Amino acid dipepetide frequency for Escherichia phage RB69 (Bacteriophage RB69)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.973AlaAla: 4.973 ± 0.393
0.402AlaCys: 0.402 ± 0.074
3.864AlaAsp: 3.864 ± 0.274
5.413AlaGlu: 5.413 ± 0.34
2.372AlaPhe: 2.372 ± 0.2
4.323AlaGly: 4.323 ± 0.375
1.014AlaHis: 1.014 ± 0.136
4.667AlaIle: 4.667 ± 0.316
5.03AlaLys: 5.03 ± 0.354
6.121AlaLeu: 6.121 ± 0.34
1.358AlaMet: 1.358 ± 0.161
3.347AlaAsn: 3.347 ± 0.219
2.563AlaPro: 2.563 ± 0.227
2.448AlaGln: 2.448 ± 0.235
2.926AlaArg: 2.926 ± 0.243
4.667AlaSer: 4.667 ± 0.278
3.978AlaThr: 3.978 ± 0.443
4.17AlaVal: 4.17 ± 0.283
0.975AlaTrp: 0.975 ± 0.123
2.678AlaTyr: 2.678 ± 0.238
0.0AlaXaa: 0.0 ± 0.0
Cys
0.631CysAla: 0.631 ± 0.125
0.172CysCys: 0.172 ± 0.059
0.593CysAsp: 0.593 ± 0.103
0.746CysGlu: 0.746 ± 0.123
0.421CysPhe: 0.421 ± 0.099
0.727CysGly: 0.727 ± 0.117
0.268CysHis: 0.268 ± 0.065
0.746CysIle: 0.746 ± 0.121
0.727CysLys: 0.727 ± 0.118
0.631CysLeu: 0.631 ± 0.117
0.191CysMet: 0.191 ± 0.066
0.574CysAsn: 0.574 ± 0.106
0.593CysPro: 0.593 ± 0.106
0.344CysGln: 0.344 ± 0.087
0.727CysArg: 0.727 ± 0.126
0.822CysSer: 0.822 ± 0.124
0.536CysThr: 0.536 ± 0.096
0.612CysVal: 0.612 ± 0.118
0.115CysTrp: 0.115 ± 0.05
0.516CysTyr: 0.516 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
4.457AspAla: 4.457 ± 0.259
0.612AspCys: 0.612 ± 0.108
3.959AspAsp: 3.959 ± 0.291
4.514AspGlu: 4.514 ± 0.342
3.519AspPhe: 3.519 ± 0.274
4.877AspGly: 4.877 ± 0.295
0.765AspHis: 0.765 ± 0.132
5.03AspIle: 5.03 ± 0.258
3.883AspLys: 3.883 ± 0.308
4.724AspLeu: 4.724 ± 0.32
1.626AspMet: 1.626 ± 0.195
2.946AspAsn: 2.946 ± 0.251
2.295AspPro: 2.295 ± 0.242
1.645AspGln: 1.645 ± 0.181
2.41AspArg: 2.41 ± 0.219
3.959AspSer: 3.959 ± 0.218
3.079AspThr: 3.079 ± 0.299
4.495AspVal: 4.495 ± 0.264
1.109AspTrp: 1.109 ± 0.176
3.137AspTyr: 3.137 ± 0.272
0.0AspXaa: 0.0 ± 0.0
Glu
5.126GluAla: 5.126 ± 0.302
1.109GluCys: 1.109 ± 0.159
4.284GluAsp: 4.284 ± 0.272
5.03GluGlu: 5.03 ± 0.356
3.634GluPhe: 3.634 ± 0.343
3.864GluGly: 3.864 ± 0.296
1.09GluHis: 1.09 ± 0.149
6.388GluIle: 6.388 ± 0.345
4.801GluLys: 4.801 ± 0.337
6.388GluLeu: 6.388 ± 0.412
2.161GluMet: 2.161 ± 0.213
3.691GluAsn: 3.691 ± 0.267
1.97GluPro: 1.97 ± 0.229
2.429GluGln: 2.429 ± 0.254
2.946GluArg: 2.946 ± 0.265
4.15GluSer: 4.15 ± 0.25
4.361GluThr: 4.361 ± 0.329
4.839GluVal: 4.839 ± 0.254
0.861GluTrp: 0.861 ± 0.125
3.481GluTyr: 3.481 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
2.888PheAla: 2.888 ± 0.226
0.44PheCys: 0.44 ± 0.103
3.137PheAsp: 3.137 ± 0.252
3.806PheGlu: 3.806 ± 0.288
1.454PhePhe: 1.454 ± 0.17
2.525PheGly: 2.525 ± 0.214
0.708PheHis: 0.708 ± 0.122
3.099PheIle: 3.099 ± 0.256
3.902PheLys: 3.902 ± 0.341
2.563PheLeu: 2.563 ± 0.19
1.205PheMet: 1.205 ± 0.153
2.946PheAsn: 2.946 ± 0.229
1.033PhePro: 1.033 ± 0.143
1.339PheGln: 1.339 ± 0.171
1.932PheArg: 1.932 ± 0.187
3.06PheSer: 3.06 ± 0.223
2.792PheThr: 2.792 ± 0.229
2.85PheVal: 2.85 ± 0.233
0.593PheTrp: 0.593 ± 0.12
1.913PheTyr: 1.913 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
3.194GlyAla: 3.194 ± 0.301
0.478GlyCys: 0.478 ± 0.104
4.38GlyAsp: 4.38 ± 0.347
3.749GlyGlu: 3.749 ± 0.258
2.506GlyPhe: 2.506 ± 0.23
4.17GlyGly: 4.17 ± 0.58
1.186GlyHis: 1.186 ± 0.163
4.361GlyIle: 4.361 ± 0.251
4.074GlyLys: 4.074 ± 0.286
5.355GlyLeu: 5.355 ± 0.369
1.607GlyMet: 1.607 ± 0.194
3.328GlyAsn: 3.328 ± 0.42
1.76GlyPro: 1.76 ± 0.179
2.123GlyGln: 2.123 ± 0.208
2.716GlyArg: 2.716 ± 0.237
3.844GlySer: 3.844 ± 0.318
4.437GlyThr: 4.437 ± 0.373
4.17GlyVal: 4.17 ± 0.338
0.918GlyTrp: 0.918 ± 0.135
3.137GlyTyr: 3.137 ± 0.267
0.0GlyXaa: 0.0 ± 0.0
His
0.975HisAla: 0.975 ± 0.146
0.363HisCys: 0.363 ± 0.092
1.014HisAsp: 1.014 ± 0.154
1.014HisGlu: 1.014 ± 0.134
0.765HisPhe: 0.765 ± 0.121
1.071HisGly: 1.071 ± 0.151
0.459HisHis: 0.459 ± 0.098
1.396HisIle: 1.396 ± 0.182
1.109HisLys: 1.109 ± 0.162
1.377HisLeu: 1.377 ± 0.162
0.497HisMet: 0.497 ± 0.098
0.765HisAsn: 0.765 ± 0.117
0.88HisPro: 0.88 ± 0.128
0.574HisGln: 0.574 ± 0.103
0.746HisArg: 0.746 ± 0.131
1.014HisSer: 1.014 ± 0.153
1.052HisThr: 1.052 ± 0.154
1.09HisVal: 1.09 ± 0.136
0.23HisTrp: 0.23 ± 0.072
0.631HisTyr: 0.631 ± 0.112
0.0HisXaa: 0.0 ± 0.0
Ile
5.049IleAla: 5.049 ± 0.326
0.746IleCys: 0.746 ± 0.123
4.858IleAsp: 4.858 ± 0.295
5.279IleGlu: 5.279 ± 0.255
2.467IlePhe: 2.467 ± 0.218
4.017IleGly: 4.017 ± 0.25
1.205IleHis: 1.205 ± 0.14
4.839IleIle: 4.839 ± 0.286
6.752IleLys: 6.752 ± 0.348
4.284IleLeu: 4.284 ± 0.272
1.683IleMet: 1.683 ± 0.204
4.973IleAsn: 4.973 ± 0.288
2.639IlePro: 2.639 ± 0.241
2.448IleGln: 2.448 ± 0.18
3.29IleArg: 3.29 ± 0.256
4.973IleSer: 4.973 ± 0.29
4.858IleThr: 4.858 ± 0.339
4.323IleVal: 4.323 ± 0.29
0.708IleTrp: 0.708 ± 0.117
2.429IleTyr: 2.429 ± 0.217
0.0IleXaa: 0.0 ± 0.0
Lys
5.891LysAla: 5.891 ± 0.378
0.669LysCys: 0.669 ± 0.115
4.418LysAsp: 4.418 ± 0.252
5.719LysGlu: 5.719 ± 0.408
3.653LysPhe: 3.653 ± 0.268
4.399LysGly: 4.399 ± 0.28
1.53LysHis: 1.53 ± 0.164
4.992LysIle: 4.992 ± 0.34
4.571LysLys: 4.571 ± 0.389
6.235LysLeu: 6.235 ± 0.434
2.333LysMet: 2.333 ± 0.183
4.208LysAsn: 4.208 ± 0.331
2.2LysPro: 2.2 ± 0.205
2.161LysGln: 2.161 ± 0.223
3.175LysArg: 3.175 ± 0.288
4.361LysSer: 4.361 ± 0.276
4.399LysThr: 4.399 ± 0.28
4.858LysVal: 4.858 ± 0.353
1.301LysTrp: 1.301 ± 0.136
3.271LysTyr: 3.271 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
5.183LeuAla: 5.183 ± 0.35
0.88LeuCys: 0.88 ± 0.118
5.394LeuAsp: 5.394 ± 0.339
5.03LeuGlu: 5.03 ± 0.322
3.385LeuPhe: 3.385 ± 0.278
4.208LeuGly: 4.208 ± 0.272
1.148LeuHis: 1.148 ± 0.156
4.629LeuIle: 4.629 ± 0.307
5.968LeuLys: 5.968 ± 0.348
5.088LeuLeu: 5.088 ± 0.338
2.066LeuMet: 2.066 ± 0.222
5.03LeuAsn: 5.03 ± 0.307
2.907LeuPro: 2.907 ± 0.243
2.812LeuGln: 2.812 ± 0.256
3.596LeuArg: 3.596 ± 0.247
4.533LeuSer: 4.533 ± 0.326
4.724LeuThr: 4.724 ± 0.296
4.801LeuVal: 4.801 ± 0.294
0.765LeuTrp: 0.765 ± 0.111
3.041LeuTyr: 3.041 ± 0.236
0.0LeuXaa: 0.0 ± 0.0
Met
2.161MetAla: 2.161 ± 0.173
0.268MetCys: 0.268 ± 0.066
1.377MetAsp: 1.377 ± 0.17
1.798MetGlu: 1.798 ± 0.225
1.377MetPhe: 1.377 ± 0.144
1.32MetGly: 1.32 ± 0.164
0.325MetHis: 0.325 ± 0.093
1.721MetIle: 1.721 ± 0.192
2.372MetLys: 2.372 ± 0.251
2.314MetLeu: 2.314 ± 0.236
0.937MetMet: 0.937 ± 0.158
1.511MetAsn: 1.511 ± 0.186
0.669MetPro: 0.669 ± 0.116
0.746MetGln: 0.746 ± 0.11
1.033MetArg: 1.033 ± 0.117
1.913MetSer: 1.913 ± 0.188
1.798MetThr: 1.798 ± 0.177
1.588MetVal: 1.588 ± 0.168
0.191MetTrp: 0.191 ± 0.061
1.014MetTyr: 1.014 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.787AsnAla: 3.787 ± 0.265
0.44AsnCys: 0.44 ± 0.117
3.194AsnAsp: 3.194 ± 0.273
4.017AsnGlu: 4.017 ± 0.267
2.907AsnPhe: 2.907 ± 0.219
3.806AsnGly: 3.806 ± 0.32
0.956AsnHis: 0.956 ± 0.143
4.055AsnIle: 4.055 ± 0.269
3.959AsnLys: 3.959 ± 0.307
3.883AsnLeu: 3.883 ± 0.278
1.817AsnMet: 1.817 ± 0.17
3.443AsnAsn: 3.443 ± 0.267
2.525AsnPro: 2.525 ± 0.22
1.492AsnGln: 1.492 ± 0.192
2.372AsnArg: 2.372 ± 0.206
3.634AsnSer: 3.634 ± 0.277
3.137AsnThr: 3.137 ± 0.256
3.405AsnVal: 3.405 ± 0.285
0.689AsnTrp: 0.689 ± 0.126
2.123AsnTyr: 2.123 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
2.219ProAla: 2.219 ± 0.228
0.383ProCys: 0.383 ± 0.077
2.353ProAsp: 2.353 ± 0.214
3.328ProGlu: 3.328 ± 0.265
1.664ProPhe: 1.664 ± 0.186
2.314ProGly: 2.314 ± 0.237
0.516ProHis: 0.516 ± 0.104
2.142ProIle: 2.142 ± 0.173
2.314ProLys: 2.314 ± 0.237
2.353ProLeu: 2.353 ± 0.22
0.689ProMet: 0.689 ± 0.13
2.027ProAsn: 2.027 ± 0.201
1.052ProPro: 1.052 ± 0.158
1.033ProGln: 1.033 ± 0.149
1.358ProArg: 1.358 ± 0.178
2.429ProSer: 2.429 ± 0.208
2.314ProThr: 2.314 ± 0.214
2.716ProVal: 2.716 ± 0.274
0.784ProTrp: 0.784 ± 0.134
1.301ProTyr: 1.301 ± 0.153
0.0ProXaa: 0.0 ± 0.0
Gln
2.467GlnAla: 2.467 ± 0.234
0.363GlnCys: 0.363 ± 0.086
1.76GlnAsp: 1.76 ± 0.188
2.353GlnGlu: 2.353 ± 0.188
1.492GlnPhe: 1.492 ± 0.164
1.913GlnGly: 1.913 ± 0.178
0.593GlnHis: 0.593 ± 0.115
2.869GlnIle: 2.869 ± 0.214
2.372GlnLys: 2.372 ± 0.192
2.697GlnLeu: 2.697 ± 0.22
0.995GlnMet: 0.995 ± 0.15
1.435GlnAsn: 1.435 ± 0.165
1.109GlnPro: 1.109 ± 0.14
0.995GlnGln: 0.995 ± 0.149
1.607GlnArg: 1.607 ± 0.191
1.301GlnSer: 1.301 ± 0.16
1.817GlnThr: 1.817 ± 0.195
2.238GlnVal: 2.238 ± 0.223
0.746GlnTrp: 0.746 ± 0.12
1.473GlnTyr: 1.473 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
2.735ArgAla: 2.735 ± 0.28
0.536ArgCys: 0.536 ± 0.126
2.716ArgAsp: 2.716 ± 0.218
3.347ArgGlu: 3.347 ± 0.294
2.085ArgPhe: 2.085 ± 0.179
2.639ArgGly: 2.639 ± 0.225
0.689ArgHis: 0.689 ± 0.111
3.213ArgIle: 3.213 ± 0.276
3.538ArgLys: 3.538 ± 0.305
3.577ArgLeu: 3.577 ± 0.257
1.148ArgMet: 1.148 ± 0.182
2.123ArgAsn: 2.123 ± 0.179
1.396ArgPro: 1.396 ± 0.168
1.779ArgGln: 1.779 ± 0.218
2.104ArgArg: 2.104 ± 0.206
2.544ArgSer: 2.544 ± 0.219
2.219ArgThr: 2.219 ± 0.193
3.118ArgVal: 3.118 ± 0.253
0.516ArgTrp: 0.516 ± 0.124
1.702ArgTyr: 1.702 ± 0.208
0.0ArgXaa: 0.0 ± 0.0
Ser
3.596SerAla: 3.596 ± 0.306
0.689SerCys: 0.689 ± 0.13
4.017SerAsp: 4.017 ± 0.278
4.208SerGlu: 4.208 ± 0.305
2.754SerPhe: 2.754 ± 0.226
4.189SerGly: 4.189 ± 0.331
1.128SerHis: 1.128 ± 0.152
5.049SerIle: 5.049 ± 0.28
4.667SerLys: 4.667 ± 0.305
4.916SerLeu: 4.916 ± 0.261
1.511SerMet: 1.511 ± 0.177
2.907SerAsn: 2.907 ± 0.236
2.582SerPro: 2.582 ± 0.266
2.085SerGln: 2.085 ± 0.193
2.946SerArg: 2.946 ± 0.264
4.839SerSer: 4.839 ± 0.426
4.15SerThr: 4.15 ± 0.349
4.189SerVal: 4.189 ± 0.264
0.975SerTrp: 0.975 ± 0.148
2.735SerTyr: 2.735 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
4.017ThrAla: 4.017 ± 0.439
0.593ThrCys: 0.593 ± 0.123
3.691ThrAsp: 3.691 ± 0.282
4.38ThrGlu: 4.38 ± 0.33
2.429ThrPhe: 2.429 ± 0.219
4.418ThrGly: 4.418 ± 0.378
1.148ThrHis: 1.148 ± 0.174
4.284ThrIle: 4.284 ± 0.296
4.399ThrLys: 4.399 ± 0.328
4.342ThrLeu: 4.342 ± 0.369
1.148ThrMet: 1.148 ± 0.166
2.965ThrAsn: 2.965 ± 0.24
2.754ThrPro: 2.754 ± 0.253
1.798ThrGln: 1.798 ± 0.272
2.716ThrArg: 2.716 ± 0.254
3.558ThrSer: 3.558 ± 0.369
3.806ThrThr: 3.806 ± 0.305
4.82ThrVal: 4.82 ± 0.437
0.861ThrTrp: 0.861 ± 0.13
2.735ThrTyr: 2.735 ± 0.225
0.0ThrXaa: 0.0 ± 0.0
Val
4.265ValAla: 4.265 ± 0.287
0.842ValCys: 0.842 ± 0.129
4.418ValAsp: 4.418 ± 0.308
5.394ValGlu: 5.394 ± 0.301
2.678ValPhe: 2.678 ± 0.228
3.577ValGly: 3.577 ± 0.331
1.205ValHis: 1.205 ± 0.145
4.667ValIle: 4.667 ± 0.321
5.547ValLys: 5.547 ± 0.312
4.59ValLeu: 4.59 ± 0.267
1.721ValMet: 1.721 ± 0.193
3.844ValAsn: 3.844 ± 0.314
2.257ValPro: 2.257 ± 0.199
2.391ValGln: 2.391 ± 0.222
3.079ValArg: 3.079 ± 0.208
4.533ValSer: 4.533 ± 0.302
3.883ValThr: 3.883 ± 0.249
4.629ValVal: 4.629 ± 0.341
0.842ValTrp: 0.842 ± 0.115
2.697ValTyr: 2.697 ± 0.212
0.0ValXaa: 0.0 ± 0.0
Trp
0.842TrpAla: 0.842 ± 0.112
0.134TrpCys: 0.134 ± 0.052
0.803TrpAsp: 0.803 ± 0.135
0.727TrpGlu: 0.727 ± 0.112
0.708TrpPhe: 0.708 ± 0.129
0.574TrpGly: 0.574 ± 0.114
0.172TrpHis: 0.172 ± 0.059
0.956TrpIle: 0.956 ± 0.146
1.473TrpLys: 1.473 ± 0.168
0.88TrpLeu: 0.88 ± 0.132
0.516TrpMet: 0.516 ± 0.104
0.995TrpAsn: 0.995 ± 0.118
0.44TrpPro: 0.44 ± 0.112
0.478TrpGln: 0.478 ± 0.09
0.421TrpArg: 0.421 ± 0.077
0.995TrpSer: 0.995 ± 0.139
0.956TrpThr: 0.956 ± 0.129
0.975TrpVal: 0.975 ± 0.131
0.172TrpTrp: 0.172 ± 0.057
0.765TrpTyr: 0.765 ± 0.113
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.831TyrAla: 2.831 ± 0.227
0.574TyrCys: 0.574 ± 0.11
2.812TyrAsp: 2.812 ± 0.22
2.754TyrGlu: 2.754 ± 0.27
1.913TyrPhe: 1.913 ± 0.241
2.563TyrGly: 2.563 ± 0.242
0.918TyrHis: 0.918 ± 0.134
2.716TyrIle: 2.716 ± 0.242
2.907TyrLys: 2.907 ± 0.249
2.869TyrLeu: 2.869 ± 0.253
1.205TyrMet: 1.205 ± 0.151
2.582TyrAsn: 2.582 ± 0.205
1.664TyrPro: 1.664 ± 0.195
1.473TyrGln: 1.473 ± 0.145
1.626TyrArg: 1.626 ± 0.178
3.003TyrSer: 3.003 ± 0.244
2.563TyrThr: 2.563 ± 0.252
3.175TyrVal: 3.175 ± 0.255
0.631TyrTrp: 0.631 ± 0.105
1.836TyrTyr: 1.836 ± 0.243
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 273 proteins (52284 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski