Amino acid dipepetide frequency for Mycobacterium phage SWU1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.413AlaAla: 10.413 ± 0.941
0.698AlaCys: 0.698 ± 0.222
5.714AlaAsp: 5.714 ± 0.709
7.746AlaGlu: 7.746 ± 0.73
3.619AlaPhe: 3.619 ± 0.535
7.873AlaGly: 7.873 ± 0.927
1.587AlaHis: 1.587 ± 0.322
5.079AlaIle: 5.079 ± 0.536
5.016AlaLys: 5.016 ± 0.464
8.889AlaLeu: 8.889 ± 1.033
2.54AlaMet: 2.54 ± 0.348
2.921AlaAsn: 2.921 ± 0.432
4.317AlaPro: 4.317 ± 0.687
3.937AlaGln: 3.937 ± 0.618
5.333AlaArg: 5.333 ± 0.553
4.063AlaSer: 4.063 ± 0.489
5.46AlaThr: 5.46 ± 0.604
6.667AlaVal: 6.667 ± 0.611
2.095AlaTrp: 2.095 ± 0.305
2.73AlaTyr: 2.73 ± 0.497
0.0AlaXaa: 0.0 ± 0.0
Cys
0.635CysAla: 0.635 ± 0.211
0.063CysCys: 0.063 ± 0.064
0.635CysAsp: 0.635 ± 0.199
0.571CysGlu: 0.571 ± 0.167
0.381CysPhe: 0.381 ± 0.161
0.762CysGly: 0.762 ± 0.227
0.19CysHis: 0.19 ± 0.108
0.317CysIle: 0.317 ± 0.154
0.571CysLys: 0.571 ± 0.163
0.825CysLeu: 0.825 ± 0.208
0.381CysMet: 0.381 ± 0.181
0.381CysAsn: 0.381 ± 0.127
0.571CysPro: 0.571 ± 0.21
0.317CysGln: 0.317 ± 0.127
0.571CysArg: 0.571 ± 0.163
0.381CysSer: 0.381 ± 0.186
0.508CysThr: 0.508 ± 0.155
0.444CysVal: 0.444 ± 0.148
0.317CysTrp: 0.317 ± 0.166
0.254CysTyr: 0.254 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
5.778AspAla: 5.778 ± 0.656
0.698AspCys: 0.698 ± 0.249
3.937AspAsp: 3.937 ± 0.66
4.952AspGlu: 4.952 ± 0.698
2.286AspPhe: 2.286 ± 0.413
5.968AspGly: 5.968 ± 0.682
1.397AspHis: 1.397 ± 0.311
3.175AspIle: 3.175 ± 0.394
2.54AspLys: 2.54 ± 0.385
4.889AspLeu: 4.889 ± 0.56
1.46AspMet: 1.46 ± 0.295
1.397AspAsn: 1.397 ± 0.319
4.127AspPro: 4.127 ± 0.531
2.54AspGln: 2.54 ± 0.532
3.683AspArg: 3.683 ± 0.421
3.048AspSer: 3.048 ± 0.498
3.683AspThr: 3.683 ± 0.463
4.317AspVal: 4.317 ± 0.477
1.333AspTrp: 1.333 ± 0.264
2.476AspTyr: 2.476 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
6.984GluAla: 6.984 ± 0.868
0.317GluCys: 0.317 ± 0.12
4.444GluAsp: 4.444 ± 0.498
5.016GluGlu: 5.016 ± 0.688
3.048GluPhe: 3.048 ± 0.443
5.333GluGly: 5.333 ± 0.621
1.206GluHis: 1.206 ± 0.289
3.746GluIle: 3.746 ± 0.505
2.54GluLys: 2.54 ± 0.353
7.81GluLeu: 7.81 ± 0.751
2.54GluMet: 2.54 ± 0.386
2.603GluAsn: 2.603 ± 0.326
2.476GluPro: 2.476 ± 0.411
1.714GluGln: 1.714 ± 0.252
5.206GluArg: 5.206 ± 0.562
2.667GluSer: 2.667 ± 0.447
3.81GluThr: 3.81 ± 0.497
5.333GluVal: 5.333 ± 0.681
1.206GluTrp: 1.206 ± 0.278
2.667GluTyr: 2.667 ± 0.359
0.0GluXaa: 0.0 ± 0.0
Phe
3.302PheAla: 3.302 ± 0.517
0.381PheCys: 0.381 ± 0.144
2.159PheAsp: 2.159 ± 0.434
3.302PheGlu: 3.302 ± 0.439
0.698PhePhe: 0.698 ± 0.217
2.794PheGly: 2.794 ± 0.3
0.825PheHis: 0.825 ± 0.282
1.651PheIle: 1.651 ± 0.426
1.397PheLys: 1.397 ± 0.317
2.286PheLeu: 2.286 ± 0.403
0.508PheMet: 0.508 ± 0.207
1.206PheAsn: 1.206 ± 0.244
2.032PhePro: 2.032 ± 0.357
1.397PheGln: 1.397 ± 0.351
2.222PheArg: 2.222 ± 0.352
2.032PheSer: 2.032 ± 0.326
2.476PheThr: 2.476 ± 0.326
1.968PheVal: 1.968 ± 0.347
0.571PheTrp: 0.571 ± 0.193
0.698PheTyr: 0.698 ± 0.198
0.0PheXaa: 0.0 ± 0.0
Gly
6.921GlyAla: 6.921 ± 0.892
0.698GlyCys: 0.698 ± 0.226
5.524GlyAsp: 5.524 ± 0.61
4.825GlyGlu: 4.825 ± 0.622
3.111GlyPhe: 3.111 ± 0.497
7.937GlyGly: 7.937 ± 1.246
1.968GlyHis: 1.968 ± 0.33
4.317GlyIle: 4.317 ± 0.658
3.873GlyLys: 3.873 ± 0.46
6.54GlyLeu: 6.54 ± 0.821
2.032GlyMet: 2.032 ± 0.432
3.429GlyAsn: 3.429 ± 0.402
3.048GlyPro: 3.048 ± 0.369
3.048GlyGln: 3.048 ± 0.496
4.444GlyArg: 4.444 ± 0.548
3.556GlySer: 3.556 ± 0.554
4.952GlyThr: 4.952 ± 0.708
6.349GlyVal: 6.349 ± 0.721
1.905GlyTrp: 1.905 ± 0.316
2.667GlyTyr: 2.667 ± 0.352
0.0GlyXaa: 0.0 ± 0.0
His
1.714HisAla: 1.714 ± 0.366
0.381HisCys: 0.381 ± 0.145
1.524HisAsp: 1.524 ± 0.305
1.587HisGlu: 1.587 ± 0.338
0.381HisPhe: 0.381 ± 0.139
1.587HisGly: 1.587 ± 0.315
0.571HisHis: 0.571 ± 0.191
1.587HisIle: 1.587 ± 0.329
1.206HisLys: 1.206 ± 0.283
1.651HisLeu: 1.651 ± 0.329
0.444HisMet: 0.444 ± 0.174
0.444HisAsn: 0.444 ± 0.18
1.333HisPro: 1.333 ± 0.26
0.825HisGln: 0.825 ± 0.186
1.968HisArg: 1.968 ± 0.453
0.825HisSer: 0.825 ± 0.24
1.46HisThr: 1.46 ± 0.392
1.397HisVal: 1.397 ± 0.26
0.381HisTrp: 0.381 ± 0.155
0.825HisTyr: 0.825 ± 0.32
0.0HisXaa: 0.0 ± 0.0
Ile
5.968IleAla: 5.968 ± 0.573
0.444IleCys: 0.444 ± 0.16
3.683IleAsp: 3.683 ± 0.452
4.444IleGlu: 4.444 ± 0.453
1.333IlePhe: 1.333 ± 0.269
3.619IleGly: 3.619 ± 0.537
1.397IleHis: 1.397 ± 0.351
1.778IleIle: 1.778 ± 0.321
2.984IleLys: 2.984 ± 0.443
4.19IleLeu: 4.19 ± 0.538
0.762IleMet: 0.762 ± 0.186
1.778IleAsn: 1.778 ± 0.316
3.683IlePro: 3.683 ± 0.407
1.778IleGln: 1.778 ± 0.334
2.921IleArg: 2.921 ± 0.412
2.667IleSer: 2.667 ± 0.417
2.603IleThr: 2.603 ± 0.355
2.984IleVal: 2.984 ± 0.397
0.635IleTrp: 0.635 ± 0.214
1.016IleTyr: 1.016 ± 0.278
0.0IleXaa: 0.0 ± 0.0
Lys
5.206LysAla: 5.206 ± 0.532
0.254LysCys: 0.254 ± 0.133
3.302LysAsp: 3.302 ± 0.389
2.095LysGlu: 2.095 ± 0.347
1.143LysPhe: 1.143 ± 0.274
3.365LysGly: 3.365 ± 0.448
1.016LysHis: 1.016 ± 0.244
2.286LysIle: 2.286 ± 0.36
3.492LysLys: 3.492 ± 0.508
3.746LysLeu: 3.746 ± 0.469
1.46LysMet: 1.46 ± 0.277
1.206LysAsn: 1.206 ± 0.27
3.556LysPro: 3.556 ± 0.519
1.651LysGln: 1.651 ± 0.35
3.81LysArg: 3.81 ± 0.497
2.222LysSer: 2.222 ± 0.361
2.984LysThr: 2.984 ± 0.412
4.762LysVal: 4.762 ± 0.617
0.825LysTrp: 0.825 ± 0.22
1.079LysTyr: 1.079 ± 0.344
0.0LysXaa: 0.0 ± 0.0
Leu
8.19LeuAla: 8.19 ± 0.791
0.698LeuCys: 0.698 ± 0.234
4.508LeuAsp: 4.508 ± 0.455
6.032LeuGlu: 6.032 ± 0.471
2.54LeuPhe: 2.54 ± 0.348
6.603LeuGly: 6.603 ± 0.793
2.286LeuHis: 2.286 ± 0.392
4.19LeuIle: 4.19 ± 0.498
3.429LeuLys: 3.429 ± 0.455
5.016LeuLeu: 5.016 ± 0.558
2.667LeuMet: 2.667 ± 0.429
2.667LeuAsn: 2.667 ± 0.452
4.317LeuPro: 4.317 ± 0.433
3.111LeuGln: 3.111 ± 0.755
4.698LeuArg: 4.698 ± 0.618
5.587LeuSer: 5.587 ± 0.595
4.571LeuThr: 4.571 ± 0.534
4.889LeuVal: 4.889 ± 0.582
1.651LeuTrp: 1.651 ± 0.267
2.54LeuTyr: 2.54 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
2.73MetAla: 2.73 ± 0.408
0.19MetCys: 0.19 ± 0.109
1.016MetAsp: 1.016 ± 0.225
1.587MetGlu: 1.587 ± 0.266
0.381MetPhe: 0.381 ± 0.132
1.587MetGly: 1.587 ± 0.318
0.381MetHis: 0.381 ± 0.154
1.587MetIle: 1.587 ± 0.306
1.905MetLys: 1.905 ± 0.303
1.46MetLeu: 1.46 ± 0.315
0.444MetMet: 0.444 ± 0.155
0.762MetAsn: 0.762 ± 0.244
1.841MetPro: 1.841 ± 0.322
1.079MetGln: 1.079 ± 0.265
1.841MetArg: 1.841 ± 0.293
2.032MetSer: 2.032 ± 0.279
2.603MetThr: 2.603 ± 0.408
1.587MetVal: 1.587 ± 0.31
0.317MetTrp: 0.317 ± 0.134
0.381MetTyr: 0.381 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
2.857AsnAla: 2.857 ± 0.478
0.444AsnCys: 0.444 ± 0.159
1.714AsnAsp: 1.714 ± 0.366
1.905AsnGlu: 1.905 ± 0.349
0.889AsnPhe: 0.889 ± 0.263
3.302AsnGly: 3.302 ± 0.479
0.889AsnHis: 0.889 ± 0.238
1.333AsnIle: 1.333 ± 0.355
1.27AsnLys: 1.27 ± 0.335
2.794AsnLeu: 2.794 ± 0.391
1.016AsnMet: 1.016 ± 0.236
0.889AsnAsn: 0.889 ± 0.256
2.413AsnPro: 2.413 ± 0.382
1.143AsnGln: 1.143 ± 0.288
2.54AsnArg: 2.54 ± 0.439
1.651AsnSer: 1.651 ± 0.307
1.968AsnThr: 1.968 ± 0.421
2.413AsnVal: 2.413 ± 0.33
0.571AsnTrp: 0.571 ± 0.186
0.952AsnTyr: 0.952 ± 0.182
0.0AsnXaa: 0.0 ± 0.0
Pro
4.381ProAla: 4.381 ± 0.527
0.444ProCys: 0.444 ± 0.185
3.683ProAsp: 3.683 ± 0.463
4.381ProGlu: 4.381 ± 0.559
1.968ProPhe: 1.968 ± 0.376
4.19ProGly: 4.19 ± 0.589
1.206ProHis: 1.206 ± 0.254
3.048ProIle: 3.048 ± 0.333
2.159ProLys: 2.159 ± 0.517
3.111ProLeu: 3.111 ± 0.332
1.397ProMet: 1.397 ± 0.31
1.778ProAsn: 1.778 ± 0.305
2.032ProPro: 2.032 ± 0.473
1.778ProGln: 1.778 ± 0.384
3.175ProArg: 3.175 ± 0.581
2.921ProSer: 2.921 ± 0.391
3.302ProThr: 3.302 ± 0.435
4.19ProVal: 4.19 ± 0.509
1.143ProTrp: 1.143 ± 0.405
1.46ProTyr: 1.46 ± 0.335
0.0ProXaa: 0.0 ± 0.0
Gln
4.381GlnAla: 4.381 ± 0.666
0.317GlnCys: 0.317 ± 0.136
1.524GlnAsp: 1.524 ± 0.328
1.905GlnGlu: 1.905 ± 0.355
1.587GlnPhe: 1.587 ± 0.326
2.794GlnGly: 2.794 ± 0.329
0.889GlnHis: 0.889 ± 0.213
2.286GlnIle: 2.286 ± 0.35
1.968GlnLys: 1.968 ± 0.356
3.429GlnLeu: 3.429 ± 0.606
1.079GlnMet: 1.079 ± 0.275
1.143GlnAsn: 1.143 ± 0.207
1.333GlnPro: 1.333 ± 0.302
1.524GlnGln: 1.524 ± 0.402
2.159GlnArg: 2.159 ± 0.366
1.587GlnSer: 1.587 ± 0.378
1.841GlnThr: 1.841 ± 0.423
2.603GlnVal: 2.603 ± 0.387
0.952GlnTrp: 0.952 ± 0.209
0.889GlnTyr: 0.889 ± 0.204
0.0GlnXaa: 0.0 ± 0.0
Arg
5.524ArgAla: 5.524 ± 0.682
1.016ArgCys: 1.016 ± 0.255
4.635ArgAsp: 4.635 ± 0.602
4.635ArgGlu: 4.635 ± 0.52
2.286ArgPhe: 2.286 ± 0.481
3.683ArgGly: 3.683 ± 0.521
1.333ArgHis: 1.333 ± 0.271
4.381ArgIle: 4.381 ± 0.505
3.873ArgLys: 3.873 ± 0.583
5.841ArgLeu: 5.841 ± 0.577
1.651ArgMet: 1.651 ± 0.305
2.349ArgAsn: 2.349 ± 0.377
2.667ArgPro: 2.667 ± 0.408
1.841ArgGln: 1.841 ± 0.373
5.587ArgArg: 5.587 ± 0.702
2.413ArgSer: 2.413 ± 0.373
2.603ArgThr: 2.603 ± 0.375
4.0ArgVal: 4.0 ± 0.495
1.397ArgTrp: 1.397 ± 0.286
2.476ArgTyr: 2.476 ± 0.403
0.0ArgXaa: 0.0 ± 0.0
Ser
4.571SerAla: 4.571 ± 0.433
0.508SerCys: 0.508 ± 0.184
3.048SerAsp: 3.048 ± 0.441
3.556SerGlu: 3.556 ± 0.472
2.095SerPhe: 2.095 ± 0.354
4.698SerGly: 4.698 ± 0.596
0.825SerHis: 0.825 ± 0.205
2.476SerIle: 2.476 ± 0.311
2.54SerLys: 2.54 ± 0.381
3.81SerLeu: 3.81 ± 0.558
1.206SerMet: 1.206 ± 0.191
1.587SerAsn: 1.587 ± 0.294
2.921SerPro: 2.921 ± 0.445
1.905SerGln: 1.905 ± 0.255
3.937SerArg: 3.937 ± 0.48
2.603SerSer: 2.603 ± 0.524
2.603SerThr: 2.603 ± 0.374
3.302SerVal: 3.302 ± 0.388
1.333SerTrp: 1.333 ± 0.275
1.27SerTyr: 1.27 ± 0.318
0.0SerXaa: 0.0 ± 0.0
Thr
6.286ThrAla: 6.286 ± 0.616
0.508ThrCys: 0.508 ± 0.186
3.873ThrAsp: 3.873 ± 0.527
3.429ThrGlu: 3.429 ± 0.49
2.413ThrPhe: 2.413 ± 0.35
5.016ThrGly: 5.016 ± 0.646
1.27ThrHis: 1.27 ± 0.286
2.54ThrIle: 2.54 ± 0.459
3.365ThrLys: 3.365 ± 0.459
4.508ThrLeu: 4.508 ± 0.584
1.524ThrMet: 1.524 ± 0.26
1.651ThrAsn: 1.651 ± 0.332
3.429ThrPro: 3.429 ± 0.455
2.095ThrGln: 2.095 ± 0.309
2.857ThrArg: 2.857 ± 0.409
3.048ThrSer: 3.048 ± 0.422
2.667ThrThr: 2.667 ± 0.426
5.143ThrVal: 5.143 ± 0.611
1.333ThrTrp: 1.333 ± 0.306
1.968ThrTyr: 1.968 ± 0.335
0.0ThrXaa: 0.0 ± 0.0
Val
6.54ValAla: 6.54 ± 0.923
0.635ValCys: 0.635 ± 0.173
5.27ValAsp: 5.27 ± 0.523
5.143ValGlu: 5.143 ± 0.599
2.032ValPhe: 2.032 ± 0.37
5.587ValGly: 5.587 ± 0.581
1.841ValHis: 1.841 ± 0.339
2.667ValIle: 2.667 ± 0.449
3.365ValLys: 3.365 ± 0.471
5.27ValLeu: 5.27 ± 0.525
1.397ValMet: 1.397 ± 0.29
2.921ValAsn: 2.921 ± 0.529
3.429ValPro: 3.429 ± 0.578
2.032ValGln: 2.032 ± 0.355
4.381ValArg: 4.381 ± 0.425
4.508ValSer: 4.508 ± 0.582
5.333ValThr: 5.333 ± 0.592
5.46ValVal: 5.46 ± 0.499
1.27ValTrp: 1.27 ± 0.301
2.349ValTyr: 2.349 ± 0.478
0.0ValXaa: 0.0 ± 0.0
Trp
1.714TrpAla: 1.714 ± 0.348
0.254TrpCys: 0.254 ± 0.135
1.397TrpAsp: 1.397 ± 0.287
1.587TrpGlu: 1.587 ± 0.295
0.762TrpPhe: 0.762 ± 0.187
1.651TrpGly: 1.651 ± 0.347
0.635TrpHis: 0.635 ± 0.228
1.016TrpIle: 1.016 ± 0.247
0.635TrpLys: 0.635 ± 0.181
1.079TrpLeu: 1.079 ± 0.264
0.381TrpMet: 0.381 ± 0.134
0.635TrpAsn: 0.635 ± 0.207
1.016TrpPro: 1.016 ± 0.288
1.079TrpGln: 1.079 ± 0.246
0.889TrpArg: 0.889 ± 0.213
1.333TrpSer: 1.333 ± 0.268
1.587TrpThr: 1.587 ± 0.315
1.27TrpVal: 1.27 ± 0.318
0.508TrpTrp: 0.508 ± 0.166
0.762TrpTyr: 0.762 ± 0.236
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.667TyrAla: 2.667 ± 0.422
0.127TyrCys: 0.127 ± 0.101
2.095TyrAsp: 2.095 ± 0.321
1.968TyrGlu: 1.968 ± 0.401
0.952TyrPhe: 0.952 ± 0.236
2.794TyrGly: 2.794 ± 0.359
0.381TyrHis: 0.381 ± 0.156
1.206TyrIle: 1.206 ± 0.248
1.333TyrLys: 1.333 ± 0.314
3.048TyrLeu: 3.048 ± 0.448
0.762TyrMet: 0.762 ± 0.212
1.206TyrAsn: 1.206 ± 0.259
1.206TyrPro: 1.206 ± 0.238
1.397TyrGln: 1.397 ± 0.296
1.968TyrArg: 1.968 ± 0.374
1.651TyrSer: 1.651 ± 0.309
1.905TyrThr: 1.905 ± 0.301
2.349TyrVal: 2.349 ± 0.48
0.444TyrTrp: 0.444 ± 0.22
0.825TyrTyr: 0.825 ± 0.253
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (15751 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski