Mycobacterium phage SWU1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; Mycobacterium virus SWU1

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1V1M4|I1V1M4_9CAUD Uncharacterized protein OS=Mycobacterium phage SWU1 OX=1175504 PE=4 SV=1
MM1 pKa = 7.33PAKK4 pKa = 10.15NFRR7 pKa = 11.84RR8 pKa = 11.84IPDD11 pKa = 3.32QTDD14 pKa = 3.16DD15 pKa = 3.65QGFLIPGYY23 pKa = 8.72SAWDD27 pKa = 3.61CQRR30 pKa = 11.84CGGEE34 pKa = 3.71VCRR37 pKa = 11.84YY38 pKa = 9.01YY39 pKa = 11.06GQSDD43 pKa = 4.01VDD45 pKa = 4.21CPNCGACYY53 pKa = 9.59NASGQRR59 pKa = 11.84LRR61 pKa = 11.84DD62 pKa = 3.55DD63 pKa = 3.04WRR65 pKa = 11.84GNPSAYY71 pKa = 10.1DD72 pKa = 3.7DD73 pKa = 3.85EE74 pKa = 6.22VGDD77 pKa = 4.01LEE79 pKa = 5.18GFEE82 pKa = 4.61TQHH85 pKa = 6.96SDD87 pKa = 3.36YY88 pKa = 11.76

Molecular weight: 9.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1V1G7|I1V1G7_9CAUD MazG domain-containing protein OS=Mycobacterium phage SWU1 OX=1175504 PE=4 SV=1
MM1 pKa = 7.9PPRR4 pKa = 11.84ASIQEE9 pKa = 4.04TADD12 pKa = 3.51YY13 pKa = 10.85LGVSTKK19 pKa = 7.22TVRR22 pKa = 11.84NYY24 pKa = 10.21IADD27 pKa = 3.64GRR29 pKa = 11.84LKK31 pKa = 10.45AVRR34 pKa = 11.84LGPRR38 pKa = 11.84LIRR41 pKa = 11.84VEE43 pKa = 4.05RR44 pKa = 11.84DD45 pKa = 3.15SVEE48 pKa = 3.78ALMRR52 pKa = 11.84PIGKK56 pKa = 9.51

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

15751

28

837

167.6

18.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.676 ± 0.354

0.908 ± 0.114

6.368 ± 0.259

7.066 ± 0.286

3.39 ± 0.151

8.114 ± 0.394

2.209 ± 0.187

5.066 ± 0.161

4.946 ± 0.255

8.019 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.571 ± 0.125

3.295 ± 0.171

5.174 ± 0.214

3.619 ± 0.234

6.419 ± 0.368

5.079 ± 0.219

5.892 ± 0.279

7.142 ± 0.207

2.019 ± 0.153

3.028 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski