Amino acid dipepetide frequency for Pseudomonas phage vB_PsyM_KIL2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.08AlaAla: 7.08 ± 0.747
1.05AlaCys: 1.05 ± 0.239
4.902AlaAsp: 4.902 ± 0.48
4.629AlaGlu: 4.629 ± 0.411
3.112AlaPhe: 3.112 ± 0.356
4.98AlaGly: 4.98 ± 0.418
1.401AlaHis: 1.401 ± 0.222
4.707AlaIle: 4.707 ± 0.351
4.668AlaLys: 4.668 ± 0.421
6.263AlaLeu: 6.263 ± 0.588
2.179AlaMet: 2.179 ± 0.335
3.073AlaAsn: 3.073 ± 0.356
2.179AlaPro: 2.179 ± 0.3
3.579AlaGln: 3.579 ± 0.307
3.968AlaArg: 3.968 ± 0.45
4.902AlaSer: 4.902 ± 0.448
5.174AlaThr: 5.174 ± 0.497
5.135AlaVal: 5.135 ± 0.524
1.284AlaTrp: 1.284 ± 0.227
2.334AlaTyr: 2.334 ± 0.255
0.0AlaXaa: 0.0 ± 0.0
Cys
0.895CysAla: 0.895 ± 0.185
0.311CysCys: 0.311 ± 0.109
0.778CysAsp: 0.778 ± 0.21
0.856CysGlu: 0.856 ± 0.219
0.622CysPhe: 0.622 ± 0.15
1.05CysGly: 1.05 ± 0.211
0.35CysHis: 0.35 ± 0.119
0.817CysIle: 0.817 ± 0.172
1.011CysLys: 1.011 ± 0.218
0.973CysLeu: 0.973 ± 0.201
0.467CysMet: 0.467 ± 0.129
0.661CysAsn: 0.661 ± 0.182
0.661CysPro: 0.661 ± 0.157
0.506CysGln: 0.506 ± 0.134
0.584CysArg: 0.584 ± 0.134
1.089CysSer: 1.089 ± 0.208
1.128CysThr: 1.128 ± 0.231
1.128CysVal: 1.128 ± 0.178
0.117CysTrp: 0.117 ± 0.071
0.778CysTyr: 0.778 ± 0.184
0.0CysXaa: 0.0 ± 0.0
Asp
3.968AspAla: 3.968 ± 0.393
1.089AspCys: 1.089 ± 0.178
3.346AspAsp: 3.346 ± 0.349
3.851AspGlu: 3.851 ± 0.447
2.529AspPhe: 2.529 ± 0.342
4.902AspGly: 4.902 ± 0.476
1.05AspHis: 1.05 ± 0.199
2.684AspIle: 2.684 ± 0.394
4.824AspLys: 4.824 ± 0.462
5.563AspLeu: 5.563 ± 0.415
2.023AspMet: 2.023 ± 0.262
2.568AspAsn: 2.568 ± 0.321
1.79AspPro: 1.79 ± 0.269
1.828AspGln: 1.828 ± 0.24
2.606AspArg: 2.606 ± 0.3
3.501AspSer: 3.501 ± 0.379
3.268AspThr: 3.268 ± 0.339
3.812AspVal: 3.812 ± 0.387
1.673AspTrp: 1.673 ± 0.24
2.373AspTyr: 2.373 ± 0.29
0.0AspXaa: 0.0 ± 0.0
Glu
5.758GluAla: 5.758 ± 0.539
1.323GluCys: 1.323 ± 0.262
3.968GluAsp: 3.968 ± 0.428
5.913GluGlu: 5.913 ± 0.58
3.423GluPhe: 3.423 ± 0.365
3.423GluGly: 3.423 ± 0.352
1.284GluHis: 1.284 ± 0.208
4.046GluIle: 4.046 ± 0.403
3.851GluLys: 3.851 ± 0.465
5.408GluLeu: 5.408 ± 0.532
2.373GluMet: 2.373 ± 0.27
2.49GluAsn: 2.49 ± 0.303
1.751GluPro: 1.751 ± 0.236
2.684GluGln: 2.684 ± 0.379
3.735GluArg: 3.735 ± 0.438
3.851GluSer: 3.851 ± 0.39
3.073GluThr: 3.073 ± 0.355
4.824GluVal: 4.824 ± 0.39
1.634GluTrp: 1.634 ± 0.227
2.49GluTyr: 2.49 ± 0.244
0.0GluXaa: 0.0 ± 0.0
Phe
2.334PheAla: 2.334 ± 0.34
0.7PheCys: 0.7 ± 0.168
2.645PheAsp: 2.645 ± 0.332
3.307PheGlu: 3.307 ± 0.334
1.323PhePhe: 1.323 ± 0.231
3.151PheGly: 3.151 ± 0.335
0.739PheHis: 0.739 ± 0.159
2.101PheIle: 2.101 ± 0.356
2.645PheLys: 2.645 ± 0.315
3.073PheLeu: 3.073 ± 0.35
1.089PheMet: 1.089 ± 0.208
2.023PheAsn: 2.023 ± 0.251
1.439PhePro: 1.439 ± 0.243
1.478PheGln: 1.478 ± 0.204
1.828PheArg: 1.828 ± 0.285
3.229PheSer: 3.229 ± 0.364
2.723PheThr: 2.723 ± 0.338
2.568PheVal: 2.568 ± 0.341
0.311PheTrp: 0.311 ± 0.13
0.934PheTyr: 0.934 ± 0.163
0.0PheXaa: 0.0 ± 0.0
Gly
4.007GlyAla: 4.007 ± 0.418
1.362GlyCys: 1.362 ± 0.216
3.851GlyAsp: 3.851 ± 0.393
4.085GlyGlu: 4.085 ± 0.308
3.229GlyPhe: 3.229 ± 0.332
5.446GlyGly: 5.446 ± 0.525
1.478GlyHis: 1.478 ± 0.24
3.968GlyIle: 3.968 ± 0.464
5.641GlyLys: 5.641 ± 0.52
6.808GlyLeu: 6.808 ± 0.571
2.023GlyMet: 2.023 ± 0.253
3.657GlyAsn: 3.657 ± 0.518
1.089GlyPro: 1.089 ± 0.232
1.906GlyGln: 1.906 ± 0.259
3.501GlyArg: 3.501 ± 0.385
5.174GlySer: 5.174 ± 0.475
3.89GlyThr: 3.89 ± 0.441
6.186GlyVal: 6.186 ± 0.436
1.401GlyTrp: 1.401 ± 0.221
3.618GlyTyr: 3.618 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
1.206HisAla: 1.206 ± 0.232
0.233HisCys: 0.233 ± 0.085
1.011HisAsp: 1.011 ± 0.187
1.011HisGlu: 1.011 ± 0.179
0.817HisPhe: 0.817 ± 0.169
1.362HisGly: 1.362 ± 0.202
0.506HisHis: 0.506 ± 0.186
0.934HisIle: 0.934 ± 0.188
1.206HisLys: 1.206 ± 0.227
1.517HisLeu: 1.517 ± 0.235
0.622HisMet: 0.622 ± 0.175
1.089HisAsn: 1.089 ± 0.163
0.895HisPro: 0.895 ± 0.207
0.661HisGln: 0.661 ± 0.146
1.206HisArg: 1.206 ± 0.19
1.517HisSer: 1.517 ± 0.165
1.05HisThr: 1.05 ± 0.179
1.167HisVal: 1.167 ± 0.197
0.35HisTrp: 0.35 ± 0.13
0.778HisTyr: 0.778 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
3.968IleAla: 3.968 ± 0.396
0.895IleCys: 0.895 ± 0.188
3.812IleAsp: 3.812 ± 0.341
4.591IleGlu: 4.591 ± 0.406
1.673IlePhe: 1.673 ± 0.264
3.968IleGly: 3.968 ± 0.438
1.167IleHis: 1.167 ± 0.202
2.684IleIle: 2.684 ± 0.289
3.735IleLys: 3.735 ± 0.311
4.357IleLeu: 4.357 ± 0.449
1.167IleMet: 1.167 ± 0.236
3.151IleAsn: 3.151 ± 0.408
2.334IlePro: 2.334 ± 0.272
2.256IleGln: 2.256 ± 0.293
3.19IleArg: 3.19 ± 0.282
3.968IleSer: 3.968 ± 0.451
4.007IleThr: 4.007 ± 0.396
3.268IleVal: 3.268 ± 0.432
0.7IleTrp: 0.7 ± 0.166
1.323IleTyr: 1.323 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
6.224LysAla: 6.224 ± 0.616
0.778LysCys: 0.778 ± 0.207
4.396LysAsp: 4.396 ± 0.36
4.435LysGlu: 4.435 ± 0.509
2.217LysPhe: 2.217 ± 0.279
4.279LysGly: 4.279 ± 0.406
1.284LysHis: 1.284 ± 0.222
4.046LysIle: 4.046 ± 0.37
3.423LysLys: 3.423 ± 0.326
4.513LysLeu: 4.513 ± 0.471
2.529LysMet: 2.529 ± 0.327
2.801LysAsn: 2.801 ± 0.333
2.606LysPro: 2.606 ± 0.333
2.762LysGln: 2.762 ± 0.297
3.812LysArg: 3.812 ± 0.39
4.163LysSer: 4.163 ± 0.422
3.929LysThr: 3.929 ± 0.418
5.135LysVal: 5.135 ± 0.477
0.856LysTrp: 0.856 ± 0.168
2.568LysTyr: 2.568 ± 0.332
0.0LysXaa: 0.0 ± 0.0
Leu
6.73LeuAla: 6.73 ± 0.512
0.934LeuCys: 0.934 ± 0.188
5.174LeuAsp: 5.174 ± 0.436
6.108LeuGlu: 6.108 ± 0.46
3.151LeuPhe: 3.151 ± 0.371
5.913LeuGly: 5.913 ± 0.55
1.089LeuHis: 1.089 ± 0.199
4.007LeuIle: 4.007 ± 0.43
5.758LeuLys: 5.758 ± 0.535
5.719LeuLeu: 5.719 ± 0.611
2.256LeuMet: 2.256 ± 0.262
4.163LeuAsn: 4.163 ± 0.462
3.696LeuPro: 3.696 ± 0.313
3.657LeuGln: 3.657 ± 0.372
3.735LeuArg: 3.735 ± 0.399
5.485LeuSer: 5.485 ± 0.437
4.941LeuThr: 4.941 ± 0.52
4.668LeuVal: 4.668 ± 0.546
1.362LeuTrp: 1.362 ± 0.231
2.645LeuTyr: 2.645 ± 0.329
0.0LeuXaa: 0.0 ± 0.0
Met
2.723MetAla: 2.723 ± 0.331
0.272MetCys: 0.272 ± 0.125
1.05MetAsp: 1.05 ± 0.195
1.984MetGlu: 1.984 ± 0.29
1.245MetPhe: 1.245 ± 0.24
1.556MetGly: 1.556 ± 0.217
0.7MetHis: 0.7 ± 0.169
1.79MetIle: 1.79 ± 0.235
2.179MetLys: 2.179 ± 0.32
2.023MetLeu: 2.023 ± 0.313
0.506MetMet: 0.506 ± 0.166
1.245MetAsn: 1.245 ± 0.25
0.973MetPro: 0.973 ± 0.197
1.206MetGln: 1.206 ± 0.216
1.05MetArg: 1.05 ± 0.202
2.179MetSer: 2.179 ± 0.218
1.867MetThr: 1.867 ± 0.271
1.79MetVal: 1.79 ± 0.284
0.389MetTrp: 0.389 ± 0.123
1.05MetTyr: 1.05 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
3.073AsnAla: 3.073 ± 0.391
0.661AsnCys: 0.661 ± 0.142
2.762AsnAsp: 2.762 ± 0.345
2.062AsnGlu: 2.062 ± 0.268
1.751AsnPhe: 1.751 ± 0.294
4.513AsnGly: 4.513 ± 0.341
1.167AsnHis: 1.167 ± 0.173
3.268AsnIle: 3.268 ± 0.413
3.346AsnLys: 3.346 ± 0.353
4.202AsnLeu: 4.202 ± 0.512
0.817AsnMet: 0.817 ± 0.175
2.723AsnAsn: 2.723 ± 0.418
2.334AsnPro: 2.334 ± 0.327
1.284AsnGln: 1.284 ± 0.248
2.373AsnArg: 2.373 ± 0.262
3.774AsnSer: 3.774 ± 0.454
2.879AsnThr: 2.879 ± 0.283
2.801AsnVal: 2.801 ± 0.361
0.545AsnTrp: 0.545 ± 0.162
1.595AsnTyr: 1.595 ± 0.257
0.0AsnXaa: 0.0 ± 0.0
Pro
3.112ProAla: 3.112 ± 0.423
0.584ProCys: 0.584 ± 0.153
1.79ProAsp: 1.79 ± 0.259
3.307ProGlu: 3.307 ± 0.394
1.245ProPhe: 1.245 ± 0.204
1.867ProGly: 1.867 ± 0.3
0.778ProHis: 0.778 ± 0.169
1.828ProIle: 1.828 ± 0.283
2.023ProLys: 2.023 ± 0.324
2.723ProLeu: 2.723 ± 0.279
1.128ProMet: 1.128 ± 0.211
1.828ProAsn: 1.828 ± 0.23
1.011ProPro: 1.011 ± 0.209
1.401ProGln: 1.401 ± 0.212
1.284ProArg: 1.284 ± 0.203
2.295ProSer: 2.295 ± 0.298
2.14ProThr: 2.14 ± 0.277
2.49ProVal: 2.49 ± 0.344
0.545ProTrp: 0.545 ± 0.18
1.478ProTyr: 1.478 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
3.774GlnAla: 3.774 ± 0.507
0.311GlnCys: 0.311 ± 0.096
2.451GlnAsp: 2.451 ± 0.322
2.023GlnGlu: 2.023 ± 0.251
1.595GlnPhe: 1.595 ± 0.241
2.918GlnGly: 2.918 ± 0.322
0.7GlnHis: 0.7 ± 0.152
2.529GlnIle: 2.529 ± 0.25
2.295GlnLys: 2.295 ± 0.357
3.501GlnLeu: 3.501 ± 0.39
1.439GlnMet: 1.439 ± 0.197
1.595GlnAsn: 1.595 ± 0.247
1.011GlnPro: 1.011 ± 0.192
1.595GlnGln: 1.595 ± 0.29
1.906GlnArg: 1.906 ± 0.295
2.256GlnSer: 2.256 ± 0.337
1.79GlnThr: 1.79 ± 0.254
2.762GlnVal: 2.762 ± 0.304
0.739GlnTrp: 0.739 ± 0.16
1.401GlnTyr: 1.401 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
3.268ArgAla: 3.268 ± 0.32
1.206ArgCys: 1.206 ± 0.241
2.529ArgAsp: 2.529 ± 0.356
2.879ArgGlu: 2.879 ± 0.337
1.634ArgPhe: 1.634 ± 0.187
3.307ArgGly: 3.307 ± 0.381
1.05ArgHis: 1.05 ± 0.246
2.957ArgIle: 2.957 ± 0.328
3.385ArgLys: 3.385 ± 0.371
4.902ArgLeu: 4.902 ± 0.447
1.401ArgMet: 1.401 ± 0.271
2.373ArgAsn: 2.373 ± 0.309
1.595ArgPro: 1.595 ± 0.242
2.062ArgGln: 2.062 ± 0.284
2.606ArgArg: 2.606 ± 0.317
2.645ArgSer: 2.645 ± 0.34
2.723ArgThr: 2.723 ± 0.287
3.696ArgVal: 3.696 ± 0.302
1.167ArgTrp: 1.167 ± 0.202
1.595ArgTyr: 1.595 ± 0.244
0.0ArgXaa: 0.0 ± 0.0
Ser
3.929SerAla: 3.929 ± 0.44
0.817SerCys: 0.817 ± 0.207
3.423SerAsp: 3.423 ± 0.385
3.89SerGlu: 3.89 ± 0.371
2.606SerPhe: 2.606 ± 0.31
6.03SerGly: 6.03 ± 0.718
1.089SerHis: 1.089 ± 0.199
3.812SerIle: 3.812 ± 0.444
4.396SerLys: 4.396 ± 0.441
5.446SerLeu: 5.446 ± 0.457
1.867SerMet: 1.867 ± 0.294
3.229SerAsn: 3.229 ± 0.409
2.412SerPro: 2.412 ± 0.297
2.568SerGln: 2.568 ± 0.275
3.54SerArg: 3.54 ± 0.338
4.357SerSer: 4.357 ± 0.672
4.202SerThr: 4.202 ± 0.517
4.941SerVal: 4.941 ± 0.558
1.128SerTrp: 1.128 ± 0.21
2.256SerTyr: 2.256 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
4.474ThrAla: 4.474 ± 0.416
0.661ThrCys: 0.661 ± 0.139
2.84ThrAsp: 2.84 ± 0.33
3.735ThrGlu: 3.735 ± 0.345
2.606ThrPhe: 2.606 ± 0.349
5.408ThrGly: 5.408 ± 0.533
1.05ThrHis: 1.05 ± 0.229
3.89ThrIle: 3.89 ± 0.359
3.618ThrLys: 3.618 ± 0.401
4.902ThrLeu: 4.902 ± 0.371
1.439ThrMet: 1.439 ± 0.228
3.073ThrAsn: 3.073 ± 0.381
2.918ThrPro: 2.918 ± 0.329
2.49ThrGln: 2.49 ± 0.322
2.334ThrArg: 2.334 ± 0.33
4.046ThrSer: 4.046 ± 0.447
3.774ThrThr: 3.774 ± 0.526
4.435ThrVal: 4.435 ± 0.52
0.584ThrTrp: 0.584 ± 0.169
2.373ThrTyr: 2.373 ± 0.299
0.0ThrXaa: 0.0 ± 0.0
Val
6.302ValAla: 6.302 ± 0.524
1.011ValCys: 1.011 ± 0.22
4.785ValAsp: 4.785 ± 0.4
4.629ValGlu: 4.629 ± 0.515
2.879ValPhe: 2.879 ± 0.334
4.707ValGly: 4.707 ± 0.532
0.973ValHis: 0.973 ± 0.171
3.307ValIle: 3.307 ± 0.381
4.707ValLys: 4.707 ± 0.424
4.746ValLeu: 4.746 ± 0.471
1.556ValMet: 1.556 ± 0.23
3.385ValAsn: 3.385 ± 0.352
2.179ValPro: 2.179 ± 0.311
2.762ValGln: 2.762 ± 0.297
3.19ValArg: 3.19 ± 0.348
3.968ValSer: 3.968 ± 0.433
4.824ValThr: 4.824 ± 0.485
4.785ValVal: 4.785 ± 0.555
1.05ValTrp: 1.05 ± 0.229
3.073ValTyr: 3.073 ± 0.314
0.0ValXaa: 0.0 ± 0.0
Trp
1.401TrpAla: 1.401 ± 0.247
0.156TrpCys: 0.156 ± 0.078
1.284TrpAsp: 1.284 ± 0.19
1.089TrpGlu: 1.089 ± 0.253
0.584TrpPhe: 0.584 ± 0.131
0.778TrpGly: 0.778 ± 0.195
0.584TrpHis: 0.584 ± 0.142
1.089TrpIle: 1.089 ± 0.182
1.439TrpLys: 1.439 ± 0.203
2.179TrpLeu: 2.179 ± 0.308
0.311TrpMet: 0.311 ± 0.108
0.622TrpAsn: 0.622 ± 0.151
0.506TrpPro: 0.506 ± 0.16
0.739TrpGln: 0.739 ± 0.159
0.778TrpArg: 0.778 ± 0.165
0.7TrpSer: 0.7 ± 0.144
0.584TrpThr: 0.584 ± 0.142
1.245TrpVal: 1.245 ± 0.245
0.35TrpTrp: 0.35 ± 0.112
0.545TrpTyr: 0.545 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.373TyrAla: 2.373 ± 0.333
0.428TyrCys: 0.428 ± 0.122
2.451TyrAsp: 2.451 ± 0.301
2.957TyrGlu: 2.957 ± 0.318
1.401TyrPhe: 1.401 ± 0.245
2.762TyrGly: 2.762 ± 0.308
0.7TyrHis: 0.7 ± 0.178
1.751TyrIle: 1.751 ± 0.299
2.606TyrLys: 2.606 ± 0.31
2.373TyrLeu: 2.373 ± 0.329
0.545TyrMet: 0.545 ± 0.148
2.179TyrAsn: 2.179 ± 0.292
1.478TyrPro: 1.478 ± 0.232
1.167TyrGln: 1.167 ± 0.2
1.751TyrArg: 1.751 ± 0.229
2.84TyrSer: 2.84 ± 0.419
2.684TyrThr: 2.684 ± 0.31
1.984TyrVal: 1.984 ± 0.253
0.778TyrTrp: 0.778 ± 0.158
1.089TyrTyr: 1.089 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 161 proteins (25706 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski