Fusarium poae fusarivirus 1
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA67|A0A1B4ZA67_9VIRU RNA-directed RNA polymerase OS=Fusarium poae fusarivirus 1 OX=1849537 PE=4 SV=1
MM1 pKa = 7.39 SGKK4 pKa = 10.62 GSDD7 pKa = 3.3 RR8 pKa = 11.84 SEE10 pKa = 4.58 DD11 pKa = 3.66 FQSVAEE17 pKa = 4.24 SQMTQHH23 pKa = 6.95 RR24 pKa = 11.84 SLSISEE30 pKa = 4.63 GIKK33 pKa = 10.43 KK34 pKa = 10.03 VIQEE38 pKa = 4.04 FSDD41 pKa = 3.33 RR42 pKa = 11.84 ATFKK46 pKa = 11.15 GNAVQVIDD54 pKa = 5.29 AEE56 pKa = 4.53 SLDD59 pKa = 4.05 LLAKK63 pKa = 10.02 RR64 pKa = 11.84 VMEE67 pKa = 4.27 VLSTDD72 pKa = 3.52 ADD74 pKa = 3.95 TTVPKK79 pKa = 9.9 STLAEE84 pKa = 4.27 LSAQLAKK91 pKa = 10.21 IRR93 pKa = 11.84 LEE95 pKa = 3.8 ASTFKK100 pKa = 10.99 DD101 pKa = 3.09 RR102 pKa = 11.84 AEE104 pKa = 3.91 KK105 pKa = 11.13 AEE107 pKa = 4.08 FAKK110 pKa = 10.84 SEE112 pKa = 4.16 ADD114 pKa = 3.25 TTLAALQQEE123 pKa = 4.28 LAKK126 pKa = 10.79 FKK128 pKa = 11.35 VEE130 pKa = 3.93 ANKK133 pKa = 9.98 QVSQLKK139 pKa = 9.87 SDD141 pKa = 3.37 KK142 pKa = 10.9 AGYY145 pKa = 10.65 EE146 pKa = 3.79 MDD148 pKa = 3.89 KK149 pKa = 11.05 VQAQAALKK157 pKa = 9.76 EE158 pKa = 4.34 LRR160 pKa = 11.84 AHH162 pKa = 6.47 MKK164 pKa = 10.3 AFPDD168 pKa = 3.51 GTTTVEE174 pKa = 4.05 KK175 pKa = 11.09 EE176 pKa = 4.14 EE177 pKa = 4.15 VAEE180 pKa = 3.94 QHH182 pKa = 6.47 EE183 pKa = 4.52 NLSKK187 pKa = 10.83 QVTSLNNAAQDD198 pKa = 3.29 ANAQIKK204 pKa = 10.15 RR205 pKa = 11.84 LRR207 pKa = 11.84 QKK209 pKa = 8.92 EE210 pKa = 4.25 TLLEE214 pKa = 3.94 KK215 pKa = 10.65 HH216 pKa = 6.26 ILSLSNEE223 pKa = 3.83 INIARR228 pKa = 11.84 ASIPPARR235 pKa = 11.84 APGAKK240 pKa = 9.75 SFSEE244 pKa = 4.33 VAKK247 pKa = 10.58 AAGSANVDD255 pKa = 3.41 LTKK258 pKa = 10.73 AAFSLLDD265 pKa = 3.42 KK266 pKa = 11.0 RR267 pKa = 11.84 SLEE270 pKa = 4.22 KK271 pKa = 10.5 LRR273 pKa = 11.84 KK274 pKa = 9.56 AYY276 pKa = 10.15 EE277 pKa = 4.11 STDD280 pKa = 3.37 GVKK283 pKa = 10.7 DD284 pKa = 3.3 KK285 pKa = 11.38 LFWLKK290 pKa = 10.99 AGLQNAKK297 pKa = 10.39 NSLFKK302 pKa = 10.63 GYY304 pKa = 10.87 YY305 pKa = 8.41 NVAEE309 pKa = 4.45 ALHH312 pKa = 6.42 KK313 pKa = 10.67 EE314 pKa = 4.47 CLLMDD319 pKa = 3.72 YY320 pKa = 10.26 PSRR323 pKa = 11.84 SKK325 pKa = 9.64 TLSHH329 pKa = 6.79 VVDD332 pKa = 3.51 NMLAEE337 pKa = 4.5 MEE339 pKa = 4.39 TEE341 pKa = 3.76 LDD343 pKa = 3.48 GDD345 pKa = 4.0 EE346 pKa = 4.62 KK347 pKa = 10.53 SVRR350 pKa = 11.84 MKK352 pKa = 10.61 FYY354 pKa = 10.54 TKK356 pKa = 10.2 TSPDD360 pKa = 3.24 QMRR363 pKa = 11.84 LKK365 pKa = 10.17 PALAKK370 pKa = 9.79 TVGATYY376 pKa = 10.81 LSQAYY381 pKa = 10.13 LKK383 pKa = 10.88 GWDD386 pKa = 4.23 LDD388 pKa = 4.04 DD389 pKa = 6.1 LVDD392 pKa = 5.69 LEE394 pKa = 4.74 KK395 pKa = 10.74 QLHH398 pKa = 5.62 TKK400 pKa = 9.31 TFTKK404 pKa = 9.66 TQDD407 pKa = 3.29 PPVKK411 pKa = 10.49 VKK413 pKa = 10.49 EE414 pKa = 4.53 ADD416 pKa = 3.59 NSWAEE421 pKa = 4.12 IFPEE425 pKa = 4.37 IDD427 pKa = 3.25 PSGVPLPPPTPDD439 pKa = 4.17 EE440 pKa = 4.81 NPQSWWRR447 pKa = 11.84 RR448 pKa = 11.84 LLFGTSALFASLGFIGKK465 pKa = 8.28 SLWAPVGKK473 pKa = 9.37 FFSMINVFKK482 pKa = 11.08
Molecular weight: 53.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.818
IPC2_protein 7.044
IPC_protein 7.293
Toseland 7.805
ProMoST 7.936
Dawson 8.083
Bjellqvist 7.688
Wikipedia 8.068
Rodwell 8.112
Grimsley 7.717
Solomon 8.331
Lehninger 8.331
Nozaki 7.98
DTASelect 7.966
Thurlkill 8.097
EMBOSS 8.361
Sillero 8.214
Patrickios 5.08
IPC_peptide 8.331
IPC2_peptide 6.927
IPC2.peptide.svr19 7.336
Protein with the highest isoelectric point:
>tr|A0A1B4ZA67|A0A1B4ZA67_9VIRU RNA-directed RNA polymerase OS=Fusarium poae fusarivirus 1 OX=1849537 PE=4 SV=1
MM1 pKa = 7.42 CFCVLFMGLLALVSSVPCIIFWVVCPFLSPWVALWISGLLPSFISVLMLGGVFTARR57 pKa = 11.84 RR58 pKa = 11.84 FVYY61 pKa = 10.43 RR62 pKa = 11.84 EE63 pKa = 3.92 AEE65 pKa = 4.72 LIRR68 pKa = 11.84 QFGPEE73 pKa = 3.37 HH74 pKa = 6.41 ALWVDD79 pKa = 3.26 SGSWFSFRR87 pKa = 11.84 RR88 pKa = 11.84 HH89 pKa = 5.15 ASIVVAGIDD98 pKa = 3.89 PLIGCPSEE106 pKa = 5.83 DD107 pKa = 2.89 IVLSSLRR114 pKa = 11.84 RR115 pKa = 11.84 INGTHH120 pKa = 7.01 KK121 pKa = 10.84 LSGWYY126 pKa = 9.58 EE127 pKa = 3.88 NSSLLWSSCILYY139 pKa = 10.7 AKK141 pKa = 9.6 TLMNFSPTLWYY152 pKa = 9.59 TFGAFVFSRR161 pKa = 11.84 AFRR164 pKa = 11.84 PVRR167 pKa = 11.84 SVVEE171 pKa = 3.55 NFYY174 pKa = 11.02 RR175 pKa = 11.84 VLKK178 pKa = 8.81 WGVYY182 pKa = 9.38 VMLLLATVSTSSLVSFVMISSAILSSLWHH211 pKa = 6.54 ANFGGWYY218 pKa = 9.95 YY219 pKa = 11.32 RR220 pKa = 11.84 MKK222 pKa = 11.05 LEE224 pKa = 3.86 LTILVVEE231 pKa = 4.84 AVNVWLEE238 pKa = 3.93 FNFTGVKK245 pKa = 8.96 WFSKK249 pKa = 10.88 DD250 pKa = 2.76 GFTVRR255 pKa = 11.84 KK256 pKa = 8.59 GVKK259 pKa = 9.53 GKK261 pKa = 10.73 AYY263 pKa = 10.56 LADD266 pKa = 3.88 SLSKK270 pKa = 10.83 LGIVIADD277 pKa = 4.35 LGLPNYY283 pKa = 9.53 ILSKK287 pKa = 10.5 QPQSYY292 pKa = 10.26 SADD295 pKa = 4.12 DD296 pKa = 3.41 IKK298 pKa = 11.49 SSLEE302 pKa = 3.7 VMRR305 pKa = 11.84 DD306 pKa = 3.38 LGWPINVEE314 pKa = 3.73 LADD317 pKa = 3.83 PSRR320 pKa = 11.84 FSSSSAYY327 pKa = 10.43 ADD329 pKa = 3.23 WLVSGSNWQQGLHH342 pKa = 4.7 QRR344 pKa = 11.84 QMYY347 pKa = 9.47 ADD349 pKa = 5.16 KK350 pKa = 10.78 ILDD353 pKa = 3.81 PLRR356 pKa = 11.84 VKK358 pKa = 10.53 AVEE361 pKa = 3.94 FRR363 pKa = 11.84 RR364 pKa = 11.84 TEE366 pKa = 3.83 EE367 pKa = 4.03 YY368 pKa = 10.0 MSLEE372 pKa = 4.07 NEE374 pKa = 4.25 LEE376 pKa = 4.23 SIARR380 pKa = 11.84 YY381 pKa = 8.78 FKK383 pKa = 10.65 SPRR386 pKa = 11.84 YY387 pKa = 9.38 DD388 pKa = 4.14 FPDD391 pKa = 4.0 LPLDD395 pKa = 4.39 DD396 pKa = 4.03 VWFLIGDD403 pKa = 3.82 IFKK406 pKa = 10.57 FSKK409 pKa = 10.33 LAPFNHH415 pKa = 7.7 IIRR418 pKa = 11.84 LWEE421 pKa = 3.75 KK422 pKa = 10.41 KK423 pKa = 9.29 YY424 pKa = 11.03 ALGSFMVDD432 pKa = 3.1 PSNPRR437 pKa = 11.84 KK438 pKa = 10.01 KK439 pKa = 9.04 YY440 pKa = 10.35 SRR442 pKa = 11.84 WKK444 pKa = 9.54 FISTIGYY451 pKa = 10.03 RR452 pKa = 11.84 SFKK455 pKa = 10.14 EE456 pKa = 3.61 LWRR459 pKa = 11.84 KK460 pKa = 6.16 TFEE463 pKa = 4.19 VASLITPVAHH473 pKa = 6.15 VSVKK477 pKa = 10.91 GEE479 pKa = 3.82 ALPPKK484 pKa = 10.18 KK485 pKa = 10.23 FLLDD489 pKa = 3.37 KK490 pKa = 10.77 VRR492 pKa = 11.84 TVVGSPLGQYY502 pKa = 9.95 ILSTVFNFYY511 pKa = 10.12 PNHH514 pKa = 5.85 NFRR517 pKa = 11.84 FEE519 pKa = 4.03 STPIKK524 pKa = 10.71 VGMPLNGYY532 pKa = 7.73 WFNKK536 pKa = 8.31 VYY538 pKa = 10.45 NRR540 pKa = 11.84 HH541 pKa = 5.55 SRR543 pKa = 11.84 CQVHH547 pKa = 6.0 FAGDD551 pKa = 3.74 FSDD554 pKa = 4.94 FDD556 pKa = 3.79 STLSGKK562 pKa = 8.94 VRR564 pKa = 11.84 EE565 pKa = 4.37 IIKK568 pKa = 10.28 AVRR571 pKa = 11.84 KK572 pKa = 10.02 RR573 pKa = 11.84 GFSSHH578 pKa = 6.65 KK579 pKa = 10.61 DD580 pKa = 2.86 VDD582 pKa = 4.85 RR583 pKa = 11.84 ICKK586 pKa = 10.36 LIDD589 pKa = 3.65 LNYY592 pKa = 10.8 DD593 pKa = 3.27 QVNNQLLNTTSTGDD607 pKa = 3.07 IFGKK611 pKa = 9.16 GTGLTTGHH619 pKa = 6.66 SSTTMDD625 pKa = 3.4 NSIGSIVLYY634 pKa = 10.61 LMAWRR639 pKa = 11.84 EE640 pKa = 4.08 LTGMNAQAFKK650 pKa = 10.65 HH651 pKa = 5.18 YY652 pKa = 11.01 VEE654 pKa = 5.65 LDD656 pKa = 3.46 VFGDD660 pKa = 3.45 DD661 pKa = 6.34 HH662 pKa = 7.61 IISWLATSPVAWNFPNIQQVMARR685 pKa = 11.84 WGLEE689 pKa = 3.58 LRR691 pKa = 11.84 EE692 pKa = 4.08 EE693 pKa = 4.26 ASGKK697 pKa = 10.53 LEE699 pKa = 4.23 ALPFLSKK706 pKa = 10.29 FVRR709 pKa = 11.84 RR710 pKa = 11.84 PNPTDD715 pKa = 3.32 VAEE718 pKa = 4.35 MKK720 pKa = 10.73 AAGVWSHH727 pKa = 6.08 GSWIVYY733 pKa = 9.28 HH734 pKa = 7.27 DD735 pKa = 3.79 KK736 pKa = 11.29 ARR738 pKa = 11.84 LLGKK742 pKa = 10.0 LVSKK746 pKa = 10.28 VKK748 pKa = 9.99 TMAPEE753 pKa = 3.79 YY754 pKa = 9.85 RR755 pKa = 11.84 LKK757 pKa = 10.84 RR758 pKa = 11.84 LLSYY762 pKa = 11.12 LSLTAHH768 pKa = 6.68 HH769 pKa = 7.0 KK770 pKa = 10.54 DD771 pKa = 3.69 IYY773 pKa = 10.76 DD774 pKa = 3.33 QLRR777 pKa = 11.84 NIIARR782 pKa = 11.84 TNSFAGILRR791 pKa = 11.84 SHH793 pKa = 7.0 KK794 pKa = 10.32 FSIPSYY800 pKa = 10.47 EE801 pKa = 4.46 KK802 pKa = 10.84 VLQDD806 pKa = 3.66 WYY808 pKa = 9.56 TSNAIEE814 pKa = 4.21 PHH816 pKa = 6.0 QEE818 pKa = 3.43 MDD820 pKa = 4.23 EE821 pKa = 4.26 IDD823 pKa = 4.05 PSLVDD828 pKa = 3.76 SGLVSYY834 pKa = 10.86 GSVSAFDD841 pKa = 3.61 SVLGILAQVPDD852 pKa = 4.91 IINPVLFNFGYY863 pKa = 10.55 LRR865 pKa = 11.84 AFQARR870 pKa = 11.84 GAHH873 pKa = 5.92 LFDD876 pKa = 4.02 WPFQLLEE883 pKa = 4.03 HH884 pKa = 6.65 TNNVWGPGEE893 pKa = 4.81 LSFLMQRR900 pKa = 11.84 SPYY903 pKa = 10.57 SFLDD907 pKa = 3.2 PTIRR911 pKa = 11.84 SGSEE915 pKa = 3.46 RR916 pKa = 11.84 SNLTSLIVRR925 pKa = 11.84 NFIFLQLKK933 pKa = 9.51 NAFKK937 pKa = 10.6 IPSVLVRR944 pKa = 11.84 LGSAHH949 pKa = 7.21 RR950 pKa = 11.84 KK951 pKa = 7.51 FAQVQFWLNGKK962 pKa = 9.64 VMDD965 pKa = 4.69 DD966 pKa = 3.51 VRR968 pKa = 11.84 SGEE971 pKa = 4.02 LPIWQMTLIAMLAYY985 pKa = 10.42 VSLPDD990 pKa = 3.54 IFPWLAEE997 pKa = 3.82 VSVPNIPLVIEE1008 pKa = 4.33 KK1009 pKa = 10.83 GFLSLSNFFWSSLPPNYY1026 pKa = 10.47 ADD1028 pKa = 4.78 VITQLRR1034 pKa = 11.84 HH1035 pKa = 5.3 LAPVSGSKK1043 pKa = 10.7 LSVAAPTGSGKK1054 pKa = 8.52 STALIKK1060 pKa = 10.59 AVADD1064 pKa = 3.68 HH1065 pKa = 6.8 LPLNFSRR1072 pKa = 11.84 VVVIEE1077 pKa = 3.82 PRR1079 pKa = 11.84 SAIVRR1084 pKa = 11.84 GLVPYY1089 pKa = 7.31 TQRR1092 pKa = 11.84 QLALDD1097 pKa = 3.69 SSGATSGMTLDD1108 pKa = 3.79 TKK1110 pKa = 11.25 AKK1112 pKa = 8.18 VWYY1115 pKa = 8.69 VTAQEE1120 pKa = 4.69 ALMHH1124 pKa = 5.69 TKK1126 pKa = 8.41 WWSSDD1131 pKa = 3.15 TLFVMDD1137 pKa = 4.66 EE1138 pKa = 4.03 CHH1140 pKa = 6.9 IDD1142 pKa = 3.25 EE1143 pKa = 4.51 TAYY1146 pKa = 11.34 LLLKK1150 pKa = 10.51 QLFAKK1155 pKa = 10.51 RR1156 pKa = 11.84 EE1157 pKa = 3.95 EE1158 pKa = 4.17 LVVYY1162 pKa = 9.8 LSATPPQDD1170 pKa = 3.98 IIDD1173 pKa = 4.37 ASLHH1177 pKa = 6.16 LPLNTARR1184 pKa = 11.84 VWNLAVSHH1192 pKa = 5.54 MQRR1195 pKa = 11.84 KK1196 pKa = 8.84 GLLSYY1201 pKa = 10.89 KK1202 pKa = 9.39 EE1203 pKa = 3.73 AWLDD1207 pKa = 3.99 FKK1209 pKa = 11.26 HH1210 pKa = 6.21 QALLLLSSLPKK1221 pKa = 10.27 ASRR1224 pKa = 11.84 SLIFVNTVKK1233 pKa = 10.39 EE1234 pKa = 4.2 ANEE1237 pKa = 3.76 LATAIGSRR1245 pKa = 11.84 AVVLSSSSDD1254 pKa = 2.85 HH1255 pKa = 6.9 SLIHH1259 pKa = 6.62 DD1260 pKa = 4.06 ASVIVSTSVADD1271 pKa = 3.74 VGVTIPDD1278 pKa = 3.25 VDD1280 pKa = 4.02 TVLTSDD1286 pKa = 2.74 IGFTVVEE1293 pKa = 4.72 DD1294 pKa = 3.89 FDD1296 pKa = 4.04 NARR1299 pKa = 11.84 GVYY1302 pKa = 9.4 YY1303 pKa = 10.42 RR1304 pKa = 11.84 LSGSQLRR1311 pKa = 11.84 QRR1313 pKa = 11.84 VGRR1316 pKa = 11.84 TGRR1319 pKa = 11.84 TNHH1322 pKa = 5.82 GQGKK1326 pKa = 8.83 VLLYY1330 pKa = 10.24 PDD1332 pKa = 4.47 VPTHH1336 pKa = 6.74 DD1337 pKa = 4.08 VSASLQTPSAVLDD1350 pKa = 3.97 LLKK1353 pKa = 10.11 TGVPVRR1359 pKa = 11.84 VMAEE1363 pKa = 4.0 HH1364 pKa = 7.18 LSGPLEE1370 pKa = 4.18 KK1371 pKa = 10.94 LLGVDD1376 pKa = 3.41 NAEE1379 pKa = 4.35 GPAARR1384 pKa = 11.84 DD1385 pKa = 3.41 MVDD1388 pKa = 4.11 FAFTQLDD1395 pKa = 3.84 RR1396 pKa = 11.84 YY1397 pKa = 10.42 SEE1399 pKa = 4.13 NFSLLLQEE1407 pKa = 4.81 RR1408 pKa = 11.84 AASSEE1413 pKa = 4.11 LPTATGEE1420 pKa = 4.26 APAHH1424 pKa = 6.43 IDD1426 pKa = 3.36 NARR1429 pKa = 11.84 MGLVRR1434 pKa = 11.84 ISSMLPSSQIVSSMVDD1450 pKa = 2.91 IVSSLAQAAFSTGEE1464 pKa = 3.85 VKK1466 pKa = 10.38 DD1467 pKa = 4.23 YY1468 pKa = 10.89 YY1469 pKa = 9.75 MEE1471 pKa = 4.42 RR1472 pKa = 11.84 AEE1474 pKa = 4.33 VATNKK1479 pKa = 10.0 IGFGVKK1485 pKa = 9.9 GLRR1488 pKa = 11.84 PLPDD1492 pKa = 4.2 PSLGEE1497 pKa = 3.96 MGFDD1501 pKa = 3.44
Molecular weight: 168.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.951
IPC2_protein 8.112
IPC_protein 7.966
Toseland 8.375
ProMoST 8.492
Dawson 8.858
Bjellqvist 8.814
Wikipedia 8.975
Rodwell 8.96
Grimsley 8.726
Solomon 8.902
Lehninger 8.873
Nozaki 8.858
DTASelect 8.712
Thurlkill 8.785
EMBOSS 8.99
Sillero 8.975
Patrickios 4.52
IPC_peptide 8.887
IPC2_peptide 7.527
IPC2.peptide.svr19 7.633
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1983
482
1501
991.5
110.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.119 ± 1.547
0.504 ± 0.139
5.396 ± 0.389
4.992 ± 1.261
5.144 ± 0.467
5.396 ± 0.683
2.168 ± 0.337
4.639 ± 0.815
6.152 ± 1.984
11.346 ± 0.36
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.269 ± 0.006
3.48 ± 0.075
4.589 ± 0.304
3.227 ± 0.726
4.892 ± 0.544
9.934 ± 0.574
4.791 ± 0.479
7.867 ± 1.064
2.269 ± 0.481
2.824 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here