKa = 4.16EE133 pKa = 4.02VSRR136 pKa = 11.84WVGAQPKK143 pKa = 9.9EE144 pKa = 4.08NIEE147 pKa = 3.99EE148 pKa = 4.16FFNEE152 pKa = 4.23VVLKK156 pKa = 10.85

Molecular weight: 17.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L6H1X2|A0A6L6H1X2_9BACL S8 family serine peptidase OS=Planomicrobium sp. YIM 101495 OX=2665160 GN=GIW82_06960 PE=3 SV=1
MM1 pKa = 7.67SLRR4 pKa = 11.84TYY6 pKa = 10.41QPNKK10 pKa = 8.74RR11 pKa = 11.84KK12 pKa = 9.54HH13 pKa = 5.99SKK15 pKa = 8.91VHH17 pKa = 5.68GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 9.66NGRR29 pKa = 11.84RR30 pKa = 11.84VLAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.81GRR40 pKa = 11.84KK41 pKa = 8.75VLSAA45 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2829

0

2829

847762

20

2079

299.7

33.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.546 ± 0.05

0.563 ± 0.01

5.343 ± 0.041

8.088 ± 0.06

4.541 ± 0.04

7.386 ± 0.04

2.04 ± 0.023

6.968 ± 0.042

5.637 ± 0.042

9.797 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.916 ± 0.022

3.542 ± 0.032

3.799 ± 0.025

3.616 ± 0.029

4.593 ± 0.041

5.686 ± 0.033

5.477 ± 0.039

7.284 ± 0.04

1.011 ± 0.017

3.164 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski