Amino acid dipepetide frequency for Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	27.058AlaAla: 27.058 ± 0.271	1.97AlaCys: 1.97 ± 0.027	7.1AlaAsp: 7.1 ± 0.053	8.281AlaGlu: 8.281 ± 0.104	4.324AlaPhe: 4.324 ± 0.038	9.09AlaGly: 9.09 ± 0.077	2.379AlaHis: 2.379 ± 0.024	3.976AlaIle: 3.976 ± 0.035	6.068AlaLys: 6.068 ± 0.068	11.309AlaLeu: 11.309 ± 0.075	3.029AlaMet: 3.029 ± 0.027	3.326AlaAsn: 3.326 ± 0.033	7.288AlaPro: 7.288 ± 0.077	2.419AlaGln: 2.419 ± 0.031	12.561AlaArg: 12.561 ± 0.11	11.324AlaSer: 11.324 ± 0.087	8.305AlaThr: 8.305 ± 0.063	8.334AlaVal: 8.334 ± 0.051	1.598AlaTrp: 1.598 ± 0.024	2.195AlaTyr: 2.195 ± 0.026	0.001AlaXaa: 0.001 ± 0.001
Cys	2.069CysAla: 2.069 ± 0.028	0.28CysCys: 0.28 ± 0.01	0.995CysAsp: 0.995 ± 0.019	0.946CysGlu: 0.946 ± 0.018	0.494CysPhe: 0.494 ± 0.012	1.359CysGly: 1.359 ± 0.02	0.301CysHis: 0.301 ± 0.01	0.391CysIle: 0.391 ± 0.011	0.508CysLys: 0.508 ± 0.012	1.072CysLeu: 1.072 ± 0.02	0.248CysMet: 0.248 ± 0.007	0.323CysAsn: 0.323 ± 0.01	0.71CysPro: 0.71 ± 0.019	0.301CysGln: 0.301 ± 0.009	0.877CysArg: 0.877 ± 0.015	0.743CysSer: 0.743 ± 0.015	0.619CysThr: 0.619 ± 0.015	1.586CysVal: 1.586 ± 0.022	0.165CysTrp: 0.165 ± 0.007	0.32CysTyr: 0.32 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	13.014AspAla: 13.014 ± 0.104	0.599AspCys: 0.599 ± 0.011	9.256AspAsp: 9.256 ± 0.115	5.064AspGlu: 5.064 ± 0.047	1.814AspPhe: 1.814 ± 0.021	6.808AspGly: 6.808 ± 0.057	0.926AspHis: 0.926 ± 0.016	1.626AspIle: 1.626 ± 0.024	1.792AspLys: 1.792 ± 0.027	4.127AspLeu: 4.127 ± 0.037	0.934AspMet: 0.934 ± 0.015	1.014AspAsn: 1.014 ± 0.018	3.197AspPro: 3.197 ± 0.03	0.904AspGln: 0.904 ± 0.016	3.523AspArg: 3.523 ± 0.038	2.506AspSer: 2.506 ± 0.029	1.852AspThr: 1.852 ± 0.026	6.087AspVal: 6.087 ± 0.045	0.688AspTrp: 0.688 ± 0.014	1.049AspTyr: 1.049 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	7.567GluAla: 7.567 ± 0.09	0.905GluCys: 0.905 ± 0.017	4.694GluAsp: 4.694 ± 0.042	6.669GluGlu: 6.669 ± 0.091	1.613GluPhe: 1.613 ± 0.02	4.354GluGly: 4.354 ± 0.029	1.225GluHis: 1.225 ± 0.019	2.417GluIle: 2.417 ± 0.025	3.786GluLys: 3.786 ± 0.052	5.39GluLeu: 5.39 ± 0.047	1.548GluMet: 1.548 ± 0.022	1.832GluAsn: 1.832 ± 0.02	2.458GluPro: 2.458 ± 0.034	1.363GluGln: 1.363 ± 0.021	6.139GluArg: 6.139 ± 0.062	3.382GluSer: 3.382 ± 0.035	3.729GluThr: 3.729 ± 0.031	3.708GluVal: 3.708 ± 0.032	0.875GluTrp: 0.875 ± 0.016	1.334GluTyr: 1.334 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	3.914PheAla: 3.914 ± 0.035	0.487PheCys: 0.487 ± 0.013	2.278PheAsp: 2.278 ± 0.022	2.037PheGlu: 2.037 ± 0.025	1.262PhePhe: 1.262 ± 0.023	2.659PheGly: 2.659 ± 0.033	0.685PheHis: 0.685 ± 0.012	1.015PheIle: 1.015 ± 0.016	1.258PheLys: 1.258 ± 0.018	2.494PheLeu: 2.494 ± 0.034	0.592PheMet: 0.592 ± 0.011	1.015PheAsn: 1.015 ± 0.019	1.447PhePro: 1.447 ± 0.018	0.795PheGln: 0.795 ± 0.014	1.814PheArg: 1.814 ± 0.02	1.956PheSer: 1.956 ± 0.023	1.633PheThr: 1.633 ± 0.023	2.509PheVal: 2.509 ± 0.029	0.349PheTrp: 0.349 ± 0.009	0.744PheTyr: 0.744 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	10.701GlyAla: 10.701 ± 0.087	1.176GlyCys: 1.176 ± 0.022	6.452GlyAsp: 6.452 ± 0.048	5.216GlyGlu: 5.216 ± 0.043	2.564GlyPhe: 2.564 ± 0.028	15.91GlyGly: 15.91 ± 0.174	1.443GlyHis: 1.443 ± 0.023	2.236GlyIle: 2.236 ± 0.024	3.565GlyLys: 3.565 ± 0.037	5.065GlyLeu: 5.065 ± 0.039	1.623GlyMet: 1.623 ± 0.024	1.881GlyAsn: 1.881 ± 0.021	2.975GlyPro: 2.975 ± 0.032	1.377GlyGln: 1.377 ± 0.019	5.552GlyArg: 5.552 ± 0.047	5.013GlySer: 5.013 ± 0.047	3.62GlyThr: 3.62 ± 0.038	6.826GlyVal: 6.826 ± 0.055	1.094GlyTrp: 1.094 ± 0.018	1.61GlyTyr: 1.61 ± 0.023	0.0GlyXaa: 0.0 ± 0.0
His	2.8HisAla: 2.8 ± 0.027	0.302HisCys: 0.302 ± 0.009	1.211HisAsp: 1.211 ± 0.016	1.115HisGlu: 1.115 ± 0.015	0.657HisPhe: 0.657 ± 0.013	1.702HisGly: 1.702 ± 0.024	0.668HisHis: 0.668 ± 0.017	0.48HisIle: 0.48 ± 0.012	0.604HisLys: 0.604 ± 0.013	1.716HisLeu: 1.716 ± 0.022	0.311HisMet: 0.311 ± 0.009	0.408HisAsn: 0.408 ± 0.01	1.24HisPro: 1.24 ± 0.02	0.477HisGln: 0.477 ± 0.011	1.519HisArg: 1.519 ± 0.02	0.952HisSer: 0.952 ± 0.018	0.82HisThr: 0.82 ± 0.014	1.719HisVal: 1.719 ± 0.021	0.384HisTrp: 0.384 ± 0.009	0.427HisTyr: 0.427 ± 0.01	0.0HisXaa: 0.0 ± 0.0
Ile	4.316IleAla: 4.316 ± 0.033	0.416IleCys: 0.416 ± 0.01	2.18IleAsp: 2.18 ± 0.023	2.149IleGlu: 2.149 ± 0.027	0.981IlePhe: 0.981 ± 0.016	2.175IleGly: 2.175 ± 0.023	0.652IleHis: 0.652 ± 0.012	1.011IleIle: 1.011 ± 0.019	1.288IleLys: 1.288 ± 0.021	2.287IleLeu: 2.287 ± 0.028	0.593IleMet: 0.593 ± 0.012	0.819IleAsn: 0.819 ± 0.015	1.572IlePro: 1.572 ± 0.023	0.747IleGln: 0.747 ± 0.015	1.926IleArg: 1.926 ± 0.022	1.896IleSer: 1.896 ± 0.028	1.535IleThr: 1.535 ± 0.021	2.496IleVal: 2.496 ± 0.028	0.261IleTrp: 0.261 ± 0.008	0.576IleTyr: 0.576 ± 0.012	0.0IleXaa: 0.0 ± 0.0
Lys	4.857LysAla: 4.857 ± 0.061	0.592LysCys: 0.592 ± 0.013	2.311LysAsp: 2.311 ± 0.028	3.003LysGlu: 3.003 ± 0.043	1.221LysPhe: 1.221 ± 0.017	2.677LysGly: 2.677 ± 0.033	0.952LysHis: 0.952 ± 0.014	1.652LysIle: 1.652 ± 0.024	3.775LysLys: 3.775 ± 0.052	3.564LysLeu: 3.564 ± 0.043	1.047LysMet: 1.047 ± 0.017	1.41LysAsn: 1.41 ± 0.02	2.19LysPro: 2.19 ± 0.027	1.131LysGln: 1.131 ± 0.019	3.722LysArg: 3.722 ± 0.037	2.629LysSer: 2.629 ± 0.027	2.793LysThr: 2.793 ± 0.033	2.494LysVal: 2.494 ± 0.027	0.529LysTrp: 0.529 ± 0.014	0.99LysTyr: 0.99 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	9.963LeuAla: 9.963 ± 0.067	1.316LeuCys: 1.316 ± 0.019	4.668LeuAsp: 4.668 ± 0.041	5.184LeuGlu: 5.184 ± 0.051	2.433LeuPhe: 2.433 ± 0.032	5.681LeuGly: 5.681 ± 0.048	1.708LeuHis: 1.708 ± 0.02	2.145LeuIle: 2.145 ± 0.027	3.49LeuLys: 3.49 ± 0.037	6.763LeuLeu: 6.763 ± 0.061	1.503LeuMet: 1.503 ± 0.021	2.078LeuAsn: 2.078 ± 0.026	4.074LeuPro: 4.074 ± 0.036	1.981LeuGln: 1.981 ± 0.028	7.005LeuArg: 7.005 ± 0.052	5.255LeuSer: 5.255 ± 0.042	4.169LeuThr: 4.169 ± 0.035	5.246LeuVal: 5.246 ± 0.042	0.83LeuTrp: 0.83 ± 0.016	1.579LeuTyr: 1.579 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	2.089MetAla: 2.089 ± 0.025	0.326MetCys: 0.326 ± 0.008	1.201MetAsp: 1.201 ± 0.016	1.259MetGlu: 1.259 ± 0.019	0.669MetPhe: 0.669 ± 0.016	1.38MetGly: 1.38 ± 0.023	0.461MetHis: 0.461 ± 0.01	0.694MetIle: 0.694 ± 0.014	1.076MetLys: 1.076 ± 0.019	1.62MetLeu: 1.62 ± 0.022	0.589MetMet: 0.589 ± 0.014	0.662MetAsn: 0.662 ± 0.014	1.067MetPro: 1.067 ± 0.016	0.585MetGln: 0.585 ± 0.012	1.751MetArg: 1.751 ± 0.017	1.456MetSer: 1.456 ± 0.018	1.386MetThr: 1.386 ± 0.019	0.965MetVal: 0.965 ± 0.017	0.213MetTrp: 0.213 ± 0.007	0.456MetTyr: 0.456 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	4.098AsnAla: 4.098 ± 0.034	0.326AsnCys: 0.326 ± 0.009	1.701AsnAsp: 1.701 ± 0.024	1.505AsnGlu: 1.505 ± 0.023	0.868AsnPhe: 0.868 ± 0.017	2.169AsnGly: 2.169 ± 0.024	0.488AsnHis: 0.488 ± 0.01	0.859AsnIle: 0.859 ± 0.017	1.05AsnLys: 1.05 ± 0.017	1.938AsnLeu: 1.938 ± 0.027	0.501AsnMet: 0.501 ± 0.014	0.707AsnAsn: 0.707 ± 0.012	1.51AsnPro: 1.51 ± 0.022	0.566AsnGln: 0.566 ± 0.013	1.384AsnArg: 1.384 ± 0.019	1.247AsnSer: 1.247 ± 0.018	1.066AsnThr: 1.066 ± 0.018	2.129AsnVal: 2.129 ± 0.025	0.274AsnTrp: 0.274 ± 0.008	0.556AsnTyr: 0.556 ± 0.011	0.0AsnXaa: 0.0 ± 0.0
Pro	6.44ProAla: 6.44 ± 0.07	0.61ProCys: 0.61 ± 0.014	2.649ProAsp: 2.649 ± 0.024	3.177ProGlu: 3.177 ± 0.042	1.624ProPhe: 1.624 ± 0.02	4.465ProGly: 4.465 ± 0.049	1.005ProHis: 1.005 ± 0.016	1.511ProIle: 1.511 ± 0.02	2.052ProLys: 2.052 ± 0.029	3.606ProLeu: 3.606 ± 0.035	0.951ProMet: 0.951 ± 0.017	1.309ProAsn: 1.309 ± 0.017	7.092ProPro: 7.092 ± 0.115	0.936ProGln: 0.936 ± 0.018	4.667ProArg: 4.667 ± 0.045	4.792ProSer: 4.792 ± 0.043	3.309ProThr: 3.309 ± 0.034	2.899ProVal: 2.899 ± 0.031	0.691ProTrp: 0.691 ± 0.018	1.019ProTyr: 1.019 ± 0.014	0.0ProXaa: 0.0 ± 0.0
Gln	2.008GlnAla: 2.008 ± 0.026	0.367GlnCys: 0.367 ± 0.009	0.875GlnAsp: 0.875 ± 0.015	1.098GlnGlu: 1.098 ± 0.016	0.661GlnPhe: 0.661 ± 0.015	1.307GlnGly: 1.307 ± 0.02	0.502GlnHis: 0.502 ± 0.01	0.896GlnIle: 0.896 ± 0.016	1.095GlnLys: 1.095 ± 0.02	2.043GlnLeu: 2.043 ± 0.026	0.579GlnMet: 0.579 ± 0.014	0.64GlnAsn: 0.64 ± 0.014	1.02GlnPro: 1.02 ± 0.017	0.98GlnGln: 0.98 ± 0.036	1.959GlnArg: 1.959 ± 0.026	1.297GlnSer: 1.297 ± 0.017	1.213GlnThr: 1.213 ± 0.017	1.284GlnVal: 1.284 ± 0.018	0.354GlnTrp: 0.354 ± 0.015	0.513GlnTyr: 0.513 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	12.851ArgAla: 12.851 ± 0.111	1.24ArgCys: 1.24 ± 0.018	5.249ArgAsp: 5.249 ± 0.051	5.879ArgGlu: 5.879 ± 0.058	2.153ArgPhe: 2.153 ± 0.025	7.316ArgGly: 7.316 ± 0.062	1.481ArgHis: 1.481 ± 0.02	1.872ArgIle: 1.872 ± 0.02	2.969ArgLys: 2.969 ± 0.033	5.79ArgLeu: 5.79 ± 0.045	1.441ArgMet: 1.441 ± 0.021	1.538ArgAsn: 1.538 ± 0.02	3.558ArgPro: 3.558 ± 0.038	1.524ArgGln: 1.524 ± 0.019	9.592ArgArg: 9.592 ± 0.109	4.726ArgSer: 4.726 ± 0.044	3.3ArgThr: 3.3 ± 0.028	6.268ArgVal: 6.268 ± 0.051	1.186ArgTrp: 1.186 ± 0.021	1.352ArgTyr: 1.352 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	8.424SerAla: 8.424 ± 0.061	0.86SerCys: 0.86 ± 0.016	4.006SerAsp: 4.006 ± 0.034	3.453SerGlu: 3.453 ± 0.033	2.157SerPhe: 2.157 ± 0.025	5.306SerGly: 5.306 ± 0.049	1.33SerHis: 1.33 ± 0.019	2.107SerIle: 2.107 ± 0.027	2.671SerLys: 2.671 ± 0.031	5.267SerLeu: 5.267 ± 0.042	1.215SerMet: 1.215 ± 0.02	1.614SerAsn: 1.614 ± 0.02	4.563SerPro: 4.563 ± 0.049	1.281SerGln: 1.281 ± 0.017	5.225SerArg: 5.225 ± 0.047	7.98SerSer: 7.98 ± 0.105	3.763SerThr: 3.763 ± 0.04	4.224SerVal: 4.224 ± 0.034	0.728SerTrp: 0.728 ± 0.012	1.228SerTyr: 1.228 ± 0.017	0.0SerXaa: 0.0 ± 0.0
Thr	6.379ThrAla: 6.379 ± 0.05	0.882ThrCys: 0.882 ± 0.02	2.335ThrAsp: 2.335 ± 0.026	2.606ThrGlu: 2.606 ± 0.026	1.864ThrPhe: 1.864 ± 0.025	3.626ThrGly: 3.626 ± 0.047	1.162ThrHis: 1.162 ± 0.016	1.837ThrIle: 1.837 ± 0.02	2.164ThrLys: 2.164 ± 0.024	4.631ThrLeu: 4.631 ± 0.029	1.09ThrMet: 1.09 ± 0.019	1.291ThrAsn: 1.291 ± 0.019	4.078ThrPro: 4.078 ± 0.041	1.136ThrGln: 1.136 ± 0.019	4.66ThrArg: 4.66 ± 0.038	3.937ThrSer: 3.937 ± 0.04	4.88ThrThr: 4.88 ± 0.067	3.075ThrVal: 3.075 ± 0.036	0.651ThrTrp: 0.651 ± 0.012	1.128ThrTyr: 1.128 ± 0.017	0.0ThrXaa: 0.0 ± 0.0
Val	9.942ValAla: 9.942 ± 0.071	1.071ValCys: 1.071 ± 0.017	5.102ValAsp: 5.102 ± 0.036	4.379ValGlu: 4.379 ± 0.035	2.394ValPhe: 2.394 ± 0.028	5.425ValGly: 5.425 ± 0.047	1.408ValHis: 1.408 ± 0.02	2.196ValIle: 2.196 ± 0.025	2.973ValLys: 2.973 ± 0.031	5.707ValLeu: 5.707 ± 0.049	1.429ValMet: 1.429 ± 0.018	2.056ValAsn: 2.056 ± 0.02	3.449ValPro: 3.449 ± 0.032	1.388ValGln: 1.388 ± 0.02	4.993ValArg: 4.993 ± 0.034	4.398ValSer: 4.398 ± 0.038	3.701ValThr: 3.701 ± 0.042	6.992ValVal: 6.992 ± 0.053	0.747ValTrp: 0.747 ± 0.015	1.45ValTyr: 1.45 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	1.106TrpAla: 1.106 ± 0.024	0.256TrpCys: 0.256 ± 0.008	0.815TrpAsp: 0.815 ± 0.019	0.688TrpGlu: 0.688 ± 0.013	0.439TrpPhe: 0.439 ± 0.012	0.845TrpGly: 0.845 ± 0.018	0.286TrpHis: 0.286 ± 0.01	0.449TrpIle: 0.449 ± 0.01	0.59TrpLys: 0.59 ± 0.012	1.013TrpLeu: 1.013 ± 0.017	0.319TrpMet: 0.319 ± 0.009	0.451TrpAsn: 0.451 ± 0.01	0.446TrpPro: 0.446 ± 0.009	0.29TrpGln: 0.29 ± 0.008	1.199TrpArg: 1.199 ± 0.022	0.913TrpSer: 0.913 ± 0.015	0.809TrpThr: 0.809 ± 0.014	0.657TrpVal: 0.657 ± 0.012	0.205TrpTrp: 0.205 ± 0.006	0.301TrpTyr: 0.301 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.337TyrAla: 2.337 ± 0.024	0.327TyrCys: 0.327 ± 0.007	1.697TyrAsp: 1.697 ± 0.021	1.28TyrGlu: 1.28 ± 0.021	0.751TyrPhe: 0.751 ± 0.013	1.646TyrGly: 1.646 ± 0.025	0.452TyrHis: 0.452 ± 0.011	0.501TyrIle: 0.501 ± 0.01	0.766TyrLys: 0.766 ± 0.015	1.67TyrLeu: 1.67 ± 0.023	0.363TyrMet: 0.363 ± 0.011	0.569TyrAsn: 0.569 ± 0.013	0.872TyrPro: 0.872 ± 0.014	0.511TyrGln: 0.511 ± 0.013	1.218TyrArg: 1.218 ± 0.017	0.999TyrSer: 0.999 ± 0.014	0.908TyrThr: 0.908 ± 0.015	1.706TyrVal: 1.706 ± 0.022	0.253TyrTrp: 0.253 ± 0.008	0.595TyrTyr: 0.595 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 10250 proteins (4600988 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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