Bifidobacterium callitrichidarum
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2443 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2NAB3|A0A2U2NAB3_9BIFI Uncharacterized protein OS=Bifidobacterium callitrichidarum OX=2052941 GN=DF196_05880 PE=4 SV=1
MM1 pKa = 7.68 TDD3 pKa = 2.86 NEE5 pKa = 5.39 AIFKK9 pKa = 10.44 DD10 pKa = 4.49 LILAHH15 pKa = 6.67 IRR17 pKa = 11.84 EE18 pKa = 4.29 QLASHH23 pKa = 7.25 FSNGDD28 pKa = 3.27 PVLVRR33 pKa = 11.84 NYY35 pKa = 10.22 VDD37 pKa = 4.34 DD38 pKa = 4.65 AGGYY42 pKa = 9.32 YY43 pKa = 10.03 DD44 pKa = 5.28 YY45 pKa = 11.73 SPIDD49 pKa = 3.78 PSSISLDD56 pKa = 3.63 DD57 pKa = 3.99 CQLIAWEE64 pKa = 5.55 DD65 pKa = 3.27 ITDD68 pKa = 4.06 PTDD71 pKa = 3.35 QQPDD75 pKa = 3.21 RR76 pKa = 11.84 CACSLITLSTDD87 pKa = 2.66 GGEE90 pKa = 4.15 PFVVLLKK97 pKa = 9.96 GTSLGDD103 pKa = 3.59 MLRR106 pKa = 11.84 DD107 pKa = 3.48 WTGLDD112 pKa = 3.6 YY113 pKa = 11.65 VDD115 pKa = 4.3
Molecular weight: 12.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.478
ProMoST 3.859
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.164
Thurlkill 3.567
EMBOSS 3.732
Sillero 3.846
Patrickios 0.947
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.779
Protein with the highest isoelectric point:
>tr|A0A2U2MXN8|A0A2U2MXN8_9BIFI Uncharacterized protein (Fragment) OS=Bifidobacterium callitrichidarum OX=2052941 GN=DF196_13055 PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 HH13 pKa = 4.82 MKK15 pKa = 9.64 HH16 pKa = 5.7 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 SGRR28 pKa = 11.84 AVINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.06 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.705
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.427
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2443
0
2443
852745
29
3229
349.1
38.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.061 ± 0.069
0.901 ± 0.014
6.584 ± 0.043
5.63 ± 0.053
3.422 ± 0.03
8.131 ± 0.045
2.061 ± 0.025
5.28 ± 0.037
4.09 ± 0.047
8.743 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.581 ± 0.023
3.433 ± 0.032
4.703 ± 0.036
3.358 ± 0.031
5.765 ± 0.062
5.993 ± 0.046
6.343 ± 0.048
7.604 ± 0.048
1.468 ± 0.021
2.852 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here