Mycobacterium virus Ardmore

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4N7J5|D4N7J5_9CAUD Gp50 OS=Mycobacterium virus Ardmore OX=685506 GN=Ardmore_50 PE=3 SV=1
MM1 pKa = 7.36AHH3 pKa = 6.42WKK5 pKa = 9.59YY6 pKa = 8.91WWTMPLLIAAGIIGPGLAAPEE27 pKa = 4.19AKK29 pKa = 10.36ADD31 pKa = 3.49ITSDD35 pKa = 3.54AFVMALDD42 pKa = 4.35SEE44 pKa = 5.11GITYY48 pKa = 10.39SSKK51 pKa = 10.3PAVINAGKK59 pKa = 10.01AVCDD63 pKa = 3.93VLDD66 pKa = 3.65TGYY69 pKa = 9.51TMYY72 pKa = 10.19EE73 pKa = 3.7ASVFVYY79 pKa = 10.8NNSNLDD85 pKa = 3.99LYY87 pKa = 11.33DD88 pKa = 3.2SGYY91 pKa = 10.79FVGAATASFCPEE103 pKa = 4.1HH104 pKa = 7.29LSGTGWVV111 pKa = 3.57

Molecular weight: 11.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4N7N3|D4N7N3_9CAUD Gp88 OS=Mycobacterium virus Ardmore OX=685506 GN=Ardmore_88 PE=4 SV=1
MM1 pKa = 7.49GRR3 pKa = 11.84LQRR6 pKa = 11.84TRR8 pKa = 11.84TRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.3RR13 pKa = 11.84RR14 pKa = 11.84EE15 pKa = 3.67NPIQRR20 pKa = 11.84LGQMLHH26 pKa = 5.36RR27 pKa = 11.84HH28 pKa = 5.72RR29 pKa = 11.84PRR31 pKa = 11.84IRR33 pKa = 11.84LRR35 pKa = 11.84QHH37 pKa = 5.73HH38 pKa = 5.91TTTHH42 pKa = 4.96TTHH45 pKa = 6.13HH46 pKa = 6.6CRR48 pKa = 11.84RR49 pKa = 11.84EE50 pKa = 4.05NHH52 pKa = 4.16THH54 pKa = 6.66KK55 pKa = 10.61ATMTHH60 pKa = 6.79TIGIVAHH67 pKa = 5.88TKK69 pKa = 10.1RR70 pKa = 11.84AEE72 pKa = 3.89QAHH75 pKa = 6.23RR76 pKa = 11.84LMEE79 pKa = 4.5TVGAAYY85 pKa = 9.34MSIDD89 pKa = 3.55NGALGCEE96 pKa = 4.04ANHH99 pKa = 5.83RR100 pKa = 11.84KK101 pKa = 8.63VWQHH105 pKa = 5.26LTRR108 pKa = 11.84HH109 pKa = 4.75NTDD112 pKa = 2.58WLVVLEE118 pKa = 5.42DD119 pKa = 5.08DD120 pKa = 6.82AIPCDD125 pKa = 3.67NFRR128 pKa = 11.84DD129 pKa = 3.89QLDD132 pKa = 3.5AALAVAPSPVVSLYY146 pKa = 10.76LGRR149 pKa = 11.84EE150 pKa = 3.91RR151 pKa = 11.84PRR153 pKa = 11.84EE154 pKa = 3.87YY155 pKa = 10.07QQRR158 pKa = 11.84IAKK161 pKa = 9.98AADD164 pKa = 3.74TTAHH168 pKa = 7.01WLTCRR173 pKa = 11.84RR174 pKa = 11.84LLHH177 pKa = 6.2AVGIAIHH184 pKa = 6.94ADD186 pKa = 4.01LVPHH190 pKa = 6.61MLNNLPNGKK199 pKa = 9.05PIDD202 pKa = 3.82EE203 pKa = 5.84AISAWARR210 pKa = 11.84HH211 pKa = 4.88QSHH214 pKa = 7.09TIAYY218 pKa = 6.61TWPSLIDD225 pKa = 3.88HH226 pKa = 7.19ADD228 pKa = 3.84EE229 pKa = 4.5TPMIATRR236 pKa = 11.84NDD238 pKa = 3.26NQPRR242 pKa = 11.84TPGRR246 pKa = 11.84VAWQHH251 pKa = 4.69GTRR254 pKa = 11.84DD255 pKa = 2.96TWTTDD260 pKa = 3.15TQPII264 pKa = 3.83

Molecular weight: 30.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

16213

48

1175

186.4

20.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.689 ± 0.473

1.135 ± 0.151

6.735 ± 0.188

5.637 ± 0.324

2.936 ± 0.217

8.962 ± 0.568

2.362 ± 0.217

4.281 ± 0.189

3.121 ± 0.18

7.34 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.135

3.479 ± 0.182

5.952 ± 0.236

3.436 ± 0.187

6.908 ± 0.453

5.964 ± 0.251

6.717 ± 0.293

7.186 ± 0.308

2.368 ± 0.167

2.504 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski