Frondihabitans sp. 762G35

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Frondihabitans; unclassified Frondihabitans

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R2W6A4|A0A2R2W6A4_9MICO Transposon Tn10 TetC protein OS=Frondihabitans sp. 762G35 OX=1446794 GN=tetC PE=4 SV=1
MM1 pKa = 7.28GLKK4 pKa = 10.32PIVYY8 pKa = 8.11YY9 pKa = 9.87TALCDD14 pKa = 4.81EE15 pKa = 5.39PGCDD19 pKa = 3.93ADD21 pKa = 5.92LVDD24 pKa = 5.13DD25 pKa = 4.43HH26 pKa = 6.69TDD28 pKa = 3.32EE29 pKa = 4.75YY30 pKa = 11.5GSTTLPEE37 pKa = 4.13LHH39 pKa = 6.67IMAGDD44 pKa = 4.26AEE46 pKa = 4.38WQQIPIGATTYY57 pKa = 8.88WYY59 pKa = 10.03CDD61 pKa = 2.85KK62 pKa = 10.84HH63 pKa = 7.51HH64 pKa = 7.38RR65 pKa = 11.84EE66 pKa = 4.27GLQPTPDD73 pKa = 3.48VNDD76 pKa = 3.81PTEE79 pKa = 4.37LPPPLFTIEE88 pKa = 4.23TPP90 pKa = 3.43

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R2WAY8|A0A2R2WAY8_9MICO HTH-type transcriptional repressor OS=Frondihabitans sp. 762G35 OX=1446794 GN=AS850_11230 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3211

0

3211

1020046

29

2980

317.7

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.086 ± 0.064

0.454 ± 0.009

6.157 ± 0.044

5.239 ± 0.046

3.178 ± 0.028

9.02 ± 0.041

1.881 ± 0.022

4.18 ± 0.035

2.117 ± 0.032

10.145 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.539 ± 0.015

1.964 ± 0.027

5.4 ± 0.037

2.696 ± 0.024

7.325 ± 0.053

6.317 ± 0.039

6.632 ± 0.063

9.339 ± 0.039

1.389 ± 0.02

1.942 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski