Escherichia phage RB69 (Bacteriophage RB69)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Mosigvirus; Escherichia virus RB69

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 273 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7Y554|Q7Y554_BPR69 Uncharacterized protein RB69ORF089c OS=Escherichia phage RB69 OX=12353 GN=RB69ORF089c PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.47SSINSGVKK10 pKa = 8.78PWYY13 pKa = 7.7SARR16 pKa = 11.84WEE18 pKa = 4.27TVEE21 pKa = 4.2PDD23 pKa = 3.13VEE25 pKa = 4.36TVYY28 pKa = 11.21NDD30 pKa = 3.62EE31 pKa = 4.34EE32 pKa = 4.31PCYY35 pKa = 10.29DD36 pKa = 3.53EE37 pKa = 5.38PSINEE42 pKa = 4.48LIDD45 pKa = 3.24MEE47 pKa = 4.41MGRR50 pKa = 11.84DD51 pKa = 3.96YY52 pKa = 11.25YY53 pKa = 11.57ASS55 pKa = 3.4

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7Y558|Q7Y558_BPR69 NrdD anaerobic NTP reductase large subunit OS=Escherichia phage RB69 OX=12353 GN=nrdD PE=4 SV=1
MM1 pKa = 6.94KK2 pKa = 10.19TEE4 pKa = 4.39LFYY7 pKa = 11.54GEE9 pKa = 4.61RR10 pKa = 11.84KK11 pKa = 9.88NGQKK15 pKa = 9.24SWKK18 pKa = 9.62FVMTGAPNNPTMTTSIISVTRR39 pKa = 11.84PTKK42 pKa = 10.79KK43 pKa = 9.68MIRR46 pKa = 11.84QYY48 pKa = 11.54KK49 pKa = 8.78RR50 pKa = 11.84FHH52 pKa = 6.58RR53 pKa = 11.84DD54 pKa = 2.98FYY56 pKa = 11.72NVYY59 pKa = 10.65

Molecular weight: 7.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

273

0

273

52284

29

1277

191.5

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.729 ± 0.155

1.069 ± 0.064

6.304 ± 0.105

7.26 ± 0.161

4.418 ± 0.089

6.149 ± 0.208

1.702 ± 0.081

7.182 ± 0.127

7.436 ± 0.222

7.519 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.086

5.564 ± 0.117

3.529 ± 0.111

3.259 ± 0.089

4.372 ± 0.097

6.413 ± 0.12

6.086 ± 0.208

6.706 ± 0.129

1.398 ± 0.055

4.261 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski