Gyrovirus GyV8
Average proteome isoelectric point is 8.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G4ANJ1|A0A0G4ANJ1_9VIRU CA1 OS=Gyrovirus GyV8 OX=1670973 PE=3 SV=1
MM1 pKa = 6.64 QHH3 pKa = 5.73 VARR6 pKa = 11.84 SDD8 pKa = 3.18 VSMEE12 pKa = 4.27 YY13 pKa = 9.9 ILEE16 pKa = 3.92 PTRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 AEE24 pKa = 3.74 QNKK27 pKa = 9.68 QIAKK31 pKa = 9.29 WYY33 pKa = 8.59 RR34 pKa = 11.84 EE35 pKa = 3.86 CSRR38 pKa = 11.84 LHH40 pKa = 6.96 RR41 pKa = 11.84 NYY43 pKa = 8.56 CTCSSWQSHH52 pKa = 5.46 FRR54 pKa = 11.84 VQKK57 pKa = 9.58 PEE59 pKa = 3.81 KK60 pKa = 10.2 KK61 pKa = 9.98 NVSTQTQRR69 pKa = 11.84 TDD71 pKa = 3.07 PSVEE75 pKa = 3.29 IGYY78 pKa = 9.67 IPTGHH83 pKa = 7.58 PLFKK87 pKa = 10.7 SADD90 pKa = 3.81 RR91 pKa = 11.84 ARR93 pKa = 11.84 SKK95 pKa = 11.03 GLRR98 pKa = 11.84 SYY100 pKa = 11.15 SIDD103 pKa = 3.54 PFLSPPFTQAAEE115 pKa = 3.95 GRR117 pKa = 11.84 TGKK120 pKa = 10.25 RR121 pKa = 11.84 KK122 pKa = 9.61 RR123 pKa = 11.84 ARR125 pKa = 11.84 KK126 pKa = 8.53 QDD128 pKa = 3.7 LYY130 pKa = 11.24 CRR132 pKa = 11.84 EE133 pKa = 4.16 EE134 pKa = 5.09 LSDD137 pKa = 4.61 DD138 pKa = 5.16 DD139 pKa = 5.71 YY140 pKa = 11.81 NTTPKK145 pKa = 10.21 QLRR148 pKa = 11.84 AWLGDD153 pKa = 3.58 LGEE156 pKa = 4.74 DD157 pKa = 3.41 TTTSEE162 pKa = 4.43 EE163 pKa = 4.35 ADD165 pKa = 3.61 GTDD168 pKa = 3.27 TDD170 pKa = 4.21 GTQDD174 pKa = 3.34 TEE176 pKa = 4.93 GDD178 pKa = 3.68 MEE180 pKa = 6.45 DD181 pKa = 4.51 DD182 pKa = 3.9 FGAAITEE189 pKa = 4.45 GNINADD195 pKa = 3.85 PLIDD199 pKa = 3.69 MLSGSFSTQNLASTPFDD216 pKa = 3.64 YY217 pKa = 9.27 QTPTTDD223 pKa = 2.57 KK224 pKa = 10.56 TSFFKK229 pKa = 10.87 ASYY232 pKa = 10.16
Molecular weight: 26.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.457
IPC2_protein 5.525
IPC_protein 5.486
Toseland 5.626
ProMoST 5.715
Dawson 5.601
Bjellqvist 5.664
Wikipedia 5.537
Rodwell 5.537
Grimsley 5.652
Solomon 5.601
Lehninger 5.575
Nozaki 5.779
DTASelect 5.97
Thurlkill 5.779
EMBOSS 5.753
Sillero 5.868
Patrickios 4.266
IPC_peptide 5.601
IPC2_peptide 5.855
IPC2.peptide.svr19 5.923
Protein with the highest isoelectric point:
>tr|A0A0G4ANJ1|A0A0G4ANJ1_9VIRU CA1 OS=Gyrovirus GyV8 OX=1670973 PE=3 SV=1
MM1 pKa = 7.5 ARR3 pKa = 11.84 RR4 pKa = 11.84 PRR6 pKa = 11.84 GRR8 pKa = 11.84 YY9 pKa = 5.64 YY10 pKa = 10.43 HH11 pKa = 6.14 FRR13 pKa = 11.84 RR14 pKa = 11.84 GRR16 pKa = 11.84 WHH18 pKa = 5.91 RR19 pKa = 11.84 HH20 pKa = 3.47 RR21 pKa = 11.84 RR22 pKa = 11.84 HH23 pKa = 5.13 PRR25 pKa = 11.84 HH26 pKa = 5.42 RR27 pKa = 11.84 RR28 pKa = 11.84 GYY30 pKa = 7.97 GRR32 pKa = 11.84 RR33 pKa = 11.84 FRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 HH38 pKa = 4.42 YY39 pKa = 10.01 RR40 pKa = 11.84 GKK42 pKa = 9.93 HH43 pKa = 3.97 KK44 pKa = 10.32 RR45 pKa = 11.84 RR46 pKa = 11.84 SFNRR50 pKa = 11.84 YY51 pKa = 6.29 VKK53 pKa = 10.53 RR54 pKa = 11.84 LFFNPKK60 pKa = 8.48 PGFYY64 pKa = 9.36 TVRR67 pKa = 11.84 LPNPYY72 pKa = 9.59 NRR74 pKa = 11.84 QNIFFQGLILVPLGVDD90 pKa = 2.97 NLSAVSYY97 pKa = 10.25 KK98 pKa = 10.71 SEE100 pKa = 4.03 FCRR103 pKa = 11.84 VASITLSLRR112 pKa = 11.84 NLLLAVFPLDD122 pKa = 4.74 AISKK126 pKa = 9.84 AGGPIPDD133 pKa = 5.29 IITAYY138 pKa = 8.86 HH139 pKa = 6.49 TATEE143 pKa = 4.31 HH144 pKa = 6.09 QSQNLPPWTNMPQKK158 pKa = 10.3 GNASATPFDD167 pKa = 3.2 WWRR170 pKa = 11.84 WSLIMMRR177 pKa = 11.84 PEE179 pKa = 4.31 DD180 pKa = 3.84 NVRR183 pKa = 11.84 RR184 pKa = 11.84 AQFPYY189 pKa = 9.81 FPEE192 pKa = 4.09 RR193 pKa = 11.84 VEE195 pKa = 5.68 DD196 pKa = 3.8 LLQILGGWHH205 pKa = 7.47 LFRR208 pKa = 11.84 HH209 pKa = 6.37 IKK211 pKa = 6.81 TTMVVMATRR220 pKa = 11.84 GMSGFDD226 pKa = 3.24 PVASLFVQDD235 pKa = 4.92 SYY237 pKa = 11.38 WLSRR241 pKa = 11.84 TGNPSIINEE250 pKa = 4.07 NGNVKK255 pKa = 10.01 LQAPMPGGGVKK266 pKa = 10.36 GRR268 pKa = 11.84 IEE270 pKa = 4.21 TSTITGPPCDD280 pKa = 3.84 EE281 pKa = 4.15 YY282 pKa = 10.69 MFPSFPPQWSDD293 pKa = 2.51 KK294 pKa = 8.14 TQPTQSFKK302 pKa = 10.88 QDD304 pKa = 2.77 NAFYY308 pKa = 10.93 CLACYY313 pKa = 9.84 PSFLTLSALGAPWAFPPTEE332 pKa = 4.17 KK333 pKa = 10.53 SVSRR337 pKa = 11.84 SSFNHH342 pKa = 6.18 HH343 pKa = 6.38 SVTGNNDD350 pKa = 3.2 PQGRR354 pKa = 11.84 RR355 pKa = 11.84 WMTFLPKK362 pKa = 8.92 PTALQVPNPTGHH374 pKa = 6.93 GEE376 pKa = 4.34 TPTSSPGPTGPPPTPTEE393 pKa = 3.65 IDD395 pKa = 3.48 TPIGVLFLAQTSWGDD410 pKa = 4.01 FINHH414 pKa = 4.61 TWGTKK419 pKa = 9.18 PYY421 pKa = 10.64 NFGTTSDD428 pKa = 3.96 EE429 pKa = 4.56 SPNFPPFSRR438 pKa = 11.84 DD439 pKa = 3.08 TQNRR443 pKa = 11.84 PWAVIKK449 pKa = 10.76 LKK451 pKa = 11.03 SKK453 pKa = 8.85 WQLGNKK459 pKa = 7.5 QRR461 pKa = 11.84 PYY463 pKa = 10.35 WYY465 pKa = 7.78 DD466 pKa = 3.61 TNWWKK471 pKa = 10.48 QSRR474 pKa = 11.84 TQFGG478 pKa = 3.14
Molecular weight: 55.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.838
IPC_protein 10.672
Toseland 10.613
ProMoST 10.365
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.979
Rodwell 10.95
Grimsley 10.818
Solomon 10.847
Lehninger 10.804
Nozaki 10.599
DTASelect 10.482
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.672
Patrickios 10.613
IPC_peptide 10.847
IPC2_peptide 9.472
IPC2.peptide.svr19 8.468
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
813
103
478
271.0
31.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.289 ± 0.59
1.107 ± 0.26
5.535 ± 1.717
3.69 ± 1.154
4.92 ± 1.119
6.396 ± 0.759
2.829 ± 0.412
4.182 ± 0.603
4.428 ± 0.434
6.888 ± 0.795
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.845 ± 0.21
4.92 ± 0.709
8.118 ± 1.918
5.289 ± 0.779
9.102 ± 0.442
7.503 ± 0.573
8.241 ± 0.81
3.321 ± 0.711
2.706 ± 0.725
3.69 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here