Tubulinosema ratisbonensis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Tubulinosematoidea; Tubulinosematidae; Tubulinosema

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2996 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A437AJ02|A0A437AJ02_9MICR Uncharacterized protein OS=Tubulinosema ratisbonensis OX=291195 GN=TUBRATIS_23960 PE=4 SV=1
MM1 pKa = 7.2MLITQQLLLLILCKK15 pKa = 10.13ILLFKK20 pKa = 10.85ARR22 pKa = 11.84EE23 pKa = 4.02IYY25 pKa = 10.58EE26 pKa = 4.76DD27 pKa = 3.79DD28 pKa = 4.28AKK30 pKa = 11.29SLSQSDD36 pKa = 4.12LIEE39 pKa = 3.94YY40 pKa = 10.24LNNLNEE46 pKa = 4.03EE47 pKa = 4.19GMIEE51 pKa = 4.03LLSSISEE58 pKa = 4.1MTTGQGEE65 pKa = 4.32DD66 pKa = 3.58SNEE69 pKa = 4.17EE70 pKa = 3.71EE71 pKa = 4.45SSTYY75 pKa = 9.99EE76 pKa = 4.01YY77 pKa = 10.59PNLYY81 pKa = 8.95PFSVRR86 pKa = 11.84TLPEE90 pKa = 4.26PLHH93 pKa = 7.31DD94 pKa = 5.66DD95 pKa = 3.51FDD97 pKa = 4.45FRR99 pKa = 11.84NSINPQNQEE108 pKa = 4.19KK109 pKa = 10.49NDD111 pKa = 3.69SNLIIEE117 pKa = 4.72NGPTSFNLTTTVVQSNLPTKK137 pKa = 9.95CYY139 pKa = 10.5CEE141 pKa = 3.94LVYY144 pKa = 10.34IDD146 pKa = 6.33DD147 pKa = 4.67SDD149 pKa = 4.7CDD151 pKa = 3.67SDD153 pKa = 3.76NN154 pKa = 3.59

Molecular weight: 17.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A437AM55|A0A437AM55_9MICR Uncharacterized protein OS=Tubulinosema ratisbonensis OX=291195 GN=TUBRATIS_13460 PE=4 SV=1
MM1 pKa = 7.77SAMQYY6 pKa = 10.38LRR8 pKa = 11.84EE9 pKa = 3.94IRR11 pKa = 11.84KK12 pKa = 9.81KK13 pKa = 10.59KK14 pKa = 10.68LSDD17 pKa = 3.49LNRR20 pKa = 11.84YY21 pKa = 9.51LYY23 pKa = 9.83TIRR26 pKa = 11.84CYY28 pKa = 8.49QFRR31 pKa = 11.84LSTAVHH37 pKa = 6.34KK38 pKa = 10.62ADD40 pKa = 3.44KK41 pKa = 8.79PTYY44 pKa = 9.16IDD46 pKa = 3.21KK47 pKa = 10.83ARR49 pKa = 11.84QLGYY53 pKa = 9.83KK54 pKa = 9.89AKK56 pKa = 9.66EE57 pKa = 4.23GYY59 pKa = 9.69SIYY62 pKa = 10.29RR63 pKa = 11.84VRR65 pKa = 11.84IKK67 pKa = 10.69RR68 pKa = 11.84GGRR71 pKa = 11.84KK72 pKa = 9.39KK73 pKa = 10.58LVNNGNTRR81 pKa = 11.84GKK83 pKa = 9.97CVNAGIYY90 pKa = 7.14EE91 pKa = 4.17QKK93 pKa = 10.49PSLNLQGMAEE103 pKa = 3.94IKK105 pKa = 10.27LGKK108 pKa = 9.81KK109 pKa = 9.1IRR111 pKa = 11.84NLRR114 pKa = 11.84ILNSYY119 pKa = 8.47WVGQDD124 pKa = 3.08GTYY127 pKa = 10.34KK128 pKa = 9.16YY129 pKa = 10.78YY130 pKa = 10.72EE131 pKa = 4.45VIMVDD136 pKa = 3.86PLCQRR141 pKa = 11.84IRR143 pKa = 11.84DD144 pKa = 3.88DD145 pKa = 4.98PRR147 pKa = 11.84INWICNPVMKK157 pKa = 10.36HH158 pKa = 5.0RR159 pKa = 11.84EE160 pKa = 4.2CRR162 pKa = 11.84GLTSATKK169 pKa = 10.2KK170 pKa = 10.66SRR172 pKa = 11.84GLGKK176 pKa = 10.47GIRR179 pKa = 11.84YY180 pKa = 7.81NQTIGGSRR188 pKa = 11.84YY189 pKa = 9.88SCWKK193 pKa = 9.72RR194 pKa = 11.84RR195 pKa = 11.84NTVSLKK201 pKa = 10.36KK202 pKa = 10.72YY203 pKa = 9.92RR204 pKa = 3.66

Molecular weight: 23.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2996

0

2996

936452

41

3156

312.6

36.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.722 ± 0.044

1.778 ± 0.02

4.884 ± 0.03

7.943 ± 0.058

7.093 ± 0.052

3.228 ± 0.045

1.548 ± 0.017

9.066 ± 0.042

11.066 ± 0.052

10.753 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.879 ± 0.015

7.72 ± 0.045

2.606 ± 0.034

2.735 ± 0.024

3.092 ± 0.029

6.988 ± 0.035

4.667 ± 0.032

4.811 ± 0.029

0.483 ± 0.009

4.443 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski