Pseudomonas phage vB_PsyM_KIL2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Flaumdravirus; Pseudomonas virus KIL4; Pseudomonas phage vB_PsyM_KIL1

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 161 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142IDY5|A0A142IDY5_9CAUD Putative HNH endonuclease OS=Pseudomonas phage vB_PsyM_KIL2 OX=1777066 GN=vB_PsyM_KIL2_0029 PE=4 SV=1
MM1 pKa = 7.39SVTIVSVYY9 pKa = 10.83LKK11 pKa = 9.74TEE13 pKa = 3.87SGDD16 pKa = 3.75GYY18 pKa = 10.97LFTYY22 pKa = 10.64DD23 pKa = 2.99RR24 pKa = 11.84VEE26 pKa = 4.17SVDD29 pKa = 3.5EE30 pKa = 4.22FVEE33 pKa = 4.84LVEE36 pKa = 5.55KK37 pKa = 11.05DD38 pKa = 3.59LGDD41 pKa = 3.63EE42 pKa = 3.92LAYY45 pKa = 10.71VYY47 pKa = 10.8SIDD50 pKa = 3.9LNAMYY55 pKa = 9.67TRR57 pKa = 11.84TEE59 pKa = 3.97TYY61 pKa = 9.51EE62 pKa = 4.12YY63 pKa = 10.93EE64 pKa = 4.36DD65 pKa = 4.05ALQKK69 pKa = 10.54RR70 pKa = 11.84INEE73 pKa = 4.22MADD76 pKa = 3.34DD77 pKa = 4.0EE78 pKa = 4.56

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142IEB1|A0A142IEB1_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PsyM_KIL2 OX=1777066 GN=vB_PsyM_KIL2_0166 PE=4 SV=1
MM1 pKa = 7.95AITITSLTHH10 pKa = 5.31PTMIAKK16 pKa = 9.14IRR18 pKa = 11.84KK19 pKa = 7.66GMYY22 pKa = 8.8YY23 pKa = 9.22AHH25 pKa = 7.65KK26 pKa = 10.26GQGHH30 pKa = 5.71MMNGHH35 pKa = 5.48GTRR38 pKa = 11.84VYY40 pKa = 10.17IEE42 pKa = 3.84NAKK45 pKa = 9.2GHH47 pKa = 5.45NVMRR51 pKa = 11.84LDD53 pKa = 3.59WVGGRR58 pKa = 11.84QGYY61 pKa = 8.9IVYY64 pKa = 9.4GQEE67 pKa = 3.94SRR69 pKa = 11.84DD70 pKa = 2.88ITAIVKK76 pKa = 10.03RR77 pKa = 11.84ALHH80 pKa = 6.55RR81 pKa = 11.84DD82 pKa = 3.51VQRR85 pKa = 11.84AMHH88 pKa = 6.25NVTSFEE94 pKa = 4.18RR95 pKa = 11.84CFGKK99 pKa = 9.73PAGSPRR105 pKa = 11.84LIRR108 pKa = 11.84IAALAALSAGLTGCQSSGVVTAVCSLVGGLIVV140 pKa = 4.08

Molecular weight: 15.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

161

0

161

25706

27

803

159.7

17.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.66 ± 0.291

1.478 ± 0.127

5.925 ± 0.172

6.734 ± 0.263

3.964 ± 0.135

7.232 ± 0.28

1.914 ± 0.122

5.621 ± 0.166

6.675 ± 0.263

8.119 ± 0.189

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.778 ± 0.12

4.793 ± 0.195

3.486 ± 0.151

3.859 ± 0.118

4.898 ± 0.204

6.594 ± 0.249

6.065 ± 0.212

6.882 ± 0.175

1.696 ± 0.087

3.63 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski